FastQCFastQC Report
Mon 6 Jun 2016
ERR1211313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7734404
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271010.3503954538707831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221870.2868611466378017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118770.1535606363463817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137300.053.4856451
GTATCAA252500.041.5478361
CAACGCA300500.034.0420465
ATCAACG303150.033.759973
TATCAAC317000.032.780322
TCAACGC319900.032.124084
AACGCAG323250.031.9662176
ACGCAGA361500.028.3252097
GTGGTAT61650.027.9288391
CGCAGAG368950.027.6506448
GTACATG334050.026.278771
TACATGG345450.024.8652212
GCAGAGT399750.024.6397029
TGGTATC66200.024.302162
ACATGGG347450.023.883623
GTACTAG30750.022.6423511
GAGTACT218750.022.159412-13
CAGAGTA393700.021.36085110-11
CATGGGG229500.021.0068444
GTACTTT232100.020.76301214-15
Produced by FastQC (version 0.11.5)