##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2951896 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993529582343008 31.0 31.0 34.0 27.0 34.0 2 30.980276405401817 31.0 31.0 34.0 27.0 34.0 3 31.014937179358622 31.0 31.0 34.0 27.0 34.0 4 34.624315016518196 37.0 35.0 37.0 31.0 37.0 5 34.67852729228943 37.0 35.0 37.0 31.0 37.0 6 34.746166531612225 37.0 35.0 37.0 31.0 37.0 7 34.68336587738863 37.0 35.0 37.0 30.0 37.0 8 34.6798149392797 37.0 35.0 37.0 30.0 37.0 9 36.17369649879264 39.0 35.0 39.0 30.0 39.0 10-11 36.08687789136203 39.0 35.0 39.0 30.0 39.0 12-13 36.00193926208783 39.0 35.0 39.0 30.0 39.0 14-15 37.04954798543038 40.0 36.0 41.0 30.0 41.0 16-17 37.03646537682899 40.0 36.0 41.0 30.0 41.0 18-19 37.059073558147034 40.0 36.0 41.0 30.0 41.0 20-21 37.02712087417714 40.0 36.0 41.0 30.0 41.0 22-23 36.8867500752059 40.0 36.0 41.0 30.0 41.0 24-25 36.76618146438763 39.0 36.0 41.0 30.0 41.0 26-27 36.61060027182529 39.0 36.0 41.0 29.0 41.0 28-29 36.45832627572245 39.0 35.0 41.0 29.0 41.0 30-31 36.25530862198397 39.0 35.0 40.5 28.0 41.0 32-33 36.09376278839092 39.0 35.0 40.0 27.5 41.0 34-35 35.91784636044088 38.5 35.0 40.0 27.0 41.0 36-37 35.79257602571364 38.0 35.0 40.0 27.0 41.0 38-39 35.62976439549361 38.0 34.5 40.0 26.0 41.0 40-41 35.46502519058937 38.0 34.0 40.0 25.5 41.0 42-43 35.316662748281104 38.0 34.0 40.0 25.0 41.0 44-45 35.18063949407431 38.0 34.0 40.0 24.5 41.0 46-47 35.0091353150653 38.0 33.0 40.0 24.0 41.0 48-49 34.761278683259846 38.0 33.0 40.0 23.0 41.0 50-51 34.12223431990829 37.5 32.5 39.5 22.5 40.5 52-53 34.388265372492796 38.0 33.0 39.5 23.0 40.5 54-55 34.75506437218655 38.0 33.0 40.0 23.0 41.0 56-57 34.674571699002946 38.0 33.0 40.0 23.0 41.0 58-59 34.471356375698875 38.0 33.0 40.0 23.0 41.0 60-61 34.21575810936429 37.0 33.0 40.0 22.0 41.0 62-63 33.94380899598089 37.0 32.0 40.0 22.0 41.0 64-65 33.67939487027998 36.0 32.0 40.0 22.0 41.0 66-67 33.46772887662709 36.0 32.0 39.0 21.5 41.0 68-69 33.12812782022131 35.5 31.5 39.0 20.5 41.0 70-71 32.778807247951825 35.0 31.0 39.0 20.0 40.0 72-73 32.407091408369396 35.0 31.0 38.0 20.0 40.0 74-75 32.02757803797966 35.0 30.5 37.0 19.5 39.5 76-77 31.664893004360586 35.0 30.0 37.0 18.5 39.0 78-79 31.283898043833524 34.5 30.0 36.0 18.0 39.0 80-81 30.93802457810167 34.0 29.0 36.0 18.0 37.5 82-83 30.61552270134178 34.0 29.0 35.0 17.5 37.0 84-85 30.29790666744357 34.0 29.0 35.0 16.5 37.0 86-87 30.0332650608287 34.0 29.0 35.0 15.0 36.0 88-89 29.764802350760327 34.0 29.0 35.0 14.0 36.0 90-91 29.497237707561514 34.0 27.5 35.0 12.0 35.5 92-93 29.2542020450585 34.0 27.0 35.0 11.0 35.0 94-95 29.021791587508503 34.0 27.0 35.0 10.0 35.0 96-97 28.812541329369328 33.0 26.0 35.0 10.0 35.0 98-99 28.572081977142826 33.0 25.5 35.0 10.0 35.0 100 26.9158523877535 31.0 23.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24060456872609137 1101 2 -0.2640059993285817 1101 3 -0.35732561925401285 1101 4 -0.35623141302302486 1101 5 -0.2976092136490749 1101 6 -0.4029450377897348 1101 7 -0.4550765248567359 1101 8 -0.4828179608294434 1101 9 -0.4757416951040554 1101 10-11 -0.5333697230415666 1101 12-13 -0.4637096659851281 1101 14-15 -0.6320147567211833 1101 16-17 -0.6064757940734538 1101 18-19 -0.6941557176285116 1101 20-21 -0.5723493190433757 1101 22-23 -0.6385027589693877 1101 24-25 -0.5387811104190803 1101 26-27 -0.7161157101636775 1101 28-29 -0.7996069209845942 1101 30-31 -0.8566843146394802 1101 32-33 -0.7192237134997939 1101 34-35 -0.6364303951268084 1101 36-37 -0.6389484681921047 1101 38-39 -0.7684710674144455 1101 40-41 -0.8123660815056297 1101 42-43 -0.7516647370340621 1101 44-45 -0.790038485929287 1101 46-47 -0.6507226787686093 1101 48-49 -0.6915177487529007 1101 50-51 -0.5721522332315843 1101 52-53 -0.46864868462088083 1101 54-55 -0.3912081159472294 1101 56-57 -0.4149910216843935 1101 58-59 -0.5881703710317012 1101 60-61 -0.4275486952450507 1101 62-63 -0.30364215498871516 1101 64-65 -0.4191904627355356 1101 66-67 -0.7259934091221254 1101 68-69 -0.6470755097492571 1101 70-71 -0.570426638936226 1101 72-73 -0.6271034056623108 1101 74-75 -0.7786112335021897 1101 76-77 -0.714350264170978 1101 78-79 -0.5955238525180704 1101 80-81 -0.6781683514222614 1101 82-83 -0.8793886644072231 1101 84-85 -0.7854277408613193 1101 86-87 -0.796974809488912 1101 88-89 -0.7820923519304621 1101 90-91 -0.890549391258272 1101 92-93 -0.9277912256863026 1101 94-95 -1.017445759713329 1101 96-97 -1.0411335542421476 1101 98-99 -1.0378327821241342 1101 100 -1.109026262461228 1102 1 -0.19695143300134532 1102 2 -0.23648295357271465 1102 3 -0.3584019457820382 1102 4 -0.20362884504218925 1102 5 -0.12644734652152323 1102 6 -0.16493387259434655 1102 7 -0.21716309214089335 1102 8 -0.25645684566377014 1102 9 -0.3451491020413684 1102 10-11 -0.38301020713465306 1102 12-13 -0.31038954698560417 1102 14-15 -0.514344639801827 1102 16-17 -0.4630139629785859 1102 18-19 -0.45502577644428044 1102 20-21 -0.4631501260062123 1102 22-23 -0.4861228038586347 1102 24-25 -0.46881586679654674 1102 26-27 -0.42772324021853336 1102 28-29 -0.587056053908519 1102 30-31 -0.5624608259848358 1102 32-33 -0.6073975193619034 1102 34-35 -0.5763352152560373 1102 36-37 -0.5823705293602259 1102 38-39 -0.6136849612216935 1102 40-41 -0.706633077331901 1102 42-43 -0.6366317752392874 1102 44-45 -0.7044857579873138 1102 46-47 -0.8504044488406137 1102 48-49 -0.8038941640581143 1102 50-51 -0.6891265879751032 1102 52-53 -0.6312588892738162 1102 54-55 -0.465230526724028 1102 56-57 -0.5529088262716115 1102 58-59 -0.6903197099271807 1102 60-61 -0.7764845323914287 1102 62-63 -0.6471101208063175 1102 64-65 -0.7840743225620557 1102 66-67 -0.6247770416818454 1102 68-69 -0.5367253158003535 1102 70-71 -0.570980456320342 1102 72-73 -0.6367994407275432 1102 74-75 -0.68139153215742 1102 76-77 -0.6405925631548008 1102 78-79 -0.6216561495768609 1102 80-81 -0.8457319568622808 1102 82-83 -0.7519111002165175 1102 84-85 -0.8682464978818736 1102 86-87 -0.7409846053013638 1102 88-89 -0.784980466575032 1102 90-91 -0.9716740481192545 1102 92-93 -1.0054239560539564 1102 94-95 -0.8682622066934584 1102 96-97 -0.920000351077757 1102 98-99 -0.9772617734135309 1102 100 -0.9389229523199667 1103 1 -0.5476297788101263 1103 2 -0.4846634494053852 1103 3 -0.5686660005678306 1103 4 -0.40827586951530037 1103 5 -0.3789559200735795 1103 6 -0.47680136741836066 1103 7 -0.602534011077033 1103 8 -0.600691277898207 1103 9 -0.7738250058477121 1103 10-11 -0.7876294140147309 1103 12-13 -0.8930827571570816 1103 14-15 -0.828188245471523 1103 16-17 -0.8962891129493968 1103 18-19 -0.9851556272697835 1103 20-21 -0.9607476202993581 1103 22-23 -0.8783636065342222 1103 24-25 -1.0876774689624966 1103 26-27 -0.9879704133352689 1103 28-29 -0.8769453144064769 1103 30-31 -0.9784567913721105 1103 32-33 -0.9342185630525179 1103 34-35 -1.14416618621744 1103 36-37 -1.2097954908721391 1103 38-39 -1.161636333520896 1103 40-41 -1.1809461845145677 1103 42-43 -1.21392009078847 1103 44-45 -1.2103529342892756 1103 46-47 -1.3667877370499824 1103 48-49 -1.3580344199374963 1103 50-51 -1.4070925061149424 1103 52-53 -1.1275069117827101 1103 54-55 -1.1352493195254354 1103 56-57 -1.041315048069137 1103 58-59 -1.2400966383128065 1103 60-61 -1.1332094885946447 1103 62-63 -1.1976712504978835 1103 64-65 -1.0929249888040289 1103 66-67 -1.0131140380188484 1103 68-69 -1.0209022017163676 1103 70-71 -1.0984204945429887 1103 72-73 -1.3487229954337039 1103 74-75 -1.029143084952448 1103 76-77 -1.1904102623638018 1103 78-79 -1.0522026272537737 1103 80-81 -1.0639361295012364 1103 82-83 -1.2164935708859446 1103 84-85 -1.174402892212182 1103 86-87 -1.338659818976577 1103 88-89 -1.3714003848210368 1103 90-91 -1.1725217414886835 1103 92-93 -1.1304640367462113 1103 94-95 -1.3396194291811163 1103 96-97 -1.324417190277206 1103 98-99 -1.4732747266438793 1103 100 -1.4868594602564755 1104 1 -0.3795158409975592 1104 2 -0.351623223269268 1104 3 -0.4485120540577192 1104 4 -0.303846210893866 1104 5 -0.18215052759695283 1104 6 -0.19092487252816426 1104 7 -0.19429004499745162 1104 8 -0.267404041542477 1104 9 -0.3545195046517975 1104 10-11 -0.41082489129239974 1104 12-13 -0.3882047401968691 1104 14-15 -0.48818628852436063 1104 16-17 -0.5868342314534161 1104 18-19 -0.7243354489701588 1104 20-21 -0.6599565787685933 1104 22-23 -0.4960728911613117 1104 24-25 -0.6429847096669974 1104 26-27 -0.5871708917001328 1104 28-29 -0.6090853448478768 1104 30-31 -0.6836085634964846 1104 32-33 -0.6561731183906332 1104 34-35 -0.5091337878699704 1104 36-37 -0.5958668107242886 1104 38-39 -0.6997652745950376 1104 40-41 -0.7679657539861964 1104 42-43 -0.6606998255134116 1104 44-45 -0.7642690029998604 1104 46-47 -0.8019858235905346 1104 48-49 -0.7767819737535433 1104 50-51 -0.7105284704550101 1104 52-53 -0.5137597928438069 1104 54-55 -0.5002171386165415 1104 56-57 -0.5575596664644422 1104 58-59 -0.563156868797698 1104 60-61 -0.3695204257548994 1104 62-63 -0.43588281822999875 1104 64-65 -0.497248754840129 1104 66-67 -0.6134493282993461 1104 68-69 -0.6172264026296119 1104 70-71 -0.5755403031970445 1104 72-73 -0.5171056873232445 1104 74-75 -0.6141143830607341 1104 76-77 -0.638555076453823 1104 78-79 -0.6362952560861288 1104 80-81 -0.6346745508971914 1104 82-83 -0.6320067259196485 1104 84-85 -0.662098913086286 1104 86-87 -0.7379551005595282 1104 88-89 -0.7673105826901399 1104 90-91 -0.8598318088946435 1104 92-93 -0.7545649282443634 1104 94-95 -0.809871223049349 1104 96-97 -0.87605885150791 1104 98-99 -1.0453954050522256 1104 100 -1.033671810767455 1105 1 -0.39785755628987474 1105 2 -0.4265924589363479 1105 3 -0.3514856633183996 1105 4 -0.3200591429448423 1105 5 -0.2287777107500446 1105 6 -0.40585753393303037 1105 7 -0.17320926097421108 1105 8 -0.23513907218678298 1105 9 -0.30949598201136297 1105 10-11 -0.39224740661169477 1105 12-13 -0.4821879911395186 1105 14-15 -0.5407360701590349 1105 16-17 -0.6310616394590554 1105 18-19 -0.6178322719419285 1105 20-21 -0.5751929111456988 1105 22-23 -0.6445946742523319 1105 24-25 -0.6768253329898144 1105 26-27 -0.5901179376097119 1105 28-29 -0.7078918659147959 1105 30-31 -0.6536697806609553 1105 32-33 -0.7225236968972624 1105 34-35 -0.7405017190035892 1105 36-37 -0.7551236027402481 1105 38-39 -0.7203055751567078 1105 40-41 -0.674264783302192 1105 42-43 -0.6069869863412762 1105 44-45 -0.8018254947742207 1105 46-47 -0.7714218232041574 1105 48-49 -0.8586985473984754 1105 50-51 -0.8839768649654971 1105 52-53 -0.5800989401073693 1105 54-55 -0.5724312303671297 1105 56-57 -0.6783475391729823 1105 58-59 -0.6579946051600842 1105 60-61 -0.7431676095824358 1105 62-63 -0.761541572190211 1105 64-65 -0.6912337347582849 1105 66-67 -0.6462199872704915 1105 68-69 -0.6469905732271997 1105 70-71 -0.6796061963114965 1105 72-73 -0.6133204598043562 1105 74-75 -0.637301016393895 1105 76-77 -0.7251497434359884 1105 78-79 -0.735933542977179 1105 80-81 -0.69814838563914 1105 82-83 -0.69819412343141 1105 84-85 -0.6169833689391133 1105 86-87 -0.7798569650524314 1105 88-89 -0.8503935003548975 1105 90-91 -0.936889318335421 1105 92-93 -0.7229301061630871 1105 94-95 -0.6888778023013025 1105 96-97 -0.8485932895565718 1105 98-99 -0.9334398823043379 1105 100 -0.9904104142467816 1106 1 -0.5447711439723797 1106 2 -0.5042750371214098 1106 3 -0.5515311006548629 1106 4 -0.3278700507909491 1106 5 -0.3654713993723462 1106 6 -0.3818278500740888 1106 7 -0.31813167364074957 1106 8 -0.38780852270735267 1106 9 -0.37318444884162005 1106 10-11 -0.5060717862638882 1106 12-13 -0.551557455652663 1106 14-15 -0.6770744819504415 1106 16-17 -0.6235232295725481 1106 18-19 -0.7133187504825216 1106 20-21 -0.7300108305442947 1106 22-23 -0.7125722351553847 1106 24-25 -0.6697309316008173 1106 26-27 -0.706997641981296 1106 28-29 -0.7807851751549535 1106 30-31 -0.6533636669160572 1106 32-33 -0.8682118491397972 1106 34-35 -0.8738754986630397 1106 36-37 -1.0881175100192308 1106 38-39 -1.022864730885054 1106 40-41 -1.063949541470933 1106 42-43 -0.8890636939216279 1106 44-45 -1.0263693460759171 1106 46-47 -0.9412912805039255 1106 48-49 -0.9030101752526818 1106 50-51 -0.8655115040012973 1106 52-53 -0.6693524917901712 1106 54-55 -0.8200788607413969 1106 56-57 -0.7681194742782651 1106 58-59 -0.8538139079883038 1106 60-61 -0.9580768266525794 1106 62-63 -0.736353334297462 1106 64-65 -0.7679243671454401 1106 66-67 -0.6932885997495433 1106 68-69 -0.8150511511133587 1106 70-71 -0.9616889267200364 1106 72-73 -0.8965340112238351 1106 74-75 -0.9067454721214148 1106 76-77 -0.7998316225527873 1106 78-79 -0.8564726757431416 1106 80-81 -0.855547717217263 1106 82-83 -0.7737781661862044 1106 84-85 -0.6203494295738672 1106 86-87 -0.6935632132324514 1106 88-89 -0.7409530393031964 1106 90-91 -0.7909536698736197 1106 92-93 -0.6275024552467734 1106 94-95 -0.8166822166982151 1106 96-97 -0.86327085184627 1106 98-99 -0.9302380487873556 1106 100 -0.730460106781404 1107 1 -0.6265012227040927 1107 2 -0.5238350788723309 1107 3 -0.5378584346383484 1107 4 -0.4472609045017677 1107 5 -0.3101695673487228 1107 6 -0.31773216061515086 1107 7 -0.3528850334309155 1107 8 -0.4319195667110449 1107 9 -0.6025944697290768 1107 10-11 -0.586857975228277 1107 12-13 -0.6098695865831658 1107 14-15 -0.8148615178551921 1107 16-17 -0.8144087990075874 1107 18-19 -0.8831542312161886 1107 20-21 -0.7872446494292049 1107 22-23 -0.7556702445988108 1107 24-25 -0.8531115905553577 1107 26-27 -0.865839967449368 1107 28-29 -0.8106847971856439 1107 30-31 -0.7618417786884564 1107 32-33 -0.8509162493490621 1107 34-35 -0.9882485874296236 1107 36-37 -0.9468248931228516 1107 38-39 -0.9185057684596885 1107 40-41 -0.9186696637525245 1107 42-43 -0.8881649449689704 1107 44-45 -0.8899412687292312 1107 46-47 -1.0325007969611164 1107 48-49 -1.050394601764964 1107 50-51 -0.8236591676740872 1107 52-53 -0.8319500396237558 1107 54-55 -0.8815005070222455 1107 56-57 -0.8798715286520746 1107 58-59 -0.8211826999094143 1107 60-61 -0.7182797509505718 1107 62-63 -0.7095816306671097 1107 64-65 -0.8178459074753945 1107 66-67 -0.7291379411803973 1107 68-69 -0.6204827046361601 1107 70-71 -0.7247209865667585 1107 72-73 -0.8437379944973245 1107 74-75 -0.7829133490108795 1107 76-77 -0.6779714929339704 1107 78-79 -0.7656114836815817 1107 80-81 -0.8673443389773752 1107 82-83 -0.7754965843187058 1107 84-85 -0.676179149086046 1107 86-87 -0.8701049387477617 1107 88-89 -0.8943111225161857 1107 90-91 -0.9989604768684295 1107 92-93 -0.9555848294322509 1107 94-95 -0.974029494387036 1107 96-97 -1.0634658308274574 1107 98-99 -1.1714871651409062 1107 100 -0.8993170253347067 1108 1 -0.39177402892620705 1108 2 -0.49613824690835173 1108 3 -0.3589515084871415 1108 4 -0.31612171381185306 1108 5 -0.36024178913409344 1108 6 -0.3584898573322022 1108 7 -0.3072913757780569 1108 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1.1536505586859533 2207 60-61 0.9565892068907047 2207 62-63 1.141230569854251 2207 64-65 1.102177539225984 2207 66-67 1.187471885159738 2207 68-69 1.243979923022522 2207 70-71 1.1970890761608501 2207 72-73 1.1754934005248572 2207 74-75 1.1162743171023735 2207 76-77 1.111784221159244 2207 78-79 1.1444868044723648 2207 80-81 1.142140180342306 2207 82-83 1.1391034790351888 2207 84-85 1.1662522640311543 2207 86-87 1.1347164866038462 2207 88-89 1.1881686990345308 2207 90-91 1.153140022590268 2207 92-93 1.4473668879244634 2207 94-95 1.422435224014091 2207 96-97 1.441068592218425 2207 98-99 1.4273458130670846 2207 100 1.5261123605219709 2208 1 0.7440470589152568 2208 2 0.7518392089395078 2208 3 0.8271701066906054 2208 4 0.754346183189277 2208 5 0.6993478247253151 2208 6 0.7036376062293499 2208 7 0.6756325400094241 2208 8 0.6917554396237549 2208 9 0.8046832003473909 2208 10-11 0.9026086234332524 2208 12-13 0.9027643880116685 2208 14-15 1.1347335645146117 2208 16-17 1.082627206976639 2208 18-19 1.0174407088374764 2208 20-21 1.002775149002538 2208 22-23 1.0765170568962006 2208 24-25 1.22582125965517 2208 26-27 1.1830011539324161 2208 28-29 1.0745672260466037 2208 30-31 1.1825289344716268 2208 32-33 1.143805362051502 2208 34-35 1.1178191350955515 2208 36-37 1.061186247454259 2208 38-39 1.1769440525170225 2208 40-41 1.1399716379542184 2208 42-43 1.1579930506817178 2208 44-45 1.1901377037135035 2208 46-47 1.1760335181511223 2208 48-49 1.1190174177878873 2208 50-51 0.9894145442780413 2208 52-53 0.873686916233936 2208 54-55 0.9301964155647227 2208 56-57 0.9422443023700637 2208 58-59 0.9934153242392014 2208 60-61 1.0744289765001298 2208 62-63 1.1063922608289758 2208 64-65 1.0225000574280614 2208 66-67 0.9991182782086199 2208 68-69 0.9547308888985242 2208 70-71 0.9979240500709778 2208 72-73 0.957960025833053 2208 74-75 0.8610907916078787 2208 76-77 0.9352281449408686 2208 78-79 0.9634797583218173 2208 80-81 0.9985857729490633 2208 82-83 1.023756082373417 2208 84-85 1.0593844401965065 2208 86-87 1.036996734421482 2208 88-89 1.0465514760203867 2208 90-91 1.0947896316042396 2208 92-93 1.1595316289958113 2208 94-95 1.2037796115031192 2208 96-97 1.1233694486502124 2208 98-99 1.1432565378036514 2208 100 1.044457576266872 2209 1 0.5172262814805002 2209 2 0.41677925090411705 2209 3 0.47565971316272027 2209 4 0.4261320915123008 2209 5 0.46636464452650017 2209 6 0.4825344363872759 2209 7 0.5900536837502415 2209 8 0.48921048613005524 2209 9 0.7082011145392357 2209 10-11 0.6632383879588701 2209 12-13 0.6599590604443435 2209 14-15 0.8580320151771872 2209 16-17 0.7924729028449704 2209 18-19 0.8666810994931993 2209 20-21 0.866218167822943 2209 22-23 0.8540720293348159 2209 24-25 0.8916747676476788 2209 26-27 0.9269446884676213 2209 28-29 0.8927443776172268 2209 30-31 0.9398936548396009 2209 32-33 0.9860149382174157 2209 34-35 1.0394348186263471 2209 36-37 1.0169201966269839 2209 38-39 1.0404424717698006 2209 40-41 1.1844897983309153 2209 42-43 1.1447666727540238 2209 44-45 1.2227286329339506 2209 46-47 1.2351105849719133 2209 48-49 1.3336814479893917 2209 50-51 1.2599287982389242 2209 52-53 1.1229067119005194 2209 54-55 1.0758013529149224 2209 56-57 1.083786393964978 2209 58-59 1.0678464020336094 2209 60-61 1.1886339833904813 2209 62-63 1.1878703797362604 2209 64-65 1.1261401178565364 2209 66-67 1.1692427925594941 2209 68-69 1.2587208430713517 2209 70-71 1.3186191579253972 2209 72-73 1.2867739719921474 2209 74-75 1.2749971490484668 2209 76-77 1.190964985724353 2209 78-79 1.1837037723798147 2209 80-81 1.168662668572349 2209 82-83 1.1751640049978072 2209 84-85 1.1312315056448874 2209 86-87 1.113541871492675 2209 88-89 1.3624175496975681 2209 90-91 1.360346143759262 2209 92-93 1.3171180925788484 2209 94-95 1.3311955213400424 2209 96-97 1.357676049233696 2209 98-99 1.3345351302630348 2209 100 1.6187052092137613 2210 1 0.851103439778516 2210 2 0.8468276578809046 2210 3 0.9107895358636107 2210 4 0.8162817896929582 2210 5 0.6941542348317569 2210 6 0.7132571523750997 2210 7 0.8347425236332384 2210 8 0.8532048781545782 2210 9 0.9705249654661188 2210 10-11 0.9778714465704681 2210 12-13 0.971576164242812 2210 14-15 1.2023814619295123 2210 16-17 1.229460169896278 2210 18-19 1.166513334290741 2210 20-21 1.1781384955927834 2210 22-23 1.234307076196707 2210 24-25 1.240037103613524 2210 26-27 1.2739065229814273 2210 28-29 1.2511189426216376 2210 30-31 1.362657092735752 2210 32-33 1.3988520049337154 2210 34-35 1.4300581396574898 2210 36-37 1.3879130576074914 2210 38-39 1.448104056611065 2210 40-41 1.4114471029208886 2210 42-43 1.4971427319309853 2210 44-45 1.509672883266667 2210 46-47 1.5140960839482958 2210 48-49 1.5977228950366822 2210 50-51 1.527079037201183 2210 52-53 1.381334740540872 2210 54-55 1.4080114377895399 2210 56-57 1.3961201211792869 2210 58-59 1.338559127608022 2210 60-61 1.3879276313135591 2210 62-63 1.3282424041205942 2210 64-65 1.2871768471110556 2210 66-67 1.3136536039777127 2210 68-69 1.2745998063157629 2210 70-71 1.3537134698861593 2210 72-73 1.3212316804125308 2210 74-75 1.349252784238601 2210 76-77 1.3390498638553545 2210 78-79 1.3246725878925218 2210 80-81 1.3324095388866048 2210 82-83 1.4070348284255871 2210 84-85 1.3413326212703183 2210 86-87 1.4796775304210144 2210 88-89 1.4358144746167163 2210 90-91 1.369511821334033 2210 92-93 1.3712614365756473 2210 94-95 1.3733231984723524 2210 96-97 1.489646206165709 2210 98-99 1.4653764494892272 2210 100 1.5062243033694571 2211 1 0.7323936322854152 2211 2 0.7354355806278647 2211 3 0.7477414404235674 2211 4 0.6391372044274917 2211 5 0.6334545984327278 2211 6 0.6524892104400166 2211 7 0.7235464989111549 2211 8 0.6967812030734137 2211 9 0.8442952920117648 2211 10-11 0.7904396652037988 2211 12-13 0.8195196114302874 2211 14-15 1.0040049785001202 2211 16-17 0.9958620150147723 2211 18-19 0.9623049832871899 2211 20-21 0.9635377092969932 2211 22-23 1.0839426061989954 2211 24-25 1.0211235092475945 2211 26-27 1.0457150206767736 2211 28-29 1.1109538969803268 2211 30-31 1.1900103314262296 2211 32-33 1.103681362757392 2211 34-35 1.088530980820181 2211 36-37 1.095238609092135 2211 38-39 1.0471920125896261 2211 40-41 1.2112323663056372 2211 42-43 1.1317389645243665 2211 44-45 1.1745295980658526 2211 46-47 1.2251837409814996 2211 48-49 1.1780236132007431 2211 50-51 1.100996576950486 2211 52-53 1.0408396383780385 2211 54-55 1.0349857806311888 2211 56-57 1.0241772718825004 2211 58-59 0.9873103355557902 2211 60-61 1.0430604534271737 2211 62-63 1.046191030710041 2211 64-65 1.0572132245886507 2211 66-67 1.070811607022783 2211 68-69 1.073371697110332 2211 70-71 1.039789655760643 2211 72-73 1.0512414482779988 2211 74-75 1.1567441461084655 2211 76-77 1.1844344605224713 2211 78-79 1.187588156688193 2211 80-81 1.152895898636043 2211 82-83 1.2638806769089612 2211 84-85 1.2401109428659929 2211 86-87 1.1662288134527365 2211 88-89 1.2125934601491473 2211 90-91 1.2490044311269273 2211 92-93 1.357578169172509 2211 94-95 1.4194148356500342 2211 96-97 1.4106074023642385 2211 98-99 1.5139465521734792 2211 100 1.5778430924322286 2212 1 0.5884771071097106 2212 2 0.5123875954729975 2212 3 0.5386611662701277 2212 4 0.5009779845443063 2212 5 0.2597861148698968 2212 6 0.4567155904439204 2212 7 0.5197078525749035 2212 8 0.5658342445409872 2212 9 0.7094007563996314 2212 10-11 0.6796552725217566 2212 12-13 0.7445684932711529 2212 14-15 0.8625907884442086 2212 16-17 0.8727578209320725 2212 18-19 0.847133512846689 2212 20-21 0.868175983674611 2212 22-23 0.8981351944250164 2212 24-25 1.0169446619042404 2212 26-27 0.9966791241775823 2212 28-29 0.9205702731235021 2212 30-31 1.0028354319920538 2212 32-33 0.9319602653133572 2212 34-35 0.8060852697606435 2212 36-37 0.9031431867571769 2212 38-39 0.9988501344475864 2212 40-41 0.998831821651919 2212 42-43 1.0825238968785484 2212 44-45 1.1661732733160761 2212 46-47 1.1500961272871137 2212 48-49 1.103137214183775 2212 50-51 1.049237743970707 2212 52-53 0.8791899021946818 2212 54-55 0.8761545859216398 2212 56-57 0.9231234582686483 2212 58-59 0.9529064449348823 2212 60-61 0.9209994870819571 2212 62-63 0.8583479341543949 2212 64-65 0.9451154359989857 2212 66-67 1.025258007202119 2212 68-69 0.9744455201061726 2212 70-71 0.9988237671904798 2212 72-73 0.9169597726375471 2212 74-75 0.8785897002479643 2212 76-77 0.9047086139637095 2212 78-79 1.0515753789127231 2212 80-81 1.1037870633730371 2212 82-83 1.1072679912928827 2212 84-85 1.0334271865819247 2212 86-87 1.0266351450522002 2212 88-89 1.0584605264499451 2212 90-91 1.105537867966607 2212 92-93 1.12741879984166 2212 94-95 1.1721352813361854 2212 96-97 1.1170079553206023 2212 98-99 1.1043107478336864 2212 100 1.119814058071718 2213 1 0.3858129655931606 2213 2 0.49205829859483075 2213 3 0.49613101451779684 2213 4 0.34145870932186995 2213 5 0.3756306478117466 2213 6 0.3942625720243811 2213 7 0.4675079709920098 2213 8 0.4525016141402318 2213 9 0.5075422567281223 2213 10-11 0.5547822464780978 2213 12-13 0.5664561916806363 2213 14-15 0.7636669647072551 2213 16-17 0.6770739621566335 2213 18-19 0.643405117885095 2213 20-21 0.5940706005628371 2213 22-23 0.7079460514890812 2213 24-25 0.6461466036203163 2213 26-27 0.6233086976326874 2213 28-29 0.8011294695093483 2213 30-31 0.7405243113282722 2213 32-33 0.8238859626359982 2213 34-35 0.7351110457576482 2213 36-37 0.7163994949011965 2213 38-39 0.7150324805661867 2213 40-41 0.8332875085116811 2213 42-43 0.8205516437903739 2213 44-45 0.8936316042837689 2213 46-47 0.9013325721785534 2213 48-49 0.8840746153338799 2213 50-51 0.8941451596390735 2213 52-53 0.8759707818877729 2213 54-55 0.812843449036329 2213 56-57 0.8371459494076419 2213 58-59 0.7648254103262673 2213 60-61 0.9273216221585656 2213 62-63 0.8213573101935836 2213 64-65 0.7678870045389701 2213 66-67 0.7586169975746486 2213 68-69 0.8850805115493188 2213 70-71 0.7627406156733656 2213 72-73 0.7640197436010325 2213 74-75 0.8994439661024671 2213 76-77 0.8873068149970216 2213 78-79 0.90709081774542 2213 80-81 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40-41 1.1150654155358666 2311 42-43 1.07561128398563 2311 44-45 1.0248960885325715 2311 46-47 1.0291382916139113 2311 48-49 1.0893349744221865 2311 50-51 1.0807373598120122 2311 52-53 0.9967286196761265 2311 54-55 0.8908052343349482 2311 56-57 0.922426892837855 2311 58-59 0.9934750844628013 2311 60-61 0.9312250930049899 2311 62-63 0.8767153337916156 2311 64-65 0.9242220712556559 2311 66-67 1.023298184063286 2311 68-69 0.9346376629497115 2311 70-71 0.967869254542876 2311 72-73 0.9325343491634399 2311 74-75 0.998782943711447 2311 76-77 1.0409991437640507 2311 78-79 0.9880090316375032 2311 80-81 1.0976268512073766 2311 82-83 1.1293753821674777 2311 84-85 1.1842005625854455 2311 86-87 1.1577261769045641 2311 88-89 1.1497481882887008 2311 90-91 1.2075249949464002 2311 92-93 1.1091997299078464 2311 94-95 1.190388724477522 2311 96-97 1.2059488837781878 2311 98-99 1.1442448799631109 2311 100 1.263391453877933 2312 1 0.5048853933685322 2312 2 0.6146605646871244 2312 3 0.6865889147634583 2312 4 0.4290064352920595 2312 5 0.5554835616857119 2312 6 0.5802842104882231 2312 7 0.6183250638178421 2312 8 0.6593454308186963 2312 9 0.6816882342932118 2312 10-11 0.6729554819961834 2312 12-13 0.7680871485140202 2312 14-15 0.9874222216270141 2312 16-17 0.8709523917670552 2312 18-19 0.8361009928006737 2312 20-21 0.8712560733021846 2312 22-23 0.8638122088993541 2312 24-25 0.9608924821683118 2312 26-27 0.9494729893241285 2312 28-29 0.9309277961742737 2312 30-31 1.031442856315131 2312 32-33 1.0083920745199393 2312 34-35 1.0579975823926304 2312 36-37 1.0227147943875465 2312 38-39 0.9987179681042946 2312 40-41 1.05082981275784 2312 42-43 1.0537606115640727 2312 44-45 1.0612094591645302 2312 46-47 1.146009058040363 2312 48-49 1.1750153584464087 2312 50-51 1.1986252459023348 2312 52-53 1.0458960885582442 2312 54-55 0.8953834796562887 2312 56-57 0.9941112503738694 2312 58-59 0.9803029525497919 2312 60-61 0.9911661222702222 2312 62-63 0.9285180891998124 2312 64-65 0.9954603390741426 2312 66-67 1.039770721612932 2312 68-69 1.0489287449821205 2312 70-71 1.0824055652365274 2312 72-73 1.0627167136241482 2312 74-75 1.0618052614505444 2312 76-77 1.1436426981999013 2312 78-79 1.1058943724052739 2312 80-81 1.0642743489622255 2312 82-83 1.0711803125098989 2312 84-85 1.0596306343249218 2312 86-87 1.1886874363933515 2312 88-89 1.158670816451835 2312 90-91 1.2090541194045983 2312 92-93 1.1921092670383082 2312 94-95 1.2225935495664686 2312 96-97 1.3865726949370227 2312 98-99 1.4490010433299858 2312 100 1.4063767029438878 2313 1 0.5981507594921531 2313 2 0.5495891385863345 2313 3 0.5966259248980066 2313 4 0.5132645031555043 2313 5 0.5445968749574774 2313 6 0.47767482305783204 2313 7 0.6246875210140672 2313 8 0.5368987842135979 2313 9 0.6960549113365531 2313 10-11 0.6774803168341279 2313 12-13 0.7397702449133163 2313 14-15 0.8209173445905975 2313 16-17 0.8858972224543322 2313 18-19 0.8202770849455376 2313 20-21 0.7969873693486775 2313 22-23 0.7346798717266481 2313 24-25 0.7905709782424708 2313 26-27 0.8880627129587353 2313 28-29 0.956793816028302 2313 30-31 1.0267067697044965 2313 32-33 1.1101126998874449 2313 34-35 0.9926205632649641 2313 36-37 1.1647717969125324 2313 38-39 1.0766866478455697 2313 40-41 1.1125196539947453 2313 42-43 0.8929743191494239 2313 44-45 1.0533737737852675 2313 46-47 1.0020072940059137 2313 48-49 0.9579490169155065 2313 50-51 0.9390896885020439 2313 52-53 0.948302612026815 2313 54-55 0.9861295624621391 2313 56-57 0.9673847448915254 2313 58-59 1.0126197177948484 2313 60-61 1.0618878198939683 2313 62-63 0.9910244016760288 2313 64-65 1.0136588621276488 2313 66-67 0.9225919661219422 2313 68-69 1.0448948997329097 2313 70-71 1.0276706027654967 2313 72-73 1.0802128223716778 2313 74-75 1.052376583784607 2313 76-77 1.1235731744891986 2313 78-79 0.9800458199511937 2313 80-81 1.0070328145981442 2313 82-83 1.1025333442279255 2313 84-85 1.2421436916387485 2313 86-87 1.2150503259509584 2313 88-89 1.1369184557586713 2313 90-91 1.2138592108922666 2313 92-93 1.2478796485539938 2313 94-95 1.1854613161604952 2313 96-97 1.233284673076831 2313 98-99 1.2603153354679257 2313 100 1.4185649703853471 2314 1 0.31317349987839904 2314 2 0.135272538104374 2314 3 0.19194836321046793 2314 4 0.12323701104676132 2314 5 0.14600660920042685 2314 6 0.23925952251138227 2314 7 0.23014826839213498 2314 8 0.20963034583325424 2314 9 0.26222807768078127 2314 10-11 0.3530954103412398 2314 12-13 0.27228297274144353 2314 14-15 0.36803423696407833 2314 16-17 0.483513385099009 2314 18-19 0.4966870737450435 2314 20-21 0.4436508964844066 2314 22-23 0.40535614366093853 2314 24-25 0.4727425154246987 2314 26-27 0.48570288877245815 2314 28-29 0.5282436005037496 2314 30-31 0.5301950431321885 2314 32-33 0.5971936149334311 2314 34-35 0.5332662884117596 2314 36-37 0.5253739080349646 2314 38-39 0.6850693651520245 2314 40-41 0.7042259466329739 2314 42-43 0.5496454829820223 2314 44-45 0.6795358244929304 2314 46-47 0.533129124776714 2314 48-49 0.4802618329898962 2314 50-51 0.5332466820193318 2314 52-53 0.5526839547278328 2314 54-55 0.49510548141132915 2314 56-57 0.43107293007293634 2314 58-59 0.6100391930689284 2314 60-61 0.4529645941532081 2314 62-63 0.5596160250680811 2314 64-65 0.5885523726323498 2314 66-67 0.6959234327071542 2314 68-69 0.6006102885256297 2314 70-71 0.4738035491728567 2314 72-73 0.6596021411783184 2314 74-75 0.7896590227758864 2314 76-77 0.730439074092093 2314 78-79 0.8416416350413058 2314 80-81 0.7839998580085208 2314 82-83 0.7377277405894667 2314 84-85 0.8222181070071777 2314 86-87 0.8502289398056249 2314 88-89 0.8241468439065187 2314 90-91 0.749616431813422 2314 92-93 0.8186987267198305 2314 94-95 0.7470827566112312 2314 96-97 0.764380623930446 2314 98-99 0.7511870219900025 2314 100 0.8481249269410256 2315 1 0.2896712704231277 2315 2 0.21252944809259589 2315 3 0.319806961498351 2315 4 0.261088133713109 2315 5 0.2734153470773535 2315 6 0.16343753492223811 2315 7 0.1895192576880973 2315 8 0.19141364542705475 2315 9 -0.06616076048855035 2315 10-11 0.25443192577542106 2315 12-13 0.16042733162419864 2315 14-15 0.3775090910902179 2315 16-17 0.2774300153799061 2315 18-19 0.3916256479521678 2315 20-21 0.27754374370386614 2315 22-23 0.2373991053301836 2315 24-25 0.3323887134426684 2315 26-27 0.3786534768987977 2315 28-29 0.3969190923731887 2315 30-31 0.3841557994269209 2315 32-33 0.3585909607570059 2315 34-35 0.43771759102227037 2315 36-37 0.5480842508550552 2315 38-39 0.5204621329843562 2315 40-41 0.6442894894160176 2315 42-43 0.6594334201074119 2315 44-45 0.6300748300367971 2315 46-47 0.6946037076634966 2315 48-49 0.7678372507646856 2315 50-51 0.7157710935622177 2315 52-53 0.38205967417371056 2315 54-55 0.6279411566650452 2315 56-57 0.6046704240858247 2315 58-59 0.5702996490156806 2315 60-61 0.751629898007451 2315 62-63 0.7606338342478765 2315 64-65 0.6718570935204298 2315 66-67 0.5029174389787343 2315 68-69 0.5801712446113214 2315 70-71 0.5984406838266665 2315 72-73 0.6086321296671784 2315 74-75 0.37018056315086056 2315 76-77 0.6143936117282074 2315 78-79 0.7820895438519528 2315 80-81 0.6916643547602668 2315 82-83 0.6281634105506981 2315 84-85 0.7232331045594087 2315 86-87 0.608617017181615 2315 88-89 0.7067465078343282 2315 90-91 0.845977854960065 2315 92-93 0.8346973837541931 2315 94-95 0.9145831535468893 2315 96-97 0.8839564981812273 2315 98-99 1.035734151483645 2315 100 1.0447870288476402 2316 1 -0.045131282786393 2316 2 -0.014399106691087127 2316 3 -0.0222984260214254 2316 4 -0.060959045583537375 2316 5 -0.09520924736353464 2316 6 -0.14196367324060333 2316 7 -0.13695855816728653 2316 8 -0.07228076791977145 2316 9 -0.04291935117164769 2316 10-11 -0.07120747656365722 2316 12-13 -0.040380242288996726 2316 14-15 -0.2218359441818052 2316 16-17 -0.03239767393940696 2316 18-19 5.314083174496886E-4 2316 20-21 0.08700718349697922 2316 22-23 -0.04899912235000414 2316 24-25 -0.10642901757732659 2316 26-27 -0.2395748469748895 2316 28-29 -0.1408514772754259 2316 30-31 -0.7454905106573406 2316 32-33 -0.5449014918917712 2316 34-35 -0.5944642070121375 2316 36-37 -0.36591998439611473 2316 38-39 -0.34496540902187434 2316 40-41 -0.11256957237562659 2316 42-43 -0.21925364388088298 2316 44-45 -0.3128606601947439 2316 46-47 -0.6046861259411642 2316 48-49 -0.49095790256966154 2316 50-51 -0.2334860671736223 2316 52-53 -0.5408907347667622 2316 54-55 -0.5260800912918882 2316 56-57 -0.5465262502887 2316 58-59 -0.7586933666493678 2316 60-61 -0.3571947097959054 2316 62-63 -0.487243407021289 2316 64-65 -0.6619015981959819 2316 66-67 -0.8464014417025609 2316 68-69 -0.9144105182244004 2316 70-71 -0.7351632802641426 2316 72-73 -0.9588258152175158 2316 74-75 -0.7100342629985157 2316 76-77 -0.3938127374005411 2316 78-79 -0.35175563726654957 2316 80-81 -0.4659437386596963 2316 82-83 -0.2409659430479607 2316 84-85 -0.19512688853121674 2316 86-87 -0.17358107654796484 2316 88-89 -0.0727833287687325 2316 90-91 -0.7555158491981082 2316 92-93 -0.8007817259793875 2316 94-95 -0.33725267873129994 2316 96-97 -0.01948771920986303 2316 98-99 -0.049364168593136526 2316 100 -0.14018565141941153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 87.0 1 203.0 2 134.0 3 112.0 4 121.0 5 102.0 6 131.0 7 448.0 8 1171.0 9 2047.0 10 3108.0 11 5431.0 12 8644.0 13 10783.0 14 12540.0 15 13905.0 16 16273.0 17 19506.0 18 23266.0 19 26359.0 20 30176.0 21 34512.0 22 37370.0 23 38012.0 24 37938.0 25 39273.0 26 42671.0 27 47930.0 28 55138.0 29 64619.0 30 76825.0 31 93295.0 32 114887.0 33 144673.0 34 195583.0 35 264016.0 36 372680.0 37 527825.0 38 484932.0 39 105087.0 40 83.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18203439784791 18.904178101385448 13.017535739178964 22.89625176158768 2 16.23283362413797 20.665001534566752 40.39406995928735 22.708094882007927 3 18.482540700079657 27.532393871804196 30.028152674300767 23.956912753815384 4 12.507568531848822 17.261504422529914 38.70771659892965 31.523210446691614 5 12.810549377867803 37.11792286929633 34.673306899802185 15.398220853033678 6 28.85068628441166 36.975440989165996 19.840360667894473 14.33351205852787 7 26.624651545064015 32.59470772150719 23.35669075842435 17.423949975004447 8 25.78412929292381 34.39561441352168 21.48475797905419 18.335498314500313 9 26.868308287684115 16.604308111469376 21.48626965260281 35.0411139482437 10-11 24.36368990804764 26.22603569887233 29.05294339220451 20.35733100087552 12-13 25.01357229611395 24.519259444013684 28.786518118083382 21.68065014178898 14-15 22.890193429257547 25.760430745697484 26.962999184506952 24.386376640538018 16-17 21.692001279665117 28.193028807376507 27.75499089729739 22.359979015660986 18-19 21.36325295672351 27.434605399114893 29.887858176227127 21.314283467934477 20-21 22.303757337397776 26.86095116468003 29.73878372474595 21.096507773176246 22-23 22.231780006910753 26.60026378807883 29.431604984215248 21.736351220795164 24-25 21.804906598488675 26.76038767758711 29.708360431475306 21.72634529244891 26-27 21.598164848406785 26.906884869151387 29.6525448576605 21.842405424781326 28-29 21.499960090136977 26.922329305685317 29.890216524988066 21.687494079189644 30-31 22.28817066396245 26.775701900691185 29.664970091033478 21.27115734431289 32-33 22.0435565727579 27.006942541567724 29.442110719718674 21.5073901659557 34-35 22.22190213010197 27.057136499951955 29.44205627479314 21.278905095152936 36-37 22.1844627949048 26.977650711797686 29.134547147827394 21.703339345470123 38-39 21.974154552778323 27.352324439704066 29.12862700679435 21.544894000723257 40-41 22.082473094625637 26.7988409106085 29.56725318253014 21.55143281223572 42-43 21.82458816351434 27.003491288726188 29.10833163853298 22.063588909226493 44-45 21.671566732386925 27.27574254766018 29.199911664924656 21.852779055028243 46-47 21.767874690813365 26.87353457314194 28.850701964252483 22.507888771792214 48-49 21.814691020914754 27.124131108342464 28.860990724195446 22.20018714654734 50-51 21.86378027044373 27.26650294317577 28.664750815976483 22.20496597040402 52-53 21.894657199374986 27.562506853775226 28.373146387486308 22.16968955936348 54-55 21.783515675655156 27.353314106581372 28.594941097877136 22.268229119886335 56-57 21.955366314262758 27.449220162083993 28.255521842901672 22.33989168075158 58-59 21.730748177068946 27.33779701328427 28.64612479074382 22.285330018902965 60-61 22.213526789943344 27.624100416765028 28.335547957126057 21.826824836165574 62-63 21.996696227303087 27.45859818961933 28.524655509584147 22.020050073493437 64-65 21.842564805142246 27.08454648433078 28.456387235764698 22.61650147476228 66-67 21.80637774569516 27.206838738957522 28.219780003752078 22.767003511595245 68-69 21.494611958109147 27.34213265211179 28.465534683268757 22.697720706510307 70-71 21.768370023327122 26.928249950681543 28.611483777109047 22.691896248882284 72-73 21.651676835210605 27.2964135925733 28.21732809375647 22.834581478459622 74-75 21.74920862653426 27.420609722348615 28.12097036732371 22.70921128379341 76-77 21.60184965032654 27.150173595370518 28.366705412884475 22.88127134141846 78-79 21.534863185333894 27.018856348202203 28.359215509475632 23.087064956988275 80-81 21.57134290757267 27.389122084941064 27.985548812587098 23.05398619489917 82-83 21.995815401802435 27.19200784451164 28.193382938865074 22.618793814820854 84-85 21.79199994576579 27.40225766842052 27.855553964308804 22.95018842150488 86-87 21.438466487201406 27.464523297379266 28.345670157674984 22.751340057744336 88-89 21.632476791262974 26.953931337087315 28.366463894064463 23.04712797758524 90-91 21.95455866786334 27.002938287487744 28.24637544822232 22.796127596426594 92-93 21.870227312561468 27.005889019560115 28.21988272135015 22.90400094652827 94-95 21.596714708261015 26.755935444817432 28.329267571220335 23.31808227570122 96-97 21.713629964751043 26.753493749009444 28.269343067549347 23.26353321869017 98-99 21.562099819864905 26.790323493881747 28.183971739844644 23.463604946408704 100 21.852572952034652 26.574586947157048 28.006560001628127 23.56628009918017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1673.0 1 1196.5 2 2050.5 3 2889.5 4 1735.0 5 1184.5 6 1575.5 7 2554.5 8 3986.5 9 4659.5 10 3944.5 11 3119.0 12 3006.5 13 3225.5 14 3530.5 15 3741.0 16 3949.0 17 4193.0 18 4495.0 19 4849.0 20 5327.5 21 5885.0 22 6557.5 23 7672.5 24 8895.0 25 10482.5 26 13063.0 27 16185.0 28 19114.0 29 23452.0 30 30246.5 31 36701.0 32 44638.5 33 55066.0 34 65686.5 35 75014.5 36 86265.5 37 97522.5 38 101663.0 39 103094.0 40 101237.0 41 98364.5 42 97516.5 43 98202.5 44 101638.5 45 106175.0 46 108503.5 47 107982.0 48 108801.0 49 110331.0 50 109497.5 51 105661.0 52 100793.5 53 96278.5 54 92263.5 55 85961.5 56 77819.0 57 69152.5 58 60571.5 59 52375.5 60 43153.0 61 34587.0 62 27481.5 63 21220.5 64 15764.5 65 11592.5 66 8600.0 67 6488.5 68 5041.0 69 3848.0 70 2928.0 71 2236.0 72 1727.0 73 1356.0 74 1016.5 75 759.0 76 558.0 77 341.0 78 207.5 79 151.5 80 109.5 81 80.5 82 63.5 83 47.0 84 34.0 85 27.0 86 22.5 87 16.0 88 14.5 89 13.5 90 9.5 91 4.5 92 4.0 93 5.0 94 3.0 95 2.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024492732806304828 2 0.10776124904129412 3 0.016565624263185426 4 0.01798843861707865 5 0.02012266014791849 6 0.04641084916270763 7 0.046444725694943186 8 0.038585370216294886 9 0.03221658215601092 10-11 0.03640033388710171 12-13 0.03496058126709071 14-15 0.031183347922826548 16-17 0.03807722223276159 18-19 0.036552778282161705 20-21 0.048409564564605254 22-23 0.04815549057283861 24-25 0.06675370677015721 26-27 0.05864027729974226 28-29 0.05073010702274064 30-31 0.048883836015903 32-33 0.0447000842848122 34-35 0.04595351597752766 36-37 0.04058408561819251 38-39 0.04597045424364544 40-41 0.055862401656426926 42-43 0.057454598671497915 44-45 0.059809017661868843 46-47 0.061553659071999826 48-49 0.06102857282234875 50-51 0.054083883714060386 52-53 0.0636709423367219 54-55 0.050577662627680646 56-57 0.05540506847124695 58-59 0.05301677294864047 60-61 0.04852813242742969 62-63 0.05530343887454029 64-65 0.05386368625452929 66-67 0.05174640298980723 68-69 0.05777642572773567 70-71 0.056980327220200175 72-73 0.06046961004046213 74-75 0.05039134170038511 76-77 0.050831736619447294 78-79 0.061943239192708686 80-81 0.061892424394355355 82-83 0.052610254561813834 84-85 0.05855558596915339 86-87 0.055896278188662475 88-89 0.06353543620777967 90-91 0.06939607628453034 92-93 0.0734781984189145 94-95 0.07671340724740981 96-97 0.07329187749161895 98-99 0.08276036825145602 100 0.12622395910967052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2951896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.55162397766206 #Duplication Level Percentage of deduplicated Percentage of total 1 83.45778254532297 55.54250961965822 2 10.796814077468524 14.370910212808269 3 2.686470464511964 5.363669165438861 4 1.028544893409789 2.7380533196141106 5 0.565263646254803 1.8809606816895912 6 0.31485228762369954 1.2572358632663492 7 0.21209271980237998 0.9880580455681344 8 0.16372943463251505 0.8717167814190673 9 0.11102639634602357 0.665008828309389 >10 0.5793563240789791 6.921241026205828 >50 0.045574985712837725 2.124471542993929 >100 0.035421719404649527 4.425638217425461 >500 0.002433230718771744 1.0977296103318488 >1k 4.6347251785474545E-4 0.6613906689412553 >5k 5.793406473184318E-5 0.21934543054720773 >10k+ 1.1586812946368636E-4 0.872060985782518 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12610 0.42718307149032353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10008 0.33903633461341454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5689 0.1927235918880611 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.1591180719103925 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.387653223555301E-5 0.0 5 0.0 0.0 0.0 3.387653223555301E-5 0.0 6 0.0 0.0 0.0 3.387653223555301E-5 3.387653223555301E-5 7 0.0 0.0 0.0 3.387653223555301E-5 3.387653223555301E-5 8 0.0 0.0 0.0 3.387653223555301E-5 3.387653223555301E-5 9 0.0 0.0 0.0 3.387653223555301E-5 3.387653223555301E-5 10-11 0.0 0.0 0.0 3.387653223555301E-5 3.387653223555301E-5 12-13 0.0 0.0 0.0 5.081479835332952E-5 3.387653223555301E-5 14-15 0.0 5.081479835332952E-5 0.0 6.775306447110603E-5 6.775306447110603E-5 16-17 0.0 1.0162959670665904E-4 0.0 6.775306447110603E-5 1.0162959670665904E-4 18-19 0.0 1.0162959670665904E-4 0.0 1.1856786282443555E-4 1.1856786282443555E-4 20-21 0.0 1.0162959670665904E-4 0.0 2.032591934133181E-4 1.3550612894221205E-4 22-23 0.0 1.0162959670665904E-4 0.0 6.097775802399543E-4 1.3550612894221205E-4 24-25 0.0 1.0162959670665904E-4 0.0 0.0016938266117776507 1.3550612894221205E-4 26-27 0.0 1.0162959670665904E-4 0.0 0.003472344554144184 1.3550612894221205E-4 28-29 0.0 1.1856786282443555E-4 0.0 0.007740787615823864 1.3550612894221205E-4 30-31 0.0 1.3550612894221205E-4 0.0 0.01973308002720963 1.3550612894221205E-4 32-33 0.0 1.3550612894221205E-4 0.0 0.048121614040603056 1.5244439505998858E-4 34-35 0.0 1.6938266117776508E-4 0.0 0.09009463748045324 2.032591934133181E-4 36-37 0.0 1.6938266117776508E-4 0.0 0.1590503188459214 2.032591934133181E-4 38-39 0.0 1.6938266117776508E-4 0.0 0.2646773463563757 2.710122578844241E-4 40-41 0.0 1.6938266117776508E-4 0.0 0.38424456688176006 2.710122578844241E-4 42-43 0.0 1.6938266117776508E-4 0.0 0.499899047933938 2.710122578844241E-4 44-45 0.0 1.6938266117776508E-4 0.0 0.6404697184453653 2.710122578844241E-4 46-47 0.0 1.6938266117776508E-4 0.0 0.8090224045833594 2.710122578844241E-4 48-49 0.0 1.6938266117776508E-4 0.0 0.9862982977720083 2.710122578844241E-4 50-51 0.0 1.6938266117776508E-4 0.0 1.171010089786361 2.710122578844241E-4 52-53 0.0 1.6938266117776508E-4 0.0 1.3585336339762648 2.710122578844241E-4 54-55 0.0 1.6938266117776508E-4 0.0 1.5749877366953307 2.710122578844241E-4 56-57 0.0 1.6938266117776508E-4 0.0 1.8135124001658594 2.710122578844241E-4 58-59 0.0 1.6938266117776508E-4 0.0 2.0786301414412973 2.710122578844241E-4 60-61 0.0 2.032591934133181E-4 0.0 2.3417491673148376 2.710122578844241E-4 62-63 0.0 2.201974595310946E-4 0.0 2.61574256003599 3.048887901199771E-4 64-65 0.0 2.371357256488711E-4 0.0 2.8958506668256607 3.218270562377536E-4 66-67 0.0 2.710122578844241E-4 0.0 3.2078704669812215 3.3876532235553017E-4 68-69 0.0 2.710122578844241E-4 0.0 3.5533941575177446 3.3876532235553017E-4 70-71 0.0 2.710122578844241E-4 0.0 3.916516706550637 3.3876532235553017E-4 72-73 0.0 2.710122578844241E-4 0.0 4.26783328409944 3.726418545910832E-4 74-75 0.0 2.710122578844241E-4 0.0 4.625501711442409 3.726418545910832E-4 76-77 0.0 2.710122578844241E-4 0.0 5.010186673243231 3.895801207088597E-4 78-79 0.0 2.710122578844241E-4 0.0 5.436167127839193 4.065183868266362E-4 80-81 0.0 2.710122578844241E-4 0.0 5.865806247916593 4.403949190621892E-4 82-83 0.0 2.710122578844241E-4 0.0 6.299459737063907 5.420245157688482E-4 84-85 0.0 2.710122578844241E-4 0.0 6.741768680197405 5.420245157688482E-4 86-87 3.387653223555301E-5 2.710122578844241E-4 0.0 7.2102472444828685 5.420245157688482E-4 88 3.387653223555301E-5 2.710122578844241E-4 0.0 7.575334632385423 5.420245157688482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5685 0.0 52.53075 1 TACCTGG 3170 0.0 52.516968 2 ACCTGGG 3705 0.0 43.502625 3 GTACCTG 4080 0.0 41.842426 1 GTATCAA 10180 0.0 39.91502 1 ATCAACG 12305 0.0 32.230743 3 CAACGCA 12315 0.0 32.16696 5 TCAACGC 12435 0.0 31.856005 4 TATCAAC 12745 0.0 31.29044 2 AACGCAG 13140 0.0 30.826822 6 TAACGCA 415 0.0 29.439793 4 GTACATG 11200 0.0 28.13372 1 ACGCAGA 14675 0.0 27.282598 7 CCTGGGG 3400 0.0 27.088606 4 CGCAGAG 15030 0.0 26.577017 8 TACATGG 11765 0.0 26.262077 2 ACATGGG 11695 0.0 25.916061 3 GCAGAGT 16030 0.0 23.83435 9 TATAACG 535 0.0 23.733711 2 CATGGGG 7285 0.0 23.479094 4 >>END_MODULE