##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211314_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10439440 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.292916765650265 31.0 31.0 34.0 28.0 34.0 2 31.319500662870805 31.0 31.0 34.0 28.0 34.0 3 31.358599216049903 31.0 31.0 34.0 28.0 34.0 4 34.97326686105768 37.0 35.0 37.0 32.0 37.0 5 35.032581057987784 37.0 35.0 37.0 32.0 37.0 6 35.09910320860123 37.0 35.0 37.0 32.0 37.0 7 35.02947763481566 37.0 35.0 37.0 32.0 37.0 8 35.03485234840183 37.0 35.0 37.0 32.0 37.0 9 36.55880095100887 39.0 37.0 39.0 32.0 39.0 10-11 36.49130839393684 39.0 37.0 39.0 32.0 39.0 12-13 36.40474829109607 39.0 37.0 39.0 32.0 39.0 14-15 37.48223578084648 40.0 37.0 41.0 32.0 41.0 16-17 37.496417480247985 40.0 37.0 41.0 32.0 41.0 18-19 37.534534706842514 40.0 37.0 41.0 32.0 41.0 20-21 37.51675089851563 40.0 37.0 41.0 31.0 41.0 22-23 37.389237353727786 40.0 37.0 41.0 31.0 41.0 24-25 37.26835304384143 40.0 36.5 41.0 31.0 41.0 26-27 37.1123287264451 40.0 36.0 41.0 30.5 41.0 28-29 36.991529670173875 39.5 36.0 41.0 30.0 41.0 30-31 36.80926223054111 39.0 36.0 41.0 30.0 41.0 32-33 36.68111167840421 39.0 35.0 41.0 30.0 41.0 34-35 36.541014747917515 39.0 35.0 41.0 30.0 41.0 36-37 36.446621801552574 39.0 35.0 41.0 29.5 41.0 38-39 36.29162934027113 39.0 35.0 40.0 29.0 41.0 40-41 36.12521509774471 39.0 35.0 40.0 28.0 41.0 42-43 35.9712755186102 39.0 35.0 40.0 28.0 41.0 44-45 35.79281426973094 38.0 35.0 40.0 27.0 41.0 46-47 35.5830555566199 38.0 34.0 40.0 26.0 41.0 48-49 35.31132412274988 38.0 34.0 40.0 25.5 41.0 50-51 34.633760192117585 37.5 33.5 39.5 24.0 40.5 52-53 34.87540169779222 38.0 33.5 40.0 25.0 40.5 54-55 35.2392720778126 38.0 34.0 40.0 26.0 41.0 56-57 35.12963046868414 38.0 34.0 40.0 25.5 41.0 58-59 34.89821125462669 38.0 34.0 40.0 25.0 41.0 60-61 34.65563057022216 37.5 33.0 40.0 25.0 41.0 62-63 34.39318450031803 37.0 33.0 40.0 24.0 41.0 64-65 34.134587774823174 36.5 33.0 40.0 23.5 41.0 66-67 33.93881348041657 36.0 33.0 39.5 23.0 41.0 68-69 33.62383873081315 35.5 32.5 39.0 23.0 41.0 70-71 33.329143565172075 35.0 32.0 39.0 22.5 41.0 72-73 32.99996312062716 35.0 32.0 38.5 22.0 40.0 74-75 32.63328789666879 35.0 31.5 37.5 21.5 40.0 76-77 32.29218171664381 35.0 31.0 37.0 20.5 39.0 78-79 31.92692088847678 35.0 31.0 36.5 20.0 39.0 80-81 31.597529896239646 35.0 31.0 36.0 20.0 38.5 82-83 31.271180638041887 34.5 30.0 35.5 19.5 37.0 84-85 30.95671654801407 34.0 30.0 35.0 19.0 37.0 86-87 30.702482317059154 34.0 30.0 35.0 18.0 36.5 88-89 30.44896651544527 34.0 29.0 35.0 18.0 36.0 90-91 30.175918152697847 34.0 29.0 35.0 17.5 36.0 92-93 29.92440351206578 34.0 29.0 35.0 16.5 35.5 94-95 29.710698370793835 34.0 29.0 35.0 15.0 35.0 96-97 29.514472184331726 34.0 28.5 35.0 14.5 35.0 98-99 29.279876123623488 34.0 27.0 35.0 12.0 35.0 100 27.56109321955967 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.298463179042173 1101 2 -0.32090023766831166 1101 3 -0.35445809849742815 1101 4 -0.25692751896981747 1101 5 -0.30717000183582854 1101 6 -0.27477593185006555 1101 7 -0.3430309844002508 1101 8 -0.37812202266452744 1101 9 -0.42815669747830043 1101 10-11 -0.460817414812432 1101 12-13 -0.40516458467735106 1101 14-15 -0.46843927236771776 1101 16-17 -0.4744488541446401 1101 18-19 -0.5080861208879455 1101 20-21 -0.6233824881240295 1101 22-23 -0.5661473503584773 1101 24-25 -0.5536883336462566 1101 26-27 -0.626548806258306 1101 28-29 -0.662708333584952 1101 30-31 -0.6729619996784351 1101 32-33 -0.766329959324267 1101 34-35 -0.7608462250097929 1101 36-37 -0.8184010944061484 1101 38-39 -0.8224851709188741 1101 40-41 -0.8667939868680321 1101 42-43 -0.8188750008963623 1101 44-45 -0.9898647749941176 1101 46-47 -0.8446279056441455 1101 48-49 -0.821842036284373 1101 50-51 -0.8141920940151337 1101 52-53 -0.59845398291219 1101 54-55 -0.5756649581630384 1101 56-57 -0.6073297152420665 1101 58-59 -0.6947328099711427 1101 60-61 -0.6444933427330568 1101 62-63 -0.6666215690244996 1101 64-65 -0.6622972237920663 1101 66-67 -0.6784727055667901 1101 68-69 -0.7371466063128835 1101 70-71 -0.6731809994983209 1101 72-73 -0.7912646367597844 1101 74-75 -0.787772468319659 1101 76-77 -0.8261755256570353 1101 78-79 -0.8439626554766164 1101 80-81 -0.9242600948120554 1101 82-83 -0.9794156070007709 1101 84-85 -0.920138033418997 1101 86-87 -0.926557625125966 1101 88-89 -1.0648319840895404 1101 90-91 -0.9887023917999294 1101 92-93 -1.0496484280200917 1101 94-95 -1.1428613470605704 1101 96-97 -1.2374066581635894 1101 98-99 -1.3038630011824637 1101 100 -1.3015081870602216 1102 1 -0.4397928198458345 1102 2 -0.4433614751967454 1102 3 -0.4142487582458685 1102 4 -0.3033280547413213 1102 5 -0.3264217104900098 1102 6 -0.2935221605086511 1102 7 -0.35491245527414605 1102 8 -0.38209744399638623 1102 9 -0.46646715898602764 1102 10-11 -0.40523409839835267 1102 12-13 -0.49624312337740406 1102 14-15 -0.6224445561338428 1102 16-17 -0.6584775964542189 1102 18-19 -0.6224992114891776 1102 20-21 -0.6801408452936997 1102 22-23 -0.7448529418664833 1102 24-25 -0.7152919896972918 1102 26-27 -0.6838611498823184 1102 28-29 -0.8143835804439945 1102 30-31 -0.806512315851343 1102 32-33 -0.7422894019283248 1102 34-35 -0.748615213108593 1102 36-37 -0.8014410974183406 1102 38-39 -0.9824194886075404 1102 40-41 -1.0111807847120104 1102 42-43 -0.8824631278459094 1102 44-45 -0.9092338664179778 1102 46-47 -0.9566344253953076 1102 48-49 -0.9448175630516928 1102 50-51 -0.8205088776672795 1102 52-53 -0.6917893935953998 1102 54-55 -0.6638567878213664 1102 56-57 -0.7270243630318802 1102 58-59 -0.7511315190562655 1102 60-61 -0.7409995671759262 1102 62-63 -0.6627071237597733 1102 64-65 -0.7250341013250008 1102 66-67 -0.7117170364081957 1102 68-69 -0.7472167897608486 1102 70-71 -0.7318688511078975 1102 72-73 -0.7791649159561231 1102 74-75 -0.8161250999876017 1102 76-77 -0.81112311918789 1102 78-79 -0.8814792415389725 1102 80-81 -0.8915731618216753 1102 82-83 -1.0103609160400424 1102 84-85 -0.9503236286545338 1102 86-87 -0.8961708157552764 1102 88-89 -0.979065408505047 1102 90-91 -1.0565492078417016 1102 92-93 -1.0585838559170853 1102 94-95 -1.0890089966315628 1102 96-97 -1.1747165760938607 1102 98-99 -1.108066534770714 1102 100 -1.1175040184606075 1103 1 -0.39941253534451704 1103 2 -0.4995535077161506 1103 3 -0.45247680197208595 1103 4 -0.3923241207037407 1103 5 -0.3466914836273176 1103 6 -0.43916249720000167 1103 7 -0.4336121225746723 1103 8 -0.43293093206185773 1103 9 -0.5589614179524105 1103 10-11 -0.5588857045180049 1103 12-13 -0.7100021230264844 1103 14-15 -0.7375068823863131 1103 16-17 -0.7502766587556877 1103 18-19 -0.7139746625641763 1103 20-21 -0.8506670135793684 1103 22-23 -0.8298571359410332 1103 24-25 -0.7908304466866127 1103 26-27 -0.825777022269385 1103 28-29 -0.8234312689631835 1103 30-31 -0.8193466949130581 1103 32-33 -0.8870904236388029 1103 34-35 -0.9871258404890924 1103 36-37 -1.0122374910795244 1103 38-39 -1.033299026698387 1103 40-41 -1.0090994996771343 1103 42-43 -1.02741427959662 1103 44-45 -1.032814534191182 1103 46-47 -1.0108007677404984 1103 48-49 -1.0924472151805062 1103 50-51 -1.0182505565929318 1103 52-53 -0.8679544831537882 1103 54-55 -0.7739331334583355 1103 56-57 -0.7805352534932624 1103 58-59 -0.8321577671358753 1103 60-61 -0.8414661661228209 1103 62-63 -0.8497746655363585 1103 64-65 -0.7800245282248284 1103 66-67 -0.8304125096341721 1103 68-69 -0.8531951071129313 1103 70-71 -0.8183353726524842 1103 72-73 -0.9562321229024988 1103 74-75 -0.8993835132489636 1103 76-77 -0.8426068344813231 1103 78-79 -0.8484277272584642 1103 80-81 -0.9143303755646741 1103 82-83 -0.9962200577528009 1103 84-85 -0.9696928389726942 1103 86-87 -1.0811329495021198 1103 88-89 -1.078719848505859 1103 90-91 -1.1071660237977063 1103 92-93 -1.1115622032734294 1103 94-95 -1.289002495707301 1103 96-97 -1.3157337554928432 1103 98-99 -1.4123442516461182 1103 100 -1.488860511318503 1104 1 -0.4046738254649185 1104 2 -0.4289781417644143 1104 3 -0.461210024717122 1104 4 -0.37563714924852576 1104 5 -0.27023000971568223 1104 6 -0.24691840856436187 1104 7 -0.30719712320089343 1104 8 -0.3446771201122587 1104 9 -0.4248663455729087 1104 10-11 -0.4906898561866342 1104 12-13 -0.45843349165294 1104 14-15 -0.6081793846446004 1104 16-17 -0.5706083725653244 1104 18-19 -0.6096114190660131 1104 20-21 -0.6088753267701819 1104 22-23 -0.5556687963201128 1104 24-25 -0.6925482647850032 1104 26-27 -0.7302830944989651 1104 28-29 -0.7189475907038272 1104 30-31 -0.7615409358159937 1104 32-33 -0.8078577477876081 1104 34-35 -0.8008701410401144 1104 36-37 -0.7781485479121173 1104 38-39 -0.7758534279781131 1104 40-41 -0.7883449606816697 1104 42-43 -0.7411236776898988 1104 44-45 -0.8088625416705213 1104 46-47 -0.8944710889144076 1104 48-49 -0.7487468693801489 1104 50-51 -0.6992947551287614 1104 52-53 -0.554806620913368 1104 54-55 -0.5775536249892284 1104 56-57 -0.5499225419117479 1104 58-59 -0.5863344633820233 1104 60-61 -0.527549939044448 1104 62-63 -0.5599973666269236 1104 64-65 -0.6041434885699957 1104 66-67 -0.601920383064666 1104 68-69 -0.5348053586032222 1104 70-71 -0.6124380012012693 1104 72-73 -0.6707222347063535 1104 74-75 -0.7111134578232239 1104 76-77 -0.6290186373007387 1104 78-79 -0.6601701909612352 1104 80-81 -0.7512247314648235 1104 82-83 -0.8576290008052894 1104 84-85 -0.830613802037913 1104 86-87 -0.8777307207035285 1104 88-89 -0.7475908412541337 1104 90-91 -0.7793965377183092 1104 92-93 -0.7930974942870357 1104 94-95 -0.8881477738889352 1104 96-97 -0.874957006616345 1104 98-99 -0.9298154323781702 1104 100 -1.0382346134181937 1105 1 -0.45020751300353723 1105 2 -0.491108827120172 1105 3 -0.4225887401473578 1105 4 -0.3709065645454288 1105 5 -0.30875282174603313 1105 6 -0.33500492775869617 1105 7 -0.316328830306297 1105 8 -0.3562709239716426 1105 9 -0.4791491178524936 1105 10-11 -0.5160714102614534 1105 12-13 -0.561104369914375 1105 14-15 -0.5559735425833736 1105 16-17 -0.6158209437337945 1105 18-19 -0.6112173051681609 1105 20-21 -0.6832224507388744 1105 22-23 -0.6994146785003537 1105 24-25 -0.6860165852321884 1105 26-27 -0.6648715508881864 1105 28-29 -0.6873173580898921 1105 30-31 -0.7537827769029022 1105 32-33 -0.6751867529664182 1105 34-35 -0.7109983690517439 1105 36-37 -0.7566562919443172 1105 38-39 -0.7978530179101782 1105 40-41 -0.8484338096443906 1105 42-43 -0.8652286736730943 1105 44-45 -0.9075688143576386 1105 46-47 -0.881574207781874 1105 48-49 -0.8404444697587934 1105 50-51 -0.7984320943472341 1105 52-53 -0.660680332682773 1105 54-55 -0.623776693743757 1105 56-57 -0.6918824841094349 1105 58-59 -0.5647013967187533 1105 60-61 -0.5975092699695992 1105 62-63 -0.7457011850438988 1105 64-65 -0.6694762520509485 1105 66-67 -0.6771101994807296 1105 68-69 -0.6367564462458475 1105 70-71 -0.5884349000611664 1105 72-73 -0.6550721459326141 1105 74-75 -0.7175896167412006 1105 76-77 -0.7632372330980672 1105 78-79 -0.6770495812892712 1105 80-81 -0.7320610426062792 1105 82-83 -0.8076348723327449 1105 84-85 -0.7831876393152228 1105 86-87 -0.8438148099363509 1105 88-89 -0.73403685578268 1105 90-91 -0.8358843615300593 1105 92-93 -0.8826345372779585 1105 94-95 -0.8840459771616658 1105 96-97 -0.9425591550634813 1105 98-99 -1.068302748761532 1105 100 -1.0358642746899704 1106 1 -0.5324232976885774 1106 2 -0.5497606425795993 1106 3 -0.5063070293821639 1106 4 -0.4009724907525154 1106 5 -0.373354519680575 1106 6 -0.4246633571640004 1106 7 -0.4554615703491436 1106 8 -0.43968815378001125 1106 9 -0.5322728259856149 1106 10-11 -0.5831205210920984 1106 12-13 -0.5511835304234722 1106 14-15 -0.6437628616600648 1106 16-17 -0.7613463175026993 1106 18-19 -0.7770906880209552 1106 20-21 -0.7444205936334853 1106 22-23 -0.7213279141701037 1106 24-25 -0.7880570077994165 1106 26-27 -0.7455510820301043 1106 28-29 -0.726628826581738 1106 30-31 -0.8572676151319385 1106 32-33 -0.7916848983902156 1106 34-35 -0.8143818016312352 1106 36-37 -0.8346273037327947 1106 38-39 -0.8410225183657332 1106 40-41 -0.8725768430508793 1106 42-43 -0.8219184492767226 1106 44-45 -0.816283420081021 1106 46-47 -0.8250551954205392 1106 48-49 -0.7965550225120879 1106 50-51 -0.7617990456129604 1106 52-53 -0.6511183717682059 1106 54-55 -0.7823521225535188 1106 56-57 -0.7431340989281594 1106 58-59 -0.778736386062981 1106 60-61 -0.7847913037540692 1106 62-63 -0.695334802988981 1106 64-65 -0.713071843464526 1106 66-67 -0.7593786624252701 1106 68-69 -0.6442798537193895 1106 70-71 -0.6658821122404035 1106 72-73 -0.7271606780310194 1106 74-75 -0.7638310372060246 1106 76-77 -0.7192930607558736 1106 78-79 -0.6721362229045695 1106 80-81 -0.818631558121119 1106 82-83 -0.8126520005418847 1106 84-85 -0.79482072851021 1106 86-87 -0.7564866795014247 1106 88-89 -0.8577025289564126 1106 90-91 -0.7608087499806864 1106 92-93 -0.8058095156378258 1106 94-95 -0.9241101894212882 1106 96-97 -0.9229047695079338 1106 98-99 -0.8676533210235533 1106 100 -1.0169685830068111 1107 1 -0.513780857052577 1107 2 -0.5040062560845833 1107 3 -0.4664948813027934 1107 4 -0.3799382387585908 1107 5 -0.31745328579453513 1107 6 -0.35962355523235345 1107 7 -0.3115715889039379 1107 8 -0.35939968630280106 1107 9 -0.4832439902695782 1107 10-11 -0.5553244973503908 1107 12-13 -0.5643869538695583 1107 14-15 -0.7063748625278521 1107 16-17 -0.7403372866819069 1107 18-19 -0.6961882593798734 1107 20-21 -0.6473217395653208 1107 22-23 -0.7169160560867525 1107 24-25 -0.7736113074217315 1107 26-27 -0.7233770631386349 1107 28-29 -0.7111955325548465 1107 30-31 -0.6821168925654533 1107 32-33 -0.7108910012866758 1107 34-35 -0.7008419727292363 1107 36-37 -0.7421749967493056 1107 38-39 -0.8174434819943173 1107 40-41 -0.8309225483293545 1107 42-43 -0.8469123377344729 1107 44-45 -0.8639962605961387 1107 46-47 -0.8223113041223087 1107 48-49 -0.7493642365455173 1107 50-51 -0.7538506403728178 1107 52-53 -0.7392863196838775 1107 54-55 -0.6187854672601176 1107 56-57 -0.6598123364787725 1107 58-59 -0.5680048109529423 1107 60-61 -0.5409387299318098 1107 62-63 -0.6057144962955903 1107 64-65 -0.6044147624741569 1107 66-67 -0.5791451046616842 1107 68-69 -0.5721387797273323 1107 70-71 -0.6097016137158491 1107 72-73 -0.5355405087744458 1107 74-75 -0.5540405245383155 1107 76-77 -0.5695135999979684 1107 78-79 -0.5699609885672103 1107 80-81 -0.5350838195331349 1107 82-83 -0.6671390200946625 1107 84-85 -0.6197731557947748 1107 86-87 -0.6806865591972553 1107 88-89 -0.6235838212946909 1107 90-91 -0.72107403007724 1107 92-93 -0.6800578764344252 1107 94-95 -0.6844826161611834 1107 96-97 -0.7480032298616095 1107 98-99 -0.7324090008673423 1107 100 -0.7404102671809873 1108 1 -0.49854022707918233 1108 2 -0.4409953976647145 1108 3 -0.5159860994526362 1108 4 -0.35952618391043245 1108 5 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26-27 0.9252240170625257 2311 28-29 0.8565273954135435 2311 30-31 0.9079665780247197 2311 32-33 0.922788179212489 2311 34-35 0.9295246445964906 2311 36-37 0.8822843550377044 2311 38-39 0.9363451149155608 2311 40-41 0.9727938203571327 2311 42-43 0.9940678078979275 2311 44-45 1.012610247723785 2311 46-47 1.0169797623107328 2311 48-49 0.9939887125932145 2311 50-51 1.003678096080705 2311 52-53 0.8260172934408629 2311 54-55 0.8145047300297534 2311 56-57 0.7968483103743651 2311 58-59 0.8006004462710479 2311 60-61 0.8247168518922976 2311 62-63 0.8446856006337526 2311 64-65 0.809277071638725 2311 66-67 0.8149540337773047 2311 68-69 0.8516563518694724 2311 70-71 0.8083034781339222 2311 72-73 0.7294844608519853 2311 74-75 0.7809606545515422 2311 76-77 0.8285539249626979 2311 78-79 0.919356038811177 2311 80-81 0.8675857136382916 2311 82-83 0.8463535301723333 2311 84-85 0.9206447692344604 2311 86-87 0.9261017628795436 2311 88-89 0.9695337915457891 2311 90-91 1.0165975415476645 2311 92-93 0.9664768553436218 2311 94-95 1.055955221182547 2311 96-97 1.0540619661548938 2311 98-99 1.1019561889866623 2311 100 1.2181144038208913 2312 1 0.5609604848523126 2312 2 0.5413586722836534 2312 3 0.5773518064901069 2312 4 0.4772820229358885 2312 5 0.48507002825675016 2312 6 0.5125626573118254 2312 7 0.5026537610173136 2312 8 0.5301786596459692 2312 9 0.6865475502460896 2312 10-11 0.679344952967817 2312 12-13 0.6822611776655094 2312 14-15 0.8277095567199879 2312 16-17 0.8200330248959631 2312 18-19 0.7849612960814127 2312 20-21 0.8725527128860264 2312 22-23 0.8691586520657992 2312 24-25 0.9086647026293093 2312 26-27 0.9421692746286041 2312 28-29 0.9134418429673801 2312 30-31 0.9387135321384505 2312 32-33 0.8717716858450686 2312 34-35 0.857724237869725 2312 36-37 0.8985267104938046 2312 38-39 0.9820323509323075 2312 40-41 0.9628413639224931 2312 42-43 0.9515490643712994 2312 44-45 0.9652121400195952 2312 46-47 0.9218359435419075 2312 48-49 0.8763140152000304 2312 50-51 0.9428524733514791 2312 52-53 0.8307562989218553 2312 54-55 0.7924596070416143 2312 56-57 0.6943734941941315 2312 58-59 0.748767190813652 2312 60-61 0.7887961118430198 2312 62-63 0.7527950608590146 2312 64-65 0.7183348558566323 2312 66-67 0.6954058901232614 2312 68-69 0.8075532248735797 2312 70-71 0.8307205401122317 2312 72-73 0.771371828580854 2312 74-75 0.793911730741776 2312 76-77 0.8258051074309094 2312 78-79 0.8072086457995518 2312 80-81 0.8845282483872126 2312 82-83 0.9179207857494411 2312 84-85 0.8888400006557191 2312 86-87 0.9163163010822437 2312 88-89 0.934872675824586 2312 90-91 1.0062489152168617 2312 92-93 1.008641470137654 2312 94-95 1.0724932911931226 2312 96-97 1.1340301526725796 2312 98-99 1.1545155518896237 2312 100 1.2270310811091925 2313 1 0.44388362811433524 2313 2 0.45633641600160146 2313 3 0.499397425810848 2313 4 0.4191714848340382 2313 5 0.4130530287498857 2313 6 0.4498868035467538 2313 7 0.445490717983823 2313 8 0.428818526105502 2313 9 0.5793255875725265 2313 10-11 0.6292920942632847 2313 12-13 0.6139266799210006 2313 14-15 0.7946639153317605 2313 16-17 0.7554626904038457 2313 18-19 0.7431522488324802 2313 20-21 0.7649393052621392 2313 22-23 0.7016457323274707 2313 24-25 0.6688714289772548 2313 26-27 0.7188307409733028 2313 28-29 0.7709538711348074 2313 30-31 0.8191290421563693 2313 32-33 0.8314271047830317 2313 34-35 0.8183798255297177 2313 36-37 0.8059082757241427 2313 38-39 0.7888967863300635 2313 40-41 0.9151253510707846 2313 42-43 0.7626148682083951 2313 44-45 0.850926002760481 2313 46-47 0.8945147778040905 2313 48-49 0.8916811932594939 2313 50-51 0.8212820445457965 2313 52-53 0.7507096202230485 2313 54-55 0.6938285128246449 2313 56-57 0.727109535786802 2313 58-59 0.7106628722227697 2313 60-61 0.7052869062626286 2313 62-63 0.6862639842627516 2313 64-65 0.7307692133949715 2313 66-67 0.6865044273093588 2313 68-69 0.6648302557728343 2313 70-71 0.6083164613306309 2313 72-73 0.6381070239019735 2313 74-75 0.6915249599683122 2313 76-77 0.639177695019157 2313 78-79 0.7249260502060118 2313 80-81 0.6982319280078713 2313 82-83 0.7275950244294016 2313 84-85 0.6905000919408231 2313 86-87 0.7860972811193783 2313 88-89 0.8308263384233321 2313 90-91 0.8192139057561185 2313 92-93 0.8482317104193342 2313 94-95 0.9234295785555133 2313 96-97 0.9817002611281858 2313 98-99 0.9953076108872203 2313 100 1.0964555263530436 2314 1 0.32190161819804786 2314 2 0.24113273772119115 2314 3 0.3263993686834823 2314 4 0.3001410898659884 2314 5 0.24679166486329507 2314 6 0.27809500119599306 2314 7 0.31714674798870135 2314 8 0.3150345400014558 2314 9 0.4250756116961085 2314 10-11 0.4499562832645836 2314 12-13 0.49493094000695237 2314 14-15 0.581952553758029 2314 16-17 0.6384548547260636 2314 18-19 0.5700895947670759 2314 20-21 0.5873538843410913 2314 22-23 0.6021645060230298 2314 24-25 0.608270770202104 2314 26-27 0.6934055541703046 2314 28-29 0.6307324098472051 2314 30-31 0.6867754899871699 2314 32-33 0.6994302559210297 2314 34-35 0.6905884359824128 2314 36-37 0.7030331952198239 2314 38-39 0.7430110022546117 2314 40-41 0.7549542979106505 2314 42-43 0.7037163971056515 2314 44-45 0.7757115046392684 2314 46-47 0.6132779391332761 2314 48-49 0.542987861623125 2314 50-51 0.6729939911309941 2314 52-53 0.6460737042373665 2314 54-55 0.5892334730019044 2314 56-57 0.6170673625209204 2314 58-59 0.6511147307479135 2314 60-61 0.5133048787192891 2314 62-63 0.5958335901127043 2314 64-65 0.616027727359544 2314 66-67 0.587355305627348 2314 68-69 0.7007229833908113 2314 70-71 0.6650535842307264 2314 72-73 0.7004607650111083 2314 74-75 0.7569869557680917 2314 76-77 0.7075392784299908 2314 78-79 0.7286142141561918 2314 80-81 0.7136917624284536 2314 82-83 0.7161391535609773 2314 84-85 0.7531945893861511 2314 86-87 0.7718364854021509 2314 88-89 0.6065699241295057 2314 90-91 0.6394696051078483 2314 92-93 0.8725392562381984 2314 94-95 0.9476311382987284 2314 96-97 0.9567784160900779 2314 98-99 0.8982966502641361 2314 100 0.9555060541654221 2315 1 0.2479046845573265 2315 2 0.2959727039683031 2315 3 0.29647117094133435 2315 4 0.212674963747439 2315 5 0.2426117461419537 2315 6 0.23283532256396455 2315 7 0.2305630328465753 2315 8 0.23929201705753655 2315 9 0.13485210251727864 2315 10-11 0.2778810877747091 2315 12-13 0.26714792210187 2315 14-15 0.42757300095637163 2315 16-17 0.2474870022234157 2315 18-19 0.39868566950674733 2315 20-21 0.31909041893477763 2315 22-23 0.33627485989390493 2315 24-25 0.45786007082394775 2315 26-27 0.5014039824156242 2315 28-29 0.37014028869281645 2315 30-31 0.41755882181518444 2315 32-33 0.4617664299615285 2315 34-35 0.3776880895862078 2315 36-37 0.5733845321057203 2315 38-39 0.5755068533797427 2315 40-41 0.6107090139703502 2315 42-43 0.5924711162381797 2315 44-45 0.592619540144554 2315 46-47 0.5995356759535824 2315 48-49 0.5206869390266178 2315 50-51 0.5041313751877468 2315 52-53 0.21148477564794632 2315 54-55 0.34662379010631383 2315 56-57 0.3668649807232569 2315 58-59 0.4057355943152814 2315 60-61 0.43023179432137937 2315 62-63 0.4399399462811857 2315 64-65 0.4795735321781649 2315 66-67 0.3761007747059111 2315 68-69 0.5136675086373828 2315 70-71 0.3964080379581745 2315 72-73 0.45628705067120734 2315 74-75 0.20327248362996642 2315 76-77 0.439108391573825 2315 78-79 0.4931182384166526 2315 80-81 0.4922021421330953 2315 82-83 0.4410641818941521 2315 84-85 0.5085142209335771 2315 86-87 0.5006574079240416 2315 88-89 0.5047053955664715 2315 90-91 0.6292382470325641 2315 92-93 0.7022620116946996 2315 94-95 0.7131456448745368 2315 96-97 0.7335424314239631 2315 98-99 0.915453655299963 2315 100 0.9394426214299259 2316 1 0.045723069494613355 2316 2 0.05440003159833395 2316 3 0.09812098462103691 2316 4 0.029507240765262566 2316 5 -0.02301199930811748 2316 6 -0.14432971779780246 2316 7 -0.11587771384876078 2316 8 -0.028996192766143736 2316 9 -0.01153759431211654 2316 10-11 0.020518031116601776 2316 12-13 -0.017553115124059104 2316 14-15 -0.14911056111458265 2316 16-17 -0.009139137799742514 2316 18-19 0.07627062536469253 2316 20-21 0.1459408993721567 2316 22-23 0.016763729447120568 2316 24-25 -0.0019058010095847067 2316 26-27 -0.08452359599128556 2316 28-29 -0.07168864729550961 2316 30-31 -0.591542994448325 2316 32-33 -0.40815095902418363 2316 34-35 -0.5115095154573623 2316 36-37 -0.29574822075989715 2316 38-39 -0.16357744417558706 2316 40-41 -0.0855142010892962 2316 42-43 -0.030307777996590346 2316 44-45 -0.14732048691166 2316 46-47 -0.3057718808534986 2316 48-49 -0.2090890373579768 2316 50-51 0.004029347334579825 2316 52-53 -0.3080132728713636 2316 54-55 -0.24389665230881263 2316 56-57 -0.23822426709306654 2316 58-59 -0.6519176500559851 2316 60-61 -0.17180407198974024 2316 62-63 -0.11636566522145131 2316 64-65 -0.41693127709404365 2316 66-67 -0.7255818076846694 2316 68-69 -0.6392765959123992 2316 70-71 -0.4583238394991369 2316 72-73 -0.6628431731282518 2316 74-75 -0.504825122989601 2316 76-77 -0.19063741438258575 2316 78-79 -0.23595680062680202 2316 80-81 -0.31087005828651115 2316 82-83 -0.034441466282608246 2316 84-85 -0.02020427036292105 2316 86-87 0.1067897091783081 2316 88-89 0.10435149402136545 2316 90-91 -0.44990150415843644 2316 92-93 -0.5304147237078993 2316 94-95 -0.1503162761210568 2316 96-97 0.12528756480850944 2316 98-99 0.24861259842930394 2316 100 0.14225434667047665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 288.0 1 800.0 2 450.0 3 454.0 4 423.0 5 349.0 6 443.0 7 1010.0 8 2557.0 9 4609.0 10 7629.0 11 12523.0 12 19370.0 13 25183.0 14 30018.0 15 34896.0 16 41212.0 17 50111.0 18 61208.0 19 74462.0 20 92348.0 21 115848.0 22 126151.0 23 122223.0 24 116422.0 25 118816.0 26 130515.0 27 149189.0 28 172878.0 29 205796.0 30 247345.0 31 306056.0 32 383450.0 33 501520.0 34 696023.0 35 949414.0 36 1387307.0 37 1923386.0 38 1845985.0 39 480557.0 40 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13445820997058 21.608401122960323 14.010798432454703 22.24634223461439 2 14.779738872346648 20.78504197893142 42.11396404819786 22.321255100524077 3 18.036306632647342 28.42545080060094 29.666119510074974 23.87212305667674 4 11.75150691666943 17.62439571477488 40.937086887731574 29.687010480824117 5 12.190466737189464 39.42099129783439 35.142092001494255 13.24644996348189 6 28.134768554323443 39.624893264063985 18.69482674517448 13.54551143643809 7 26.12038973177693 33.37737926875889 23.681542520092606 16.820688479371583 8 25.56733956351544 35.514213303672555 20.665606230527086 18.25284090228492 9 26.60622932383998 15.82391491243906 22.013079425967895 35.556776337753064 10-11 24.402949393700975 27.25917615317268 28.06165758961766 20.27621686350868 12-13 25.112285931809613 25.1614492074512 28.548080686992215 21.178184173746974 14-15 22.82160423756346 26.222503894297976 26.50785976723182 24.44803210090674 16-17 20.782995585835863 29.24024026139142 27.71784786655441 22.2589162862183 18-19 20.393312999597242 28.56967220930109 30.50359392349081 20.533420867610857 20-21 21.918077416324532 27.559454803979406 30.01548583334 20.506981946356063 22-23 22.156571325943865 27.425719023066108 29.558682860664447 20.859026790325576 24-25 21.54015477359161 27.655617711423798 30.20675986963579 20.597467645348804 26-27 21.226743980699865 27.949376675735234 29.67544716344844 21.14843218011647 28-29 20.69523057044427 27.855915970725924 30.275976468644377 21.17287699018543 30-31 21.430210948821653 27.631938971675645 30.062193335267352 20.875656744235346 32-33 21.5027547324953 28.119704602976398 29.428080350597092 20.949460313931212 34-35 21.53349636035482 27.946779539096156 29.93395766862223 20.585766431926793 36-37 21.41517165455503 27.628449593029096 29.613122732182706 21.343256020233166 38-39 21.47822053130711 27.744635592411832 30.079011755760522 20.69813212052054 40-41 21.24754531434305 27.107401968080673 30.55784489693267 21.0872078206436 42-43 21.15367224853383 27.167768425171996 29.938328328651174 21.740230997643 44-45 21.212726510643655 27.921233333962743 29.6097408646446 21.25629929074901 46-47 21.296158688990268 27.702390185389337 29.126994027711568 21.874457097908827 48-49 21.76954502746935 27.61043871161606 29.186578059938217 21.433438200976372 50-51 21.72911385938598 27.537017391593132 28.771618128416282 21.962250620604603 52-53 21.17067718338977 28.128206878134748 28.749855981037996 21.951259957437486 54-55 21.328886600649337 28.07653956841979 28.58953416647914 22.005039664451733 56-57 21.834393326990337 27.7294561114512 28.257991309384252 22.17815925217421 58-59 21.152655778285347 27.83006089750148 29.06759623143667 21.9496870927765 60-61 21.375391371655134 28.464767937614955 28.337040100119843 21.82280059061007 62-63 21.574466888075907 27.82076461377908 28.463978539376622 22.140789958768387 64-65 20.970262200997844 27.714022161706342 28.67559338471662 22.6401222525792 66-67 21.45083556558305 27.511456437933727 28.42484706579622 22.612860930687 68-69 20.99612771737177 27.75302176734464 28.614640834069032 22.636209681214556 70-71 21.22272357936894 27.26192144946768 28.799035975749877 22.716318995413502 72-73 21.53459913533073 27.57616560028724 28.57892393091924 22.310311333462792 74-75 21.5442968678719 27.844900391563932 28.422925849736668 22.1878768908275 76-77 21.17482404350175 27.685159370291522 28.515178928521077 22.62483765768565 78-79 21.11806540822197 27.524389722567445 28.526744969125488 22.830799900085097 80-81 20.959389808589595 28.030035302666377 28.546165825243797 22.46440906350023 82-83 21.706353787330208 27.303944492995996 29.13183737684945 21.857864342824342 84-85 21.595663333034928 27.456503073917602 28.400507568770887 22.54732602427658 86-87 20.785329432527437 27.924764585622917 28.921594961188024 22.368311020661626 88-89 21.47798603182661 27.03759720502339 28.82970946111162 22.654707302038386 90-91 21.39915989364206 27.108736119746958 28.938932566177872 22.55317142043311 92-93 21.59371605135617 27.305147815675507 28.700903955100138 22.400232177868187 94-95 20.735213421473333 26.969230250082347 29.218670491571313 23.076885836873007 96-97 21.161790106308267 26.912298794314044 28.936573176972047 22.989337922405642 98-99 20.752897811869403 27.24039745775138 28.852242021247655 23.154462709131558 100 21.27118669265126 26.82069816756455 28.222611562682744 23.68550357710145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2384.0 1 1699.5 2 1267.0 3 1559.0 4 1365.0 5 1310.5 6 2073.0 7 3737.5 8 6070.0 9 7593.5 10 7117.5 11 6539.5 12 7154.5 13 8140.0 14 9337.5 15 10659.0 16 11868.5 17 13141.5 18 14295.0 19 15317.0 20 16788.0 21 18602.5 22 20632.5 23 23688.5 24 27464.5 25 31698.0 26 38799.0 27 47365.0 28 56211.5 29 73691.0 30 101743.5 31 130777.0 32 172083.0 33 225722.5 34 278756.5 35 326486.0 36 383815.5 37 450619.5 38 468464.5 39 448339.0 40 414494.0 41 376244.0 42 354649.5 43 354323.0 44 368566.0 45 397214.5 46 414971.0 47 414821.5 48 414248.5 49 400774.0 50 379897.5 51 365062.5 52 347470.5 53 323918.0 54 301503.5 55 262536.5 56 227763.5 57 186099.0 58 143754.5 59 127584.5 60 104631.5 61 76138.0 62 57871.5 63 47167.5 64 34753.5 65 19959.0 66 12652.0 67 9062.0 68 6857.5 69 5328.0 70 4061.0 71 3033.5 72 2276.5 73 1779.5 74 1422.0 75 1012.5 76 681.5 77 468.5 78 314.0 79 208.5 80 147.0 81 117.5 82 89.0 83 63.5 84 50.0 85 39.5 86 38.5 87 35.5 88 23.0 89 20.0 90 17.5 91 9.5 92 6.0 93 5.0 94 5.5 95 5.0 96 2.5 97 1.5 98 2.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026246618592568185 2 0.11102128083498732 3 0.017654203673760277 4 0.01821936808870974 5 0.02062371161671507 6 0.04607526840520181 7 0.046736223398956264 8 0.03937950694673277 9 0.033603335044791675 10-11 0.03708532258435319 12-13 0.036448315235299976 14-15 0.03207547531285203 16-17 0.03884786923436506 18-19 0.03744453725487191 20-21 0.04851313863578889 22-23 0.04826887265983616 24-25 0.06681871824542313 26-27 0.05887767926248918 28-29 0.05136290835523745 30-31 0.049839838152238046 32-33 0.044734200301931905 34-35 0.04568252703210134 36-37 0.04115163265462515 38-39 0.046491957423003535 40-41 0.055778854038147634 42-43 0.05789103630079775 44-45 0.06054922486263631 46-47 0.06230698198370794 48-49 0.06176097568451948 50-51 0.05617638494018836 52-53 0.0650034867770685 54-55 0.05184186124926241 56-57 0.05639191374249959 58-59 0.053609197428214544 60-61 0.04927946326622884 62-63 0.05574532733556589 64-65 0.05394446445403201 66-67 0.052296866498586135 68-69 0.05803951169794548 70-71 0.05836041013694221 72-73 0.06052048768899482 74-75 0.05157364762860843 76-77 0.05238786754845088 78-79 0.06285777781183664 80-81 0.062426720207214174 82-83 0.05281413562413309 84-85 0.05914110335420291 86-87 0.056224280229590855 88-89 0.06440000613059704 90-91 0.0700085445196294 92-93 0.07454901795498609 94-95 0.07812679607335259 96-97 0.07377790379560589 98-99 0.08233679201183206 100 0.12702788655330172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.043944E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.692961165836095 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4176539431112 34.288440888411984 2 15.57739054328846 15.170185455788149 3 6.104691626059812 8.917665368314056 4 2.9217469365091473 5.69074040463362 5 1.6198597945569007 3.94378850352292 6 0.9678696203955912 2.8277062703709026 7 0.6250012453753757 2.1303212958763655 8 0.394161885024089 1.5354327488424573 9 0.2837613843312014 1.2435463860842773 >10 0.9785273839631361 7.601035234106118 >50 0.046312629806658435 1.5750854638747636 >100 0.04740128473013491 5.06060243878539 >500 0.009944116902948014 3.399631181599115 >1k 0.005447876225110741 4.575116181926769 >5k 1.3127412585725064E-4 0.40791351510275337 >10k+ 9.845559439293798E-5 1.6327886627604724 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46581 0.4462020951315396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35025 0.3355065022644893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20569 0.1970316415439909 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.831623152199735E-5 0.0 10-11 0.0 0.0 0.0 5.268481834274635E-5 0.0 12-13 0.0 0.0 0.0 5.747434728299602E-5 0.0 14-15 0.0 0.0 0.0 8.621152092449403E-5 0.0 16-17 0.0 0.0 0.0 9.579057880499337E-5 0.0 18-19 0.0 0.0 0.0 1.1015916562574237E-4 0.0 20-21 0.0 0.0 0.0 2.0116021549048608E-4 0.0 22-23 0.0 0.0 0.0 4.981110097859655E-4 0.0 24-25 0.0 0.0 0.0 0.0012117508218831663 0.0 26-27 0.0 0.0 0.0 0.0027779267853448074 9.579057880499338E-6 28-29 0.0 0.0 0.0 0.0067676043925727816 9.579057880499338E-6 30-31 0.0 0.0 0.0 0.020408182814403836 9.579057880499338E-6 32-33 0.0 0.0 0.0 0.052871609971416086 9.579057880499338E-6 34-35 0.0 0.0 0.0 0.10178706903818596 9.579057880499338E-6 36-37 0.0 0.0 0.0 0.1798420221774348 9.579057880499338E-6 38-39 0.0 0.0 0.0 0.29730042990811767 9.579057880499338E-6 40-41 0.0 0.0 0.0 0.42357157088885994 9.579057880499338E-6 42-43 0.0 0.0 0.0 0.549780447993379 9.579057880499338E-6 44-45 0.0 0.0 0.0 0.7022694703930479 9.579057880499338E-6 46-47 0.0 9.579057880499338E-6 0.0 0.8808278988144957 9.579057880499338E-6 48-49 0.0 9.579057880499338E-6 0.0 1.0748804533576513 9.579057880499338E-6 50-51 0.0 9.579057880499338E-6 0.0 1.279388549577372 9.579057880499338E-6 52-53 0.0 9.579057880499338E-6 0.0 1.4787862184178462 9.579057880499338E-6 54-55 0.0 9.579057880499338E-6 0.0 1.6947460783337038 9.579057880499338E-6 56-57 0.0 9.579057880499338E-6 0.0 1.9447211727832143 9.579057880499338E-6 58-59 0.0 9.579057880499338E-6 0.0 2.2311302138812046 9.579057880499338E-6 60-61 0.0 9.579057880499338E-6 0.0 2.528751542228319 9.579057880499338E-6 62-63 0.0 9.579057880499338E-6 0.0 2.8309995555317142 9.579057880499338E-6 64-65 0.0 9.579057880499338E-6 0.0 3.1365762914485833 9.579057880499338E-6 66-67 0.0 9.579057880499338E-6 0.0 3.469558711961561 9.579057880499338E-6 68-69 0.0 1.4368586820749007E-5 0.0 3.834903979523806 9.579057880499338E-6 70-71 0.0 1.9158115760998676E-5 0.0 4.238009893250979 9.579057880499338E-6 72-73 0.0 1.9158115760998676E-5 0.0 4.641752814327205 9.579057880499338E-6 74-75 0.0 1.9158115760998676E-5 0.0 5.029508287800878 9.579057880499338E-6 76-77 0.0 1.9158115760998676E-5 0.0 5.4534199152444955 9.579057880499338E-6 78-79 0.0 1.9158115760998676E-5 0.0 5.9127980044906625 9.579057880499338E-6 80-81 0.0 2.3947644701248343E-5 0.0 6.409141678097676 9.579057880499338E-6 82-83 0.0 2.873717364149801E-5 0.0 6.913622761374173 9.579057880499338E-6 84-85 0.0 2.873717364149801E-5 0.0 7.395118895266412 9.579057880499338E-6 86-87 0.0 3.831623152199735E-5 0.0 7.890471136382795 9.579057880499338E-6 88 0.0 3.831623152199735E-5 0.0 8.274342301885925 9.579057880499338E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23275 0.0 51.40692 1 GTATCAA 44525 0.0 42.791008 1 ATCAACG 52605 0.0 34.96218 3 CAACGCA 52455 0.0 34.95416 5 TATCAAC 56310 0.0 33.39077 2 AACGCAG 57105 0.0 32.44525 6 GTACTAG 6965 0.0 31.872421 1 TCAACGC 58675 0.0 31.46528 4 GCATCGT 6655 0.0 30.149519 8 ACGCAGA 62795 0.0 29.087244 7 CGCAGAG 63140 0.0 28.838173 8 TAGGCAT 8575 0.0 28.44014 5 GCAGAGT 66905 0.0 26.592556 9 GTACATG 61455 0.0 26.303679 1 GCTATTG 10905 0.0 25.68155 8 TACATGG 64000 0.0 25.013746 2 CTGTGCG 8600 0.0 24.9175 9 CTAGGCA 9610 0.0 24.790611 4 ACATGGG 63115 0.0 24.621038 3 ATTTAGG 11840 0.0 24.350224 1 >>END_MODULE