FastQCFastQC Report
Mon 6 Jun 2016
ERR1211313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7734404
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245250.3170897201646048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214270.277034920854923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115710.14960428754432792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141550.050.7621
GTATCAA255600.041.020011
CAACGCA314000.032.4506875
ATCAACG314400.032.403493
TATCAAC329400.031.4513552
TCAACGC332750.030.687264
AACGCAG339000.030.3343416
GTGGTAT67400.027.85311
ACGCAGA378950.026.7526347
CGCAGAG386100.026.2086148
GTACTAG31400.025.2598341
TGGTATC70550.025.2061272
GCAGAGT420450.023.8654969
GTACATG335950.023.6792811
TACATGG344850.022.8446432
TACCTGG47450.022.347872
GAGTACT225350.022.1148312-13
ACATGGG347150.021.6821163
GTACTTT238900.020.732914-15
CAGAGTA419700.020.09397110-11
Produced by FastQC (version 0.11.5)