##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211315_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10616508 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.109926823396165 33.0 31.0 34.0 30.0 34.0 2 32.24327782732326 34.0 31.0 34.0 30.0 34.0 3 32.31161564612394 34.0 31.0 34.0 30.0 34.0 4 35.949001215842344 37.0 35.0 37.0 35.0 37.0 5 35.85024209466992 37.0 35.0 37.0 35.0 37.0 6 35.86152725547798 37.0 35.0 37.0 35.0 37.0 7 35.775864060009184 37.0 35.0 37.0 33.0 37.0 8 35.75407073587662 37.0 35.0 37.0 33.0 37.0 9 37.369748979607984 39.0 37.0 39.0 34.0 39.0 10-11 37.31960678596013 39.0 37.0 39.0 34.0 39.0 12-13 37.25596066993026 39.0 37.0 39.0 33.5 39.0 14-15 38.49972024699647 40.0 38.0 41.0 34.0 41.0 16-17 38.47888185079312 40.0 38.0 41.0 34.0 41.0 18-19 38.47388237262196 40.0 38.0 41.0 34.0 41.0 20-21 38.43659068499737 40.0 38.0 41.0 34.0 41.0 22-23 38.333535094590424 40.0 38.0 41.0 34.0 41.0 24-25 38.24481774044723 40.0 38.0 41.0 33.5 41.0 26-27 38.14974198672483 40.0 38.0 41.0 33.0 41.0 28-29 38.05168276612234 40.0 37.5 41.0 33.0 41.0 30-31 38.074811840202074 40.0 37.0 41.0 33.0 41.0 32-33 38.03316608436597 40.0 37.0 41.0 33.0 41.0 34-35 37.916124350869424 40.0 37.0 41.0 33.0 41.0 36-37 37.78201994478787 40.0 37.0 41.0 33.0 41.0 38-39 37.674150907247466 40.0 37.0 41.0 33.0 41.0 40-41 37.57054866816848 40.0 37.0 41.0 32.0 41.0 42-43 37.47634448163181 40.0 36.5 41.0 32.0 41.0 44-45 37.329250399472215 40.0 36.0 41.0 31.5 41.0 46-47 37.11289639681899 39.0 36.0 41.0 31.0 41.0 48-49 35.56672038489492 39.0 35.0 41.0 25.5 41.0 50-51 36.31551400893778 39.0 35.0 40.0 29.0 41.0 52-53 36.417290082577054 39.0 35.0 40.0 30.0 41.0 54-55 36.250410257308715 38.5 35.0 40.0 29.5 41.0 56-57 36.03177687993076 38.0 35.0 40.0 29.0 41.0 58-59 35.78990417564796 38.0 34.0 40.0 28.5 41.0 60-61 35.529382307252064 37.5 34.0 40.0 28.0 41.0 62-63 35.205689337774714 37.0 34.0 40.0 28.0 41.0 64-65 34.867885231189014 36.5 34.0 39.0 28.0 41.0 66-67 34.52658896880217 36.0 33.0 39.0 27.0 40.5 68-69 34.165755632643055 35.5 33.0 39.0 26.0 40.0 70-71 33.814989495604394 35.0 33.0 38.0 26.0 40.0 72-73 33.43836480884298 35.0 32.0 37.0 26.0 39.5 74-75 33.074820270469345 35.0 32.0 37.0 26.0 39.0 76-77 32.13813638156727 34.0 30.5 36.0 25.0 38.0 78-79 32.57671675093166 35.0 32.0 36.0 26.0 38.0 80-81 32.45045951079206 35.0 32.0 35.5 25.5 37.0 82-83 32.18257373328405 34.0 31.5 35.0 25.0 37.0 84-85 31.908374062356472 34.0 31.0 35.0 25.0 36.5 86-87 31.654476170507287 34.0 31.0 35.0 24.0 36.0 88-89 31.43223463873432 34.0 31.0 35.0 24.0 36.0 90-91 31.217400109339152 34.0 31.0 35.0 23.5 35.0 92-93 31.030022960468735 34.0 30.5 35.0 23.0 35.0 94-95 30.84034675055112 34.0 30.0 35.0 22.5 35.0 96-97 30.654913225704725 34.0 30.0 35.0 20.0 35.0 98-99 30.440405687067724 34.0 29.5 35.0 20.0 35.0 100 27.965842252461922 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29775700592103505 1101 2 -0.26978300142239675 1101 3 -0.2772972783153236 1101 4 -0.19580130398835394 1101 5 -0.17426237751466545 1101 6 -0.23943855079733112 1101 7 -0.29303361082650525 1101 8 -0.250164563407246 1101 9 -0.3785993551135647 1101 10-11 -0.2794370813699274 1101 12-13 -0.38584585341075694 1101 14-15 -0.4806897525380833 1101 16-17 -0.5241376412053498 1101 18-19 -0.49863547941125574 1101 20-21 -0.5561417514903937 1101 22-23 -0.5916302961729301 1101 24-25 -0.49233258482966846 1101 26-27 -0.5884251943249055 1101 28-29 -0.6706897510758552 1101 30-31 -0.5555803171714118 1101 32-33 -0.5644702727033462 1101 34-35 -0.5911435572814412 1101 36-37 -0.6156574635560972 1101 38-39 -0.6333892984104921 1101 40-41 -0.6396460414729077 1101 42-43 -0.7423307478858661 1101 44-45 -0.6835176501645961 1101 46-47 -0.6908653779573655 1101 48-49 0.5353514376827562 1101 50-51 -0.4339297684901382 1101 52-53 -0.7841122943606038 1101 54-55 -0.6993836761071464 1101 56-57 -0.7215449047298392 1101 58-59 -0.8459036150029746 1101 60-61 -0.8387443894661644 1101 62-63 -0.8785944403039636 1101 64-65 -0.936143691553454 1101 66-67 -0.8764938976080856 1101 68-69 -0.8948737601886094 1101 70-71 -0.8913731726781648 1101 72-73 -0.9165608812820878 1101 74-75 -0.9348500143375134 1101 76-77 -0.8693850999086656 1101 78-79 -0.8388431548668649 1101 80-81 -0.7522869124095592 1101 82-83 -0.7571657788202337 1101 84-85 -0.791909631403616 1101 86-87 -0.8470284726268495 1101 88-89 -0.9255593758869018 1101 90-91 -0.9333680824843995 1101 92-93 -0.8738467421738925 1101 94-95 -0.9224411837527207 1101 96-97 -1.0667638084021043 1101 98-99 -1.1960356542664172 1101 100 -1.041029650479345 1102 1 -0.2631310169687353 1102 2 -0.27014121126327595 1102 3 -0.2888961262876073 1102 4 -0.19804858772288014 1102 5 -0.17463204651722464 1102 6 -0.20318376193371535 1102 7 -0.2153360531025328 1102 8 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1105 68-69 -0.7916638217973784 1105 70-71 -0.7884335575651775 1105 72-73 -0.7429341052559977 1105 74-75 -0.7974802189746057 1105 76-77 -0.7675212187545668 1105 78-79 -0.6832512748448067 1105 80-81 -0.6629030903437823 1105 82-83 -0.5937168408874918 1105 84-85 -0.5944584121746637 1105 86-87 -0.6177341443404103 1105 88-89 -0.6985086179064517 1105 90-91 -0.7364023591319935 1105 92-93 -0.7735848221431318 1105 94-95 -0.762976519542832 1105 96-97 -0.71979014954535 1105 98-99 -0.7085328746893502 1105 100 -0.717450482102997 1106 1 -0.2995930205201027 1106 2 -0.2801874976304788 1106 3 -0.31689063636344983 1106 4 -0.23674600185979244 1106 5 -0.22480157507438037 1106 6 -0.2921651230617144 1106 7 -0.31535378574510275 1106 8 -0.32842401786777486 1106 9 -0.4215977430636926 1106 10-11 -0.4493935802228819 1106 12-13 -0.447311772470691 1106 14-15 -0.5471678984204615 1106 16-17 -0.5562450301546207 1106 18-19 -0.5657781203631131 1106 20-21 -0.571078130404544 1106 22-23 -0.6538561099408255 1106 24-25 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2208 52-53 1.1432191836117696 2208 54-55 1.0771653897702507 2208 56-57 1.1017122031194262 2208 58-59 1.090070304517333 2208 60-61 1.131649509719061 2208 62-63 1.1341987409513692 2208 64-65 1.1712524358520469 2208 66-67 1.1289232419757198 2208 68-69 1.1037974671440338 2208 70-71 1.1328464937312432 2208 72-73 1.1898098520976106 2208 74-75 1.1385883489834967 2208 76-77 1.1355953418418565 2208 78-79 0.9941260649378165 2208 80-81 0.9400194744653447 2208 82-83 1.0006384135637276 2208 84-85 0.9910691871356399 2208 86-87 1.0407411598978165 2208 88-89 1.0538287653996825 2208 90-91 1.0327971772272804 2208 92-93 1.0955237629058523 2208 94-95 1.1675125524834122 2208 96-97 1.1609096020841783 2208 98-99 1.2401951291923474 2208 100 1.434362438093693 2209 1 0.3358229212501129 2209 2 0.39202341964329435 2209 3 0.4127253596286309 2209 4 0.26498967044032185 2209 5 0.36438155294288066 2209 6 0.40650663307609136 2209 7 0.4359999737587543 2209 8 0.4420695247799742 2209 9 0.5901088989072463 2209 10-11 0.6095104926840733 2209 12-13 0.6299320590006303 2209 14-15 0.7819341541387246 2209 16-17 0.7739842675068687 2209 18-19 0.7360414101413753 2209 20-21 0.761452612988073 2209 22-23 0.7954408117344727 2209 24-25 0.8473348968536598 2209 26-27 0.8496030513504067 2209 28-29 0.900470100134072 2209 30-31 0.8813144519188185 2209 32-33 0.8775778569219241 2209 34-35 0.9081157364496164 2209 36-37 0.9361196798036318 2209 38-39 0.9357996357700031 2209 40-41 0.9905249254915489 2209 42-43 1.0350303121334576 2209 44-45 1.053698295921329 2209 46-47 1.0503195685296447 2209 48-49 2.352899515993343 2209 50-51 1.4654397691173386 2209 52-53 1.1762597244775819 2209 54-55 1.1494465222786872 2209 56-57 1.1090669374337665 2209 58-59 1.1093188311901585 2209 60-61 1.1148041525067924 2209 62-63 1.0863956059482476 2209 64-65 1.1257259173244236 2209 66-67 1.1277942094274565 2209 68-69 1.2096924826778306 2209 70-71 1.216016750798893 2209 72-73 1.2095458205080476 2209 74-75 1.249767273800039 2209 76-77 1.1219146359227423 2209 78-79 1.0919393411993639 2209 80-81 1.0353966361568752 2209 82-83 1.03148252464851 2209 84-85 1.0724822177117694 2209 86-87 1.1017444155554195 2209 88-89 1.0979096865634297 2209 90-91 1.1721151157202456 2209 92-93 1.1063606615646044 2209 94-95 1.1177226936543008 2209 96-97 1.2279002362879936 2209 98-99 1.2580822326481105 2209 100 1.4961585159869202 2210 1 0.589194227318778 2210 2 0.5240393183574312 2210 3 0.49431968680911353 2210 4 0.34340077830337634 2210 5 0.40808184845197815 2210 6 0.4332672599715437 2210 7 0.4702470397283278 2210 8 0.4898967988934686 2210 9 0.6140007308880513 2210 10-11 0.6198959936244037 2210 12-13 0.6626705560237767 2210 14-15 0.7977601173735067 2210 16-17 0.804409920320893 2210 18-19 0.7946856689672259 2210 20-21 0.8103109762311576 2210 22-23 0.818790067017872 2210 24-25 0.8159113349102327 2210 26-27 0.8364014710636454 2210 28-29 0.8278095932610086 2210 30-31 0.8666770159388832 2210 32-33 0.827586040500428 2210 34-35 0.8633576996411847 2210 36-37 0.9281137288047532 2210 38-39 0.9350771552152537 2210 40-41 0.9636242703007056 2210 42-43 0.9264150047661275 2210 44-45 0.9821656523225997 2210 46-47 1.001221640413732 2210 48-49 2.249898416640974 2210 50-51 1.4108390990285287 2210 52-53 1.1306612611651516 2210 54-55 1.1304727447343978 2210 56-57 1.1491075025427477 2210 58-59 1.0784177934896704 2210 60-61 1.0493560424841633 2210 62-63 1.1270496638862255 2210 64-65 1.1170286153407503 2210 66-67 1.146494266518424 2210 68-69 1.15805878169175 2210 70-71 1.17727742841425 2210 72-73 1.1918167294141924 2210 74-75 1.1576325817167685 2210 76-77 1.1192356752461805 2210 78-79 1.0136190513064562 2210 80-81 1.0212055949239698 2210 82-83 1.0559626041780987 2210 84-85 1.0939040069744088 2210 86-87 1.0230243328470188 2210 88-89 1.0665305323515817 2210 90-91 1.1789268126366466 2210 92-93 1.134902181239557 2210 94-95 1.1847340906985337 2210 96-97 1.2215260991263115 2210 98-99 1.2731565711497126 2210 100 1.482859110667139 2211 1 0.5001895163605212 2211 2 0.4745514452737112 2211 3 0.44672929937033246 2211 4 0.29533965081924407 2211 5 0.34876811454327594 2211 6 0.38247261011439804 2211 7 0.4140773062895775 2211 8 0.4233441410653498 2211 9 0.5720683325023614 2211 10-11 0.5372765346607409 2211 12-13 0.5357260866568794 2211 14-15 0.6825528010232418 2211 16-17 0.6719906452501263 2211 18-19 0.6672135614685217 2211 20-21 0.6667667877380481 2211 22-23 0.6886190387798408 2211 24-25 0.7356667424219765 2211 26-27 0.7795408585620294 2211 28-29 0.7718632189183623 2211 30-31 0.7816221443284661 2211 32-33 0.7666681022270652 2211 34-35 0.8376549955669859 2211 36-37 0.8352697244987937 2211 38-39 0.8609065738960382 2211 40-41 0.8904029266862352 2211 42-43 0.7833444620798318 2211 44-45 0.8324962223135586 2211 46-47 0.9641545734595312 2211 48-49 2.1950943108408154 2211 50-51 1.3463314619517561 2211 52-53 1.108555394905558 2211 54-55 1.066834344967539 2211 56-57 1.0561853369314917 2211 58-59 1.0345276040412443 2211 60-61 1.0176731375262804 2211 62-63 1.0558589392801352 2211 64-65 1.1165346225160633 2211 66-67 1.1058580785008232 2211 68-69 1.1090221843984907 2211 70-71 1.030472363639241 2211 72-73 1.1152116951185818 2211 74-75 1.1058190127242398 2211 76-77 0.8311722965827002 2211 78-79 0.7443718424157382 2211 80-81 0.8069521420902106 2211 82-83 0.9033412248036967 2211 84-85 0.9570666001714478 2211 86-87 0.970668687197918 2211 88-89 0.9824503313823065 2211 90-91 1.0481835266108561 2211 92-93 1.090888839668171 2211 94-95 1.1014001956069421 2211 96-97 1.1228731165163168 2211 98-99 1.1855257737643612 2211 100 1.4370169149285168 2212 1 0.5212562267023202 2212 2 0.4692235036548169 2212 3 0.4555569713761045 2212 4 0.2925906039180646 2212 5 0.3233598520518868 2212 6 0.2648490566134498 2212 7 0.214613224018116 2212 8 0.3008580984763398 2212 9 0.47002644465155896 2212 10-11 0.5055735661216332 2212 12-13 0.5370286858753346 2212 14-15 0.6458990820883486 2212 16-17 0.6412821833436695 2212 18-19 0.6829745753289487 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0.9275302794935563 2212 88-89 0.9751300371587597 2212 90-91 0.9606685869203488 2212 92-93 0.9963300003033417 2212 94-95 1.0157774283406056 2212 96-97 1.0255859341542752 2212 98-99 1.0580687906201156 2212 100 1.264199733174749 2213 1 0.3987901476704181 2213 2 0.3932437274339975 2213 3 0.4347058826515635 2213 4 0.28013864492771745 2213 5 0.316327683694027 2213 6 0.33559199855872635 2213 7 0.405995907769217 2213 8 0.4100650970403166 2213 9 0.5289551408488791 2213 10-11 0.5117883983482443 2213 12-13 0.491762489102058 2213 14-15 0.6920986940398919 2213 16-17 0.6878373673264093 2213 18-19 0.6676083019199339 2213 20-21 0.536595574718504 2213 22-23 0.6529123778921075 2213 24-25 0.7017157449566938 2213 26-27 0.7720020746264211 2213 28-29 0.8018354921618638 2213 30-31 0.7582088965224756 2213 32-33 0.8005362664545501 2213 34-35 0.7414369092602442 2213 36-37 0.8296053848846796 2213 38-39 0.7714921204627885 2213 40-41 0.7809273448578509 2213 42-43 0.87343660281919 2213 44-45 0.9074210491814299 2213 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0.27518065398599134 2311 5 0.3271149465149463 2311 6 0.36277638739476714 2311 7 0.3926512626756278 2311 8 0.1516256937129583 2311 9 0.4647724803268005 2311 10-11 0.3751829390495871 2311 12-13 0.47158288536315496 2311 14-15 0.4760423901077644 2311 16-17 0.5009175700808228 2311 18-19 0.5888251775291025 2311 20-21 0.639371069022431 2311 22-23 0.6804358884884323 2311 24-25 0.6593796517493189 2311 26-27 0.6612628291575433 2311 28-29 0.7332870356737828 2311 30-31 0.5981238995086997 2311 32-33 0.6338619048483736 2311 34-35 0.7541761505065381 2311 36-37 0.819664352952266 2311 38-39 0.8179491791018378 2311 40-41 0.7948890723134738 2311 42-43 0.7922053589852638 2311 44-45 0.8281125343716766 2311 46-47 0.8621112034138818 2311 48-49 -5.908103531628811 2311 50-51 -1.0075617731183328 2311 52-53 0.3434088731351821 2311 54-55 0.7742285302144509 2311 56-57 0.9192624689455542 2311 58-59 0.897474569840945 2311 60-61 0.7750641264679814 2311 62-63 0.6632012745564779 2311 64-65 0.7312838470669831 2311 66-67 0.8996129798630008 2311 68-69 0.9397637364095388 2311 70-71 0.956253872442538 2311 72-73 0.3981505811133843 2311 74-75 0.7430435810687683 2311 76-77 0.8778094884978529 2311 78-79 0.7631221915464508 2311 80-81 0.7509334290087537 2311 82-83 0.7979324647297901 2311 84-85 0.8593608409328475 2311 86-87 0.8760862404942067 2311 88-89 0.9433356884396282 2311 90-91 1.0306092472924249 2311 92-93 0.9805240879266286 2311 94-95 0.9700555432934976 2311 96-97 0.9834523671313313 2311 98-99 1.0597703523327375 2311 100 1.2626124561449963 2312 1 0.10412799228972602 2312 2 0.23762219090993142 2312 3 0.23581898809156598 2312 4 0.01720269308701461 2312 5 0.21769205869779995 2312 6 0.1532451114470419 2312 7 0.34797332855578844 2312 8 0.3618438371882817 2312 9 0.28514143361024225 2312 10-11 0.2754407670570913 2312 12-13 0.3365992875858268 2312 14-15 0.35645897091598755 2312 16-17 0.46465226880247457 2312 18-19 0.3220768040574953 2312 20-21 0.3544054634791465 2312 22-23 0.6098999188010268 2312 24-25 0.7335103187324634 2312 26-27 0.6192754160049816 2312 28-29 0.7612566998216437 2312 30-31 0.7885182120064727 2312 32-33 0.8291851847016787 2312 34-35 0.4581260707994801 2312 36-37 0.736866235599642 2312 38-39 0.8648240840054697 2312 40-41 0.6333325859364933 2312 42-43 0.6925130718337726 2312 44-45 0.82665465261028 2312 46-47 0.9159288908395951 2312 48-49 -4.501848477935766 2312 50-51 -0.670993668681426 2312 52-53 0.5750433994009043 2312 54-55 0.8904607124143169 2312 56-57 0.9907774965637088 2312 58-59 0.9832355641773276 2312 60-61 0.9933633968233977 2312 62-63 0.8097700832499584 2312 64-65 1.0229370915596476 2312 66-67 0.9884439704563945 2312 68-69 0.8994657918606137 2312 70-71 0.967583233355775 2312 72-73 1.0316640704525923 2312 74-75 0.6906744016189776 2312 76-77 0.9164790978267305 2312 78-79 0.587424113514821 2312 80-81 0.774828919940802 2312 82-83 0.7740877416765173 2312 84-85 0.8026879799600515 2312 86-87 0.9259776974923888 2312 88-89 0.9888384341708409 2312 90-91 0.7828729078051069 2312 92-93 0.9859484394671689 2312 94-95 1.1261132312539317 2312 96-97 1.1562437837217026 2312 98-99 1.143100493144928 2312 100 0.7646357537786059 2313 1 0.04888394714657096 2313 2 0.22476635699610625 2313 3 0.28252743564422644 2313 4 0.08616877015644775 2313 5 0.14326992077932488 2313 6 0.28161562557181696 2313 7 0.14307414433650933 2313 8 0.24762294662000528 2313 9 0.4757045265275579 2313 10-11 0.23602599075156405 2313 12-13 0.06283676104602165 2313 14-15 0.1619880423441984 2313 16-17 0.11286927800182411 2313 18-19 0.45174121223016783 2313 20-21 0.42334481280903447 2313 22-23 0.5244187610221687 2313 24-25 0.31974288097051584 2313 26-27 0.42715804238959976 2313 28-29 0.47502270723190776 2313 30-31 0.6310309316959604 2313 32-33 0.526961687343551 2313 34-35 0.4161053920902944 2313 36-37 -0.29207847076272486 2313 38-39 0.24351313849498268 2313 40-41 0.4314211662591987 2313 42-43 0.5232908521868822 2313 44-45 0.5537101932706818 2313 46-47 0.44016690491008603 2313 48-49 -5.441631663765392 2313 50-51 -1.3157977230289433 2313 52-53 0.16186600781112048 2313 54-55 0.3157531044885431 2313 56-57 0.30569165875765947 2313 58-59 0.4529521217196404 2313 60-61 0.719441965519529 2313 62-63 0.8229884643340668 2313 64-65 0.8824266502011398 2313 66-67 0.9329625078142598 2313 68-69 0.9500074514277301 2313 70-71 0.9068616459869716 2313 72-73 0.6951060249018397 2313 74-75 0.8115306362400858 2313 76-77 0.8209955011416312 2313 78-79 0.7473013601066185 2313 80-81 -0.14692854586857607 2313 82-83 0.3127537758562866 2313 84-85 0.6631421124444437 2313 86-87 0.8491385001347602 2313 88-89 0.8253030892410074 2313 90-91 0.8305354886773983 2313 92-93 0.8687364433894693 2313 94-95 0.8470006151761922 2313 96-97 0.8290768322375577 2313 98-99 1.0947095813698517 2313 100 1.1935959799788058 2314 1 0.04345346116620874 2314 2 0.15585414173614964 2314 3 0.23013280998598162 2314 4 0.058809191220589696 2314 5 0.16236650079446946 2314 6 0.29700091623825386 2314 7 0.11441021798695061 2314 8 0.2623183706970096 2314 9 0.44575080696583314 2314 10-11 0.33739716124056685 2314 12-13 0.2364427216830478 2314 14-15 0.26799141591968123 2314 16-17 0.47396020378337056 2314 18-19 0.49677358446580655 2314 20-21 0.5882474891004676 2314 22-23 0.33095193893636576 2314 24-25 0.41544893906109337 2314 26-27 0.5043048306958156 2314 28-29 0.6407694182643411 2314 30-31 0.5224722959902763 2314 32-33 0.4919914145725599 2314 34-35 0.3474224679895741 2314 36-37 0.4631792842106037 2314 38-39 0.49998810473000077 2314 40-41 0.300128710736864 2314 42-43 0.38502556266170274 2314 44-45 0.5927826680417354 2314 46-47 0.6612096995433916 2314 48-49 -6.394429600383361 2314 50-51 -1.543386697586179 2314 52-53 -0.7065372474696474 2314 54-55 -0.25214793342662034 2314 56-57 0.02469532708295219 2314 58-59 0.4529441556815499 2314 60-61 0.6360261904210773 2314 62-63 0.5456499188149593 2314 64-65 0.7137028391121376 2314 66-67 0.8225550943769591 2314 68-69 0.8344184346242187 2314 70-71 0.5273681596298232 2314 72-73 0.7331297063501481 2314 74-75 0.7115819643138579 2314 76-77 0.6775666936561322 2314 78-79 0.47444751779485017 2314 80-81 0.5630446890124716 2314 82-83 0.606923861318343 2314 84-85 0.6446655407739676 2314 86-87 0.672014005199518 2314 88-89 0.5941801994592382 2314 90-91 0.792065273302903 2314 92-93 0.8058043483078627 2314 94-95 0.9387110130207716 2314 96-97 0.9381287342506006 2314 98-99 0.9866921363744829 2314 100 -0.4386757246487534 2315 1 0.15901325078625916 2315 2 0.25016795405077374 2315 3 0.3094734590409587 2315 4 0.1404115954085583 2315 5 0.2376235119344372 2315 6 0.30809839807546524 2315 7 0.3520669873778246 2315 8 0.2709711612770107 2315 9 0.44111526769510334 2315 10-11 0.22722790098863044 2315 12-13 0.4258716154101947 2315 14-15 0.2744684665189183 2315 16-17 0.3751682792496851 2315 18-19 0.4601580855134344 2315 20-21 0.40281347239020704 2315 22-23 0.46258937935640887 2315 24-25 0.5180281249259551 2315 26-27 0.5990968014763425 2315 28-29 0.6445712056844712 2315 30-31 0.6239998451005775 2315 32-33 0.6647949422811692 2315 34-35 0.704229427353269 2315 36-37 0.6061118165783554 2315 38-39 0.6681003519540312 2315 40-41 0.6711218910113885 2315 42-43 0.7122353971544442 2315 44-45 0.7489102809999579 2315 46-47 0.5997576846809736 2315 48-49 -5.08397494395906 2315 50-51 -0.9343965937302343 2315 52-53 0.2610209513153805 2315 54-55 0.21426648535832982 2315 56-57 0.5560263229698776 2315 58-59 0.7095636603363857 2315 60-61 0.5854467505843601 2315 62-63 0.3219031688746483 2315 64-65 0.5108612949761522 2315 66-67 -0.15046394930636353 2315 68-69 0.5893340946342107 2315 70-71 0.535924823382139 2315 72-73 0.6643738659332072 2315 74-75 0.8392151233765688 2315 76-77 0.7975414176183264 2315 78-79 0.7236306487448445 2315 80-81 0.578276884782639 2315 82-83 0.523265208538696 2315 84-85 0.5744905194712864 2315 86-87 0.6296115027460054 2315 88-89 0.5809276543296242 2315 90-91 0.7123797116218675 2315 92-93 0.7371946561643661 2315 94-95 0.30727687997318753 2315 96-97 0.47507286747835664 2315 98-99 0.4973660177810757 2315 100 0.45375973768707567 2316 1 0.23514138062696333 2316 2 0.2387402903624789 2316 3 0.20648632870939565 2316 4 -0.030925374569747532 2316 5 0.16179785524174406 2316 6 0.12150274624436719 2316 7 0.2322747199742068 2316 8 0.29435890468581505 2316 9 0.4501812699416803 2316 10-11 0.3788667601978304 2316 12-13 0.29276185878432415 2316 14-15 0.4788185258746793 2316 16-17 0.4580965424164347 2316 18-19 0.11027502780338949 2316 20-21 0.4703881576029332 2316 22-23 0.5763338139442808 2316 24-25 0.6551231445608678 2316 26-27 0.6143181654620591 2316 28-29 0.6607497196861161 2316 30-31 0.6385377606468978 2316 32-33 0.649184043948047 2316 34-35 0.6013908411457365 2316 36-37 0.4518417287290717 2316 38-39 0.5764677999683983 2316 40-41 0.14984239335569072 2316 42-43 0.1402205365404754 2316 44-45 0.4060510213703594 2316 46-47 0.47485819377477156 2316 48-49 -5.0149194814419005 2316 50-51 -0.923398755435791 2316 52-53 0.3731221364693411 2316 54-55 0.6569333694371409 2316 56-57 0.5387929874583364 2316 58-59 0.39436503989671934 2316 60-61 0.5634986352948772 2316 62-63 0.44922290115017205 2316 64-65 0.48554130080562885 2316 66-67 0.5396732025641597 2316 68-69 0.17974357250729156 2316 70-71 -0.13985842148124306 2316 72-73 0.043887708871260145 2316 74-75 0.15297912735439922 2316 76-77 0.21396015238963173 2316 78-79 0.3554236346370345 2316 80-81 0.28586405058563 2316 82-83 0.23169552589802578 2316 84-85 0.3482611299046958 2316 86-87 0.412341393599565 2316 88-89 0.2847077979500092 2316 90-91 0.3216433735118116 2316 92-93 0.3875121827874288 2316 94-95 0.5487477854289295 2316 96-97 0.6013001477464606 2316 98-99 0.6364344054617668 2316 100 -0.180936395152127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 6.0 11 16.0 12 50.0 13 205.0 14 761.0 15 2220.0 16 5218.0 17 10249.0 18 17452.0 19 26382.0 20 38119.0 21 54348.0 22 68197.0 23 73384.0 24 79580.0 25 92337.0 26 111894.0 27 139439.0 28 172924.0 29 212319.0 30 262753.0 31 326112.0 32 410003.0 33 534136.0 34 741880.0 35 1043552.0 36 1630448.0 37 2400912.0 38 1914315.0 39 247273.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.096716384834906 20.14416836640089 14.081940003527608 23.677175245236597 2 15.457035964890963 20.388368902229384 40.07493949397426 24.07965563890539 3 18.613998833325375 25.544240149224333 29.81881315111507 26.022947866335222 4 12.900848589545271 16.806305062434753 38.23918540830346 32.053660939716515 5 13.585489766260894 37.838526468164694 34.57550601651098 14.000477749063434 6 31.35839550933109 37.15689620207332 17.596048340965005 13.888659947630591 7 28.147916852109052 32.1378551604318 21.8340875834373 17.88014040402185 8 26.38473350724551 35.541413285461324 19.634138231359465 18.43971497593369 9 26.374610223966314 15.961917935947909 20.408574681799287 37.2548971582865 10-11 25.213503551229856 26.23270569546105 27.72684789112686 20.826942862182236 12-13 25.63470891373292 23.600088371869806 28.146774918910427 22.618427795486845 14-15 23.158516708337668 25.25656299404248 25.421207712977996 26.163712584641864 16-17 22.564980227202437 28.25725797332345 26.327701193225117 22.85006060624899 18-19 22.27248776574892 27.60397818265256 28.233191786450266 21.890342265148252 20-21 23.181209930597195 26.640456839760258 28.089615962471253 22.088717267171297 22-23 23.281617191537798 26.390576386012725 27.791667086611294 22.536139335838183 24-25 22.908828829982905 26.605658190837417 28.01127169652757 22.474241282652113 26-27 22.80103248721483 26.782646200849914 27.796440813659906 22.61988049827535 28-29 22.575244204744 26.82493847490095 28.02575053933071 22.57406678102434 30-31 23.098721313240414 26.64106187843186 27.8889609116445 22.37125589668323 32-33 23.002875505450742 26.89007844642634 27.582710228650964 22.52433581947195 34-35 23.09814098652877 26.82905115006183 27.862064086087614 22.210743777321788 36-37 23.07037929700077 26.54742195852155 27.755588388597186 22.626610355880494 38-39 22.96939449369109 26.665304041508847 27.9532062147578 22.412095250042267 40-41 22.909723435994827 26.30564292755877 28.193820170873124 22.59081346557328 42-43 22.815123351137352 26.379824177836493 27.8388122254486 22.966240245577552 44-45 22.72233805630879 26.750911884332357 27.798915333245382 22.72783472611347 46-47 22.842446283420813 26.562096539821887 27.511527097215737 23.083930079541563 48-49 23.15713893565398 26.617598838443822 27.43361245553132 22.791649770370878 50-51 22.933439350781306 26.600146684685868 27.388932513268276 23.077481451264557 52-53 22.74334272661655 26.820308390423875 27.28141350423541 23.15493537872417 54-55 22.680423248025885 26.740180066459267 27.319628562883135 23.259768122631712 56-57 22.99926448534321 26.623944393283306 27.051537647649294 23.325253473724192 58-59 22.61779301790798 26.638639903316662 27.463282756136405 23.280284322638956 60-61 22.82081112488332 26.90971554171772 27.200974417306806 23.06849891609215 62-63 22.825015022536867 26.73128437768264 27.10779686383878 23.335903735941717 64-65 22.46991617241584 26.583995209179395 27.2269244268588 23.71916419154596 66-67 22.669839203865084 26.515900641237938 27.118267823709648 23.695992331187334 68-69 22.387660036010296 26.64364755211442 27.203659050836286 23.765033361039 70-71 22.512711787555155 26.36091324656946 27.240052865772896 23.886322100102486 72-73 22.659385993255253 26.61264637633383 26.998936738158 23.729030892252915 74-75 22.645765763050033 26.762137216463866 26.931714880801543 23.660382139684554 76-77 22.52137842155428 26.635587909242304 26.89984213328962 23.943191535913794 78-79 22.492390349797812 26.55541250132764 26.901769630778006 24.050427518096544 80-81 22.429915785531357 26.905398979750622 26.78158852654634 23.883096708171678 82-83 22.75513336920079 26.517250530310534 27.13324164553071 23.59437445495797 84-85 22.794935383163594 26.612617995634423 26.680848942976187 23.91159767822579 86-87 22.302138074751472 26.901299287267587 26.928357859796932 23.86820477818401 88-89 22.473867177225475 26.422652747754828 26.918466987466676 24.185013087553017 90-91 22.563282722731604 26.347056346850962 26.94867806304149 24.140982867375943 92-93 22.656474621566534 26.484214526584644 26.73275254929875 24.126558302550073 94-95 22.216538344532132 26.321086693777524 26.99462070509735 24.467754256592993 96-97 22.3706200392723 26.26481071169599 26.900451708691698 24.464117540340013 98-99 22.146633569821464 26.401319125769234 26.851652708468816 24.600394595940482 100 22.24720324984314 26.209874113146558 26.662481839391774 24.88044079761853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1152.0 1 826.0 2 646.0 3 790.0 4 627.0 5 571.0 6 943.5 7 1812.0 8 3055.5 9 3868.0 10 3711.5 11 3374.5 12 3578.5 13 4065.5 14 4742.5 15 5528.0 16 6280.5 17 7034.0 18 7980.0 19 8783.0 20 9543.5 21 10676.0 22 12211.5 23 14233.5 24 16766.5 25 20368.5 26 25818.5 27 33313.0 28 42804.0 29 58154.0 30 79085.0 31 100511.0 32 128665.5 33 161307.5 34 193400.0 35 225177.0 36 262349.5 37 303463.0 38 324458.0 39 330065.0 40 328995.5 41 325264.0 42 331821.0 43 349439.0 44 370519.0 45 399835.0 46 426783.5 47 440171.0 48 454474.5 49 460571.0 50 451935.0 51 443338.0 52 432308.0 53 417359.5 54 400489.0 55 367415.0 56 327799.0 57 283971.0 58 241817.0 59 211345.0 60 175374.0 61 137965.5 62 108776.5 63 84949.5 64 62752.0 65 43581.5 66 31122.0 67 22993.0 68 16878.5 69 12214.5 70 9080.5 71 6760.0 72 5156.5 73 3949.5 74 2830.0 75 1925.5 76 1314.0 77 799.0 78 471.0 79 263.5 80 166.5 81 137.5 82 94.5 83 47.5 84 28.5 85 22.0 86 14.0 87 9.0 88 6.0 89 4.5 90 3.5 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029049099760486218 2 0.001978051540110929 3 8.477363743332553E-5 4 0.02173030906207578 5 5.274804106962478E-4 6 4.709646524073641E-5 7 2.8257879144441844E-5 8 0.003974941666318153 9 0.010521350334780515 10-11 0.0010879283470610112 12-13 7.064469786110462E-5 14-15 1.1303151657776737E-4 16-17 2.684498518721975E-4 18-19 1.8838586096294562E-5 20-21 6.122540481295732E-5 22-23 2.7315949839627117E-4 24-25 4.709646524073641E-5 26-27 8.006399090925189E-5 28-29 0.0010267029422480537 30-31 0.002491403011234956 32-33 0.009259165066328779 34-35 0.018146268057255735 36-37 0.020727154352448093 38-39 0.020651800008062916 40-41 0.004733194756694009 42-43 0.005218288348673594 44-45 0.009320390471141735 46-47 0.012273338841735907 48-49 1.210463930324359 50-51 0.01792491467062428 52-53 0.018918650087203816 54-55 0.009259165066328779 56-57 0.007648465955095593 58-59 0.007737949239052991 60-61 0.004921580617656955 62-63 0.0074506608010844995 64-65 0.019238906050840825 66-67 0.02949180653374914 68-69 0.0297131599203806 70-71 0.026708405438021617 72-73 0.023279782768496005 74-75 0.009588840323013933 76-77 0.014510420940670886 78-79 0.008246591063652945 80-81 0.0026374020534812386 82-83 0.014138358865269068 84-85 0.02407571303106445 86-87 0.02351526509469969 88-89 0.03354681219097654 90-91 0.02069418682677958 92-93 0.013488427644946907 94-95 0.006701827003756791 96-97 0.0035228156000070834 98-99 0.015061449583987502 100 0.03357977971664505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0616508E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.575315425190716 #Duplication Level Percentage of deduplicated Percentage of total 1 70.31976887063102 36.26764260129301 2 15.782532859518014 16.279782208761574 3 6.0916201927935045 9.425316986813582 4 2.7896167674008274 5.7550145877639425 5 1.4884530246459906 3.8383717120848067 6 0.859503306223932 2.6597492476496147 7 0.5676978836103835 2.0495438189393114 8 0.4126132778231613 1.7024527961881115 9 0.29555823202092296 1.3719158138691725 >10 1.279114401598663 11.345869941556202 >50 0.06742635693432145 2.367974966670049 >100 0.04134862518075443 4.099443332748435 >500 0.0033738821074992867 1.1476915541294523 >1k 0.001286549542200918 0.9716908801440114 >5k 0.0 0.0 >10k+ 8.576996885097953E-5 0.717539551388834 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20911 0.19696683692980782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17745 0.1671453551393735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.419293048147281E-6 0.0 0.0 0.0 6 1.1303151657776737E-4 9.419293048147281E-6 0.0 0.0 9.419293048147281E-6 7 1.1303151657776737E-4 9.419293048147281E-6 0.0 0.0 9.419293048147281E-6 8 1.1303151657776737E-4 9.419293048147281E-6 0.0 0.0 9.419293048147281E-6 9 1.1303151657776737E-4 9.419293048147281E-6 0.0 1.8838586096294562E-5 1.8838586096294562E-5 10-11 1.1303151657776737E-4 9.419293048147281E-6 0.0 1.8838586096294562E-5 1.8838586096294562E-5 12-13 1.1303151657776737E-4 9.419293048147281E-6 0.0 1.8838586096294562E-5 1.8838586096294562E-5 14-15 1.1303151657776737E-4 9.419293048147281E-6 0.0 2.8257879144441844E-5 4.238681871666277E-5 16-17 1.6954727486665107E-4 9.419293048147281E-6 0.0 3.296752566851548E-5 5.651575828888369E-5 18-19 1.6954727486665107E-4 9.419293048147281E-6 0.0 4.709646524073641E-5 7.535434438517825E-5 20-21 1.6954727486665107E-4 9.419293048147281E-6 0.0 1.3657974919813559E-4 8.477363743332553E-5 22-23 1.742569213907247E-4 1.4128939572220922E-5 0.0 5.698672294129105E-4 1.4599904224628287E-4 24-25 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.0015023772411794914 2.1193409358331384E-4 26-27 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.002952948370594173 2.4490161925182934E-4 28-29 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.008081753435310368 2.4490161925182934E-4 30-31 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.02392029469577002 2.49611265775903E-4 32-33 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.058178263511881684 2.9199808449256574E-4 34-35 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.11099223963284349 3.2025596363700757E-4 36-37 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.1933074415806026 3.3909454973330214E-4 38-39 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.3071254691278903 3.53223489305523E-4 40-41 1.7896656791479835E-4 1.8838586096294562E-5 0.0 0.42458405343828687 3.6264278235367033E-4 42-43 1.9780515401109292E-4 1.8838586096294562E-5 0.0 0.5437946262556388 3.7206207540181765E-4 44-45 2.072244470592402E-4 1.8838586096294562E-5 0.0 0.6923933933832104 3.8619101497403853E-4 46-47 2.1193409358331384E-4 1.8838586096294562E-5 0.0 0.8700836470899848 4.1915854064255404E-4 48-49 2.1664374010738746E-4 1.8838586096294562E-5 0.0 1.0618698728433116 4.3328748021477493E-4 50-51 2.3548232620368203E-4 1.8838586096294562E-5 0.0 1.262585588406282 4.3799712673884856E-4 52-53 2.3548232620368203E-4 2.3548232620368205E-5 0.0 1.4566654120168328 5.039321780758796E-4 54-55 2.543209122999766E-4 6.122540481295732E-5 0.0 1.6758759094798403 5.651575828888369E-4 56-57 2.7315949839627117E-4 6.593505133703096E-5 0.0 1.925854527684621 5.792865224610577E-4 58-59 2.8257879144441843E-4 6.593505133703096E-5 0.0 2.2075149380568453 5.981251085573524E-4 60-61 2.9199808449256574E-4 6.593505133703096E-5 0.0 2.4954015011338946 6.02834755081426E-4 62-63 2.9199808449256574E-4 6.593505133703096E-5 0.0 2.7849317308478456 6.405119272740151E-4 64-65 2.9670773101663937E-4 6.593505133703096E-5 0.0 3.082727390211546 6.405119272740151E-4 66-67 3.296752566851549E-4 6.593505133703096E-5 0.0 3.4155863679469745 6.452215737980888E-4 68-69 3.296752566851549E-4 6.593505133703096E-5 0.0 3.7809089391728428 6.499312203221624E-4 70-71 3.296752566851549E-4 7.064469786110461E-5 0.0 4.169963419233518 6.68769806418457E-4 72-73 3.3909454973330214E-4 7.535434438517825E-5 0.0 4.55819371115248 7.252855647073407E-4 74-75 3.53223489305523E-4 7.535434438517825E-5 0.0 4.943979696525449 7.347048577554879E-4 76-77 3.579331358295967E-4 8.006399090925189E-5 0.0 5.361268507497946 7.347048577554879E-4 78-79 3.579331358295967E-4 8.477363743332553E-5 0.0 5.815094756204205 7.347048577554879E-4 80-81 3.579331358295967E-4 8.477363743332553E-5 0.0 6.2932322002677346 7.676723834240034E-4 82-83 3.579331358295967E-4 8.477363743332553E-5 0.0 6.778504758815234 8.430267278091817E-4 84-85 3.767717219258913E-4 8.477363743332553E-5 0.0 7.2517206222611055 8.477363743332553E-4 86-87 3.767717219258913E-4 8.477363743332553E-5 0.0 7.736380926760475 8.571556673814026E-4 88 3.767717219258913E-4 8.477363743332553E-5 0.0 8.124592380093342 8.571556673814026E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17840 0.0 43.40385 1 GTATCAA 29935 0.0 37.48344 1 CAACGCA 35525 0.0 30.623758 5 ATCAACG 36485 0.0 29.873774 3 TATCAAC 38145 0.0 29.099213 2 AACGCAG 38475 0.0 28.690031 6 TCAACGC 38635 0.0 28.345049 4 GTACTAG 4080 0.0 26.800007 1 ACGCAGA 43120 0.0 25.218801 7 GTACATG 43320 0.0 24.417692 1 CGCAGAG 44425 0.0 24.40407 8 TACATGG 44560 0.0 23.214937 2 ACATGGG 44465 0.0 22.56029 3 GCAGAGT 48900 0.0 21.843975 9 CTAACGC 2170 0.0 21.405764 3 TCTAACG 2130 0.0 20.483654 2 TAACGCC 2095 0.0 20.38049 4 GAGTACT 22315 0.0 20.241726 12-13 CATGGGG 30280 0.0 19.911526 4 AGAGTAC 43945 0.0 19.143139 10-11 >>END_MODULE