##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211313_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7734404 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.029613400075817 31.0 31.0 34.0 27.0 34.0 2 31.044834482398386 31.0 31.0 34.0 27.0 34.0 3 31.079535022995955 31.0 31.0 34.0 27.0 34.0 4 34.67561896171961 37.0 35.0 37.0 31.0 37.0 5 34.72154751678345 37.0 35.0 37.0 31.0 37.0 6 34.7908658508141 37.0 35.0 37.0 31.0 37.0 7 34.7141859928703 37.0 35.0 37.0 30.0 37.0 8 34.71242451260627 37.0 35.0 37.0 30.0 37.0 9 36.206628590903705 39.0 35.0 39.0 30.0 39.0 10-11 36.13283990595785 39.0 35.0 39.0 30.0 39.0 12-13 36.04086358819633 39.0 35.0 39.0 30.0 39.0 14-15 37.10246917538831 40.0 36.0 41.0 30.0 41.0 16-17 37.09650672501721 40.0 36.0 41.0 30.0 41.0 18-19 37.10672994325096 40.0 36.0 41.0 30.0 41.0 20-21 37.078845183158265 40.0 36.0 41.0 30.0 41.0 22-23 36.954272753272264 40.0 36.0 41.0 30.0 41.0 24-25 36.83155444168678 39.5 36.0 41.0 30.0 41.0 26-27 36.67649175036628 39.0 36.0 41.0 29.5 41.0 28-29 36.53792619314947 39.0 36.0 41.0 29.0 41.0 30-31 36.350340310643205 39.0 35.0 40.5 28.5 41.0 32-33 36.224644717291724 39.0 35.0 40.0 28.0 41.0 34-35 36.07334055733318 39.0 35.0 40.0 27.5 41.0 36-37 35.97018483906452 39.0 35.0 40.0 27.0 41.0 38-39 35.817155917896194 38.0 35.0 40.0 27.0 41.0 40-41 35.646678787402365 38.0 34.0 40.0 26.5 41.0 42-43 35.48868038183679 38.0 34.0 40.0 26.0 41.0 44-45 35.34196015620596 38.0 34.0 40.0 25.0 41.0 46-47 35.15864790616058 38.0 33.5 40.0 24.5 41.0 48-49 34.90817437258255 38.0 33.0 40.0 24.0 41.0 50-51 34.253931266067816 37.5 32.5 39.5 23.0 40.5 52-53 34.51653301275702 38.0 33.0 39.5 24.0 40.5 54-55 34.87445580810105 38.0 33.0 40.0 24.0 41.0 56-57 34.77003082590462 38.0 33.0 40.0 24.0 41.0 58-59 34.54389814651523 37.5 33.0 40.0 23.0 41.0 60-61 34.28254025778845 37.0 33.0 40.0 23.0 41.0 62-63 34.01147560949751 37.0 32.5 40.0 23.0 41.0 64-65 33.73499645221533 36.0 32.0 39.5 22.0 41.0 66-67 33.51524170446747 36.0 32.0 39.0 22.0 41.0 68-69 33.17551507524045 35.0 31.5 39.0 22.0 41.0 70-71 32.83967129206077 35.0 31.0 38.5 21.0 40.0 72-73 32.485421436480436 35.0 31.0 37.5 20.0 40.0 74-75 32.11648570465158 35.0 31.0 37.0 20.0 39.5 76-77 31.768998361088975 35.0 30.0 36.5 20.0 39.0 78-79 31.404002881152834 34.5 30.0 36.0 19.0 39.0 80-81 31.069345744028887 34.0 30.0 36.0 18.0 37.5 82-83 30.75765107434264 34.0 29.0 35.0 18.0 37.0 84-85 30.457780134060748 34.0 29.0 35.0 18.0 37.0 86-87 30.204684097184476 34.0 29.0 35.0 17.0 36.0 88-89 29.94452921000765 34.0 29.0 35.0 16.5 36.0 90-91 29.682279849875957 34.0 28.5 35.0 15.0 35.5 92-93 29.441648380405265 34.0 27.5 35.0 14.0 35.0 94-95 29.22180707136581 34.0 27.0 35.0 11.5 35.0 96-97 29.0229647947017 33.5 27.0 35.0 10.5 35.0 98-99 28.78067185267281 33.0 26.0 35.0 10.0 35.0 100 27.09177022560497 31.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33621486768527475 1101 2 -0.3723378383535447 1101 3 -0.36830188311959233 1101 4 -0.2401429262898347 1101 5 -0.26998565491175697 1101 6 -0.24613310560304313 1101 7 -0.3924389795711818 1101 8 -0.47879650228290416 1101 9 -0.4822033621658548 1101 10-11 -0.49469210153610277 1101 12-13 -0.37423981177840204 1101 14-15 -0.44919546817743594 1101 16-17 -0.5478495066007625 1101 18-19 -0.5993542682483266 1101 20-21 -0.7395630565732887 1101 22-23 -0.6796085252107176 1101 24-25 -0.5951929240411005 1101 26-27 -0.7491533883483825 1101 28-29 -0.7117779711061161 1101 30-31 -0.7359608289502546 1101 32-33 -0.816852190680784 1101 34-35 -0.7921401663530574 1101 36-37 -0.808645916575486 1101 38-39 -0.7261819560197509 1101 40-41 -0.7638335997810302 1101 42-43 -0.8531629187402174 1101 44-45 -0.9343048011040338 1101 46-47 -0.8342582122896331 1101 48-49 -0.7608441633889029 1101 50-51 -0.7266254288755292 1101 52-53 -0.5818684215338905 1101 54-55 -0.5967109257239755 1101 56-57 -0.595213268565324 1101 58-59 -0.6280901663583194 1101 60-61 -0.6651265405531817 1101 62-63 -0.7130879141914619 1101 64-65 -0.7165731009531342 1101 66-67 -0.6815807437142993 1101 68-69 -0.6422360792902211 1101 70-71 -0.648623555502482 1101 72-73 -0.7397039312007365 1101 74-75 -0.6964555206574623 1101 76-77 -0.7243581163720023 1101 78-79 -0.6821620897284326 1101 80-81 -0.8539130022217485 1101 82-83 -0.8658461515454086 1101 84-85 -0.8319279205920544 1101 86-87 -0.8520458435875824 1101 88-89 -0.8847514851092448 1101 90-91 -0.9465767468556336 1101 92-93 -0.9442309062962195 1101 94-95 -0.9418976690355976 1101 96-97 -1.1845365362822626 1101 98-99 -1.1899316135386826 1101 100 -1.2361335058847622 1102 1 -0.3544558042187589 1102 2 -0.3687568747092165 1102 3 -0.3895221776401776 1102 4 -0.25889753744527866 1102 5 -0.1600990627926393 1102 6 -0.18437251882687633 1102 7 -0.22282663891065368 1102 8 -0.2269272665566504 1102 9 -0.2627837469073384 1102 10-11 -0.3152896161694372 1102 12-13 -0.36197927748601444 1102 14-15 -0.5088169550963215 1102 16-17 -0.49506238456867635 1102 18-19 -0.48921277778132577 1102 20-21 -0.5819345149913033 1102 22-23 -0.5779075670090421 1102 24-25 -0.6254822195943532 1102 26-27 -0.5942269236180024 1102 28-29 -0.6443109406322449 1102 30-31 -0.5709187466740602 1102 32-33 -0.5889790069244683 1102 34-35 -0.6023831097698249 1102 36-37 -0.6949564342492636 1102 38-39 -0.7571507828393962 1102 40-41 -0.7743901996575104 1102 42-43 -0.6649178015764008 1102 44-45 -0.7018971168313826 1102 46-47 -0.8575626285924685 1102 48-49 -0.7807754239468423 1102 50-51 -0.7750645579339661 1102 52-53 -0.7332501416976172 1102 54-55 -0.584448678367103 1102 56-57 -0.6075944421297024 1102 58-59 -0.6403473215839526 1102 60-61 -0.6872486411633005 1102 62-63 -0.6852176351148103 1102 64-65 -0.786087827057834 1102 66-67 -0.6603202674778572 1102 68-69 -0.6479547121006064 1102 70-71 -0.7751656806241058 1102 72-73 -0.8165492179089995 1102 74-75 -0.8200645122957511 1102 76-77 -0.8713856501750961 1102 78-79 -0.8169851779823496 1102 80-81 -0.84454916584885 1102 82-83 -0.948124798765118 1102 84-85 -0.9601994596106955 1102 86-87 -0.9252670200163564 1102 88-89 -1.0472970488595656 1102 90-91 -1.1984429329506092 1102 92-93 -1.115062817095783 1102 94-95 -1.135768471142839 1102 96-97 -1.2302206905637867 1102 98-99 -1.2108082127314397 1102 100 -1.1436553009594803 1103 1 -0.34147691619434184 1103 2 -0.4136319073065309 1103 3 -0.37301465924482713 1103 4 -0.21704939828622827 1103 5 -0.171707131778561 1103 6 -0.3592226250625643 1103 7 -0.3912157804875207 1103 8 -0.405809702862193 1103 9 -0.4929689673852593 1103 10-11 -0.5402028849926737 1103 12-13 -0.6447991152491426 1103 14-15 -0.7130324882282792 1103 16-17 -0.6903919553432516 1103 18-19 -0.6190873077565371 1103 20-21 -0.7996125078711529 1103 22-23 -0.7577456972917673 1103 24-25 -0.8092078778291807 1103 26-27 -0.7572349683425941 1103 28-29 -0.7755225570064255 1103 30-31 -0.8338455829073155 1103 32-33 -0.8715592909913923 1103 34-35 -0.9208827281464878 1103 36-37 -0.9584633063942078 1103 38-39 -0.9431698179773846 1103 40-41 -0.936455539299196 1103 42-43 -0.9138225010391707 1103 44-45 -0.9543482462609632 1103 46-47 -0.9128501361318015 1103 48-49 -1.0819926930878054 1103 50-51 -0.9177418272843099 1103 52-53 -0.8186892409864939 1103 54-55 -0.843962437281526 1103 56-57 -0.8583041745561744 1103 58-59 -0.8499676587056015 1103 60-61 -0.7923618550598732 1103 62-63 -0.8940084725832946 1103 64-65 -0.8596324474311388 1103 66-67 -0.906384093711317 1103 68-69 -0.8929247772213458 1103 70-71 -0.8805659779485602 1103 72-73 -0.9606428925060584 1103 74-75 -0.9118996125292185 1103 76-77 -0.8746314690211605 1103 78-79 -0.8462211970059421 1103 80-81 -0.874322867367372 1103 82-83 -0.9731404277724316 1103 84-85 -0.9212291206263394 1103 86-87 -1.0562820133952933 1103 88-89 -1.0819105663306523 1103 90-91 -1.0165175162206985 1103 92-93 -1.0627548656911046 1103 94-95 -1.1921114466102622 1103 96-97 -1.3272867167888087 1103 98-99 -1.4125271841680345 1103 100 -1.5052816275200627 1104 1 -0.39760237653846175 1104 2 -0.45825251482172646 1104 3 -0.44636234551015264 1104 4 -0.3419445026883423 1104 5 -0.23894371193728148 1104 6 -0.29348718873878 1104 7 -0.3031023424537693 1104 8 -0.35945338799641036 1104 9 -0.4652872609503689 1104 10-11 -0.42857992792574606 1104 12-13 -0.48890627971737644 1104 14-15 -0.6682982413675091 1104 16-17 -0.6042127361276073 1104 18-19 -0.6975597897236625 1104 20-21 -0.6827375839995327 1104 22-23 -0.6404781957946568 1104 24-25 -0.6899330546917923 1104 26-27 -0.7097403604835293 1104 28-29 -0.783362203136285 1104 30-31 -0.7268516226534771 1104 32-33 -0.8509345428759474 1104 34-35 -0.8066173576563713 1104 36-37 -0.8937893003321449 1104 38-39 -0.7870764931090619 1104 40-41 -0.8351548848008008 1104 42-43 -0.8368965363971981 1104 44-45 -0.8680767721845584 1104 46-47 -0.9568809274399825 1104 48-49 -0.9155656096769675 1104 50-51 -0.8749059234392931 1104 52-53 -0.8228584322103956 1104 54-55 -0.7723680633593162 1104 56-57 -0.7972448122446494 1104 58-59 -0.7931999784169577 1104 60-61 -0.672461594490315 1104 62-63 -0.7214689514312624 1104 64-65 -0.7357201089358867 1104 66-67 -0.8543430503834486 1104 68-69 -0.7196444951654755 1104 70-71 -0.7616081817567988 1104 72-73 -0.7514151739838475 1104 74-75 -0.8067839424409016 1104 76-77 -0.8008536843676239 1104 78-79 -0.7994773960586805 1104 80-81 -0.7702215503415992 1104 82-83 -0.8663179350543189 1104 84-85 -1.0064688912248663 1104 86-87 -1.0340275795416964 1104 88-89 -1.0357704658392386 1104 90-91 -0.9340615668886088 1104 92-93 -0.9214900752453516 1104 94-95 -1.0172127389616286 1104 96-97 -1.0752097193271375 1104 98-99 -1.0099139100428296 1104 100 -1.0570329032455774 1105 1 -0.4437284177600631 1105 2 -0.4899679924292961 1105 3 -0.44923317395691953 1105 4 -0.3620794529396463 1105 5 -0.3607056609912007 1105 6 -0.3771559733562171 1105 7 -0.40569041929546756 1105 8 -0.43109547092216616 1105 9 -0.543303709501906 1105 10-11 -0.5930276108688517 1105 12-13 -0.5842547784456471 1105 14-15 -0.6432531755715445 1105 16-17 -0.7428117002638928 1105 18-19 -0.7735192901912669 1105 20-21 -0.6745424664416433 1105 22-23 -0.7028198452252568 1105 24-25 -0.6609512496100791 1105 26-27 -0.6468318891297784 1105 28-29 -0.764900240582655 1105 30-31 -0.7848321318798739 1105 32-33 -0.7089057429653494 1105 34-35 -0.8588184850290048 1105 36-37 -0.8476545946776781 1105 38-39 -0.8157868777493178 1105 40-41 -1.0076730327338268 1105 42-43 -0.9905031676285816 1105 44-45 -0.917696054855476 1105 46-47 -0.9346434211712094 1105 48-49 -0.9731776021116545 1105 50-51 -0.9261654408841054 1105 52-53 -0.7847388837455398 1105 54-55 -0.7846856145307513 1105 56-57 -0.7293212264861992 1105 58-59 -0.6973363317789065 1105 60-61 -0.7215829799712239 1105 62-63 -0.7985086439112905 1105 64-65 -0.6732290249010546 1105 66-67 -0.7354445254469724 1105 68-69 -0.761287810843676 1105 70-71 -0.7961852079788692 1105 72-73 -0.8166617137297578 1105 74-75 -0.808595141029901 1105 76-77 -0.7367427714216319 1105 78-79 -0.8571398317079293 1105 80-81 -0.842947156251153 1105 82-83 -0.898658668223586 1105 84-85 -0.8554255938718818 1105 86-87 -0.8034502294046391 1105 88-89 -0.8499890834489996 1105 90-91 -0.9560698808368642 1105 92-93 -0.9939657117783511 1105 94-95 -0.927829218732473 1105 96-97 -1.007746882124895 1105 98-99 -1.094913549594608 1105 100 -1.0348131947182715 1106 1 -0.4293968565675428 1106 2 -0.4771848697426364 1106 3 -0.4659585895145 1106 4 -0.38120053492698247 1106 5 -0.3751278838719969 1106 6 -0.3094035175263201 1106 7 -0.3021463539446998 1106 8 -0.37105498705880535 1106 9 -0.41006481396585315 1106 10-11 -0.5044556131253941 1106 12-13 -0.49904793096882827 1106 14-15 -0.5983664626411098 1106 16-17 -0.7230606335277088 1106 18-19 -0.6804971830604813 1106 20-21 -0.6716547659944112 1106 22-23 -0.5767172111671002 1106 24-25 -0.6093390878034626 1106 26-27 -0.634836839222892 1106 28-29 -0.6899190495218832 1106 30-31 -0.725181914142766 1106 32-33 -0.7638969085654352 1106 34-35 -0.8181967878457002 1106 36-37 -0.8787684468776149 1106 38-39 -0.7407866600092845 1106 40-41 -0.7673079414469797 1106 42-43 -0.855423740449119 1106 44-45 -0.7296161905007921 1106 46-47 -0.7386636854322077 1106 48-49 -0.736307866707584 1106 50-51 -0.7895531823366113 1106 52-53 -0.6424777181097951 1106 54-55 -0.8503156749771392 1106 56-57 -0.7232346180943594 1106 58-59 -0.685017594509759 1106 60-61 -0.7342219266074963 1106 62-63 -0.6733911300546964 1106 64-65 -0.689740090741715 1106 66-67 -0.690181721412479 1106 68-69 -0.6786931130735709 1106 70-71 -0.7800418743632065 1106 72-73 -0.6806722793528905 1106 74-75 -0.7588267550470285 1106 76-77 -0.7227528721040386 1106 78-79 -0.655297715969251 1106 80-81 -0.7716199059605344 1106 82-83 -0.7506054716157955 1106 84-85 -0.6531928320579077 1106 86-87 -0.6899949300886696 1106 88-89 -0.817069216515339 1106 90-91 -0.7849211306091135 1106 92-93 -0.7326460505315708 1106 94-95 -0.7839561923266558 1106 96-97 -0.7827284005263841 1106 98-99 -0.7709395303936226 1106 100 -0.9212131640417702 1107 1 -0.45558543501052995 1107 2 -0.5252428681618113 1107 3 -0.4886902865458538 1107 4 -0.4168465397351184 1107 5 -0.32179481316686775 1107 6 -0.3491015811301139 1107 7 -0.34668305912224184 1107 8 -0.320444061416282 1107 9 -0.47156260296469554 1107 10-11 -0.5480666576675546 1107 12-13 -0.5537214714758107 1107 14-15 -0.6399726748243921 1107 16-17 -0.6271727666373437 1107 18-19 -0.5648309317312581 1107 20-21 -0.66084711560476 1107 22-23 -0.628790157055171 1107 24-25 -0.7458321327031072 1107 26-27 -0.6692528214619742 1107 28-29 -0.6700550184801344 1107 30-31 -0.6854290231288971 1107 32-33 -0.7613359062796334 1107 34-35 -0.7776884634185208 1107 36-37 -0.7447108917567249 1107 38-39 -0.7885815491211403 1107 40-41 -0.7991686663921271 1107 42-43 -0.8974830428776812 1107 44-45 -0.8836927876755141 1107 46-47 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40-41 1.186640706732092 2311 42-43 1.1603260984583343 2311 44-45 1.3224435669935914 2311 46-47 1.2227046718932897 2311 48-49 1.174586092547976 2311 50-51 1.2201535214799506 2311 52-53 1.144503291152084 2311 54-55 1.1709252359687454 2311 56-57 1.0836168850737025 2311 58-59 1.0607238910588705 2311 60-61 1.0408052845163809 2311 62-63 1.0582711603205581 2311 64-65 1.0976257320097247 2311 66-67 1.1433306944985517 2311 68-69 1.1252402772506471 2311 70-71 1.0678625179556889 2311 72-73 0.9827759275413115 2311 74-75 1.053664045505485 2311 76-77 1.076736114822495 2311 78-79 1.0725749552116604 2311 80-81 1.1129442485089918 2311 82-83 1.1550422050823848 2311 84-85 1.2551895217038265 2311 86-87 1.2039162718897352 2311 88-89 1.242116300721129 2311 90-91 1.2555378229714123 2311 92-93 1.1806131167358878 2311 94-95 1.247923224249913 2311 96-97 1.2772036067781478 2311 98-99 1.3196728146540124 2311 100 1.408215130101194 2312 1 0.5411904301147068 2312 2 0.5410390222300911 2312 3 0.532911312005762 2312 4 0.4852529009535971 2312 5 0.4712586495630191 2312 6 0.49094896778462527 2312 7 0.5175584867362346 2312 8 0.555175782006728 2312 9 0.6361295363624961 2312 10-11 0.6777043978418433 2312 12-13 0.6834838368403595 2312 14-15 0.849081088641725 2312 16-17 0.8551542183625145 2312 18-19 0.8309032291790786 2312 20-21 0.7856797363267205 2312 22-23 0.8628442250855031 2312 24-25 0.8389996315236345 2312 26-27 0.9071818230314932 2312 28-29 0.9537577643063742 2312 30-31 0.9685628485838436 2312 32-33 0.9184319487135681 2312 34-35 0.988863607324241 2312 36-37 0.9998919778135829 2312 38-39 1.0191102377807724 2312 40-41 1.010556212501875 2312 42-43 1.0592319911706127 2312 44-45 1.0399904602709427 2312 46-47 1.103163228134484 2312 48-49 1.1361353732474697 2312 50-51 1.0607675439016262 2312 52-53 0.946051137906025 2312 54-55 0.9417864245745662 2312 56-57 0.8991001052835017 2312 58-59 0.8968358288736553 2312 60-61 0.9101572914043672 2312 62-63 0.9412632261721257 2312 64-65 0.8855538546087516 2312 66-67 0.8262671522673628 2312 68-69 0.9295601998039231 2312 70-71 0.9371395435206864 2312 72-73 1.0085825468735834 2312 74-75 0.9155468328308132 2312 76-77 0.9182372367049254 2312 78-79 0.9845822840448513 2312 80-81 1.0292587472906298 2312 82-83 1.0307925043508668 2312 84-85 1.012718129942229 2312 86-87 1.085341033675597 2312 88-89 1.1310985966166633 2312 90-91 1.093983153810342 2312 92-93 1.197104738531987 2312 94-95 1.1922518907709296 2312 96-97 1.1624950298080599 2312 98-99 1.2545186557862955 2312 100 1.3011984178309604 2313 1 0.45215923819632664 2313 2 0.5108994408093217 2313 3 0.4913453987336034 2313 4 0.4685873840458896 2313 5 0.44685393939862905 2313 6 0.45150207906311834 2313 7 0.4876926179920318 2313 8 0.47472621668737247 2313 9 0.5851757728682898 2313 10-11 0.6056618683092623 2313 12-13 0.6480286019963657 2313 14-15 0.7663701456289047 2313 16-17 0.7741413266773876 2313 18-19 0.741848358718137 2313 20-21 0.7399459767317893 2313 22-23 0.6545663167966964 2313 24-25 0.6990611106555633 2313 26-27 0.7775741939075829 2313 28-29 0.7881090614189858 2313 30-31 0.8660024959765167 2313 32-33 0.8415757816115317 2313 34-35 0.84364884601829 2313 36-37 0.902547106093877 2313 38-39 0.9161782294344434 2313 40-41 0.9330670672173014 2313 42-43 0.7340414583439596 2313 44-45 0.8709745353747707 2313 46-47 0.9641458384488146 2313 48-49 0.9758151368474657 2313 50-51 0.9204303061030501 2313 52-53 0.7877519701811693 2313 54-55 0.7018756491156282 2313 56-57 0.7432461058444062 2313 58-59 0.7336224410256946 2313 60-61 0.8004449682707744 2313 62-63 0.7264581379915995 2313 64-65 0.7429396584306929 2313 66-67 0.7399751884571941 2313 68-69 0.7873065632513061 2313 70-71 0.7922862387546843 2313 72-73 0.7669040170179215 2313 74-75 0.8267311566515971 2313 76-77 0.7781858968069315 2313 78-79 0.8652689013016079 2313 80-81 0.8417160285347229 2313 82-83 0.8354069474622996 2313 84-85 0.8889562819580625 2313 86-87 0.8718357539756312 2313 88-89 0.845766405627792 2313 90-91 0.9938132320786899 2313 92-93 0.9647307483552012 2313 94-95 0.9795660962153931 2313 96-97 1.0328303184833167 2313 98-99 1.0102060290138404 2313 100 1.0668621068781903 2314 1 0.32663638959396835 2314 2 0.2671494584484364 2314 3 0.32721541336499627 2314 4 0.32012234497575065 2314 5 0.229659747243943 2314 6 0.3225318415942482 2314 7 0.4280526998859102 2314 8 0.39012808312761393 2314 9 0.4973031389793334 2314 10-11 0.5200422031339613 2314 12-13 0.4926978897349059 2314 14-15 0.5987939557344504 2314 16-17 0.5863441201691018 2314 18-19 0.5656344196232865 2314 20-21 0.6534780842574222 2314 22-23 0.6759060061587618 2314 24-25 0.6902311958837402 2314 26-27 0.7120229039942956 2314 28-29 0.6388308590928844 2314 30-31 0.6890718039949988 2314 32-33 0.780918363687853 2314 34-35 0.8545176662295333 2314 36-37 0.7458726207629667 2314 38-39 0.8624613249521786 2314 40-41 0.9166387629673451 2314 42-43 0.7646715442051288 2314 44-45 0.8980649671924326 2314 46-47 0.8048662824465325 2314 48-49 0.6679292020569605 2314 50-51 0.8292189907007312 2314 52-53 0.7004519398015745 2314 54-55 0.7828933318760178 2314 56-57 0.7298298066761291 2314 58-59 0.6894096813325419 2314 60-61 0.54248670302443 2314 62-63 0.7139120772195042 2314 64-65 0.7380426617976568 2314 66-67 0.7892924689852592 2314 68-69 0.7013810610001556 2314 70-71 0.6892676769615349 2314 72-73 0.7536956956287852 2314 74-75 0.8067844156950201 2314 76-77 0.870956220294218 2314 78-79 0.8347111232174207 2314 80-81 0.8554531202295017 2314 82-83 0.9105395406986325 2314 84-85 0.9902129228333187 2314 86-87 0.9503592909066967 2314 88-89 0.7911318009274417 2314 90-91 0.7711504855292048 2314 92-93 1.0187291731317245 2314 94-95 1.0291262673732824 2314 96-97 1.0172838188500108 2314 98-99 1.035625116775197 2314 100 1.0951559295992261 2315 1 0.17443068327832734 2315 2 0.14464113269279366 2315 3 0.1715772409541394 2315 4 0.07597876382817503 2315 5 0.05386820267749215 2315 6 0.0677922332315859 2315 7 0.06385242651678169 2315 8 0.06583512979000972 2315 9 -0.0064604762928510695 2315 10-11 0.10770258306698821 2315 12-13 0.09712039209404821 2315 14-15 0.2075935415852399 2315 16-17 0.08094259911725032 2315 18-19 0.187067051728512 2315 20-21 0.162246757211868 2315 22-23 0.06241888912821736 2315 24-25 0.21834266076139386 2315 26-27 0.3363463049423743 2315 28-29 0.3207324779542873 2315 30-31 0.2907138127500346 2315 32-33 0.3514506004593372 2315 34-35 0.2620283529999412 2315 36-37 0.4281070683091599 2315 38-39 0.5100052401003552 2315 40-41 0.5075481320979307 2315 42-43 0.5108362450514434 2315 44-45 0.4840101217825463 2315 46-47 0.5156156250871717 2315 48-49 0.418568970124646 2315 50-51 0.36848198933228815 2315 52-53 0.14386157105978725 2315 54-55 0.2843388158238369 2315 56-57 0.3074409946962575 2315 58-59 0.3484179167928758 2315 60-61 0.3439359117473444 2315 62-63 0.2942088977544586 2315 64-65 0.38184445937361744 2315 66-67 0.2601544516116405 2315 68-69 0.39563910495926535 2315 70-71 0.3587094254849248 2315 72-73 0.38291829550389167 2315 74-75 0.1148030129969726 2315 76-77 0.4249120757625917 2315 78-79 0.4175610319989964 2315 80-81 0.42868423896632635 2315 82-83 0.4440814639256665 2315 84-85 0.5139667491875386 2315 86-87 0.3820110736507445 2315 88-89 0.37038289769580857 2315 90-91 0.4899991612783481 2315 92-93 0.5809535814510767 2315 94-95 0.5762401439246325 2315 96-97 0.5700766338210492 2315 98-99 0.6654581119925069 2315 100 0.6876092764800923 2316 1 -0.11785676547302515 2316 2 -0.10007192769593587 2316 3 -0.13401529322984018 2316 4 -0.23974882633297057 2316 5 -0.22615172083605728 2316 6 -0.3477793974731256 2316 7 -0.3040454193024402 2316 8 -0.23636976851582858 2316 9 -0.18182254169709466 2316 10-11 -0.18278991970405656 2316 12-13 -0.24883518623185807 2316 14-15 -0.3551628735675365 2316 16-17 -0.18727362503798872 2316 18-19 -0.09508119031368523 2316 20-21 -0.06785389681086684 2316 22-23 -0.11310720387126594 2316 24-25 -0.21074119900673338 2316 26-27 -0.2849519482380387 2316 28-29 -0.2519577440754901 2316 30-31 -0.8805320405090598 2316 32-33 -0.6214186556582675 2316 34-35 -0.6886722979281714 2316 36-37 -0.4043396703084454 2316 38-39 -0.3867258619730194 2316 40-41 -0.2182834817926249 2316 42-43 -0.15136188436837728 2316 44-45 -0.26790266568941945 2316 46-47 -0.41831633876658003 2316 48-49 -0.37833825561205714 2316 50-51 -0.22130046810244153 2316 52-53 -0.5604726895978374 2316 54-55 -0.43662545079462944 2316 56-57 -0.43489534181856726 2316 58-59 -0.7510226799694166 2316 60-61 -0.3108158044820968 2316 62-63 -0.3416725593047403 2316 64-65 -0.6554570146765712 2316 66-67 -0.9318228588663047 2316 68-69 -0.8975160432623923 2316 70-71 -0.6625149864189837 2316 72-73 -0.8821764062709292 2316 74-75 -0.6272076015790766 2316 76-77 -0.3543140509585214 2316 78-79 -0.35719128105166575 2316 80-81 -0.40851047039770094 2316 82-83 -0.09634646917435319 2316 84-85 -0.10722577084957052 2316 86-87 -0.004697889547983891 2316 88-89 0.004020122737440346 2316 90-91 -0.5825671528053284 2316 92-93 -0.622280808910773 2316 94-95 -0.20567092922133767 2316 96-97 0.050234010420890485 2316 98-99 0.0275504223071259 2316 100 0.004421853247141172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 223.0 1 582.0 2 364.0 3 313.0 4 281.0 5 297.0 6 325.0 7 641.0 8 1607.0 9 3393.0 10 6607.0 11 13266.0 12 21691.0 13 27778.0 14 31944.0 15 35824.0 16 39775.0 17 45905.0 18 53463.0 19 62415.0 20 73065.0 21 85969.0 22 95294.0 23 96360.0 24 97413.0 25 100906.0 26 111192.0 27 125370.0 28 143580.0 29 169426.0 30 201158.0 31 244012.0 32 303207.0 33 386891.0 34 519846.0 35 705508.0 36 1013289.0 37 1396432.0 38 1238436.0 39 280120.0 40 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.548219598480934 19.627437071485705 13.802571910219164 23.021771419814197 2 15.644170151241665 20.027855027010443 40.65234091601877 23.675633905729114 3 18.907055872721283 25.87388252747031 29.51831845438995 25.700743145418457 4 12.751082046752696 16.76441187994874 38.41058420825989 32.07392186503867 5 13.459755354766626 37.70044084765862 34.56770677746698 14.272097020107774 6 30.71856069773377 37.10196683239646 17.940999296714452 14.238473173155317 7 27.48747859201424 32.45978403158292 22.16443144011714 17.888305936285693 8 25.99032949135215 35.08193468636295 20.121295904552653 18.806439917732256 9 26.381512764593083 16.102803262069862 20.864347113949727 36.65133685938732 10-11 25.022492372123946 26.308334335724847 27.758232221131447 20.910941071019764 12-13 25.58897631613444 24.033031318406614 28.03320773790943 22.344784627549522 14-15 23.15934394595954 25.416170621127694 25.821254179877485 25.60323125303528 16-17 22.155318413068205 28.334922934727523 26.73808490928925 22.77167374291502 18-19 21.824403549533365 27.49913131924427 28.99352736640246 21.682937764819908 20-21 22.891223632190552 26.65523778897655 28.882057979283793 21.57148059954911 22-23 22.97999908287979 26.431650193536292 28.52463751599867 22.063713207585245 24-25 22.49907041385408 26.603157668936916 28.8923330943277 22.005438822881302 26-27 22.372742712808726 26.848542244793794 28.561239910340152 22.21747513205733 28-29 22.070859455338525 26.791625840674786 28.916269854949988 22.221244849036697 30-31 22.538102905786374 26.675558983184324 28.764828648169544 22.021509462859758 32-33 22.503848963471953 26.910866058011422 28.391351546424758 22.193933432091868 34-35 22.648632420302775 26.89963398752329 28.61161155697072 21.84012203520322 36-37 22.59820867475549 26.69475716167606 28.42911197461042 22.277922188958033 38-39 22.503385975461356 26.816071924385948 28.62963278440464 22.050909315748058 40-41 22.496086854969814 26.455512397064673 28.867014905688336 22.18138584227718 42-43 22.369532326014156 26.52971262121378 28.534902925806044 22.56585212696602 44-45 22.408726262230314 26.935458622960322 28.333048719431495 22.322766395377872 46-47 22.42865211958815 26.833611870738977 28.033230948520128 22.704505061152737 48-49 22.637297454639544 26.778873831624566 28.032400789158768 22.551427924577123 50-51 22.58072507421553 26.908808686153556 27.818767150294406 22.69169908933651 52-53 22.397120619346293 27.18070131859488 27.719233217412903 22.702944844645927 54-55 22.392840624349773 27.14656536411941 27.711541281286035 22.749052730244777 56-57 22.587280894239658 27.007063582592426 27.56081792800068 22.84483759516723 58-59 22.298812569337507 26.981196341049458 27.9389917912672 22.780999298345836 60-61 22.473126047814592 27.22357243518304 27.63438398995896 22.668917527043405 62-63 22.50373748493153 27.05682303075957 27.623398127185805 22.816041357123098 64-65 22.223349108592636 26.91795916508759 27.72487376860354 23.133817957716236 66-67 22.395450072858367 26.817356829544416 27.60689456360624 23.180298533990985 68-69 22.16193612089533 26.91287216643483 27.729140746255577 23.196050966414262 70-71 22.280442896559904 26.63969935743013 27.777749389018197 23.30210835699177 72-73 22.424914753588304 26.84936442384163 27.637839154259865 23.0878816683102 74-75 22.40069578711046 27.051686201668957 27.521253244685628 23.026364766534954 76-77 22.30223388746092 26.92535512071064 27.472850939205507 23.299560052622933 78-79 22.200720564285266 26.776828379963273 27.572137447860584 23.450313607890877 80-81 22.14819806203639 27.074016750007456 27.52800481421536 23.24978037374079 82-83 22.471119207243106 26.75024793218446 27.831111306011604 22.94752155456083 84-85 22.461246072666498 26.81023449220329 27.485762051633266 23.242757383496947 86-87 22.0761445002585 27.001144805880728 27.72800824927752 23.19470244458325 88-89 22.313888563295702 26.496672092644758 27.765366424295962 23.424072919763574 90-91 22.391475286605125 26.552001974367506 27.742593453341495 23.313929285685866 92-93 22.41218210849371 26.635019169189146 27.6596121833765 23.29318653894065 94-95 21.974486071254535 26.47009616213227 27.881189212885555 23.674228553727637 96-97 22.214132653982116 26.39590391533737 27.73674877290904 23.653214657771475 98-99 21.97965204676471 26.51206966828631 27.689818259457432 23.818460025491554 100 22.37056545903342 26.266531322468257 27.304811714099923 24.05809150439841 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1774.0 1 1266.5 2 1089.5 3 1441.5 4 1234.0 5 1110.0 6 1669.5 7 2982.0 8 4933.0 9 6244.0 10 5735.5 11 4912.5 12 5069.0 13 5701.5 14 6373.5 15 7044.0 16 7709.0 17 8443.0 18 9353.0 19 10123.5 20 11054.5 21 12307.5 22 13901.5 23 16258.5 24 19037.5 25 22721.0 26 28243.0 27 34787.0 28 41964.0 29 52899.5 30 68339.0 31 84056.5 32 104270.0 33 129704.0 34 154938.5 35 177909.5 36 203956.5 37 233552.5 38 249305.5 39 252572.5 40 249843.0 41 245563.0 42 246566.5 43 254015.5 44 265146.5 45 281680.5 46 296357.0 47 305146.5 48 312567.5 49 313630.5 50 306684.0 51 298975.0 52 289488.5 53 278956.5 54 269040.5 55 248152.5 56 223669.5 57 195421.0 58 167450.5 59 146773.0 60 123007.0 61 98143.0 62 78062.5 63 61403.0 64 45860.0 65 32661.0 66 23687.5 67 17554.5 68 13340.0 69 10307.5 70 7996.0 71 6001.5 72 4581.5 73 3451.5 74 2516.5 75 1789.5 76 1230.5 77 790.0 78 503.5 79 344.0 80 230.0 81 159.0 82 116.5 83 91.5 84 70.0 85 56.5 86 43.0 87 35.5 88 30.0 89 28.0 90 26.5 91 17.5 92 9.0 93 4.0 94 7.5 95 7.5 96 4.0 97 4.5 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026104144546884284 2 0.11234220503609586 3 0.01717003663113538 4 0.017764781875888563 5 0.020350630766119794 6 0.0458600300682509 7 0.04711416678001304 8 0.03960227575389132 9 0.03395219592873607 10-11 0.03741723344164592 12-13 0.03662208490789982 14-15 0.03127584232734675 16-17 0.037572384375059796 18-19 0.03688066979692295 20-21 0.047767093624796426 22-23 0.047624871935833715 24-25 0.06668904287906346 26-27 0.058472508030353726 28-29 0.05084425380417159 30-31 0.048613959136347154 32-33 0.04430205611188658 34-35 0.04551094046806968 36-37 0.041380046865925287 38-39 0.04678447104650856 40-41 0.056481404384875686 42-43 0.05707614962962886 44-45 0.059914118786657644 46-47 0.0613104771873825 48-49 0.06017270367568076 50-51 0.05450969460607436 52-53 0.06418076945553917 54-55 0.05039173024838113 56-57 0.055097975228601964 58-59 0.053009902249740254 60-61 0.048232546425038055 62-63 0.05524019691756468 64-65 0.05373393993900499 66-67 0.05164586696014328 68-69 0.057037361896275396 70-71 0.05724422980749389 72-73 0.05955856456425085 74-75 0.05014607460380917 76-77 0.050863647670848326 78-79 0.061640172920886985 80-81 0.06200219176551936 82-83 0.05124506038215743 84-85 0.057476956207614706 86-87 0.05524019691756468 88-89 0.06363774118859061 90-91 0.06912620545810641 92-93 0.0740263891050946 94-95 0.07754960821803464 96-97 0.07314720048241596 98-99 0.08215241924264624 100 0.12801244931089714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7734404.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.61193039494693 #Duplication Level Percentage of deduplicated Percentage of total 1 74.09079891541384 42.685139500208315 2 14.691606148222105 16.928235816026927 3 5.206976490526903 8.99951901421083 4 2.3028545556174294 5.306875854716712 5 1.1809174520951466 3.4017467026141834 6 0.6701072545150161 2.316370350256086 7 0.41883282114482856 1.6890837137240586 8 0.29118034454935954 1.3420369394043496 9 0.19745680399982776 1.0238280883242081 >10 0.869285272135151 8.568257528515051 >50 0.04752417590576411 1.872626476579917 >100 0.02958960906108781 3.280706142137117 >500 0.0020060305414474127 0.7871097210105492 >1k 7.406796618286217E-4 0.6133564460621893 >5k 3.086165256063368E-5 0.0975943859240829 >10k+ 9.258495768190103E-5 1.0875133202853946 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27101 0.3503954538707831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22187 0.2868611466378017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11877 0.1535606363463817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 2 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 3 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 4 0.0 0.0 0.0 1.2929244451156158E-5 5.1716977804624633E-5 5 0.0 1.2929244451156158E-5 0.0 2.5858488902312317E-5 5.1716977804624633E-5 6 0.0 1.2929244451156158E-5 0.0 2.5858488902312317E-5 1.0343395560924927E-4 7 0.0 1.2929244451156158E-5 0.0 2.5858488902312317E-5 1.0343395560924927E-4 8 0.0 1.2929244451156158E-5 0.0 2.5858488902312317E-5 1.1636320006040543E-4 9 0.0 1.2929244451156158E-5 0.0 5.1716977804624633E-5 1.551509334138739E-4 10-11 0.0 1.2929244451156158E-5 0.0 5.1716977804624633E-5 1.551509334138739E-4 12-13 0.0 1.2929244451156158E-5 0.0 5.1716977804624633E-5 1.6808017786503005E-4 14-15 0.0 1.2929244451156158E-5 0.0 5.1716977804624633E-5 2.1333253344407661E-4 16-17 0.0 1.2929244451156158E-5 0.0 5.818160003020272E-5 2.585848890231232E-4 18-19 0.0 1.2929244451156158E-5 0.0 6.46462222557808E-5 2.844433779254355E-4 20-21 0.0 1.2929244451156158E-5 0.0 9.69693333836712E-5 2.9090800015101363E-4 22-23 0.0 1.2929244451156158E-5 0.0 3.103018668277478E-4 3.555542224067944E-4 24-25 0.0 1.2929244451156158E-5 0.0 7.628254226182134E-4 4.4605893356488744E-4 26-27 0.0 1.2929244451156158E-5 0.0 0.0017001956453270347 4.7838204469277787E-4 28-29 0.0 1.2929244451156158E-5 0.0 0.00471270960244642 4.84846666918356E-4 30-31 0.0 3.2323111127890393E-5 0.0 0.014493683029746053 5.042405335950902E-4 32-33 0.0 5.1716977804624633E-5 0.0 0.03924672153148452 5.68886755850871E-4 34-35 0.0 5.818160003020272E-5 0.0 0.0787455633297666 6.206037336554956E-4 36-37 0.0 6.46462222557808E-5 0.0 0.1417950238958296 6.335329781066517E-4 38-39 0.0 6.46462222557808E-5 0.0 0.2318019591425532 6.593914670089641E-4 40-41 0.0 6.46462222557808E-5 0.0 0.3277110944812296 6.852499559112764E-4 42-43 0.0 6.46462222557808E-5 0.0 0.42351033124207116 6.852499559112764E-4 44-45 0.0 6.46462222557808E-5 0.0 0.5424141278371287 6.917145781368545E-4 46-47 6.464622225578079E-6 6.46462222557808E-5 0.0 0.6858253072893529 7.046438225880106E-4 48-49 1.2929244451156158E-5 6.46462222557808E-5 0.0 0.8380348375905888 7.822192892949476E-4 50-51 1.2929244451156158E-5 6.46462222557808E-5 0.0 0.9942847567828109 7.886839115205257E-4 52-53 1.2929244451156158E-5 6.46462222557808E-5 0.0 1.14647489321737 8.404008893251504E-4 54-55 1.2929244451156158E-5 6.46462222557808E-5 0.0 1.3158997125053205 8.92117867129775E-4 56-57 1.2929244451156158E-5 6.46462222557808E-5 0.0 1.518138954210305 8.985824893553531E-4 58-59 1.2929244451156158E-5 6.46462222557808E-5 0.0 1.7425712440156993 9.050471115809311E-4 60-61 1.2929244451156158E-5 6.46462222557808E-5 0.0 1.9734487621799948 9.050471115809311E-4 62-63 1.2929244451156158E-5 6.46462222557808E-5 0.0 2.203414768610484 9.179763560320873E-4 64-65 1.2929244451156158E-5 6.46462222557808E-5 0.0 2.4417589254453222 9.179763560320873E-4 66-67 1.2929244451156158E-5 6.46462222557808E-5 0.0 2.71230983020799 9.179763560320873E-4 68-69 1.2929244451156158E-5 6.46462222557808E-5 0.0 3.0105487119628096 9.244409782576653E-4 70-71 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 3.3293127692838387 0.0010278749338669146 72-73 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 3.6478117770936196 0.001150702756152898 74-75 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 3.9650178604582846 0.001150702756152898 76-77 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 4.3100088901484845 0.0011571673783784763 78-79 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 4.69107251185741 0.0011636320006040543 80-81 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 5.093870193488729 0.0011894904895063665 82-83 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 5.502033511567278 0.0012799952006644598 84-85 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 5.899252224217923 0.0012799952006644598 86-87 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 6.314526626744607 0.001299389067341194 88 1.2929244451156158E-5 6.46462222557808E-5 1.2929244451156158E-5 6.6449851856717075 0.001305853689566772 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13730 0.0 53.485645 1 GTATCAA 25250 0.0 41.547836 1 CAACGCA 30050 0.0 34.042046 5 ATCAACG 30315 0.0 33.75997 3 TATCAAC 31700 0.0 32.78032 2 TCAACGC 31990 0.0 32.12408 4 AACGCAG 32325 0.0 31.966217 6 ACGCAGA 36150 0.0 28.325209 7 GTGGTAT 6165 0.0 27.928839 1 CGCAGAG 36895 0.0 27.650644 8 GTACATG 33405 0.0 26.27877 1 TACATGG 34545 0.0 24.865221 2 GCAGAGT 39975 0.0 24.639702 9 TGGTATC 6620 0.0 24.30216 2 ACATGGG 34745 0.0 23.88362 3 GTACTAG 3075 0.0 22.642351 1 GAGTACT 21875 0.0 22.1594 12-13 CAGAGTA 39370 0.0 21.360851 10-11 CATGGGG 22950 0.0 21.006844 4 GTACTTT 23210 0.0 20.763012 14-15 >>END_MODULE