FastQCFastQC Report
Mon 6 Jun 2016
ERR1211314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10439440
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413190.3957970925643521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334240.32017043059780986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197830.18950250204991836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232550.050.9568021
GTATCAA432050.041.3746261
ATCAACG522550.033.1513673
CAACGCA524300.033.0081755
TATCAAC559350.031.7641832
AACGCAG572700.030.816576
GTACTAG69900.030.3471811
TCAACGC586750.029.740414
ACGCAGA631400.027.409237
TAGGCAT87200.027.330025
CGCAGAG634750.027.2350068
GTACATG601350.025.1374111
GCAGAGT681700.025.0213559
GCTATTG110900.024.9158958
GTGGTAT99050.024.6380311
GCATCGT78650.024.574878
CTAGGCA96450.024.421674
TACATGG624000.024.044732
CTGTGCG86350.024.0126579
ATTTAGG118000.023.9823881
Produced by FastQC (version 0.11.5)