FastQCFastQC Report
Mon 6 Jun 2016
ERR1211315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10616508
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229820.21647419283252084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181190.1706681707393806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163250.042.1232761
GTATCAA284100.038.939831
CAACGCA332800.032.1792035
ATCAACG339200.031.5580483
TATCAAC357350.030.3648782
AACGCAG364000.029.8213236
TCAACGC361800.029.6908044
GTACTAG42800.027.913731
ACGCAGA405650.026.1580377
GTACATG421250.026.038541
CGCAGAG417500.025.3577548
TACATGG437150.024.800372
ACATGGG435750.023.9186343
GCAGAGT455200.022.5677789
TCTAACG22450.021.7888722
CTGTGCG57750.021.4828979
CTAGTAC57550.021.474483
TAGTACT59850.021.1989294
CATGGGG295600.020.8566074
GCATCGT49900.020.6228968
Produced by FastQC (version 0.11.5)