FastQCFastQC Report
Mon 6 Jun 2016
ERR1211318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10347323
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299220.2891762439425154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234630.22675430157152723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132220.12778184270462997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159250.048.674861
GTATCAA290950.042.8243261
CAACGCA347400.035.182415
ATCAACG350150.035.0141373
TATCAAC366100.033.737222
TCAACGC366550.033.5499534
AACGCAG373800.032.9482466
ACGCAGA421250.028.915677
CGCAGAG432500.028.0419738
GTACATG388050.026.2662561
TACATGG396550.025.4296952
GTACTAG40450.025.3492371
GCAGAGT472000.024.8207159
ACATGGG401900.024.0981393
GTGGTAT77150.024.0207861
GAGTACT267550.021.57780812-13
CATGGGG263850.021.0692484
CAGAGTA464700.020.5747610-11
TGGTATC82150.020.4969752
AGAGTAC441200.019.90781410-11
Produced by FastQC (version 0.11.5)