##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10347323 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12226031795857 33.0 31.0 34.0 30.0 34.0 2 32.25938602670468 34.0 31.0 34.0 30.0 34.0 3 32.3244446897038 34.0 31.0 34.0 30.0 34.0 4 35.95866505761925 37.0 35.0 37.0 35.0 37.0 5 35.86660743073353 37.0 35.0 37.0 35.0 37.0 6 35.879076742844504 37.0 35.0 37.0 35.0 37.0 7 35.7953312175526 37.0 35.0 37.0 33.0 37.0 8 35.77489308104135 37.0 35.0 37.0 33.0 37.0 9 37.38924405858404 39.0 37.0 39.0 34.0 39.0 10-11 37.34417365728314 39.0 37.0 39.0 34.0 39.0 12-13 37.282918103552 39.0 37.0 39.0 34.0 39.0 14-15 38.53082555748961 40.0 38.0 41.0 34.0 41.0 16-17 38.518404422090626 40.0 38.0 41.0 34.0 41.0 18-19 38.52281218050311 40.0 38.0 41.0 34.0 41.0 20-21 38.490972399334595 40.0 38.0 41.0 34.0 41.0 22-23 38.3910939573453 40.0 38.0 41.0 34.0 41.0 24-25 38.30938064850203 40.0 38.0 41.0 34.0 41.0 26-27 38.217859633839595 40.0 38.0 41.0 33.5 41.0 28-29 38.11830108135215 40.0 38.0 41.0 33.0 41.0 30-31 38.12655157280777 40.0 38.0 41.0 33.0 41.0 32-33 38.07028175306792 40.0 37.0 41.0 33.0 41.0 34-35 37.95021006882649 40.0 37.0 41.0 33.0 41.0 36-37 37.81346112419608 40.0 37.0 41.0 33.0 41.0 38-39 37.704614903777525 40.0 37.0 41.0 33.0 41.0 40-41 37.60193481927645 40.0 37.0 41.0 32.5 41.0 42-43 37.50426506450026 40.0 37.0 41.0 32.0 41.0 44-45 37.35603546927065 40.0 36.0 41.0 31.5 41.0 46-47 37.1313272041474 39.5 36.0 41.0 31.0 41.0 48-49 35.59005087596086 39.0 35.0 41.0 25.0 41.0 50-51 36.33758407850997 39.0 35.0 40.5 29.0 41.0 52-53 36.44097246215277 39.0 35.0 40.0 30.0 41.0 54-55 36.28618068654086 39.0 35.0 40.0 29.0 41.0 56-57 36.083894790952215 38.0 35.0 40.0 29.0 41.0 58-59 35.85884851569821 38.0 35.0 40.0 28.5 41.0 60-61 35.61540062101086 38.0 34.0 40.0 28.0 41.0 62-63 35.30771524190364 37.0 34.0 40.0 28.0 41.0 64-65 34.98359938121193 37.0 34.0 40.0 27.5 41.0 66-67 34.656781710593165 36.0 33.5 39.0 27.0 41.0 68-69 34.302000430449496 36.0 33.0 39.0 26.0 40.0 70-71 33.94493870540235 35.0 33.0 38.5 26.0 40.0 72-73 33.56567795361177 35.0 32.5 38.0 26.0 40.0 74-75 33.196119952957886 35.0 32.0 37.0 26.0 39.0 76-77 32.24674024382925 34.0 31.0 36.0 24.5 39.0 78-79 32.6699484977902 35.0 32.0 36.0 26.0 38.5 80-81 32.53192274948796 35.0 32.0 36.0 25.5 37.5 82-83 32.250578144704676 34.5 32.0 35.5 25.0 37.0 84-85 31.957244013741523 34.0 31.0 35.0 25.0 37.0 86-87 31.689694619564886 34.0 31.0 35.0 24.0 36.0 88-89 31.45795729001598 34.0 31.0 35.0 24.0 36.0 90-91 31.23368367837749 34.0 31.0 35.0 23.0 35.5 92-93 31.042728152972515 34.0 30.5 35.0 23.0 35.0 94-95 30.84661747777662 34.0 30.0 35.0 22.0 35.0 96-97 30.658475820267718 34.0 30.0 35.0 20.0 35.0 98-99 30.445824828315498 34.0 29.5 35.0 20.0 35.0 100 28.025123116384787 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2561148871708099 1101 2 -0.26493769597686523 1101 3 -0.25135902154357126 1101 4 -0.15678505543094445 1101 5 -0.1883880309817627 1101 6 -0.22078252720351088 1101 7 -0.20906268127789218 1101 8 -0.20550119806762268 1101 9 -0.29842436677667195 1101 10-11 -0.3054959734233904 1101 12-13 -0.4255559677511016 1101 14-15 -0.4607517977621143 1101 16-17 -0.4980335429405045 1101 18-19 -0.5124338125629322 1101 20-21 -0.5255843351950702 1101 22-23 -0.6402742332009836 1101 24-25 -0.5839601376030998 1101 26-27 -0.6285359230404879 1101 28-29 -0.5795914285018284 1101 30-31 -0.567226854451043 1101 32-33 -0.5511909150263676 1101 34-35 -0.6041333418360395 1101 36-37 -0.638768844433308 1101 38-39 -0.5694159073770209 1101 40-41 -0.5751851251795372 1101 42-43 -0.6472204333475204 1101 44-45 -0.5846039227002322 1101 46-47 -0.6790605468731172 1101 48-49 0.572306688841671 1101 50-51 -0.3249314363892779 1101 52-53 -0.6279533869772038 1101 54-55 -0.6280805302929977 1101 56-57 -0.586896111101531 1101 58-59 -0.7591875394834418 1101 60-61 -0.7335465656810527 1101 62-63 -0.7507749847483112 1101 64-65 -0.7871885368638445 1101 66-67 -0.7522956375432628 1101 68-69 -0.781598421893122 1101 70-71 -0.7239578853198623 1101 72-73 -0.9133327845375518 1101 74-75 -0.8669350118402903 1101 76-77 -0.8125052367202201 1101 78-79 -0.7362106362743006 1101 80-81 -0.5818785222895144 1101 82-83 -0.613469445616694 1101 84-85 -0.6565894443610176 1101 86-87 -0.6644948088862321 1101 88-89 -0.6720119962746587 1101 90-91 -0.6556627904615482 1101 92-93 -0.6925177011362429 1101 94-95 -0.75034994484216 1101 96-97 -0.9079241593947813 1101 98-99 -1.0343065785906482 1101 100 -0.8526162764719842 1102 1 -0.2806399730386211 1102 2 -0.2701278996844856 1102 3 -0.31378863845150207 1102 4 -0.24443265403490955 1102 5 -0.2223230203270461 1102 6 -0.2367112883857132 1102 7 -0.22736144612013476 1102 8 -0.24101601657152827 1102 9 -0.32731379341552014 1102 10-11 -0.37600751786935405 1102 12-13 -0.45090741944827073 1102 14-15 -0.5050397218861988 1102 16-17 -0.5222007669234827 1102 18-19 -0.5255278915158996 1102 20-21 -0.7009741610008149 1102 22-23 -0.6772483617638088 1102 24-25 -0.6659504016216928 1102 26-27 -0.5729081060004688 1102 28-29 -0.595617824337495 1102 30-31 -0.759701891390776 1102 32-33 -0.6739953967931669 1102 34-35 -0.6416967590156872 1102 36-37 -0.664930620245535 1102 38-39 -0.6866551686924041 1102 40-41 -0.595281375691286 1102 42-43 -0.6556464412483152 1102 44-45 -0.6829201936353044 1102 46-47 -0.7083852243784392 1102 48-49 0.5640836801051989 1102 50-51 -0.33025657357249116 1102 52-53 -0.6014833661498784 1102 54-55 -0.7657210042060356 1102 56-57 -0.7466381345695865 1102 58-59 -0.9020467124775351 1102 60-61 -0.7490648533539996 1102 62-63 -0.7756933666644983 1102 64-65 -0.8127749185909394 1102 66-67 -0.7298077307299948 1102 68-69 -0.7664085062470036 1102 70-71 -0.779606563934081 1102 72-73 -0.832370077358874 1102 74-75 -0.9963025154678107 1102 76-77 -0.8471746416248322 1102 78-79 -0.6569296646710079 1102 80-81 -0.6332190089579441 1102 82-83 -0.6303080641288936 1102 84-85 -0.5546384453057662 1102 86-87 -0.5472861166706302 1102 88-89 -0.6482186815412767 1102 90-91 -0.6662254880298946 1102 92-93 -0.8482803567237802 1102 94-95 -0.8214008559092569 1102 96-97 -0.8493899442263597 1102 98-99 -0.8789645786783247 1102 100 -0.8648031764227362 1103 1 -0.3576199593049978 1103 2 -0.3108547853141559 1103 3 -0.3380563483393608 1103 4 -0.22759551547910917 1103 5 -0.29622149572142575 1103 6 -0.2861405520960929 1103 7 -0.27610251071958203 1103 8 -0.34196785718572187 1103 9 -0.42732977774095104 1103 10-11 -0.42873654897099556 1103 12-13 -0.4904918566616274 1103 14-15 -0.567449810055642 1103 16-17 -0.5756215295521372 1103 18-19 -0.6289631918343233 1103 20-21 -0.6196666112232734 1103 22-23 -0.7243034652290774 1103 24-25 -0.7758162799489625 1103 26-27 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-0.9868984024506382 1103 92-93 -0.8590604117781133 1103 94-95 -0.9323697668242907 1103 96-97 -1.0729837615737416 1103 98-99 -1.0688998521469593 1103 100 -1.0359817085549103 1104 1 -0.3096602574782885 1104 2 -0.2987386580559708 1104 3 -0.335700981392673 1104 4 -0.1645589256712796 1104 5 -0.20729897892451987 1104 6 -0.30557057219855466 1104 7 -0.2575025669730877 1104 8 -0.2775504278337593 1104 9 -0.44320884390533166 1104 10-11 -0.36557372593193094 1104 12-13 -0.3707464820609374 1104 14-15 -0.49554332710776094 1104 16-17 -0.5948798061719529 1104 18-19 -0.5855196281462014 1104 20-21 -0.5984643828438792 1104 22-23 -0.578435457758637 1104 24-25 -0.6912120025567745 1104 26-27 -0.68411005903301 1104 28-29 -0.6841941911448544 1104 30-31 -0.6548183022454594 1104 32-33 -0.6013355919627941 1104 34-35 -0.6349995335159733 1104 36-37 -0.6510533870279218 1104 38-39 -0.5715721704185768 1104 40-41 -0.6960500842662043 1104 42-43 -0.6478324183955309 1104 44-45 -0.6942897123596694 1104 46-47 -0.6882643135749404 1104 48-49 0.5246270004057578 1104 50-51 -0.31738894898300885 1104 52-53 -0.6132569529039387 1104 54-55 -0.7643971980343593 1104 56-57 -0.8077670984881848 1104 58-59 -0.8429108432073136 1104 60-61 -0.86119671759716 1104 62-63 -0.7928229260176707 1104 64-65 -0.8241122477372969 1104 66-67 -0.8067976539060666 1104 68-69 -0.8293375502496048 1104 70-71 -0.7800605811163166 1104 72-73 -0.8435204365648445 1104 74-75 -0.8236616802540695 1104 76-77 -0.8076316668175494 1104 78-79 -0.6332994641742928 1104 80-81 -0.608046300866512 1104 82-83 -0.6926250927463791 1104 84-85 -0.6675475912106776 1104 86-87 -0.6351242851705692 1104 88-89 -0.6873336500436444 1104 90-91 -0.8797189088533308 1104 92-93 -0.7796142047924128 1104 94-95 -0.7795755551065326 1104 96-97 -0.8681042900229627 1104 98-99 -0.881191380165653 1104 100 -0.8132942539208692 1105 1 -0.2652310079446778 1105 2 -0.28947170316752135 1105 3 -0.30827758797911287 1105 4 -0.19860245499116758 1105 5 -0.2365929094303567 1105 6 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1105 68-69 -0.7480212844609611 1105 70-71 -0.8225134085103889 1105 72-73 -0.7142703247607258 1105 74-75 -0.9022289942344699 1105 76-77 -0.7830816683941393 1105 78-79 -0.6522069284107417 1105 80-81 -0.6538081923300254 1105 82-83 -0.6045785052126647 1105 84-85 -0.5905260935947751 1105 86-87 -0.6164623445617359 1105 88-89 -0.7118973078685507 1105 90-91 -0.7938349501757749 1105 92-93 -0.7385769686495358 1105 94-95 -0.7516143872160761 1105 96-97 -0.7255596363423322 1105 98-99 -0.808826789433251 1105 100 -0.8207124060739623 1106 1 -0.3033396240476982 1106 2 -0.2586799946954983 1106 3 -0.31329028773163614 1106 4 -0.20812550367694627 1106 5 -0.23081205251151715 1106 6 -0.325477864033914 1106 7 -0.2556355421705305 1106 8 -0.3056997609630159 1106 9 -0.4257352808497359 1106 10-11 -0.4092157905382905 1106 12-13 -0.45195666187094474 1106 14-15 -0.6145290954584155 1106 16-17 -0.5241198383506003 1106 18-19 -0.556791465465416 1106 20-21 -0.55951765985062 1106 22-23 -0.6172547754103945 1106 24-25 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1.1926208633370479 2311 1 0.3326225006184913 2311 2 0.3644562077800657 2311 3 0.3685384144961219 2311 4 0.26172732321367675 2311 5 0.3041095496035524 2311 6 0.35333842798043946 2311 7 0.35829956105467886 2311 8 0.16200367652606928 2311 9 0.44250419180875156 2311 10-11 0.37027684760280266 2311 12-13 0.4694571275001067 2311 14-15 0.4383774254465109 2311 16-17 0.4245239240431289 2311 18-19 0.5729217515298259 2311 20-21 0.6139673271048807 2311 22-23 0.6167145159816911 2311 24-25 0.569170756645839 2311 26-27 0.5991838676485273 2311 28-29 0.6760223039667537 2311 30-31 0.5559095791988256 2311 32-33 0.574854946639789 2311 34-35 0.7058938265058288 2311 36-37 0.7542128822652643 2311 38-39 0.8134898674780189 2311 40-41 0.7162559584685795 2311 42-43 0.7605737028361048 2311 44-45 0.8221859275538534 2311 46-47 0.8520930064534653 2311 48-49 -6.093427571719616 2311 50-51 -1.067480756311184 2311 52-53 0.34812647101396976 2311 54-55 0.8038941805630841 2311 56-57 0.8739490651096631 2311 58-59 0.912850629223307 2311 60-61 0.7420548106951017 2311 62-63 0.6676218989472389 2311 64-65 0.7427030274268702 2311 66-67 0.8617467424880871 2311 68-69 0.8707649910816713 2311 70-71 0.952556183889854 2311 72-73 0.46215755220277543 2311 74-75 0.6743959715131354 2311 76-77 0.8011667637236926 2311 78-79 0.7108462390133212 2311 80-81 0.747664207245208 2311 82-83 0.8066381147671393 2311 84-85 0.8748081776219827 2311 86-87 0.8929805056687918 2311 88-89 0.9416982313080915 2311 90-91 0.9486426629690037 2311 92-93 0.9776489292682093 2311 94-95 0.9851473770753678 2311 96-97 0.9866132326995682 2311 98-99 1.0374153580105165 2311 100 1.1560938653564357 2312 1 0.04355990404669541 2312 2 0.2210892254933654 2312 3 0.19770470110181293 2312 4 6.205525496980613E-4 2312 5 0.19817135771105399 2312 6 0.13246036934549466 2312 7 0.2899111055861283 2312 8 0.3133084225114189 2312 9 0.231435213972901 2312 10-11 0.22606781366117445 2312 12-13 0.2692517163831525 2312 14-15 0.3290779827341055 2312 16-17 0.45181557454799304 2312 18-19 0.32928585935593446 2312 20-21 0.33424261991178383 2312 22-23 0.5809462535416046 2312 24-25 0.6902576913190828 2312 26-27 0.6239513660941469 2312 28-29 0.7142232898621117 2312 30-31 0.6960882761159226 2312 32-33 0.7262730093737133 2312 34-35 0.37719153348837153 2312 36-37 0.6433378754830912 2312 38-39 0.7648032291141291 2312 40-41 0.5636016961221344 2312 42-43 0.5772253901545881 2312 44-45 0.7013613945524995 2312 46-47 0.7477328235084215 2312 48-49 -4.712778439785886 2312 50-51 -0.7686784615524971 2312 52-53 0.5393042852635759 2312 54-55 0.8008167951260887 2312 56-57 0.865435152128029 2312 58-59 0.8829099835575533 2312 60-61 0.9077661405277055 2312 62-63 0.7136737027976352 2312 64-65 0.9158262196993832 2312 66-67 0.8505890503184972 2312 68-69 0.7437135861146373 2312 70-71 0.8258709774034827 2312 72-73 0.9029022302372098 2312 74-75 0.587877674616756 2312 76-77 0.7738291621460363 2312 78-79 0.4592121101259039 2312 80-81 0.6831452354924323 2312 82-83 0.6521138086209746 2312 84-85 0.7220018523017764 2312 86-87 0.832861970000824 2312 88-89 0.838747630844022 2312 90-91 0.6110094001225086 2312 92-93 0.7896502978665403 2312 94-95 0.922293212689592 2312 96-97 1.0440637789273381 2312 98-99 1.0725843974151985 2312 100 0.6473815559872662 2313 1 0.09991048570604022 2313 2 0.27001423349481257 2313 3 0.3123431583260725 2313 4 0.09636887168446862 2313 5 0.1874980765947356 2313 6 0.2663646906187864 2313 7 0.14801147541614057 2313 8 0.2535913846373248 2313 9 0.46675084656382637 2313 10-11 0.21867956084005158 2313 12-13 0.02191964636519117 2313 14-15 0.10560217639727654 2313 16-17 0.09768283090684804 2313 18-19 0.41358974072858956 2313 20-21 0.35477121798222555 2313 22-23 0.4290588796835024 2313 24-25 0.23944264418779682 2313 26-27 0.4064281453184506 2313 28-29 0.43193559021919015 2313 30-31 0.5806289011301686 2313 32-33 0.47622862099576935 2313 34-35 0.3660884614806861 2313 36-37 -0.4265750434083131 2313 38-39 0.14274485929727376 2313 40-41 0.3651684736547267 2313 42-43 0.5250032159713314 2313 44-45 0.4904233710797641 2313 46-47 0.377358750720596 2313 48-49 -5.49358945230054 2313 50-51 -1.4225423277982117 2313 52-53 0.07228307088194441 2313 54-55 0.25790430262190256 2313 56-57 0.2422844780013378 2313 58-59 0.40838904611101867 2313 60-61 0.6652849354051895 2313 62-63 0.7378375135213204 2313 64-65 0.7726552549081447 2313 66-67 0.7974348256841566 2313 68-69 0.7916677038343067 2313 70-71 0.7372468593130606 2313 72-73 0.5379815566005419 2313 74-75 0.7005225226422667 2313 76-77 0.6956171004116527 2313 78-79 0.6822996588033305 2313 80-81 -0.32035098218457136 2313 82-83 0.15473694234706414 2313 84-85 0.5328904353353003 2313 86-87 0.642953214840535 2313 88-89 0.6460269436714263 2313 90-91 0.643046887711062 2313 92-93 0.7761891565721477 2313 94-95 0.6942100235247217 2313 96-97 0.7379963349220944 2313 98-99 0.885212643399722 2313 100 0.9358972221310786 2314 1 0.0644306463546016 2314 2 0.18145337127315742 2314 3 0.22664711194121878 2314 4 0.07124296159451404 2314 5 0.22935417118324608 2314 6 0.27892153233096906 2314 7 0.08859987534540181 2314 8 0.2380339352977643 2314 9 0.43722914136875346 2314 10-11 0.2946221918371279 2314 12-13 0.19360421754009138 2314 14-15 0.2089925872494618 2314 16-17 0.4166965817365025 2314 18-19 0.5184770548092175 2314 20-21 0.5756409555923625 2314 22-23 0.2829185818262019 2314 24-25 0.413884377584786 2314 26-27 0.45235771287434545 2314 28-29 0.56876731766679 2314 30-31 0.4971091731455317 2314 32-33 0.486485628314 2314 34-35 0.34329647586205425 2314 36-37 0.42839095087971657 2314 38-39 0.46170360065989513 2314 40-41 0.26244122956396154 2314 42-43 0.28265890192249543 2314 44-45 0.5454919395118552 2314 46-47 0.6419659558230464 2314 48-49 -6.334398943612577 2314 50-51 -1.509246158147235 2314 52-53 -0.7765834070233524 2314 54-55 -0.3355192701479197 2314 56-57 -0.03265428409130067 2314 58-59 0.39044853752833575 2314 60-61 0.642367489598584 2314 62-63 0.4829489634384956 2314 64-65 0.6792361355363283 2314 66-67 0.7591699315910958 2314 68-69 0.766380201022244 2314 70-71 0.5552868741434054 2314 72-73 0.7475311600705439 2314 74-75 0.7848320005765501 2314 76-77 0.7310319355935277 2314 78-79 0.5485111603532573 2314 80-81 0.5420459470946852 2314 82-83 0.627469098721825 2314 84-85 0.5920477705948777 2314 86-87 0.6004792454958015 2314 88-89 0.5511602769336399 2314 90-91 0.7460155452784853 2314 92-93 0.8265003553920742 2314 94-95 0.9059841361446352 2314 96-97 0.8796026559987133 2314 98-99 0.9233895784955557 2314 100 -0.5054946915200809 2315 1 0.11104302000824617 2315 2 0.1980471798061032 2315 3 0.2745875793482426 2315 4 0.1054632889086804 2315 5 0.21682842737630637 2315 6 0.23484216983952422 2315 7 0.32025126126678316 2315 8 0.22754801362566468 2315 9 0.3817168229632628 2315 10-11 0.1838244986616644 2315 12-13 0.39506496786367507 2315 14-15 0.17469550690663027 2315 16-17 0.29404969641646517 2315 18-19 0.3739299018839617 2315 20-21 0.3217228746956522 2315 22-23 0.41346981647051706 2315 24-25 0.42031330238770437 2315 26-27 0.49102529291374 2315 28-29 0.5809832315766741 2315 30-31 0.5827542048974621 2315 32-33 0.6118899590633333 2315 34-35 0.6217702198075017 2315 36-37 0.5466957631614306 2315 38-39 0.6138639565920769 2315 40-41 0.6524358764783003 2315 42-43 0.6385507938447077 2315 44-45 0.6434071379993895 2315 46-47 0.5272185728209422 2315 48-49 -4.916353619652412 2315 50-51 -0.9351956807293789 2315 52-53 0.20802975210467878 2315 54-55 0.14523530044237276 2315 56-57 0.5065742239041171 2315 58-59 0.5975564672867648 2315 60-61 0.458801059536853 2315 62-63 0.30957865804274576 2315 64-65 0.4607178446938036 2315 66-67 -0.21114614649316366 2315 68-69 0.4673001053466095 2315 70-71 0.48131873274849113 2315 72-73 0.5678491797610903 2315 74-75 0.7589105752104288 2315 76-77 0.7066798853624263 2315 78-79 0.6403538099154673 2315 80-81 0.4563941793007018 2315 82-83 0.43609195102137477 2315 84-85 0.4535219663668322 2315 86-87 0.4249523377063973 2315 88-89 0.42400904725198885 2315 90-91 0.5465317216626921 2315 92-93 0.6042634784426717 2315 94-95 0.24778477481394745 2315 96-97 0.34908765999898606 2315 98-99 0.30042374053262577 2315 100 0.337477976410991 2316 1 0.2489193467449411 2316 2 0.28828984017371084 2316 3 0.19134386472806142 2316 4 -0.03905994678162017 2316 5 0.15816009181524038 2316 6 0.16054811876008301 2316 7 0.24447043223082687 2316 8 0.30114795316633547 2316 9 0.46314804058923187 2316 10-11 0.4201559506797139 2316 12-13 0.3335199522321659 2316 14-15 0.4390333769799213 2316 16-17 0.38478323481423615 2316 18-19 0.09469565955281212 2316 20-21 0.426869941457646 2316 22-23 0.5533117129361571 2316 24-25 0.5995539418825331 2316 26-27 0.6289596042431143 2316 28-29 0.6458522660375152 2316 30-31 0.5790708898080226 2316 32-33 0.5413016604677097 2316 34-35 0.5326012769189958 2316 36-37 0.4318771348451307 2316 38-39 0.5391215642393448 2316 40-41 0.13748233285623712 2316 42-43 0.07464277110739914 2316 44-45 0.28979880117361034 2316 46-47 0.3822058927662013 2316 48-49 -4.787244309546381 2316 50-51 -0.9106881186158944 2316 52-53 0.2703297031567331 2316 54-55 0.5363763924988518 2316 56-57 0.4634677163516798 2316 58-59 0.3140488635303811 2316 60-61 0.46923052900201867 2316 62-63 0.38186605733386614 2316 64-65 0.4133464094563024 2316 66-67 0.4882245688969178 2316 68-69 0.17659333338275474 2316 70-71 -0.21408570610493172 2316 72-73 -0.08498923300199834 2316 74-75 0.11649253567154005 2316 76-77 0.1381124104634921 2316 78-79 0.280483736485138 2316 80-81 0.23776012833062055 2316 82-83 0.11100723504772958 2316 84-85 0.2746123984954174 2316 86-87 0.3515082646207155 2316 88-89 0.19368932016585916 2316 90-91 0.19531570322091696 2316 92-93 0.28222686198912683 2316 94-95 0.4994673235056517 2316 96-97 0.5837343655052543 2316 98-99 0.5334508138901803 2316 100 -0.2853154437854286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 8.0 11 16.0 12 36.0 13 186.0 14 717.0 15 2126.0 16 5153.0 17 10303.0 18 18210.0 19 28268.0 20 40170.0 21 57237.0 22 72363.0 23 76971.0 24 80564.0 25 88516.0 26 105861.0 27 131512.0 28 162310.0 29 200759.0 30 247919.0 31 307401.0 32 387712.0 33 502218.0 34 692489.0 35 977819.0 36 1526827.0 37 2322168.0 38 2017513.0 39 283937.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5703997503518 20.303667114069047 14.649982076836428 23.475951058742734 2 15.155576594148595 20.523107778182183 40.73178029600888 23.58953533166034 3 18.566612965108806 25.594932402721092 30.165598595190442 25.672856036979656 4 12.601109993869455 17.246751594647115 38.695825836096205 31.45631257538723 5 13.255489275043422 37.88372930903114 34.99582691779125 13.864954498134194 6 30.772724057137136 37.63517998290571 17.83217019756621 13.759925762390942 7 27.399043675169644 32.525278765392514 22.05184317757224 18.023834381865605 8 25.580767591483834 36.098091120924025 19.964424161416307 18.356717126175834 9 25.901404587288795 16.406768757197057 20.706136585631768 36.985690069882374 10-11 24.790378113812796 26.67531763659393 28.00321284923209 20.531091400361177 12-13 25.320729416454757 24.060537458398016 28.386481997136936 22.23225112801029 14-15 22.649923250591385 25.71636244431041 26.05420384097859 25.579510464119615 16-17 21.988204725696257 28.601706371225404 26.92755617551454 22.4825327275638 18-19 21.682195725724767 27.824041226514513 29.01178338761389 21.48197966014683 20-21 22.705945506558052 26.88418743464792 28.924899721604202 21.484967337189822 22-23 22.544487828294084 26.760956352222053 28.603432790391192 22.09112302909267 24-25 22.22892497767538 26.90229933934584 28.905586180769514 21.963189502209268 26-27 22.190577399175996 27.135358715832798 28.61963777456041 22.054426110430793 28-29 21.92670057084031 27.21683117812912 28.837733137905303 22.018735113125267 30-31 22.4712563945217 26.96328964789497 28.70949910749842 21.85595485008491 32-33 22.313934082477658 27.159553668159216 28.48869155358349 22.03782069577964 34-35 22.449229136985466 27.16246608501421 28.67776962274753 21.710535155252796 36-37 22.38696198348769 27.02056984531908 28.520187465131343 22.072280706061886 38-39 22.30486521661778 27.098700210664962 28.710946666100213 21.88548790661704 40-41 22.401488692947368 26.751120842305316 28.850036598291386 21.997353866455935 42-43 22.194583370281336 26.799252615375025 28.638980510542083 22.367183503801556 44-45 22.217749359798074 27.176186373062176 28.456546732203496 22.14951753493625 46-47 22.280134921285043 27.027432979743683 28.172910726889754 22.51952137208152 48-49 22.542693789897257 27.098321988073415 28.101453751341342 22.257530470687986 50-51 22.40628131540077 27.113720378113875 28.00560425752674 22.47439404895862 52-53 22.218144156881767 27.320002055035612 27.931694134724406 22.53015965335822 54-55 22.22416549055707 27.26431140611417 27.910337244634725 22.60118585869403 56-57 22.452831839046873 27.164832280163694 27.70475096060582 22.677584920183612 58-59 22.166650791646425 27.186433676460904 28.05811001625824 22.58880551563443 60-61 22.33927078551236 27.403667818397082 27.797994539348103 22.459066856742453 62-63 22.27956736915626 27.245115418853977 27.788284002777168 22.687033209212593 64-65 22.00559310963537 27.142671273879003 27.88776836162834 22.963967254857288 66-67 22.17217644695748 27.094255218372933 27.76339853946123 22.970169795208356 68-69 22.02997863143754 27.18237464820757 27.766244709095982 23.021402011258903 70-71 22.090560878131406 26.92722739624908 27.791826022515707 23.1903857031038 72-73 22.176943760893376 27.110077555798284 27.679402973907514 23.033575709400825 74-75 22.188047417070965 27.277936417174963 27.508458083051014 23.02555808270306 76-77 22.0369653132063 27.227713632830785 27.483751061909174 23.251569992053746 78-79 22.009076291367442 27.13288908569367 27.53013328830643 23.327901334632465 80-81 21.930943622050165 27.455585296796453 27.399661003975634 23.213810077177747 82-83 22.21917590011569 27.15943238464967 27.68318069664731 22.938211018587335 84-85 22.299372310899873 27.177082991977297 27.31515758057863 23.208387116544195 86-87 21.838261294561562 27.425797348747388 27.526253169936027 23.209688186755027 88-89 21.996835699871678 27.092991763065065 27.49409539623866 23.4160771408246 90-91 22.01355954321425 27.106694103133243 27.509763668592296 23.369982685060215 92-93 22.061420186311807 27.168152507442088 27.40274826254543 23.367679043700672 94-95 21.71438338611798 27.074511091831567 27.561260338601066 23.649845183449386 96-97 21.76612190647302 27.056359593434077 27.519845905648623 23.657672594444282 98-99 21.582293876076676 27.130541216616994 27.43839000756211 23.848774899744228 100 21.65395570772119 26.994965727444313 27.37923831696959 23.971840247864908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1443.0 1 1012.5 2 804.5 3 983.5 4 794.5 5 761.5 6 1245.5 7 2385.5 8 4096.0 9 5353.5 10 5107.0 11 4537.0 12 4909.0 13 5659.5 14 6396.0 15 7395.5 16 8509.5 17 9442.5 18 10486.0 19 11644.0 20 13062.5 21 14960.5 22 17506.5 23 21198.5 24 25766.0 25 31408.0 26 39337.5 27 49528.5 28 61989.5 29 79686.0 30 101792.0 31 124207.0 32 151851.5 33 183669.5 34 213867.5 35 243154.0 36 277611.0 37 314925.0 38 334506.5 39 340660.0 40 343445.5 41 343662.0 42 348052.0 43 359392.5 44 375459.0 45 398284.0 46 418618.0 47 428512.0 48 433844.5 49 431460.0 50 419549.0 51 405296.0 52 388223.0 53 368183.5 54 348382.0 55 316992.0 56 280295.5 57 241119.0 58 204446.0 59 176436.0 60 144713.5 61 112945.0 62 87560.0 63 67653.5 64 49170.5 65 33443.5 66 23902.0 67 17537.5 68 12848.0 69 9466.0 70 7081.5 71 5352.5 72 4137.0 73 3099.0 74 2202.0 75 1442.5 76 885.5 77 516.0 78 289.0 79 172.0 80 105.5 81 68.5 82 58.0 83 32.5 84 20.0 85 13.0 86 5.0 87 4.0 88 4.5 89 4.0 90 2.0 91 1.5 92 2.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03060695022277743 2 0.0019425314160967046 3 1.256363602450605E-4 4 0.023204069303722322 5 5.315384471906404E-4 6 6.765034782426333E-5 7 1.9328670806932383E-5 8 0.003991370521631538 9 0.010447146571146952 10-11 0.0011693845838194091 12-13 1.01475521736395E-4 14-15 1.159720248415943E-4 16-17 4.203985900507793E-4 18-19 1.4496503105199287E-5 20-21 4.3489509315597865E-5 22-23 2.5610488819185405E-4 24-25 2.8993006210398574E-5 26-27 9.664335403466191E-5 28-29 0.0011693845838194091 30-31 0.002730174751479199 32-33 0.009954265465570176 34-35 0.019038740744828395 36-37 0.021768915496307596 38-39 0.021488649769607075 40-41 0.005107601260731882 42-43 0.0055618250246947925 44-45 0.009451720024589935 46-47 0.01247182483817312 48-49 1.2108059253586652 50-51 0.019077398086442262 52-53 0.019362495980844514 54-55 0.010292517204691494 56-57 0.008287167608472258 58-59 0.008499782987348516 60-61 0.005537664186186128 62-63 0.007842608179912813 64-65 0.020237118334858206 66-67 0.030215544638937046 68-69 0.030490978197935836 70-71 0.02717611115454693 72-73 0.023663125235386967 74-75 0.009731985751290455 76-77 0.01517300658344192 78-79 0.008857363397276764 80-81 0.0028896362856363914 82-83 0.014631803800847814 84-85 0.024663383949645724 86-87 0.023938558794385754 88-89 0.03370436971958834 90-91 0.021363013409362017 92-93 0.01392630731639478 94-95 0.006982482329004324 96-97 0.0040155313601402025 98-99 0.016037964602052143 100 0.03502355150216148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0347323E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.95076016560557 #Duplication Level Percentage of deduplicated Percentage of total 1 72.18721753045546 41.833041301298216 2 15.715359235248574 18.214340279164496 3 5.732961566712085 9.966884423734992 4 2.533798885118065 5.873422859974232 5 1.2758959731162762 3.6969570767161626 6 0.7298944074373817 2.53787614509723 7 0.46608427222351867 1.8906956513610167 8 0.28968514512246385 1.3429979494824438 9 0.20715458070412718 1.080428888123231 >10 0.8027824136564486 7.705984587924037 >50 0.0354997837651243 1.422934079042791 >100 0.021337043006409172 2.3765602597105806 >500 0.0017003577243227285 0.6920195496613076 >1k 5.356493141671706E-4 0.4458338201411052 >5k 2.328909888535647E-5 0.07930372901856872 >10k+ 6.98672966560694E-5 0.8407193995496429 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26901 0.25998028668864404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22573 0.21815304306244235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12819 0.1238871155370331 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.9328670806932383E-5 0.0 0.0 0.0 3 0.0 1.9328670806932383E-5 0.0 0.0 0.0 4 0.0 1.9328670806932383E-5 0.0 0.0 9.664335403466191E-6 5 0.0 1.9328670806932383E-5 0.0 1.9328670806932383E-5 9.664335403466191E-6 6 3.8657341613864766E-5 1.9328670806932383E-5 0.0 1.9328670806932383E-5 2.8993006210398574E-5 7 3.8657341613864766E-5 1.9328670806932383E-5 0.0 1.9328670806932383E-5 2.8993006210398574E-5 8 3.8657341613864766E-5 1.9328670806932383E-5 0.0 2.8993006210398574E-5 2.8993006210398574E-5 9 3.8657341613864766E-5 1.9328670806932383E-5 0.0 2.8993006210398574E-5 2.8993006210398574E-5 10-11 3.8657341613864766E-5 1.9328670806932383E-5 0.0 3.8657341613864766E-5 2.8993006210398574E-5 12-13 3.8657341613864766E-5 2.416083850866548E-5 0.0 7.731468322772953E-5 2.8993006210398574E-5 14-15 3.8657341613864766E-5 8.214685092946262E-5 0.0 1.063076894381281E-4 5.798601242079715E-5 16-17 9.664335403466191E-5 1.063076894381281E-4 0.0 1.063076894381281E-4 5.798601242079715E-5 18-19 1.063076894381281E-4 1.063076894381281E-4 0.0 1.256363602450605E-4 5.798601242079715E-5 20-21 1.111398571398612E-4 1.063076894381281E-4 0.0 1.6429370185892523E-4 6.765034782426333E-5 22-23 1.159720248415943E-4 1.063076894381281E-4 0.0 3.9140558384038077E-4 9.181118633292882E-5 24-25 1.4013286335025978E-4 1.256363602450605E-4 0.0 9.809300434518184E-4 1.063076894381281E-4 26-27 1.4496503105199286E-4 1.4496503105199286E-4 0.0 0.002019846099324434 1.3046852794679358E-4 28-29 1.4496503105199286E-4 1.4496503105199286E-4 0.0 0.005344377478116804 1.3530069564852667E-4 30-31 1.6429370185892523E-4 1.4496503105199286E-4 0.0 0.016028300266648678 1.3530069564852667E-4 32-33 1.7879020496412452E-4 1.4496503105199286E-4 0.0 0.041798250619991276 1.5462936645545906E-4 34-35 1.9811887577105691E-4 1.4496503105199286E-4 0.0 0.08233047330212848 1.6429370185892526E-4 36-37 2.126153788762562E-4 1.8845454036759074E-4 0.0 0.14633736667928507 1.6429370185892526E-4 38-39 2.126153788762562E-4 2.51272720490121E-4 0.0 0.23451959506821232 1.6429370185892526E-4 40-41 2.222797142797224E-4 2.7060139129705333E-4 0.0 0.32609883735145795 1.6429370185892526E-4 42-43 2.319440496831886E-4 2.7060139129705333E-4 0.0 0.4202197998458152 1.6912586956065835E-4 44-45 2.416083850866548E-4 2.7060139129705333E-4 0.0 0.5368441673271435 1.7395803726239143E-4 46-47 2.51272720490121E-4 2.7060139129705333E-4 0.0 0.6747059118575887 1.8845454036759074E-4 48-49 2.7060139129705333E-4 2.8026572670051956E-4 0.0 0.8212462295803465 2.0295104347279003E-4 50-51 3.1892306831438435E-4 2.8993006210398573E-4 0.0 0.9774122253649568 2.0295104347279003E-4 52-53 3.382517391213167E-4 2.8993006210398573E-4 0.0 1.1306595918577202 2.126153788762562E-4 54-55 3.382517391213167E-4 2.9959439750745195E-4 0.0 1.3030471746170482 2.367762173849217E-4 56-57 3.382517391213167E-4 2.9959439750745195E-4 0.0 1.5077522949655675 2.416083850866548E-4 58-59 3.382517391213167E-4 2.9959439750745195E-4 0.0 1.7344969322016912 2.464405527883879E-4 60-61 3.865734161386476E-4 3.237552360161174E-4 0.0 1.9624206183570378 2.51272720490121E-4 62-63 4.155664223490462E-4 3.285874037178505E-4 0.0 2.193755814909808 2.7060139129705333E-4 64-65 4.155664223490462E-4 3.285874037178505E-4 0.0 2.431223032276078 2.7060139129705333E-4 66-67 4.5422376396291096E-4 3.382517391213167E-4 0.0 2.7024671018774615 2.7060139129705333E-4 68-69 4.5422376396291096E-4 3.382517391213167E-4 0.0 3.0014091567451793 2.7060139129705333E-4 70-71 4.5422376396291096E-4 3.430839068230498E-4 0.0 3.3203708824011775 2.8509789440225267E-4 72-73 5.412027825941067E-4 3.4791607452478286E-4 0.0 3.638279195498198 3.285874037178505E-4 74-75 5.412027825941067E-4 3.4791607452478286E-4 0.0 3.952935459731952 3.285874037178505E-4 76-77 5.412027825941067E-4 3.575804099282491E-4 0.0 4.295937219704072 3.334195714195836E-4 78-79 5.412027825941067E-4 3.575804099282491E-4 0.0 4.674532726967159 3.382517391213167E-4 80-81 5.412027825941067E-4 3.575804099282491E-4 0.0 5.077124779037051 3.5758040992824904E-4 82-83 5.412027825941067E-4 3.769090807351815E-4 0.0 5.482389019846003 3.8657341613864766E-4 84-85 5.508671179975729E-4 3.914055838403807E-4 0.0 5.881584058021577 3.8657341613864766E-4 86-87 5.605314534010391E-4 3.9623775154211383E-4 0.0 6.292864347619187 3.8657341613864766E-4 88 5.605314534010391E-4 3.9623775154211383E-4 0.0 6.619441569573116 3.8657341613864766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17220 0.0 49.982925 1 GTATCAA 30055 0.0 39.974102 1 ATCAACG 36505 0.0 32.247704 3 CAACGCA 36610 0.0 32.12415 5 TATCAAC 38595 0.0 31.033625 2 TCAACGC 38285 0.0 30.809824 4 AACGCAG 39170 0.0 30.38393 6 ACGCAGA 43950 0.0 26.69512 7 CGCAGAG 45150 0.0 25.954445 8 GTGGTAT 8500 0.0 24.238237 1 GCAGAGT 50070 0.0 23.17879 9 GTACATG 39325 0.0 22.877514 1 TGGTATC 8785 0.0 22.37613 2 TACATGG 40200 0.0 22.103785 2 GTACTAG 4195 0.0 22.038889 1 ACATGGG 40470 0.0 21.193052 3 GAGTACT 27490 0.0 21.029522 12-13 CAGAGTA 49360 0.0 19.932024 10-11 AGAGTAC 46710 0.0 19.67705 10-11 GTACTTT 29470 0.0 19.46541 14-15 >>END_MODULE