FastQCFastQC Report
Mon 6 Jun 2016
ERR1211318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10347323
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269010.25998028668864404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225730.21815304306244235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128190.1238871155370331No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172200.049.9829251
GTATCAA300550.039.9741021
ATCAACG365050.032.2477043
CAACGCA366100.032.124155
TATCAAC385950.031.0336252
TCAACGC382850.030.8098244
AACGCAG391700.030.383936
ACGCAGA439500.026.695127
CGCAGAG451500.025.9544458
GTGGTAT85000.024.2382371
GCAGAGT500700.023.178799
GTACATG393250.022.8775141
TGGTATC87850.022.376132
TACATGG402000.022.1037852
GTACTAG41950.022.0388891
ACATGGG404700.021.1930523
GAGTACT274900.021.02952212-13
CAGAGTA493600.019.93202410-11
AGAGTAC467100.019.6770510-11
GTACTTT294700.019.4654114-15
Produced by FastQC (version 0.11.5)