FastQCFastQC Report
Mon 6 Jun 2016
ERR1211315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10616508
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209110.19696683692980782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177450.1671453551393735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178400.043.403851
GTATCAA299350.037.483441
CAACGCA355250.030.6237585
ATCAACG364850.029.8737743
TATCAAC381450.029.0992132
AACGCAG384750.028.6900316
TCAACGC386350.028.3450494
GTACTAG40800.026.8000071
ACGCAGA431200.025.2188017
GTACATG433200.024.4176921
CGCAGAG444250.024.404078
TACATGG445600.023.2149372
ACATGGG444650.022.560293
GCAGAGT489000.021.8439759
CTAACGC21700.021.4057643
TCTAACG21300.020.4836542
TAACGCC20950.020.380494
GAGTACT223150.020.24172612-13
CATGGGG302800.019.9115264
AGAGTAC439450.019.14313910-11
Produced by FastQC (version 0.11.5)