FastQCFastQC Report
Mon 6 Jun 2016
ERR1211316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184130.2994473080560319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155940.253602417956105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89580.14568234321218346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111700.049.323251
GTATCAA194500.039.690181
CAACGCA238050.031.6675825
ATCAACG239800.031.5790653
TATCAAC251000.030.3728622
TCAACGC250500.030.3248164
TACCTGG45600.030.1445522
AACGCAG255300.029.7113846
ACGCAGA286100.026.2340777
CGCAGAG294400.025.5103978
GTACATG272800.025.2533191
TACATGG280000.024.3787272
ACATGGG279300.023.5348873
GTACCTG60300.023.5031681
GTGGTAT57400.023.4642561
GCAGAGT322950.022.804529
CATGGGG187650.022.478654
ACCTGGG59700.022.476913
TGGTATC58850.022.1617662
ATGGGGG125150.021.8152075
Produced by FastQC (version 0.11.5)