##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211316_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6148995 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14001946659576 33.0 31.0 34.0 30.0 34.0 2 32.2716427643867 34.0 31.0 34.0 30.0 34.0 3 32.336015560266354 34.0 31.0 34.0 30.0 34.0 4 35.97014585310282 37.0 35.0 37.0 35.0 37.0 5 35.87676766691142 37.0 35.0 37.0 35.0 37.0 6 35.891793049108024 37.0 35.0 37.0 35.0 37.0 7 35.80905806558633 37.0 35.0 37.0 35.0 37.0 8 35.78798779312717 37.0 35.0 37.0 33.0 37.0 9 37.406560909547004 39.0 37.0 39.0 34.0 39.0 10-11 37.35482700831599 39.0 37.0 39.0 34.0 39.0 12-13 37.28826442369851 39.0 37.0 39.0 34.0 39.0 14-15 38.52974860119418 40.0 38.0 41.0 34.0 41.0 16-17 38.508649868799694 40.0 38.0 41.0 34.0 41.0 18-19 38.51270890608953 40.0 38.0 41.0 34.0 41.0 20-21 38.48111626371464 40.0 38.0 41.0 34.0 41.0 22-23 38.3760870678867 40.0 38.0 41.0 34.0 41.0 24-25 38.28971482331666 40.0 38.0 41.0 34.0 41.0 26-27 38.19416481880372 40.0 38.0 41.0 33.5 41.0 28-29 38.09355813429674 40.0 37.5 41.0 33.0 41.0 30-31 38.10135477423546 40.0 37.0 41.0 33.0 41.0 32-33 38.03889302235569 40.0 37.0 41.0 33.0 41.0 34-35 37.91379615693296 40.0 37.0 41.0 33.0 41.0 36-37 37.774012582543975 40.0 37.0 41.0 33.0 41.0 38-39 37.66778205218901 40.0 37.0 41.0 33.0 41.0 40-41 37.556058835630864 40.0 36.5 41.0 32.5 41.0 42-43 37.454657549729674 40.0 36.0 41.0 32.0 41.0 44-45 37.30339567685451 40.0 36.0 41.0 31.5 41.0 46-47 37.06952306840386 39.5 36.0 41.0 31.0 41.0 48-49 35.527478474124635 39.0 35.0 41.0 24.5 41.0 50-51 36.25490002837862 39.0 35.0 40.0 29.0 41.0 52-53 36.35075593653923 39.0 35.0 40.0 30.0 41.0 54-55 36.180870369873446 38.5 35.0 40.0 29.0 41.0 56-57 35.972153660882796 38.0 35.0 40.0 29.0 41.0 58-59 35.7345564600394 38.0 34.5 40.0 28.0 41.0 60-61 35.48020204927797 37.5 34.0 40.0 28.0 41.0 62-63 35.153970364262776 37.0 34.0 40.0 28.0 41.0 64-65 34.82009946340825 36.5 34.0 39.5 27.0 41.0 66-67 34.48224539132005 36.0 33.0 39.0 26.5 40.5 68-69 34.11981608376654 35.5 33.0 39.0 26.0 40.0 70-71 33.76228928467172 35.0 33.0 38.0 26.0 40.0 72-73 33.383043245278294 35.0 32.0 37.5 26.0 39.5 74-75 33.021236641109645 35.0 32.0 37.0 25.5 39.0 76-77 32.08338679735469 34.0 30.5 36.0 24.5 38.0 78-79 32.51539137696486 35.0 32.0 36.0 25.0 38.0 80-81 32.39602764354175 35.0 32.0 35.5 25.0 37.0 82-83 32.12570680249374 34.5 31.5 35.0 25.0 37.0 84-85 31.846608592135787 34.0 31.0 35.0 24.5 36.5 86-87 31.596542036544186 34.0 31.0 35.0 24.0 36.0 88-89 31.376994370624793 34.0 31.0 35.0 24.0 36.0 90-91 31.16236035319593 34.0 31.0 35.0 23.0 35.0 92-93 30.974385489010807 34.0 30.5 35.0 22.5 35.0 94-95 30.785885579676027 34.0 30.0 35.0 20.5 35.0 96-97 30.60379850691048 34.0 30.0 35.0 20.0 35.0 98-99 30.38902031958068 34.0 29.0 35.0 20.0 35.0 100 27.92427738191363 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.28827706915808093 1101 2 -0.3154831821831863 1101 3 -0.3264375544613536 1101 4 -0.1684911816161616 1101 5 -0.21558598560161357 1101 6 -0.21549014721961868 1101 7 -0.2478261821600185 1101 8 -0.21856876192785535 1101 9 -0.38325954839508825 1101 10-11 -0.40362584263903045 1101 12-13 -0.42828136582158294 1101 14-15 -0.5255978011054765 1101 16-17 -0.5259239936105757 1101 18-19 -0.5583754806935701 1101 20-21 -0.5959613746020551 1101 22-23 -0.5869052076004238 1101 24-25 -0.5462040726709319 1101 26-27 -0.6928482237630647 1101 28-29 -0.6210254377256135 1101 30-31 -0.6137928874264134 1101 32-33 -0.5791207606009507 1101 34-35 -0.6293484976030257 1101 36-37 -0.645248248034541 1101 38-39 -0.6025544020651878 1101 40-41 -0.603586651721443 1101 42-43 -0.6624657986109739 1101 44-45 -0.670492197495669 1101 46-47 -0.672915718484326 1101 48-49 0.5403682851750204 1101 50-51 -0.3291505021672876 1101 52-53 -0.6365372696467446 1101 54-55 -0.6281061262700263 1101 56-57 -0.6854159196752008 1101 58-59 -0.8486778664032428 1101 60-61 -0.8391969885993475 1101 62-63 -0.9034078734329469 1101 64-65 -0.8550413575665701 1101 66-67 -0.8262605428552021 1101 68-69 -0.8001112670627037 1101 70-71 -0.7720079449474611 1101 72-73 -0.8955796343451468 1101 74-75 -1.0035080053824004 1101 76-77 -0.8448020648446999 1101 78-79 -0.766591401938765 1101 80-81 -0.7642841849708688 1101 82-83 -0.7017803314730315 1101 84-85 -0.7792457521383476 1101 86-87 -0.7848635383697093 1101 88-89 -0.8733435453432463 1101 90-91 -0.8861651324890971 1101 92-93 -0.924697668314387 1101 94-95 -0.9566097196673873 1101 96-97 -1.0324905757740268 1101 98-99 -1.1584248262033334 1101 100 -1.0997590529314714 1102 1 -0.2887703431384132 1102 2 -0.25972213195567306 1102 3 -0.3092886520373739 1102 4 -0.22515592657700978 1102 5 -0.17902073550525444 1102 6 -0.29338750328173546 1102 7 -0.27141237510302574 1102 8 -0.29109589184886886 1102 9 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1.2582772213201139 2311 1 0.30661358586046106 2311 2 0.34279507944535226 2311 3 0.35366212011719256 2311 4 0.23599873686482198 2311 5 0.280815037576609 2311 6 0.34000814632620546 2311 7 0.35441611152069896 2311 8 0.18357293476665149 2311 9 0.4366913110805726 2311 10-11 0.33316439937892284 2311 12-13 0.45608960174438806 2311 14-15 0.3785180587520429 2311 16-17 0.4049512720775539 2311 18-19 0.6022205657481692 2311 20-21 0.5976136221000843 2311 22-23 0.630155961913303 2311 24-25 0.5529820885361687 2311 26-27 0.6313927115979823 2311 28-29 0.687415646212493 2311 30-31 0.5973052884876395 2311 32-33 0.6696633474060434 2311 34-35 0.7426235132754329 2311 36-37 0.7896754078538493 2311 38-39 0.8321705146453624 2311 40-41 0.8225132946202152 2311 42-43 0.8604080889207495 2311 44-45 0.8374568613013693 2311 46-47 0.8715207747399489 2311 48-49 -6.02249104468094 2311 50-51 -1.1152869877544234 2311 52-53 0.41442627442201996 2311 54-55 0.8172803724589315 2311 56-57 0.9046460056177139 2311 58-59 0.9627827636507789 2311 60-61 0.7918178532374966 2311 62-63 0.6848166009186727 2311 64-65 0.7947337807697821 2311 66-67 0.8971530275468211 2311 68-69 0.9335260888606882 2311 70-71 0.9108776859248309 2311 72-73 0.4434812802666883 2311 74-75 0.76036335693437 2311 76-77 0.8002386797862471 2311 78-79 0.7650942063196595 2311 80-81 0.8362514065751157 2311 82-83 0.8727908605454644 2311 84-85 0.8475096119597616 2311 86-87 0.8983198541266546 2311 88-89 0.9340308931534906 2311 90-91 0.9307789507616704 2311 92-93 0.9348017420389922 2311 94-95 0.963861915801072 2311 96-97 1.0733585178364677 2311 98-99 1.0841590905776428 2311 100 1.3391369441168273 2312 1 0.07747451213146661 2312 2 0.22849627358950642 2312 3 0.20230298391462043 2312 4 0.01885720551375414 2312 5 0.21333356622385935 2312 6 0.16112256997606522 2312 7 0.24678851695281878 2312 8 0.33222259836153967 2312 9 0.2000438392840067 2312 10-11 0.187793803976966 2312 12-13 0.2516715128873912 2312 14-15 0.32617217664137854 2312 16-17 0.42835432509158977 2312 18-19 0.3402640488909867 2312 20-21 0.3773895208688103 2312 22-23 0.6423617301223175 2312 24-25 0.7090709287295169 2312 26-27 0.6141099884116556 2312 28-29 0.7354897173847235 2312 30-31 0.7459710878169901 2312 32-33 0.7800833389489199 2312 34-35 0.39366502918942814 2312 36-37 0.6706676083055783 2312 38-39 0.7428677289189309 2312 40-41 0.5087583046027504 2312 42-43 0.5429120413439534 2312 44-45 0.684511079198991 2312 46-47 0.7473351718739707 2312 48-49 -4.770063822665541 2312 50-51 -0.7847673854986112 2312 52-53 0.5224857494084674 2312 54-55 0.7719729351495914 2312 56-57 0.8909809931133452 2312 58-59 0.9492931024978759 2312 60-61 0.9187596308498129 2312 62-63 0.6467158068076415 2312 64-65 0.9460565491893931 2312 66-67 0.9275334757276568 2312 68-69 0.8485994349229529 2312 70-71 0.8106093794350357 2312 72-73 0.8928112896387219 2312 74-75 0.4538382364237208 2312 76-77 0.7609414708690707 2312 78-79 0.45998233494375285 2312 80-81 0.6588856666984597 2312 82-83 0.6369723621069667 2312 84-85 0.7595693808827342 2312 86-87 0.8809582613001012 2312 88-89 0.9218426122546362 2312 90-91 0.7556034289037328 2312 92-93 0.8520242598057663 2312 94-95 1.0242142412265665 2312 96-97 1.0975524089804765 2312 98-99 1.1304135457870075 2312 100 0.6873252171973299 2313 1 -0.003972936370438163 2313 2 0.17897302990339625 2313 3 0.28202710699909517 2313 4 0.06702956819069783 2313 5 0.12313409035986211 2313 6 0.2867206225430081 2313 7 0.18903669928841538 2313 8 0.2798660217167068 2313 9 0.45846818799120115 2313 10-11 0.24909814108961115 2313 12-13 -0.04321867303595894 2313 14-15 0.14409503985812933 2313 16-17 0.04978423024645906 2313 18-19 0.38468867930400563 2313 20-21 0.37510536443250686 2313 22-23 0.45292469557897164 2313 24-25 0.2311714544222383 2313 26-27 0.35177491098293245 2313 28-29 0.33322012074435037 2313 30-31 0.5040757911472085 2313 32-33 0.44907755423960083 2313 34-35 0.3212538112704024 2313 36-37 -0.32474043684327114 2313 38-39 0.16647473147317982 2313 40-41 0.3954507510155807 2313 42-43 0.5359454828393382 2313 44-45 0.5121157072328302 2313 46-47 0.44799128256372 2313 48-49 -5.362107622594131 2313 50-51 -1.3675935401199837 2313 52-53 0.14520897068465644 2313 54-55 0.28039262205820137 2313 56-57 0.3274703557630687 2313 58-59 0.3600959965757369 2313 60-61 0.6697249977654067 2313 62-63 0.7529114744816781 2313 64-65 0.8319690392383237 2313 66-67 0.8646542831578259 2313 68-69 0.850904985684906 2313 70-71 0.8540062913764075 2313 72-73 0.6695855597738003 2313 74-75 0.7747857328339265 2313 76-77 0.76854829827289 2313 78-79 0.758167018088379 2313 80-81 -0.09849479292324759 2313 82-83 0.3657696280620044 2313 84-85 0.7086444134466454 2313 86-87 0.7770125149790346 2313 88-89 0.8130733404001091 2313 90-91 0.835563638787896 2313 92-93 0.9567534172164756 2313 94-95 0.7244101241757974 2313 96-97 0.8224348463286084 2313 98-99 0.9714542057445144 2313 100 1.0685879585796414 2314 1 0.049544897764008056 2314 2 0.17260654351977678 2314 3 0.16856065739219162 2314 4 0.01166261116801337 2314 5 0.16484094137302208 2314 6 0.26463273391215836 2314 7 0.07253038423799296 2314 8 0.24924747853749807 2314 9 0.33039006704981944 2314 10-11 0.2626103687040384 2314 12-13 0.1459570142128115 2314 14-15 0.17222601125202175 2314 16-17 0.3991617264980647 2314 18-19 0.4327568731044309 2314 20-21 0.4845823121195565 2314 22-23 0.19938847308756635 2314 24-25 0.34995831150483525 2314 26-27 0.39611592627741743 2314 28-29 0.479657665911553 2314 30-31 0.399746940458094 2314 32-33 0.4034255648251275 2314 34-35 0.2555462967333213 2314 36-37 0.33024447956042025 2314 38-39 0.3875889373147672 2314 40-41 0.22039230690204192 2314 42-43 0.1999098325730273 2314 44-45 0.4450252082268378 2314 46-47 0.5063132356901292 2314 48-49 -6.521718560966136 2314 50-51 -1.7439363402423993 2314 52-53 -0.9912281372768561 2314 54-55 -0.6081964380267237 2314 56-57 -0.23579330755769234 2314 58-59 0.2250688769639737 2314 60-61 0.5066736363840434 2314 62-63 0.37350694863124545 2314 64-65 0.5423819928713698 2314 66-67 0.6488289802643052 2314 68-69 0.5950927218081219 2314 70-71 0.3572068942059019 2314 72-73 0.6024912030705352 2314 74-75 0.6168895408954924 2314 76-77 0.5414604482190128 2314 78-79 0.4312946847216139 2314 80-81 0.48174466720487885 2314 82-83 0.3779715245059734 2314 84-85 0.4515893281015231 2314 86-87 0.5629116027622132 2314 88-89 0.47194424384708356 2314 90-91 0.6049884304079498 2314 92-93 0.6600746373804007 2314 94-95 0.7015879871658193 2314 96-97 0.7551096707390883 2314 98-99 0.8876116563724175 2314 100 -0.6550504671712574 2315 1 0.1382945484822784 2315 2 0.21346400686771716 2315 3 0.26529602087205006 2315 4 0.10938252465469844 2315 5 0.23168194371447726 2315 6 0.274281697765673 2315 7 0.34931011857670313 2315 8 0.22216916224585503 2315 9 0.4064474547497028 2315 10-11 0.22982598080131567 2315 12-13 0.3938759273271799 2315 14-15 0.23194638823412816 2315 16-17 0.3197499517340958 2315 18-19 0.4413890379131544 2315 20-21 0.3486776588060607 2315 22-23 0.40400613370259464 2315 24-25 0.4321070394552535 2315 26-27 0.5260146137972441 2315 28-29 0.5976844725366632 2315 30-31 0.588614932421045 2315 32-33 0.6477193838615776 2315 34-35 0.670018000826964 2315 36-37 0.5148637900060038 2315 38-39 0.6314380198495186 2315 40-41 0.6797572863634613 2315 42-43 0.7518839588239175 2315 44-45 0.7988225642590407 2315 46-47 0.639093321070149 2315 48-49 -4.757210013042492 2315 50-51 -0.8552752690025258 2315 52-53 0.20597718534931886 2315 54-55 0.19317292929208918 2315 56-57 0.6245870672604283 2315 58-59 0.7310974389547198 2315 60-61 0.591307617954854 2315 62-63 0.35872625222938126 2315 64-65 0.5319154403527762 2315 66-67 -0.13215698695055522 2315 68-69 0.517071701718173 2315 70-71 0.4917891580223568 2315 72-73 0.612145704135564 2315 74-75 0.7937444182761695 2315 76-77 0.7476576058738971 2315 78-79 0.7417812248837734 2315 80-81 0.5572825014302722 2315 82-83 0.5688524171179878 2315 84-85 0.6440355019321125 2315 86-87 0.6766803136162736 2315 88-89 0.6001193574752364 2315 90-91 0.6660389012657753 2315 92-93 0.7255157292636341 2315 94-95 0.44582328274773886 2315 96-97 0.5366264249096027 2315 98-99 0.45960112219036375 2315 100 0.5211300832231913 2316 1 0.17027422210449572 2316 2 0.24041564903053825 2316 3 0.13897895582847042 2316 4 -0.034848660335775605 2316 5 0.10619896171919407 2316 6 0.14493676365923847 2316 7 0.18914658522692918 2316 8 0.26485126604369924 2316 9 0.40124183520152457 2316 10-11 0.3582030029046095 2316 12-13 0.2258647492482666 2316 14-15 0.40554910825243695 2316 16-17 0.43002648257119347 2316 18-19 0.10341540122607995 2316 20-21 0.43114850343663846 2316 22-23 0.5559218995581929 2316 24-25 0.5325265873895404 2316 26-27 0.5819095520715365 2316 28-29 0.6071442571467216 2316 30-31 0.5687492964169181 2316 32-33 0.5024313848732476 2316 34-35 0.5130794440092856 2316 36-37 0.3786368758441938 2316 38-39 0.4792876386955882 2316 40-41 0.17821018164303837 2316 42-43 0.034364124473874824 2316 44-45 0.3043453320314242 2316 46-47 0.2995082510860527 2316 48-49 -5.032356024190854 2316 50-51 -1.1187837254281732 2316 52-53 0.14862096285836657 2316 54-55 0.3951431580464657 2316 56-57 0.3595722622666955 2316 58-59 0.1944717209845095 2316 60-61 0.34813962191925896 2316 62-63 0.19556188646487982 2316 64-65 0.22543484706254446 2316 66-67 0.29926375524792803 2316 68-69 -0.030130566541792803 2316 70-71 -0.31524396425632517 2316 72-73 -0.2345488070401629 2316 74-75 0.01988025828657669 2316 76-77 0.07797293287808316 2316 78-79 0.1301460607699454 2316 80-81 0.1031946946798783 2316 82-83 0.010475926719081485 2316 84-85 0.17984702915375905 2316 86-87 0.11865839094767949 2316 88-89 0.016926119122089744 2316 90-91 0.052034443545665 2316 92-93 0.16234087661631236 2316 94-95 0.39671173644636326 2316 96-97 0.516868729411037 2316 98-99 0.49406747067052237 2316 100 -0.23556864135163735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 6.0 11 12.0 12 23.0 13 120.0 14 476.0 15 1345.0 16 3269.0 17 6716.0 18 11415.0 19 17244.0 20 24567.0 21 34836.0 22 44572.0 23 48199.0 24 50069.0 25 54677.0 26 65373.0 27 79454.0 28 97813.0 29 119890.0 30 148014.0 31 183861.0 32 232402.0 33 302268.0 34 422423.0 35 593591.0 36 924252.0 37 1391229.0 38 1139045.0 39 151818.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36042787499252 19.81854875442802 14.298651617252428 23.522371753327036 2 15.538099420820695 20.32008141979839 40.40751858104729 23.734300578333624 3 18.64727791245202 25.911490562521283 29.988433880403853 25.452797644622844 4 12.904144595206782 16.886010822030226 37.82783488636706 32.38200969639593 5 13.408291217837673 37.71827254152081 34.2860195636208 14.587416677020714 6 30.890894276946295 36.845156684268474 18.014812188668383 14.249136850116848 7 27.7935870485481 32.483833940966605 21.90083451049066 17.821744499994633 8 26.299348827315068 35.422889811966755 19.866223281371038 18.411538079347146 9 26.776077955624704 16.174884576056193 20.49812464045647 36.55091282786264 10-11 25.297218619514794 26.250759279060976 27.694011554865284 20.758010546558953 12-13 25.577570368862148 23.84840049367449 28.031964919122505 22.54206421834086 14-15 23.193839116791324 25.37312437647265 25.686427938513788 25.746608568222236 16-17 22.393424141934503 28.174280046908617 26.601929068851614 22.830366742305262 18-19 22.11050295381651 27.34173264764935 28.719787334318426 21.827977064215705 20-21 23.086417444165626 26.53543102200524 28.514601584975747 21.86354994885339 22-23 23.077701192344293 26.228549487646646 28.354463399533486 22.33928592047558 24-25 22.62368000744187 26.38263696185701 28.592616709760442 22.40106632094068 26-27 22.46261946476079 26.6039018772029 28.389276676214575 22.544201981821725 28-29 22.284081157774192 26.785867767539962 28.57235556099823 22.357695513687613 30-31 22.905843681941082 26.493585105973803 28.520954594063006 22.079616618022104 32-33 22.78834463841494 26.66949937504219 28.212197777801272 22.3299582087416 34-35 22.875022518043917 26.714805466466107 28.36948216428595 22.040689851204018 36-37 22.83466609821407 26.55462012642648 28.22855958671262 22.38215418864683 38-39 22.732426912456308 26.68176825919824 28.32686424312458 22.25894058522087 40-41 22.738510511943467 26.287270806096867 28.628864670747696 22.34535401121197 42-43 22.602455780324775 26.39482444760068 28.251109954222674 22.75160981785187 44-45 22.364228814101697 26.778929590650353 28.304719087746637 22.552122507501313 46-47 22.481296089868877 26.52222060387462 27.928678526470925 23.067804779785586 48-49 22.814439869552626 26.651892704096834 27.806026232836235 22.7276411935143 50-51 22.653882629861325 26.685071940705367 27.76463999636948 22.89640543306383 52-53 22.514771460196965 26.87058940261084 27.632036318476345 22.982602818715858 54-55 22.43524117903988 26.747738581823477 27.73052120227191 23.086499036864737 56-57 22.688238716736528 26.735474301429974 27.42219802497909 23.15408895685441 58-59 22.354953912291368 26.75134756576163 27.828050251004555 23.065648270942447 60-61 22.676889206101354 27.00063208477954 27.579713794809162 22.742764914309944 62-63 22.6767252136708 26.816995689461336 27.547478582501476 22.95880051436639 64-65 22.341276728502972 26.624083349612892 27.54903346584163 23.485606456042504 66-67 22.438384599179628 26.59075954031141 27.443277066569276 23.527578793939686 68-69 22.160600064469012 26.650607164519368 27.653838016853204 23.53495475415842 70-71 22.306468448184248 26.389472363139443 27.682636568152535 23.621422620523774 72-73 22.33957672023083 26.72868139629685 27.35456813627874 23.577173747193584 74-75 22.336278476384532 26.818960879106008 27.308516908712278 23.536243735797182 76-77 22.184821162663486 26.637850591559335 27.33911910284735 23.838209142929827 78-79 22.08947198588527 26.556329767786206 27.402629466312934 23.951568780015585 80-81 22.036008116007647 26.880616103600747 27.214297145020616 23.86907863537099 82-83 22.398541531851862 26.563905288053068 27.510222882878587 23.52733029721648 84-85 22.36237278518859 26.675744647071415 27.087230596752228 23.874651970987767 86-87 21.883890011337083 26.886846892598804 27.382005553617557 23.847257542446556 88-89 22.025358341095284 26.421620793263806 27.383500964662026 24.16951990097888 90-91 22.22156614827607 26.288912843721075 27.46131427443942 24.028206733563437 92-93 22.17919129157554 26.444783534069494 27.316450616985254 24.05957455736971 94-95 21.75805765514951 26.242095875498357 27.471699089037937 24.5281473803142 96-97 21.79898257791328 26.199159491186496 27.482967950285385 24.518889980614837 98-99 21.608444504843042 26.306181370973913 27.351838889286036 24.73353523489701 100 21.610596054070903 26.205438455726892 27.327355027548883 24.85661046265332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1388.0 1 1014.5 2 1226.0 3 1620.5 4 1116.5 5 920.0 6 1378.5 7 2503.0 8 4043.5 9 4947.0 10 4545.5 11 3878.5 12 3964.0 13 4363.5 14 4837.0 15 5410.0 16 6064.0 17 6556.0 18 7124.0 19 7710.0 20 8402.0 21 9344.5 22 10464.0 23 12093.0 24 14118.5 25 16664.5 26 20409.0 27 25171.5 28 30111.0 29 37655.0 30 48882.5 31 60743.5 32 76161.0 33 95232.0 34 113620.5 35 131325.0 36 152628.5 37 175932.5 38 188559.5 39 192357.0 40 190771.5 41 188066.0 42 191293.0 43 199305.5 44 210135.0 45 224363.5 46 236170.5 47 243009.0 48 250271.0 49 254999.0 50 253856.5 51 249449.5 52 243873.5 53 237720.0 54 228373.5 55 209559.0 56 187806.5 57 164093.5 58 141440.5 59 122602.5 60 100773.5 61 80216.5 62 63389.0 63 48802.0 64 36268.5 65 26020.5 66 18764.0 67 14226.0 68 10725.0 69 7765.0 70 5781.0 71 4370.5 72 3356.5 73 2443.5 74 1803.0 75 1258.0 76 819.5 77 483.5 78 270.5 79 174.0 80 124.0 81 78.0 82 49.0 83 26.5 84 15.0 85 12.0 86 11.5 87 9.0 88 4.0 89 2.0 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028931557108112786 2 0.001984064062501271 3 1.6262820184436645E-5 4 0.021954807248989468 5 5.041474257175359E-4 6 8.131410092218322E-5 7 1.6262820184436645E-5 8 0.003935602484633668 9 0.009334858785866634 10-11 0.0011383974129105649 12-13 6.505128073774658E-5 14-15 1.057083311988382E-4 16-17 2.927307633198596E-4 18-19 1.6262820184436645E-5 20-21 8.131410092218322E-5 22-23 2.602051229509863E-4 24-25 3.252564036887329E-5 26-27 9.757692110661987E-5 28-29 9.513749807895437E-4 30-31 0.0022849262359133484 32-33 0.009432435706973254 34-35 0.01866158616164105 36-37 0.021328688671888658 38-39 0.0211254034195832 40-41 0.004854451825054338 42-43 0.005171576818650853 44-45 0.00929420173540554 46-47 0.012172720908050827 48-49 1.1943659085753038 50-51 0.018206227196476823 52-53 0.018864871413946507 54-55 0.009521881217987655 56-57 0.007358926133457581 58-59 0.00814767291240276 60-61 0.004789400544316592 62-63 0.007472765874748637 64-65 0.01958043550206172 66-67 0.029110448130141593 68-69 0.030175662852222195 70-71 0.027167041118101417 72-73 0.024020185412412923 74-75 0.009221019044575576 76-77 0.014994320210050587 78-79 0.008505454956460365 80-81 0.0025857884093254263 82-83 0.014644669576085198 84-85 0.024703223860159263 86-87 0.023914477081214084 88-89 0.03290781664320755 90-91 0.02136934572234975 92-93 0.013555060623727942 94-95 0.0062693171811003265 96-97 0.003569689030483843 98-99 0.015441547765122592 100 0.03420071084787026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6148995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.76841503213485 #Duplication Level Percentage of deduplicated Percentage of total 1 75.73082958761005 44.50580823932545 2 14.377326883581901 16.89865426694022 3 4.583458471501684 8.08087769207296 4 1.976510802153831 4.646256285458963 5 0.9749158180921185 2.864712870951545 6 0.5803399351797158 2.0463394902218264 7 0.3816938824925439 1.5702081151084106 8 0.24973948618376676 1.1741435019167767 9 0.1861645645673668 0.9846536755294507 >10 0.8718006724323765 8.879952640573118 >50 0.049912944593706175 2.0332869865855416 >100 0.03446939905002111 3.9366399127209637 >500 0.002073596684367692 0.8348613392646455 >1k 6.548193227434814E-4 0.6250444632722063 >5k 3.637885126336728E-5 0.1915155391290445 >10k+ 7.275770252673456E-5 0.7270449809289369 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18413 0.2994473080560319 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15594 0.253602417956105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8958 0.14568234321218346 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6262820184436645E-5 2 0.0 0.0 0.0 0.0 1.6262820184436645E-5 3 0.0 0.0 0.0 0.0 1.6262820184436645E-5 4 0.0 0.0 0.0 0.0 1.6262820184436645E-5 5 0.0 0.0 0.0 0.0 1.6262820184436645E-5 6 0.0 0.0 0.0 0.0 3.252564036887329E-5 7 0.0 0.0 0.0 0.0 3.252564036887329E-5 8 0.0 0.0 0.0 3.252564036887329E-5 6.505128073774658E-5 9 0.0 0.0 0.0 4.8788460553309934E-5 8.131410092218322E-5 10-11 0.0 0.0 0.0 5.6919870645528256E-5 8.131410092218322E-5 12-13 0.0 0.0 0.0 7.318269082996491E-5 9.757692110661987E-5 14-15 0.0 0.0 0.0 8.131410092218322E-5 1.3823397156771149E-4 16-17 0.0 0.0 0.0 8.944551101440154E-5 1.6262820184436645E-4 18-19 0.0 0.0 0.0 9.757692110661987E-5 1.9515384221323973E-4 20-21 0.0 0.0 0.0 1.4636538165992981E-4 2.195480724898947E-4 22-23 0.0 0.0 0.0 4.065705046109161E-4 2.683365330432046E-4 24-25 0.0 0.0 0.0 0.0010489519018961634 3.0899358350429623E-4 26-27 0.0 0.0 0.0 0.002602051229509863 3.1712499359651453E-4 28-29 0.0 0.0 0.0 0.006472602433405784 3.252564036887329E-4 30-31 0.0 0.0 0.0 0.019425938710309572 3.252564036887329E-4 32-33 0.0 0.0 0.0 0.04991059514603606 3.252564036887329E-4 34-35 0.0 0.0 0.0 0.09752000123597435 3.9030768442647947E-4 36-37 0.0 0.0 0.0 0.17128815359257896 3.9030768442647947E-4 38-39 0.0 0.0 0.0 0.27641915467486966 3.9030768442647947E-4 40-41 0.0 0.0 0.0 0.39155179017058883 3.9843909451869777E-4 42-43 0.0 0.0 0.0 0.5009029930907408 4.147019147031344E-4 44-45 0.0 0.0 0.0 0.6367300672711557 4.2283332479535273E-4 46-47 0.0 0.0 0.0 0.7959512082868827 4.472275550720077E-4 48-49 0.0 0.0 0.0 0.967068602267525 4.960160156253176E-4 50-51 0.0 0.0 0.0 1.1567093484382407 5.204102459019726E-4 52-53 0.0 0.0 0.0 1.3407882101058792 5.610672963630642E-4 54-55 0.0 0.0 0.0 1.5451224143132334 6.179871670085925E-4 56-57 0.0 0.0 0.0 1.7765342141276745 6.179871670085925E-4 58-59 0.0 0.0 0.0 2.0441307888524873 6.179871670085925E-4 60-61 0.0 0.0 0.0 2.31598009105553 6.179871670085925E-4 62-63 0.0 0.0 0.0 2.5908705406330625 6.179871670085925E-4 64-65 0.0 0.0 0.0 2.8650942145830336 6.261185771008108E-4 66-67 0.0 0.0 0.0 3.1857482401595707 6.342499871930291E-4 68-69 0.0 0.0 0.0 3.5394564477609753 6.342499871930291E-4 70-71 0.0 0.0 0.0 3.906833555727399 6.911698578385573E-4 72-73 0.0 0.0 0.0 4.263843115826244 8.050095991296138E-4 74-75 0.0 0.0 0.0 4.622300066921505 8.131410092218321E-4 76-77 0.0 0.0 0.0 5.016616536523448 8.212724193140506E-4 78-79 0.0 0.0 0.0 5.449231947659739 8.294038294062689E-4 80-81 0.0 0.0 0.0 5.888612691992757 8.375352394984872E-4 82-83 0.0 0.0 0.0 6.336011006676701 8.537980596829238E-4 84-85 0.0 0.0 0.0 6.785068129019457 8.619294697751421E-4 86-87 0.0 0.0 0.0 7.253071436877083 8.700608798673604E-4 88 0.0 0.0 0.0 7.620611173045352 8.781922899595788E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11170 0.0 49.32325 1 GTATCAA 19450 0.0 39.69018 1 CAACGCA 23805 0.0 31.667582 5 ATCAACG 23980 0.0 31.579065 3 TATCAAC 25100 0.0 30.372862 2 TCAACGC 25050 0.0 30.324816 4 TACCTGG 4560 0.0 30.144552 2 AACGCAG 25530 0.0 29.711384 6 ACGCAGA 28610 0.0 26.234077 7 CGCAGAG 29440 0.0 25.510397 8 GTACATG 27280 0.0 25.253319 1 TACATGG 28000 0.0 24.378727 2 ACATGGG 27930 0.0 23.534887 3 GTACCTG 6030 0.0 23.503168 1 GTGGTAT 5740 0.0 23.464256 1 GCAGAGT 32295 0.0 22.80452 9 CATGGGG 18765 0.0 22.47865 4 ACCTGGG 5970 0.0 22.47691 3 TGGTATC 5885 0.0 22.161766 2 ATGGGGG 12515 0.0 21.815207 5 >>END_MODULE