FastQCFastQC Report
Mon 6 Jun 2016
ERR1211317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951896
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.39249350248111725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97710.3310075964735885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57170.19367213479065656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36750.12449625596565733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG32600.053.6796762
GGTATCA56650.053.5141941
ACCTGGG38100.044.7060743
GTACCTG42450.041.7878951
GTATCAA101950.038.9881441
TAACGCA3200.038.124864
CCTGGGG35500.031.986884
ATCAACG125950.030.9963133
CAACGCA127250.030.599685
TCAACGC128700.030.261084
TATCAAC131600.030.0531732
AACGCAG135100.029.1332196
TATAACG4350.026.9630742
ACGCAGA152650.025.5994157
GTACATG120050.024.861731
CGCAGAG157000.024.8004938
CTGGGGG24900.023.7392925
TACATGG126400.023.0867042
ACATGGG121450.022.9495323
TATCACG2053.0477167E-822.885732
Produced by FastQC (version 0.11.5)