FastQCFastQC Report
Mon 6 Jun 2016
ERR1211314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10439440
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT465810.4462020951315396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350250.3355065022644893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205690.1970316415439909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232750.051.406921
GTATCAA445250.042.7910081
ATCAACG526050.034.962183
CAACGCA524550.034.954165
TATCAAC563100.033.390772
AACGCAG571050.032.445256
GTACTAG69650.031.8724211
TCAACGC586750.031.465284
GCATCGT66550.030.1495198
ACGCAGA627950.029.0872447
CGCAGAG631400.028.8381738
TAGGCAT85750.028.440145
GCAGAGT669050.026.5925569
GTACATG614550.026.3036791
GCTATTG109050.025.681558
TACATGG640000.025.0137462
CTGTGCG86000.024.91759
CTAGGCA96100.024.7906114
ACATGGG631150.024.6210383
ATTTAGG118400.024.3502241
Produced by FastQC (version 0.11.5)