FastQCFastQC Report
Mon 6 Jun 2016
ERR1211317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951896
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126100.42718307149032353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100080.33903633461341454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56890.1927235918880611No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.1591180719103925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56850.052.530751
TACCTGG31700.052.5169682
ACCTGGG37050.043.5026253
GTACCTG40800.041.8424261
GTATCAA101800.039.915021
ATCAACG123050.032.2307433
CAACGCA123150.032.166965
TCAACGC124350.031.8560054
TATCAAC127450.031.290442
AACGCAG131400.030.8268226
TAACGCA4150.029.4397934
GTACATG112000.028.133721
ACGCAGA146750.027.2825987
CCTGGGG34000.027.0886064
CGCAGAG150300.026.5770178
TACATGG117650.026.2620772
ACATGGG116950.025.9160613
GCAGAGT160300.023.834359
TATAACG5350.023.7337112
CATGGGG72850.023.4790944
Produced by FastQC (version 0.11.5)