FastQCFastQC Report
Mon 6 Jun 2016
ERR1211316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206680.3361199675719366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166490.2707596932506857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92880.15104907387304753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110200.052.8450971
GTATCAA197050.040.6540531
CAACGCA241550.032.3110435
ATCAACG242400.032.2370343
TATCAAC248750.031.5978722
TCAACGC253000.030.9418564
AACGCAG258800.030.4846956
TACCTGG45950.028.150862
GTACATG276500.026.8798981
ACGCAGA287450.026.867
CGCAGAG296400.025.9707288
TACATGG283800.025.7231272
ACATGGG285450.024.9224823
GTACTAG22300.024.6800861
GTGGTAT52300.024.5542491
TGGTATC53000.023.3412632
GCAGAGT320250.023.3181349
CATGGGG193600.023.1302034
GTACCTG59950.022.4410151
GAGTACT168500.022.18577612-13
Produced by FastQC (version 0.11.5)