##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211316_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6148995 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15926196069439 31.0 31.0 34.0 28.0 34.0 2 31.169852146570292 31.0 31.0 34.0 27.0 34.0 3 31.214710046113225 31.0 31.0 34.0 27.0 34.0 4 34.816375521528315 37.0 35.0 37.0 32.0 37.0 5 34.8595095621317 37.0 35.0 37.0 32.0 37.0 6 34.927388784671315 37.0 35.0 37.0 32.0 37.0 7 34.84973251726502 37.0 35.0 37.0 32.0 37.0 8 34.846280896309075 37.0 35.0 37.0 32.0 37.0 9 36.359262123322594 39.0 37.0 39.0 32.0 39.0 10-11 36.279056252281876 39.0 36.0 39.0 31.0 39.0 12-13 36.191082363865966 39.0 35.0 39.0 31.0 39.0 14-15 37.28241151602823 40.0 37.0 41.0 31.0 41.0 16-17 37.2719420815922 40.0 37.0 41.0 31.0 41.0 18-19 37.27287434775927 40.0 37.0 41.0 31.0 41.0 20-21 37.23439993039513 40.0 37.0 41.0 31.0 41.0 22-23 37.11401871362719 40.0 36.0 41.0 30.5 41.0 24-25 36.99826882279137 40.0 36.0 41.0 30.0 41.0 26-27 36.85140376598127 39.5 36.0 41.0 30.0 41.0 28-29 36.708462439797074 39.0 36.0 41.0 30.0 41.0 30-31 36.51518174921267 39.0 35.5 41.0 29.0 41.0 32-33 36.38382922737781 39.0 35.0 41.0 29.0 41.0 34-35 36.23997669537867 39.0 35.0 40.5 28.5 41.0 36-37 36.14408883077641 39.0 35.0 40.0 28.0 41.0 38-39 35.992716777294504 39.0 35.0 40.0 27.0 41.0 40-41 35.82565012656539 38.0 35.0 40.0 27.0 41.0 42-43 35.67257210324614 38.0 34.0 40.0 27.0 41.0 44-45 35.53524933749336 38.0 34.0 40.0 26.0 41.0 46-47 35.36679741974095 38.0 34.0 40.0 25.5 41.0 48-49 35.12753685764909 38.0 33.5 40.0 24.0 41.0 50-51 34.468034451158275 37.5 32.5 39.5 24.0 40.5 52-53 34.72888707829491 38.0 33.5 40.0 24.0 40.5 54-55 35.08627857072578 38.0 34.0 40.0 25.0 41.0 56-57 34.9866415568723 38.0 34.0 40.0 25.0 41.0 58-59 34.76638995478123 38.0 33.0 40.0 24.0 41.0 60-61 34.500720849504674 37.0 33.0 40.0 24.0 41.0 62-63 34.21810946341638 37.0 33.0 40.0 23.5 41.0 64-65 33.938758528832764 36.0 32.5 39.5 23.0 41.0 66-67 33.71125240791382 36.0 32.0 39.0 23.0 41.0 68-69 33.368318969197404 35.0 32.0 39.0 22.0 41.0 70-71 33.02256767162764 35.0 31.5 38.5 22.0 40.0 72-73 32.656654867990625 35.0 31.0 37.5 21.5 40.0 74-75 32.283863623242496 35.0 31.0 37.0 20.5 39.0 76-77 31.92691821996928 35.0 31.0 36.5 20.0 39.0 78-79 31.564205044889448 35.0 30.0 36.0 20.0 39.0 80-81 31.239191201163766 34.0 30.0 35.5 19.5 37.5 82-83 30.928994250279924 34.0 30.0 35.0 18.5 37.0 84-85 30.625617926181434 34.0 29.0 35.0 18.0 36.5 86-87 30.375057224798525 34.0 29.0 35.0 18.0 36.0 88-89 30.115518633532798 34.0 29.0 35.0 17.5 36.0 90-91 29.85848150795374 34.0 29.0 35.0 16.5 35.5 92-93 29.619519449926372 34.0 28.5 35.0 15.0 35.0 94-95 29.39675264006557 34.0 27.0 35.0 14.0 35.0 96-97 29.18918538720555 34.0 27.0 35.0 12.0 35.0 98-99 28.94894905590263 33.0 26.5 35.0 10.5 35.0 100 27.240174207329815 31.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4421602907272053 1101 2 -0.4447397856226658 1101 3 -0.41618358890764995 1101 4 -0.35052776751371084 1101 5 -0.4055413235502243 1101 6 -0.38229462424703087 1101 7 -0.4335789078591219 1101 8 -0.6152039281554877 1101 9 -0.6027852513138541 1101 10-11 -0.6091537716100177 1101 12-13 -0.6280908016537481 1101 14-15 -0.7126787618903947 1101 16-17 -0.6437706978264615 1101 18-19 -0.7416610400332431 1101 20-21 -0.8502495493015445 1101 22-23 -0.7258164847460691 1101 24-25 -0.6944551090653803 1101 26-27 -0.8342488531117738 1101 28-29 -0.8397982525863412 1101 30-31 -0.9978586601855923 1101 32-33 -0.882519310164902 1101 34-35 -0.8298452824290621 1101 36-37 -0.9568699867281296 1101 38-39 -1.0709428135179095 1101 40-41 -0.9801748260690317 1101 42-43 -1.0971155477503771 1101 44-45 -1.0937103476980425 1101 46-47 -0.9982868202519057 1101 48-49 -1.0506201546385583 1101 50-51 -0.9024102617761329 1101 52-53 -0.6881060593125667 1101 54-55 -0.699817324845931 1101 56-57 -0.7373719038397013 1101 58-59 -0.8041427305111171 1101 60-61 -0.7558273801851527 1101 62-63 -0.781831341604601 1101 64-65 -0.759245514128601 1101 66-67 -0.8420486823458901 1101 68-69 -0.7959548828302232 1101 70-71 -0.8659871015637819 1101 72-73 -0.9875952887949531 1101 74-75 -0.9246974121728471 1101 76-77 -0.9103871988587819 1101 78-79 -0.9501546238766174 1101 80-81 -0.9953247965172132 1101 82-83 -1.114172285524596 1101 84-85 -1.131291171784401 1101 86-87 -1.0618542756879528 1101 88-89 -1.1606237697109485 1101 90-91 -1.1840067984320157 1101 92-93 -1.1735287397202434 1101 94-95 -1.1957894054732243 1101 96-97 -1.340459248231955 1101 98-99 -1.3854793314848024 1101 100 -1.457200132132897 1102 1 -0.404052065658675 1102 2 -0.4279370268852851 1102 3 -0.38982830563154636 1102 4 -0.3242650100837636 1102 5 -0.27955389558830746 1102 6 -0.32338515138108903 1102 7 -0.33772931915247995 1102 8 -0.2981635247096719 1102 9 -0.343875985507033 1102 10-11 -0.4071734434094836 1102 12-13 -0.5185327900483827 1102 14-15 -0.5707836473911456 1102 16-17 -0.6181854230427462 1102 18-19 -0.5720686921100366 1102 20-21 -0.5974386921862447 1102 22-23 -0.6868934228859729 1102 24-25 -0.6883707803939245 1102 26-27 -0.6733333027649024 1102 28-29 -0.6986960857718287 1102 30-31 -0.7574886987843712 1102 32-33 -0.7438855690532833 1102 34-35 -0.7961081640944769 1102 36-37 -0.8178314450395945 1102 38-39 -0.8481494647300778 1102 40-41 -0.8861550631635566 1102 42-43 -0.8200343891540385 1102 44-45 -0.7955548007469488 1102 46-47 -0.8275085560166673 1102 48-49 -0.888272686956789 1102 50-51 -0.8803404958683032 1102 52-53 -0.7835600319931899 1102 54-55 -0.6795474646966184 1102 56-57 -0.5610642589508217 1102 58-59 -0.6300062188005953 1102 60-61 -0.727593730534501 1102 62-63 -0.6986758079644417 1102 64-65 -0.7258427075309797 1102 66-67 -0.6016195611964861 1102 68-69 -0.6592096096201558 1102 70-71 -0.6587881440768655 1102 72-73 -0.7606653129350889 1102 74-75 -0.7528907888249208 1102 76-77 -0.7433074837602973 1102 78-79 -0.7515381781173822 1102 80-81 -0.7764270875671997 1102 82-83 -0.8866200082736206 1102 84-85 -0.8794833090397525 1102 86-87 -0.8407954315570265 1102 88-89 -0.9608313316874408 1102 90-91 -1.0693615513409753 1102 92-93 -1.1588882254090542 1102 94-95 -1.1130424586338776 1102 96-97 -1.0877690622130025 1102 98-99 -1.0494339476755883 1102 100 -1.1339288169495774 1103 1 -0.4593172032863997 1103 2 -0.4982054101713871 1103 3 -0.4379629981641706 1103 4 -0.43251097096857904 1103 5 -0.33104777825143117 1103 6 -0.5088865194266958 1103 7 -0.4443296119246085 1103 8 -0.4552185591681237 1103 9 -0.7494067175409995 1103 10-11 -0.6446968216710189 1103 12-13 -0.7561741895116754 1103 14-15 -0.8638513525720697 1103 16-17 -0.7848792674280674 1103 18-19 -0.7626534083734384 1103 20-21 -0.850832472140489 1103 22-23 -0.8424091197961943 1103 24-25 -0.8261648873003438 1103 26-27 -0.8127834728094427 1103 28-29 -0.8340572143787242 1103 30-31 -0.9832902245451152 1103 32-33 -0.9448267135642681 1103 34-35 -1.0436794040661468 1103 36-37 -0.9874968204331864 1103 38-39 -1.0990347292000067 1103 40-41 -0.9556357445662087 1103 42-43 -0.9410640016588303 1103 44-45 -1.019069541435357 1103 46-47 -0.9993931940130736 1103 48-49 -1.0262602317277114 1103 50-51 -0.9753210785634181 1103 52-53 -0.8090771378059856 1103 54-55 -0.8145118950287298 1103 56-57 -0.8430618345295855 1103 58-59 -0.8932651490614845 1103 60-61 -0.8710810874115751 1103 62-63 -0.9467314561210998 1103 64-65 -0.9757183287919418 1103 66-67 -0.9152014724300841 1103 68-69 -0.9264786695169605 1103 70-71 -0.8736016760015843 1103 72-73 -1.0315312926743658 1103 74-75 -0.9810546971037972 1103 76-77 -0.8784032228484122 1103 78-79 -0.9043065451330072 1103 80-81 -0.8932819072071787 1103 82-83 -0.9966061265457462 1103 84-85 -1.0360139521284495 1103 86-87 -1.1719309250790886 1103 88-89 -1.080783085512028 1103 90-91 -1.0135097124519383 1103 92-93 -1.0448448533857366 1103 94-95 -1.277135889483997 1103 96-97 -1.3375982523603724 1103 98-99 -1.4001298660040717 1103 100 -1.406969375747579 1104 1 -0.47528414318057344 1104 2 -0.426676929508254 1104 3 -0.4659715034038996 1104 4 -0.3186994623387065 1104 5 -0.25304032922583275 1104 6 -0.34505008414335236 1104 7 -0.3505230101638048 1104 8 -0.4756855144528487 1104 9 -0.46639860762712715 1104 10-11 -0.5053791903187275 1104 12-13 -0.4948465192967362 1104 14-15 -0.7067999161510272 1104 16-17 -0.6276717527361342 1104 18-19 -0.7311808980059453 1104 20-21 -0.7898602481579147 1104 22-23 -0.6417378957164459 1104 24-25 -0.6903246448177214 1104 26-27 -0.7982217956379714 1104 28-29 -0.7611485636228608 1104 30-31 -0.8183277442887658 1104 32-33 -0.7838354475497908 1104 34-35 -0.7689706324793875 1104 36-37 -0.8408425144895944 1104 38-39 -0.8384943609977356 1104 40-41 -0.84203854178336 1104 42-43 -0.8416242478110405 1104 44-45 -0.8185440899943472 1104 46-47 -0.9221041667500742 1104 48-49 -0.9859301135101859 1104 50-51 -0.8857713818779303 1104 52-53 -0.7905154721019869 1104 54-55 -0.8394746106699316 1104 56-57 -0.6742225655746097 1104 58-59 -0.8011911609598812 1104 60-61 -0.7846081273986343 1104 62-63 -0.7959799295899046 1104 64-65 -0.7549344790906076 1104 66-67 -0.7810893018858991 1104 68-69 -0.6987943918376587 1104 70-71 -0.770031279933356 1104 72-73 -0.8405501542445322 1104 74-75 -0.9242422819404261 1104 76-77 -0.8046209321191355 1104 78-79 -0.7803827518878244 1104 80-81 -0.8719046613187196 1104 82-83 -0.8936872033530356 1104 84-85 -0.9020941785324759 1104 86-87 -0.9139610953830584 1104 88-89 -0.9277527015510323 1104 90-91 -0.8920126466147913 1104 92-93 -0.9542969136370196 1104 94-95 -1.0013631028712418 1104 96-97 -1.0792405657469502 1104 98-99 -1.1275192185206677 1104 100 -1.1136001371693283 1105 1 -0.41129350427561207 1105 2 -0.4705555588572494 1105 3 -0.4139805866252608 1105 4 -0.3742435835060647 1105 5 -0.31124012881996777 1105 6 -0.3470673447900623 1105 7 -0.36636496740494096 1105 8 -0.39246706965528233 1105 9 -0.5013358695248726 1105 10-11 -0.5604508510530124 1105 12-13 -0.5872392913332547 1105 14-15 -0.6324322592035259 1105 16-17 -0.7320826364826871 1105 18-19 -0.7029091760432209 1105 20-21 -0.7696890701539587 1105 22-23 -0.6996856542622609 1105 24-25 -0.6597662991645237 1105 26-27 -0.7259202377410077 1105 28-29 -0.7299649871600877 1105 30-31 -0.7762689920498431 1105 32-33 -0.7345306279451478 1105 34-35 -0.8465938472011558 1105 36-37 -0.8330774939738603 1105 38-39 -0.8381041950794739 1105 40-41 -0.9649135299045639 1105 42-43 -0.9189959627968562 1105 44-45 -0.9215988659932819 1105 46-47 -0.8737514704732234 1105 48-49 -0.905955889000289 1105 50-51 -0.7903392311717639 1105 52-53 -0.7161592127869056 1105 54-55 -0.6530194938848055 1105 56-57 -0.7164742956690588 1105 58-59 -0.6016753347909187 1105 60-61 -0.683348237922587 1105 62-63 -0.6953480526674909 1105 64-65 -0.6767169635459211 1105 66-67 -0.6205623553567392 1105 68-69 -0.6357417720662895 1105 70-71 -0.7516645516372762 1105 72-73 -0.7578088762902588 1105 74-75 -0.7021327395658936 1105 76-77 -0.7134556610359226 1105 78-79 -0.728917855392762 1105 80-81 -0.765872095967282 1105 82-83 -0.7626015593176341 1105 84-85 -0.7531658867819573 1105 86-87 -0.8009549382557175 1105 88-89 -0.8199739226795337 1105 90-91 -0.8167128648238169 1105 92-93 -0.8666183557412594 1105 94-95 -0.9944188472178332 1105 96-97 -1.0016714221151304 1105 98-99 -1.0676565073637363 1105 100 -1.0886566441648888 1106 1 -0.4429640526016705 1106 2 -0.5489273368021763 1106 3 -0.514521543083525 1106 4 -0.35586971134912915 1106 5 -0.3942792579756045 1106 6 -0.35122575658992616 1106 7 -0.4251394379219633 1106 8 -0.43186174576739944 1106 9 -0.5269514398341144 1106 10-11 -0.5283661402982247 1106 12-13 -0.48487933542040906 1106 14-15 -0.6087743061013171 1106 16-17 -0.639887851577825 1106 18-19 -0.6431534814532398 1106 20-21 -0.6606653775388693 1106 22-23 -0.6739519071107125 1106 24-25 -0.6946490595243446 1106 26-27 -0.5948555978464327 1106 28-29 -0.6694231478477448 1106 30-31 -0.7220884726659449 1106 32-33 -0.7740232211496618 1106 34-35 -0.6544747449925055 1106 36-37 -0.712410997114695 1106 38-39 -0.6412785808618295 1106 40-41 -0.732315598319353 1106 42-43 -0.6683430916007893 1106 44-45 -0.6829259079949068 1106 46-47 -0.7442138903581395 1106 48-49 -0.8614787330609701 1106 50-51 -0.7521310034548279 1106 52-53 -0.593191588773081 1106 54-55 -0.6930030916395751 1106 56-57 -0.6379237427922533 1106 58-59 -0.627097788318494 1106 60-61 -0.7107977232102058 1106 62-63 -0.6680469979769157 1106 64-65 -0.729226159238074 1106 66-67 -0.7025827904697408 1106 68-69 -0.620355563865381 1106 70-71 -0.6717556293279543 1106 72-73 -0.62072482616637 1106 74-75 -0.7194901821827919 1106 76-77 -0.6027371240954267 1106 78-79 -0.5171492112295759 1106 80-81 -0.6748597437396633 1106 82-83 -0.7633810313056806 1106 84-85 -0.6773132019152079 1106 86-87 -0.7178533994084475 1106 88-89 -0.8074905350706025 1106 90-91 -0.6876454256417155 1106 92-93 -0.7773427864271873 1106 94-95 -0.8754924847360677 1106 96-97 -0.9742035792597754 1106 98-99 -0.8806613706699054 1106 100 -0.9549506280517726 1107 1 -0.4767085689127484 1107 2 -0.5224693038317021 1107 3 -0.5073004892452744 1107 4 -0.4019938951337281 1107 5 -0.27202379192267045 1107 6 -0.4220509002129731 1107 7 -0.5367522927910358 1107 8 -0.4333429921024248 1107 9 -0.5412171876659926 1107 10-11 -0.5083909629463577 1107 12-13 -0.46184744515149845 1107 14-15 -0.6460447406960625 1107 16-17 -0.5668119154377251 1107 18-19 -0.5566995047591945 1107 20-21 -0.6821766050474807 1107 22-23 -0.7278603939873349 1107 24-25 -0.7077825330637566 1107 26-27 -0.624232386245481 1107 28-29 -0.7520234414868767 1107 30-31 -0.6474595999139794 1107 32-33 -0.726595958563081 1107 34-35 -0.7010613442897053 1107 36-37 -0.688849145512684 1107 38-39 -0.7207404187959483 1107 40-41 -0.8147456606184633 1107 42-43 -0.8069580495610182 1107 44-45 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2311 28-29 0.9143495939884971 2311 30-31 0.9479834013062671 2311 32-33 0.9223779678398429 2311 34-35 0.9636523386518192 2311 36-37 0.9311106818884838 2311 38-39 0.987593796643857 2311 40-41 1.0092578163512087 2311 42-43 1.0392556186787374 2311 44-45 1.0286248240036784 2311 46-47 0.9910950897468922 2311 48-49 0.9779036162036405 2311 50-51 1.0185221064013916 2311 52-53 0.9282462820655439 2311 54-55 0.8649053056821927 2311 56-57 0.8923395412557227 2311 58-59 0.8827558696112803 2311 60-61 0.8339211372676942 2311 62-63 0.8322072566928114 2311 64-65 0.8527475335955828 2311 66-67 0.8148755245074994 2311 68-69 0.8446180488689592 2311 70-71 0.9067409488532334 2311 72-73 0.8899061592858928 2311 74-75 0.8979879149000141 2311 76-77 0.9715241265220129 2311 78-79 0.9590197989491038 2311 80-81 0.9605636357964151 2311 82-83 1.0303371944457673 2311 84-85 1.0818948069248435 2311 86-87 1.0675436747817315 2311 88-89 1.0738762233461934 2311 90-91 1.12355189843408 2311 92-93 1.145572537665597 2311 94-95 1.22505317673264 2311 96-97 1.1913186379865799 2311 98-99 1.1894840945554606 2311 100 1.1924189283800217 2312 1 0.5643068208719519 2312 2 0.5193803711009437 2312 3 0.5929586460598557 2312 4 0.5131241117483469 2312 5 0.4689317695150024 2312 6 0.4923185875717735 2312 7 0.5143633547994497 2312 8 0.5139953474736103 2312 9 0.6551253060520281 2312 10-11 0.6888290736274953 2312 12-13 0.7313791761664916 2312 14-15 0.9407637856290094 2312 16-17 0.8467392776845628 2312 18-19 0.8413514569059402 2312 20-21 0.8113156662178724 2312 22-23 0.836905713435371 2312 24-25 0.8956857826897107 2312 26-27 0.9525451622910168 2312 28-29 0.9787291991187956 2312 30-31 0.9972920640996961 2312 32-33 1.0001029066321792 2312 34-35 0.9270684699281659 2312 36-37 0.9397252718861253 2312 38-39 1.073318420872539 2312 40-41 1.0949829604167007 2312 42-43 1.0506070454935355 2312 44-45 1.0875202340027101 2312 46-47 1.1332665262697361 2312 48-49 1.129344637099912 2312 50-51 1.1505777681977278 2312 52-53 1.0552241363522157 2312 54-55 0.9313302815035414 2312 56-57 0.9046654957343208 2312 58-59 0.862322935379531 2312 60-61 0.959213639041792 2312 62-63 0.9091742110413321 2312 64-65 0.9982189773379275 2312 66-67 0.9464454715856192 2312 68-69 0.9533444744777313 2312 70-71 0.9666542201122255 2312 72-73 0.887025909890653 2312 74-75 0.8751982693172593 2312 76-77 0.9183974079415265 2312 78-79 0.9129176143370081 2312 80-81 1.004450421494898 2312 82-83 0.9534734796869593 2312 84-85 1.072358068019991 2312 86-87 1.0566227352228665 2312 88-89 1.071576610869979 2312 90-91 1.157403234589335 2312 92-93 1.1047747470302447 2312 94-95 1.2024364704585686 2312 96-97 1.2135936035442754 2312 98-99 1.3242443424934933 2312 100 1.5549638048518233 2313 1 0.4924322092684541 2313 2 0.4839745103377133 2313 3 0.4786662042755232 2313 4 0.4237797429388124 2313 5 0.4254480940098162 2313 6 0.42536937428774735 2313 7 0.47111559960114846 2313 8 0.5102418503962625 2313 9 0.5394743245894205 2313 10-11 0.630953281257014 2313 12-13 0.6657257139610593 2313 14-15 0.7574355178393191 2313 16-17 0.7362346304957157 2313 18-19 0.7335887173134239 2313 20-21 0.7292957738238428 2313 22-23 0.683576265211677 2313 24-25 0.7327607296763219 2313 26-27 0.8018472785561102 2313 28-29 0.8188568957715674 2313 30-31 0.8321606900370568 2313 32-33 0.9276753932297197 2313 34-35 0.9323180089303946 2313 36-37 0.8886164131451935 2313 38-39 0.9455937348758923 2313 40-41 1.0276527075332709 2313 42-43 0.8742375110465446 2313 44-45 1.0216389055799127 2313 46-47 1.051278538059762 2313 48-49 1.1039135476817066 2313 50-51 1.0803530378723707 2313 52-53 0.9426372676898183 2313 54-55 0.8632749732591805 2313 56-57 0.8484301153635982 2313 58-59 0.8983747466089866 2313 60-61 0.8967774422070036 2313 62-63 0.8979410246295743 2313 64-65 0.8542483811330115 2313 66-67 0.8838579327896667 2313 68-69 0.8632733021327894 2313 70-71 0.8415247407377322 2313 72-73 0.8887261760543765 2313 74-75 0.857327888795453 2313 76-77 0.9001748535106628 2313 78-79 0.836633482375138 2313 80-81 0.8348448238069501 2313 82-83 0.8875502367001182 2313 84-85 0.9468130226003346 2313 86-87 0.9601984263074499 2313 88-89 0.9019311173432634 2313 90-91 0.9791803646545993 2313 92-93 0.9892428911707256 2313 94-95 1.010108329952626 2313 96-97 1.1203052282634935 2313 98-99 1.1623590994918302 2313 100 1.2448873938107212 2314 1 0.2703921560836662 2314 2 0.110810452760294 2314 3 0.20766811512156735 2314 4 0.16399597972941393 2314 5 0.09483829494440243 2314 6 0.16314626050882453 2314 7 0.2697489460506546 2314 8 0.2733843820229538 2314 9 0.4096126616132878 2314 10-11 0.39095757615996973 2314 12-13 0.43801613169824094 2314 14-15 0.5095733250727577 2314 16-17 0.5543011269093441 2314 18-19 0.4587978688000547 2314 20-21 0.49506539728687216 2314 22-23 0.5527308894333913 2314 24-25 0.556479395530296 2314 26-27 0.5813297298912516 2314 28-29 0.5164365765085464 2314 30-31 0.6484031385946665 2314 32-33 0.7126788522982608 2314 34-35 0.6151857182040388 2314 36-37 0.6027391856465059 2314 38-39 0.676532852697683 2314 40-41 0.7193860480676406 2314 42-43 0.5780615177537953 2314 44-45 0.6666516745578619 2314 46-47 0.48266103388701964 2314 48-49 0.45792928194294547 2314 50-51 0.6159287220349796 2314 52-53 0.5339277445783495 2314 54-55 0.5496268261111723 2314 56-57 0.5714429512082972 2314 58-59 0.6190363159716696 2314 60-61 0.3736704785490872 2314 62-63 0.5445058738064716 2314 64-65 0.6108643319396592 2314 66-67 0.5763190922077257 2314 68-69 0.6483699888852001 2314 70-71 0.6267789501283971 2314 72-73 0.7469390849381412 2314 74-75 0.7153119076415919 2314 76-77 0.7973408605110919 2314 78-79 0.7941846675883291 2314 80-81 0.8120059701088316 2314 82-83 0.7716984442853771 2314 84-85 0.7488154246598349 2314 86-87 0.7470683679019139 2314 88-89 0.6781081650732652 2314 90-91 0.6839443635950175 2314 92-93 0.9282719859234838 2314 94-95 0.9455915579708218 2314 96-97 1.0481386426084711 2314 98-99 1.0204279876777633 2314 100 1.0022871001113671 2315 1 0.2280904845854117 2315 2 0.2760674268931069 2315 3 0.2894921954662877 2315 4 0.14184814767396858 2315 5 0.14163990017506478 2315 6 0.1582381637058745 2315 7 0.19387765637504373 2315 8 0.17782431902479345 2315 9 0.07115857074329313 2315 10-11 0.22105339039787708 2315 12-13 0.1946406944822101 2315 14-15 0.3822347876751877 2315 16-17 0.23093640464493603 2315 18-19 0.39106487834835235 2315 20-21 0.3301035157690393 2315 22-23 0.24958228781489566 2315 24-25 0.44095642250461253 2315 26-27 0.5130581288116147 2315 28-29 0.3096019038857847 2315 30-31 0.43965453925071074 2315 32-33 0.4635175758137393 2315 34-35 0.37734903337651815 2315 36-37 0.5162004629621038 2315 38-39 0.4877894529643285 2315 40-41 0.6389706462611286 2315 42-43 0.5650947203850833 2315 44-45 0.5644569585249499 2315 46-47 0.6090640509495415 2315 48-49 0.5319895753964659 2315 50-51 0.45914854273112127 2315 52-53 0.244637143089264 2315 54-55 0.3950512125155683 2315 56-57 0.34030672972110665 2315 58-59 0.34734398666581257 2315 60-61 0.451209214475071 2315 62-63 0.498362729512543 2315 64-65 0.5278487470651854 2315 66-67 0.3753656639142946 2315 68-69 0.4543903752875238 2315 70-71 0.4147044947742913 2315 72-73 0.46308505092347474 2315 74-75 0.20807727736011117 2315 76-77 0.5137648700669857 2315 78-79 0.49060324888753826 2315 80-81 0.5087664369397054 2315 82-83 0.4787286689506267 2315 84-85 0.48187289948381107 2315 86-87 0.42467906968352764 2315 88-89 0.4128261671185136 2315 90-91 0.5890620572742513 2315 92-93 0.7074530806546555 2315 94-95 0.6286464520764738 2315 96-97 0.6784885581903488 2315 98-99 0.8160615395132425 2315 100 0.8187921020723579 2316 1 -0.02953040068453916 2316 2 0.01029379985090273 2316 3 -0.024894718458426723 2316 4 -0.14116669590709563 2316 5 -0.16542882608163012 2316 6 -0.24080721788211434 2316 7 -0.22161301423452073 2316 8 -0.1669957329943088 2316 9 -0.14345204175837978 2316 10-11 -0.0913426350440929 2316 12-13 -0.12654301688235137 2316 14-15 -0.24231147014554466 2316 16-17 -0.14513518627956046 2316 18-19 -0.02755207276052829 2316 20-21 0.05961495244784487 2316 22-23 -0.0360941101412422 2316 24-25 -0.12914685827634997 2316 26-27 -0.19709295786634584 2316 28-29 -0.1627422155097804 2316 30-31 -0.7433484553107732 2316 32-33 -0.6248600256883634 2316 34-35 -0.5821215295822384 2316 36-37 -0.3265187973356163 2316 38-39 -0.31845606378531244 2316 40-41 -0.08895436234006127 2316 42-43 -0.17618013127498955 2316 44-45 -0.2523128507462644 2316 46-47 -0.3535726261763301 2316 48-49 -0.24859755382829007 2316 50-51 -0.15095744622893648 2316 52-53 -0.45089517396836243 2316 54-55 -0.39223458730885596 2316 56-57 -0.348499409032776 2316 58-59 -0.7950005604092922 2316 60-61 -0.34205960421456894 2316 62-63 -0.3291725823617213 2316 64-65 -0.6014990035632195 2316 66-67 -0.9306981724480892 2316 68-69 -0.8843298556209547 2316 70-71 -0.7078156631323012 2316 72-73 -0.8851815603784132 2316 74-75 -0.6619820107216654 2316 76-77 -0.42487879854963495 2316 78-79 -0.3672943758580338 2316 80-81 -0.38190161714372195 2316 82-83 -0.1399482024363614 2316 84-85 -0.13576139753379834 2316 86-87 0.002745168370573481 2316 88-89 0.05852184539421401 2316 90-91 -0.6318831879096791 2316 92-93 -0.585960244257766 2316 94-95 -0.2510202573974034 2316 96-97 0.07004170675473631 2316 98-99 0.11010574571852771 2316 100 -0.05567622052472743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 183.0 1 441.0 2 275.0 3 256.0 4 235.0 5 242.0 6 281.0 7 557.0 8 1240.0 9 2517.0 10 4793.0 11 9149.0 12 14928.0 13 19138.0 14 22416.0 15 25459.0 16 28833.0 17 33809.0 18 39144.0 19 45978.0 20 53998.0 21 63869.0 22 70338.0 23 73341.0 24 74416.0 25 77161.0 26 85308.0 27 96492.0 28 111198.0 29 131294.0 30 156439.0 31 191138.0 32 238470.0 33 305382.0 34 415430.0 35 564508.0 36 816414.0 37 1144676.0 38 1008296.0 39 220815.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22466950391712 19.082778514391457 13.49062000965921 23.201931972032213 2 15.855124822638455 19.955292273101367 40.702656983791414 23.486925920468764 3 19.050081344082052 25.968264584761314 29.39211686221669 25.589537208939944 4 12.928381922308496 16.50202320097607 37.91854527970467 32.65104959701077 5 13.514465295863564 37.60962285103427 34.22479290501958 14.651118948082587 6 30.781116837889716 36.542457144883954 18.315410530193255 14.361015487033072 7 27.828997945860195 32.3792958978218 22.049095975106265 17.74261018121174 8 26.24294291763562 35.01645283244792 20.1375640957645 18.603040154151962 9 26.982376740485993 16.13820748040911 20.59754448925409 36.281871289850805 10-11 25.280059803791175 26.102687306783608 27.808982526181442 20.808270363243768 12-13 25.673111282672533 23.829667434842836 27.998845905531006 22.498375376953625 14-15 23.343275200469556 25.276741692136806 25.754318969629097 25.62566413776454 16-17 22.463587329603556 28.02797650137876 26.63235642585317 22.876079743164517 18-19 22.160626513766505 27.278435423813953 28.71667578400649 21.844262278413055 20-21 23.180717061555427 26.515122363463732 28.507332435895233 21.7968281390856 22-23 23.19141162599366 26.229031279462617 28.285985285296793 22.293571809246927 24-25 22.70410903260706 26.344444835018333 28.582555277597137 22.36889085477747 26-27 22.525648392100848 26.56889791039258 28.35622637530334 22.549227322203233 28-29 22.327309352119986 26.75397758081568 28.53082627674517 22.38788679031916 30-31 22.982574746195265 26.523621595632257 28.432613918237415 22.061189739935063 32-33 22.840684795652127 26.637271067508163 28.195825713198648 22.326218423641066 34-35 22.942120388128366 26.70767328393937 28.32419416180422 22.02601216612805 36-37 22.879143835170698 26.562878265283548 28.132093490908616 22.42588440863714 38-39 22.760102975075036 26.71026850559639 28.249924037781394 22.27970448154718 40-41 22.809265077099138 26.26380088679103 28.559949843211218 22.36698419289861 42-43 22.649661798896616 26.433026288041116 28.130255915915757 22.78705599714651 44-45 22.45904373685474 26.82094780135958 28.18716005386252 22.53284840792316 46-47 22.53109814421676 26.55912186736297 27.850531681361552 23.05924830705872 48-49 22.795659415571393 26.59169773850803 27.785113553826445 22.82752929209413 50-51 22.678794832289753 26.739027020131285 27.720211153227165 22.8619669943518 52-53 22.60342549176646 26.93102238716892 27.56670019790837 22.898851923156254 54-55 22.496473620041975 26.788951386641426 27.703670510416757 23.010904482899843 56-57 22.69699604252999 26.80607876245628 27.36688713359313 23.1300380614206 58-59 22.473322540393994 26.791669829455124 27.76000785591906 22.974999774231822 60-61 22.763035102804157 26.979131811302548 27.522385330072314 22.735447755820985 62-63 22.743430123223277 26.79424054403287 27.588006920824 22.874322411919856 64-65 22.44079510561049 26.620906921303185 27.590267520972965 23.34803045211336 66-67 22.58630602570568 26.543317699523485 27.45688227569521 23.413493999075623 68-69 22.249172678312494 26.654427767742323 27.70103424770883 23.395365306236354 70-71 22.410483946292484 26.391756871849896 27.752726658524036 23.445032523333584 72-73 22.468201127209323 26.72532429439577 27.426962059406712 23.379512518988196 74-75 22.449094818773787 26.814822805801054 27.42279764916394 23.31328472626122 76-77 22.386407430347006 26.598321007645993 27.42129362319885 23.59397793880815 78-79 22.288013104330833 26.5393129925027 27.52149502110935 23.651178882057117 80-81 22.242385389372927 26.80759204402631 27.389750947231743 23.56027161936902 82-83 22.643118311115494 26.495666833774372 27.683530876768724 23.17768397834141 84-85 22.575584952110567 26.622195126773423 27.298852702289622 23.503367218826387 86-87 22.165348962238607 26.843761064942097 27.5379071898692 23.452982782950098 88-89 22.348001641004412 26.292587961921015 27.641811243158482 23.71759915391609 90-91 22.615007413375878 26.250137009731983 27.630869621010127 23.503985955882012 92-93 22.571830312673104 26.36376057286688 27.527948759867744 23.536460354592275 94-95 22.189319089058916 26.15456754288789 27.70413611887575 23.951977249177446 96-97 22.314703684496067 26.104141614875896 27.62789809735099 23.95325660327704 98-99 22.127815932556782 26.159255578966555 27.539924167885367 24.173004320591293 100 22.453073188906238 25.985169513950417 27.265677783044744 24.2960795140986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1554.0 1 1134.5 2 1403.0 3 1910.5 4 1384.0 5 1153.0 6 1627.0 7 2811.0 8 4582.0 9 5585.5 10 5037.5 11 4387.0 12 4498.5 13 4831.5 14 5280.0 15 5802.0 16 6329.5 17 6858.0 18 7298.0 19 7846.0 20 8502.5 21 9277.5 22 10620.5 23 12283.5 24 14124.5 25 16794.0 26 20563.5 27 25225.5 28 30422.0 29 37974.5 30 48899.0 31 60935.0 32 76140.0 33 94531.0 34 113423.5 35 130869.5 36 151792.0 37 175743.0 38 188001.0 39 192166.0 40 191443.5 41 188982.5 42 191784.0 43 199372.5 44 209071.5 45 222559.0 46 234104.5 47 240599.0 48 249334.0 49 254330.5 50 252149.5 51 247196.5 52 240609.5 53 233261.5 54 223958.0 55 206011.0 56 185613.0 57 163194.0 58 140880.5 59 122264.5 60 101706.0 61 82214.0 62 65747.5 63 51613.5 64 39187.5 65 28624.5 66 20915.0 67 15826.0 68 12026.0 69 9029.5 70 6856.0 71 5113.5 72 3923.5 73 2955.5 74 2194.0 75 1659.0 76 1182.5 77 784.5 78 537.0 79 356.5 80 235.0 81 174.5 82 134.0 83 102.0 84 75.5 85 58.5 86 46.5 87 34.0 88 34.0 89 26.0 90 22.0 91 19.5 92 10.5 93 9.0 94 6.0 95 5.0 96 3.5 97 2.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024296653355548346 2 0.11188820286892412 3 0.0168645445312608 4 0.017303640676240586 5 0.01971053806353721 6 0.04587741574029577 7 0.046674293929333165 8 0.03794115949029069 9 0.032606954469795474 10-11 0.036225431960832624 12-13 0.03552613069290185 14-15 0.03133845449540941 16-17 0.037998079360936216 18-19 0.03711988707097664 20-21 0.048487598379897856 22-23 0.04822739325694687 24-25 0.06815747939297398 26-27 0.059814652638357974 28-29 0.05110591242959216 30-31 0.04927634515884303 32-33 0.0442917907723132 34-35 0.04606443817241679 36-37 0.040900992763858154 38-39 0.04630838047518334 40-41 0.056366934759257414 42-43 0.057554120632721285 44-45 0.06049769108610432 46-47 0.06135962055587946 48-49 0.060066726351216744 50-51 0.055464348239021175 52-53 0.06471789292396563 54-55 0.05161819126540191 56-57 0.05532611426745346 58-59 0.05383806622057751 60-61 0.04978862399465278 62-63 0.056659665522577264 64-65 0.054415396337125006 66-67 0.052585829066375885 68-69 0.05792816549696333 70-71 0.057911902676778884 72-73 0.06066031928794868 74-75 0.05174016241678519 76-77 0.05245572650490039 78-79 0.06262812053026551 80-81 0.06263625194035773 82-83 0.05298426816089458 84-85 0.059221059701626035 86-87 0.05625309501796635 88-89 0.06469349869368897 90-91 0.07088150177386711 92-93 0.07595550167141135 94-95 0.07912675160737649 96-97 0.07546761706587825 98-99 0.08391615215169308 100 0.1300375101947554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6148995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.71012096745061 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93134877384932 45.93580141475676 2 13.644437434289774 16.294220194685074 3 4.374644890484369 7.8363172680138415 4 1.8519475752869983 4.42320054983054 5 0.9400242075562744 2.8064479572758523 6 0.5607997707550587 2.009125329018187 7 0.36149454681738225 1.510941818367774 8 0.2365199004895581 1.1298105495552715 9 0.173386923787616 0.9317658774179421 >10 0.8406856413449836 8.775761391797827 >50 0.048343391854917615 2.002085717006261 >100 0.03366593809598814 3.8787408956810725 >500 0.001919136004429251 0.7965404315633364 >1k 6.752508309705042E-4 0.6802504015321079 >5k 3.5539517419376896E-5 0.19709775796782225 >10k+ 7.107903483875379E-5 0.791892445530278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20668 0.3361199675719366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16649 0.2707596932506857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9288 0.15104907387304753 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6262820184436645E-5 2 0.0 0.0 0.0 0.0 1.6262820184436645E-5 3 0.0 0.0 0.0 0.0 1.6262820184436645E-5 4 0.0 0.0 0.0 0.0 1.6262820184436645E-5 5 0.0 0.0 0.0 0.0 1.6262820184436645E-5 6 0.0 0.0 0.0 0.0 3.252564036887329E-5 7 0.0 0.0 0.0 0.0 3.252564036887329E-5 8 0.0 0.0 0.0 1.6262820184436645E-5 4.8788460553309934E-5 9 0.0 0.0 0.0 3.252564036887329E-5 4.8788460553309934E-5 10-11 0.0 0.0 0.0 3.252564036887329E-5 4.8788460553309934E-5 12-13 0.0 0.0 0.0 4.065705046109161E-5 4.8788460553309934E-5 14-15 0.0 8.131410092218322E-6 0.0 4.8788460553309934E-5 5.6919870645528256E-5 16-17 0.0 1.6262820184436645E-5 0.0 4.8788460553309934E-5 8.131410092218322E-5 18-19 0.0 1.6262820184436645E-5 0.0 4.8788460553309934E-5 1.3010256147549316E-4 20-21 0.0 1.6262820184436645E-5 0.0 1.057083311988382E-4 1.3823397156771146E-4 22-23 0.0 1.6262820184436645E-5 0.0 3.740448642420428E-4 1.7075961193658477E-4 24-25 0.0 2.4394230276654967E-5 0.0 9.839006211584168E-4 1.9515384221323973E-4 26-27 0.0 3.252564036887329E-5 0.0 0.002528868538679898 2.1141666239767637E-4 28-29 8.131410092218322E-6 3.252564036887329E-5 0.0 0.0063424998719302916 2.1141666239767637E-4 30-31 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.019255179098372988 2.1141666239767637E-4 32-33 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.04962599579280842 2.2767948258211302E-4 34-35 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.09695080252951904 2.2767948258211302E-4 36-37 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.17112552539073458 2.2767948258211302E-4 38-39 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.2799807122952613 2.2767948258211302E-4 40-41 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.3999352739756659 2.2767948258211302E-4 42-43 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.5141409287208722 2.4394230276654965E-4 44-45 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.6558714066282376 2.8459935322764127E-4 46-47 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.8221099545535491 3.1712499359651453E-4 48-49 1.6262820184436645E-5 3.252564036887329E-5 0.0 0.9982444285610901 3.333878137809512E-4 50-51 1.6262820184436645E-5 3.252564036887329E-5 0.0 1.1882348253657713 3.4965063396538785E-4 52-53 1.6262820184436645E-5 3.252564036887329E-5 0.0 1.372264898572856 3.6591345414982445E-4 54-55 1.6262820184436645E-5 3.252564036887329E-5 0.0 1.5764527373985504 3.740448642420428E-4 56-57 1.6262820184436645E-5 3.252564036887329E-5 0.0 1.807352258377182 3.740448642420428E-4 58-59 1.6262820184436645E-5 3.252564036887329E-5 0.0 2.0739811953010205 3.8217627433426117E-4 60-61 1.6262820184436645E-5 3.252564036887329E-5 0.0 2.3450661449553953 4.065705046109161E-4 62-63 1.6262820184436645E-5 3.252564036887329E-5 0.0 2.620102959914588 4.878846055330993E-4 64-65 1.6262820184436645E-5 3.252564036887329E-5 0.0 2.894310371044374 4.878846055330993E-4 66-67 1.6262820184436645E-5 3.252564036887329E-5 0.0 3.2141431242015974 4.878846055330993E-4 68-69 1.6262820184436645E-5 3.252564036887329E-5 0.0 3.5668267741313824 4.878846055330993E-4 70-71 1.6262820184436645E-5 3.252564036887329E-5 0.0 3.9332199814766478 5.204102459019726E-4 72-73 1.6262820184436645E-5 3.252564036887329E-5 0.0 4.289392006335994 5.773301165475008E-4 74-75 1.6262820184436645E-5 3.252564036887329E-5 0.0 4.646458486305486 5.854615266397191E-4 76-77 1.6262820184436645E-5 3.252564036887329E-5 0.0 5.038969782866956 5.935929367319375E-4 78-79 1.6262820184436645E-5 3.252564036887329E-5 0.0 5.470113408776556 6.017243468241559E-4 80-81 1.6262820184436645E-5 3.252564036887329E-5 0.0 5.9080792877535275 6.179871670085925E-4 82-83 1.6262820184436645E-5 3.252564036887329E-5 0.0 6.353306515942849 6.667756275619024E-4 84-85 2.4394230276654967E-5 3.252564036887329E-5 0.0 6.799476987702869 6.830384477463391E-4 86-87 3.252564036887329E-5 3.252564036887329E-5 0.0 7.264292782804344 7.07432678022994E-4 88 3.252564036887329E-5 3.252564036887329E-5 0.0 7.629067839541259 7.31826908299649E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11020 0.0 52.845097 1 GTATCAA 19705 0.0 40.654053 1 CAACGCA 24155 0.0 32.311043 5 ATCAACG 24240 0.0 32.237034 3 TATCAAC 24875 0.0 31.597872 2 TCAACGC 25300 0.0 30.941856 4 AACGCAG 25880 0.0 30.484695 6 TACCTGG 4595 0.0 28.15086 2 GTACATG 27650 0.0 26.879898 1 ACGCAGA 28745 0.0 26.86 7 CGCAGAG 29640 0.0 25.970728 8 TACATGG 28380 0.0 25.723127 2 ACATGGG 28545 0.0 24.922482 3 GTACTAG 2230 0.0 24.680086 1 GTGGTAT 5230 0.0 24.554249 1 TGGTATC 5300 0.0 23.341263 2 GCAGAGT 32025 0.0 23.318134 9 CATGGGG 19360 0.0 23.130203 4 GTACCTG 5995 0.0 22.441015 1 GAGTACT 16850 0.0 22.185776 12-13 >>END_MODULE