##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211313_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7734404 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10785136126843 33.0 31.0 34.0 30.0 34.0 2 32.242903784182985 34.0 31.0 34.0 30.0 34.0 3 32.30425279051883 34.0 31.0 34.0 30.0 34.0 4 35.94844399128879 37.0 35.0 37.0 35.0 37.0 5 35.85249749043365 37.0 35.0 37.0 35.0 37.0 6 35.86663148188277 37.0 35.0 37.0 35.0 37.0 7 35.78205288991886 37.0 35.0 37.0 33.0 37.0 8 35.761286066773856 37.0 35.0 37.0 33.0 37.0 9 37.37287992714112 39.0 37.0 39.0 34.0 39.0 10-11 37.3247676873357 39.0 37.0 39.0 34.0 39.0 12-13 37.25863434338315 39.0 37.0 39.0 34.0 39.0 14-15 38.492028021810086 40.0 38.0 41.0 34.0 41.0 16-17 38.47568119017315 40.0 38.0 41.0 34.0 41.0 18-19 38.48500899358244 40.0 38.0 41.0 34.0 41.0 20-21 38.45704310248081 40.0 38.0 41.0 34.0 41.0 22-23 38.35326451786072 40.0 38.0 41.0 34.0 41.0 24-25 38.2644476549195 40.0 38.0 41.0 34.0 41.0 26-27 38.16446593687115 40.0 38.0 41.0 33.0 41.0 28-29 38.0668018505369 40.0 37.5 41.0 33.0 41.0 30-31 38.070416091530774 40.0 37.0 41.0 33.0 41.0 32-33 38.004038966674095 40.0 37.0 41.0 33.0 41.0 34-35 37.8804163837317 40.0 37.0 41.0 33.0 41.0 36-37 37.73621593855195 40.0 37.0 41.0 33.0 41.0 38-39 37.621854056240146 40.0 37.0 41.0 33.0 41.0 40-41 37.51224496418858 40.0 36.5 41.0 32.0 41.0 42-43 37.40408407680799 40.0 36.0 41.0 32.0 41.0 44-45 37.23949847977944 40.0 36.0 41.0 31.0 41.0 46-47 36.99506529527033 39.0 36.0 41.0 31.0 41.0 48-49 35.44355156518847 39.0 35.0 41.0 24.0 41.0 50-51 36.16633854399124 39.0 35.0 40.0 29.0 41.0 52-53 36.25615005370808 39.0 35.0 40.0 29.5 41.0 54-55 36.087656463251726 38.5 35.0 40.0 29.0 41.0 56-57 35.87493625882486 38.0 35.0 40.0 28.5 41.0 58-59 35.637626829423446 38.0 34.0 40.0 28.0 41.0 60-61 35.38426328647948 37.5 34.0 40.0 28.0 41.0 62-63 35.066643014768815 37.0 34.0 40.0 27.5 41.0 64-65 34.7345711447191 36.5 34.0 39.5 27.0 41.0 66-67 34.402610207586775 36.0 33.0 39.0 26.0 40.5 68-69 34.046145637078176 35.5 33.0 39.0 26.0 40.0 70-71 33.69652238233223 35.0 33.0 38.0 26.0 40.0 72-73 33.32187741938487 35.0 32.0 37.5 26.0 39.5 74-75 32.9629102643203 35.0 32.0 37.0 25.0 39.0 76-77 32.0350721270831 34.0 30.5 36.0 24.5 38.5 78-79 32.46845665160496 35.0 32.0 36.0 25.0 38.0 80-81 32.34376165248156 35.0 32.0 36.0 25.0 37.0 82-83 32.07632113605651 34.0 31.5 35.0 25.0 37.0 84-85 31.79795695957956 34.0 31.0 35.0 24.0 36.5 86-87 31.54382102357208 34.0 31.0 35.0 24.0 36.0 88-89 31.31851426431823 34.0 31.0 35.0 23.5 36.0 90-91 31.100997504138654 34.0 31.0 35.0 23.0 35.0 92-93 30.91178020956754 34.0 30.0 35.0 22.0 35.0 94-95 30.71915877422488 34.0 30.0 35.0 20.0 35.0 96-97 30.53320579064657 34.0 30.0 35.0 20.0 35.0 98-99 30.323885654279245 34.0 29.0 35.0 19.5 35.0 100 27.878019560395344 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.27237419644750105 1101 2 -0.25810012879289346 1101 3 -0.31802119240542126 1101 4 -0.1515129322737394 1101 5 -0.1807100073688943 1101 6 -0.24176288276377988 1101 7 -0.23945054610753402 1101 8 -0.20487221468933114 1101 9 -0.37427257664308655 1101 10-11 -0.31754922888170256 1101 12-13 -0.4243163195337658 1101 14-15 -0.5587390761320066 1101 16-17 -0.48464774220699525 1101 18-19 -0.5028098848295457 1101 20-21 -0.5895802749359547 1101 22-23 -0.6202247787460209 1101 24-25 -0.5769660379171526 1101 26-27 -0.6358206891720073 1101 28-29 -0.6177958351987058 1101 30-31 -0.5113427830501394 1101 32-33 -0.46818555057181754 1101 34-35 -0.5483714198171015 1101 36-37 -0.6347042757196064 1101 38-39 -0.6249084943499028 1101 40-41 -0.6175712932436923 1101 42-43 -0.6569173185100965 1101 44-45 -0.60128188393481 1101 46-47 -0.607086524643222 1101 48-49 0.6109316345695888 1101 50-51 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1105 68-69 -0.7502807378758263 1105 70-71 -0.8302258997929002 1105 72-73 -0.7832578899088958 1105 74-75 -0.8370508446050167 1105 76-77 -0.7438744329785578 1105 78-79 -0.7194962689380944 1105 80-81 -0.6918135952633016 1105 82-83 -0.6379567260570198 1105 84-85 -0.6448472196163202 1105 86-87 -0.682664507546427 1105 88-89 -0.6653169482839836 1105 90-91 -0.7269993896673341 1105 92-93 -0.6754497122219014 1105 94-95 -0.6550082779907562 1105 96-97 -0.6754445026370597 1105 98-99 -0.7296065611665483 1105 100 -0.8911051972667998 1106 1 -0.3043337758179021 1106 2 -0.27328289458931465 1106 3 -0.2717869093594061 1106 4 -0.23378378840726555 1106 5 -0.22262659126082696 1106 6 -0.31027793332118847 1106 7 -0.3064174048207562 1106 8 -0.3379631573801305 1106 9 -0.44080147297923844 1106 10-11 -0.42468545533848356 1106 12-13 -0.4196147775573138 1106 14-15 -0.5517805337744335 1106 16-17 -0.5147962892550524 1106 18-19 -0.571644818735578 1106 20-21 -0.5736873440312351 1106 22-23 -0.6247140173042425 1106 24-25 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2310 100 1.2898705665815804 2311 1 0.31062530984985415 2311 2 0.350854089007818 2311 3 0.3808487840543009 2311 4 0.269420601621988 2311 5 0.30918175507439116 2311 6 0.36602196183638114 2311 7 0.3593525021101769 2311 8 0.13675647232154375 2311 9 0.4478046902320969 2311 10-11 0.3457392180943657 2311 12-13 0.46656929739820185 2311 14-15 0.47089748719906055 2311 16-17 0.4656996314333455 2311 18-19 0.6177827481098888 2311 20-21 0.6678195576949051 2311 22-23 0.6769321378414048 2311 24-25 0.6063770279280547 2311 26-27 0.6692892600291671 2311 28-29 0.78715802899994 2311 30-31 0.6050875883742037 2311 32-33 0.6669201896624344 2311 34-35 0.8069083989803048 2311 36-37 0.7999735894106976 2311 38-39 0.8277623677558736 2311 40-41 0.7392764056591972 2311 42-43 0.8155383576956154 2311 44-45 0.8508939838072394 2311 46-47 0.9018896899495203 2311 48-49 -5.997843324015562 2311 50-51 -0.9327437803007399 2311 52-53 0.503870238465538 2311 54-55 0.9417808864939161 2311 56-57 1.0146831515243306 2311 58-59 1.0247576316821352 2311 60-61 0.8323711153557483 2311 62-63 0.683523928764437 2311 64-65 0.8328693686479838 2311 66-67 0.9363415946336389 2311 68-69 0.9514874245756744 2311 70-71 0.9569217691781731 2311 72-73 0.43676141214758957 2311 74-75 0.7815748447575643 2311 76-77 0.8635672148966762 2311 78-79 0.736794364008027 2311 80-81 0.7744759206390555 2311 82-83 0.8041009394594525 2311 84-85 0.8757448499860452 2311 86-87 0.9127625729667557 2311 88-89 0.9597099000450378 2311 90-91 0.9175985732510306 2311 92-93 0.9086403799447496 2311 94-95 1.0006072476147416 2311 96-97 1.0390729277484105 2311 98-99 1.0191046232389844 2311 100 1.3692128957957834 2312 1 0.05394679644942357 2312 2 0.23280111909062384 2312 3 0.19722388529484647 2312 4 -0.057349764161813255 2312 5 0.1638374489311687 2312 6 0.09689344868291272 2312 7 0.2635198820714919 2312 8 0.3123526684197202 2312 9 0.28661068806535894 2312 10-11 0.24612023828803586 2312 12-13 0.26677831768813576 2312 14-15 0.27993537436304194 2312 16-17 0.509558897532763 2312 18-19 0.39699291261376857 2312 20-21 0.37729192931053745 2312 22-23 0.5941389117392433 2312 24-25 0.69735296528129 2312 26-27 0.6131275529014815 2312 28-29 0.7384413971336414 2312 30-31 0.769969100544138 2312 32-33 0.7530161670169164 2312 34-35 0.3643503452033059 2312 36-37 0.6749493137476179 2312 38-39 0.7386859090853974 2312 40-41 0.5264773092603292 2312 42-43 0.578450645941686 2312 44-45 0.676350416190644 2312 46-47 0.7644072438286216 2312 48-49 -4.760039953103295 2312 50-51 -0.8482128344525961 2312 52-53 0.42658434324581407 2312 54-55 0.7183193750322872 2312 56-57 0.8206481968814572 2312 58-59 0.8166797708031197 2312 60-61 0.8860845010089164 2312 62-63 0.6821648988535571 2312 64-65 0.8259368146369965 2312 66-67 0.828155932996232 2312 68-69 0.7612683335636916 2312 70-71 0.7784146084355541 2312 72-73 0.922680555483609 2312 74-75 0.5688375672279946 2312 76-77 0.7605239084798967 2312 78-79 0.45094753797742015 2312 80-81 0.5961443775045083 2312 82-83 0.6198063056315632 2312 84-85 0.6932199225056515 2312 86-87 0.778361874983645 2312 88-89 0.7823759447923386 2312 90-91 0.6218273622327786 2312 92-93 0.8120334214257596 2312 94-95 0.9608645323366396 2312 96-97 0.9824028372674398 2312 98-99 0.9988160013683753 2312 100 0.5431388001666946 2313 1 0.04212823107798869 2313 2 0.20200304867658048 2313 3 0.29261138208133275 2313 4 0.08898642214547436 2313 5 0.16783720066523955 2313 6 0.29449975303849385 2313 7 0.12792375989923244 2313 8 0.23064788408306214 2313 9 0.4316290507238705 2313 10-11 0.21075778284161828 2313 12-13 0.07129092256563041 2313 14-15 0.18932396005497054 2313 16-17 0.08770937631084053 2313 18-19 0.4464017665231026 2313 20-21 0.39955598647197377 2313 22-23 0.48323736968184505 2313 24-25 0.2751117358749582 2313 26-27 0.4034265360157079 2313 28-29 0.38502852730248094 2313 30-31 0.548308548955049 2313 32-33 0.41407860015834785 2313 34-35 0.3202387323439879 2313 36-37 -0.3890135794135645 2313 38-39 0.129928107979417 2313 40-41 0.36803974762948855 2313 42-43 0.4738555531197477 2313 44-45 0.4706514958296779 2313 46-47 0.38406760434929055 2313 48-49 -5.43697082805722 2313 50-51 -1.3784757031767043 2313 52-53 0.10817039660474137 2313 54-55 0.2456419786715216 2313 56-57 0.30354813771585754 2313 58-59 0.4257397936534275 2313 60-61 0.6622940081385167 2313 62-63 0.760561221893667 2313 64-65 0.9089224441192698 2313 66-67 0.8859067579525615 2313 68-69 0.8972950387570222 2313 70-71 0.8880059249912193 2313 72-73 0.6404565063462258 2313 74-75 0.7641885288433272 2313 76-77 0.7604647890018725 2313 78-79 0.7205897090722075 2313 80-81 -0.19939650703563672 2313 82-83 0.28206972008970155 2313 84-85 0.6278829435309667 2313 86-87 0.7453482207674469 2313 88-89 0.7799502523640989 2313 90-91 0.7917091940048309 2313 92-93 0.8435514707279737 2313 94-95 0.7648690223418306 2313 96-97 0.8721059052685263 2313 98-99 0.9219739670761093 2313 100 1.013948871809152 2314 1 0.10899660859781335 2314 2 0.22500710204468533 2314 3 0.2022016133170439 2314 4 0.07639987960173755 2314 5 0.20397765822592362 2314 6 0.30109572414340136 2314 7 0.10403830509427081 2314 8 0.270172128897066 2314 9 0.4304038881113783 2314 10-11 0.336989912905004 2314 12-13 0.202797979925613 2314 14-15 0.24353048860433546 2314 16-17 0.4866819053528886 2314 18-19 0.54944740435959 2314 20-21 0.5930723866166332 2314 22-23 0.3830931900660488 2314 24-25 0.470215454886052 2314 26-27 0.525607805249912 2314 28-29 0.6555826712247637 2314 30-31 0.570402625518085 2314 32-33 0.5595855468454829 2314 34-35 0.3976658596113225 2314 36-37 0.4642019861085913 2314 38-39 0.5004479236617385 2314 40-41 0.27884496118777946 2314 42-43 0.32167392707143705 2314 44-45 0.5592512266870031 2314 46-47 0.6970342849659801 2314 48-49 -6.524616358325339 2314 50-51 -1.5384709033212047 2314 52-53 -0.7311707945012742 2314 54-55 -0.35767664968660995 2314 56-57 -0.033623820080897815 2314 58-59 0.44372810133847906 2314 60-61 0.6582923730428476 2314 62-63 0.5268959002348055 2314 64-65 0.7340965522754743 2314 66-67 0.756309336509112 2314 68-69 0.7658457621018968 2314 70-71 0.5628479188282185 2314 72-73 0.7537304628866721 2314 74-75 0.7832751738955253 2314 76-77 0.7716479130647258 2314 78-79 0.542636679115553 2314 80-81 0.6121952800709138 2314 82-83 0.5548195433961567 2314 84-85 0.5694073708846155 2314 86-87 0.6507570743135886 2314 88-89 0.561275483194617 2314 90-91 0.7637819121180449 2314 92-93 0.8148355565560834 2314 94-95 0.826095188368047 2314 96-97 0.9576845217006031 2314 98-99 0.9449116343705235 2314 100 -0.39089437087726964 2315 1 0.07937004106341305 2315 2 0.17015791015168702 2315 3 0.26758816161896704 2315 4 0.14781523890220427 2315 5 0.21458831582016558 2315 6 0.2623155404644635 2315 7 0.32741179881836047 2315 8 0.2630704483093922 2315 9 0.42863157194371126 2315 10-11 0.217624692710082 2315 12-13 0.3757869111480474 2315 14-15 0.2258642311404202 2315 16-17 0.36342149599985873 2315 18-19 0.4365924789503879 2315 20-21 0.322758590363172 2315 22-23 0.3664589217270873 2315 24-25 0.4029057208519191 2315 26-27 0.425836133714661 2315 28-29 0.5562657730821172 2315 30-31 0.5326946271843553 2315 32-33 0.6145547924449062 2315 34-35 0.6370934947951596 2315 36-37 0.5490213355115543 2315 38-39 0.6189462068066476 2315 40-41 0.6307777487283559 2315 42-43 0.691761447591908 2315 44-45 0.6761073244175364 2315 46-47 0.4780991456666115 2315 48-49 -4.926076947890799 2315 50-51 -0.9292612687487676 2315 52-53 0.18887216805496365 2315 54-55 0.18725451363305723 2315 56-57 0.5987651372075007 2315 58-59 0.7357536415017734 2315 60-61 0.5313556081789628 2315 62-63 0.2699187769226512 2315 64-65 0.5179092370430993 2315 66-67 -0.24616999024263464 2315 68-69 0.5058870023539725 2315 70-71 0.5456133197531088 2315 72-73 0.5984178786158125 2315 74-75 0.7846996204529191 2315 76-77 0.7175599363152827 2315 78-79 0.6634337481430208 2315 80-81 0.5425025732701343 2315 82-83 0.4549200177737518 2315 84-85 0.4869183572294453 2315 86-87 0.5161140931359753 2315 88-89 0.5577461530415491 2315 90-91 0.5763829531740257 2315 92-93 0.6010981580275896 2315 94-95 0.16314269723995878 2315 96-97 0.39759180689789986 2315 98-99 0.3827685764331754 2315 100 0.3755203298073191 2316 1 0.197021065692482 2316 2 0.25715902102510313 2316 3 0.1327569871165437 2316 4 -0.07918475692250126 2316 5 0.12881498655733736 2316 6 0.1083746906057712 2316 7 0.23406731336054065 2316 8 0.26229264043130485 2316 9 0.4165489876046422 2316 10-11 0.34049962231343045 2316 12-13 0.31356375453356833 2316 14-15 0.43823811581539474 2316 16-17 0.3909190024905982 2316 18-19 0.1466025585977775 2316 20-21 0.4339304205977399 2316 22-23 0.5468608476667214 2316 24-25 0.5673727901968917 2316 26-27 0.5605107838681249 2316 28-29 0.6754253431841732 2316 30-31 0.6038148409789841 2316 32-33 0.5527238922837654 2316 34-35 0.5338505819244261 2316 36-37 0.4263447445838793 2316 38-39 0.4802210194035581 2316 40-41 0.09164640360382492 2316 42-43 0.01603440021879976 2316 44-45 0.2785431778018932 2316 46-47 0.2630295447680808 2316 48-49 -5.323860616651579 2316 50-51 -1.169555187238707 2316 52-53 0.13810116344765078 2316 54-55 0.45034097338041335 2316 56-57 0.3453484584857023 2316 58-59 0.24923749085624536 2316 60-61 0.36930097373933535 2316 62-63 0.34723549324664305 2316 64-65 0.3834109684386746 2316 66-67 0.43431321881843843 2316 68-69 0.10890813350948747 2316 70-71 -0.2956826198240208 2316 72-73 -0.1972931872111161 2316 74-75 0.08430573072337921 2316 76-77 0.18106069560451488 2316 78-79 0.24704789855326936 2316 80-81 0.20104999882159547 2316 82-83 0.14029541709750504 2316 84-85 0.14918591930806357 2316 86-87 0.23892439166693435 2316 88-89 0.052553010149036794 2316 90-91 0.13899511196973435 2316 92-93 0.32549482211621594 2316 94-95 0.5470225804594797 2316 96-97 0.5161667132424022 2316 98-99 0.48744473214800976 2316 100 -0.23656021600656985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 7.0 11 14.0 12 42.0 13 150.0 14 534.0 15 1690.0 16 4081.0 17 8268.0 18 14737.0 19 22770.0 20 33232.0 21 46895.0 22 59444.0 23 62782.0 24 64091.0 25 69739.0 26 82808.0 27 100968.0 28 125287.0 29 153921.0 30 190718.0 31 235799.0 32 295515.0 33 386202.0 34 532749.0 35 750685.0 36 1166666.0 37 1724618.0 38 1409551.0 39 190421.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.636951855992834 20.46881092689387 14.699070047457907 23.19516716965539 2 15.369061185796262 20.50417067158195 40.379062160681215 23.747705981940577 3 18.52780268095849 25.89066401806579 30.165178983548557 25.41635431742716 4 12.750888134783109 17.244499034564416 38.38317375939491 31.621439071257562 5 13.294545454545453 37.84953535353535 34.74270383838384 14.113215353535352 6 30.69756292635019 37.52579870782461 17.687799659676205 14.088838706148984 7 27.36483407053759 32.59606012152719 22.10692204864992 17.9321837592853 8 25.992293867418315 35.611111829430705 19.83337427755007 18.56322002560091 9 26.161873676713494 16.163652928925856 20.829418590547775 36.84505480381288 10-11 25.037401531280945 26.539377984510317 27.655075585937926 20.76814489827081 12-13 25.541194245267523 24.091443686093537 28.08064155465463 22.286720513984314 14-15 22.984955253120024 25.557354590417813 25.817129305799526 25.640560850662634 16-17 22.037268794477953 28.455708696476556 26.779110075992524 22.727912433052968 18-19 21.73492533747276 27.542898597719645 29.073592244106266 21.64858382070133 20-21 22.836678616530893 26.674088059218576 28.915219317341784 21.574014006908747 22-23 22.84597085210476 26.44046548765005 28.64691801227596 22.066645647969228 24-25 22.449098989520778 26.65942585458638 28.92340252142393 21.968072634468907 26-27 22.30624512647977 26.87205560509614 28.655510273002466 22.166188995421624 28-29 22.01656911565508 26.842171645260265 28.983831112962022 22.157428126122632 30-31 22.481437523390372 26.644754088599903 28.847885536205116 22.025922851804612 32-33 22.43996399630653 26.945882352332685 28.455247075411084 22.158906575949704 34-35 22.59088103202339 26.882070698857763 28.731377277590482 21.795670991528368 36-37 22.568752241694263 26.69278428401215 28.53391724235412 22.20454623193947 38-39 22.47457837975126 26.78824248123704 28.7506782805891 21.986500858422602 40-41 22.390043553956286 26.489901520347914 28.976840828617732 22.143214097078065 42-43 22.286661009425732 26.520030085177982 28.676612911671917 22.51669599372437 44-45 22.315017707812146 26.90702362578178 28.472755105926524 22.30520356047955 46-47 22.356871728129505 26.817373943481215 28.141677770902955 22.684076557486325 48-49 22.69396160690468 26.810514547198217 28.067924504104834 22.42759934179227 50-51 22.532241760410272 26.89338671969612 27.892738722738642 22.68163279715497 52-53 22.349257562581023 27.125905618471545 27.796352632718975 22.728484186228457 54-55 22.35894263636718 27.06876062596893 27.779383662930574 22.792913074733313 56-57 22.56873813332671 26.9554958698417 27.597421153051442 22.878344843780145 58-59 22.221531888174678 26.948763450092166 28.004017701055734 22.825686960677423 60-61 22.422489129833263 27.246957982480502 27.663035692212528 22.667517195473707 62-63 22.431648373018707 27.039726231446455 27.641717585797053 22.886907809737785 64-65 22.10062979943716 26.91689756209089 27.765300846845015 23.217171791626935 66-67 22.297973834417423 26.863461638443788 27.62748019888926 23.21108432824953 68-69 22.10105067147878 26.93951702052359 27.71756705977205 23.241865248225572 70-71 22.186282370196384 26.65358399149238 27.787448171571345 23.372685466739892 72-73 22.337904102117612 26.87974792608358 27.59933929528063 23.18300867651818 74-75 22.315035722371878 27.087841488949167 27.470357213374403 23.126765575304557 76-77 22.1572519717693 26.994343799392833 27.44689415866457 23.401510070173302 78-79 22.090696998131953 26.879906011972714 27.519110713546375 23.510286276348953 80-81 22.000032582517488 27.182385425581334 27.449730153400303 23.367851838500872 82-83 22.332747835157836 26.85635971342378 27.727061686016206 23.083830765402173 84-85 22.36393769896004 26.923778005228932 27.340018362632307 23.372265933178717 86-87 21.904138385447077 27.13376669311312 27.617788571897826 23.34430634954197 88-89 22.098785830387445 26.69125766191757 27.63743759156551 23.572518916129468 90-91 22.16800591660652 26.685709763241338 27.641216111450284 23.505068208701854 92-93 22.19361254951494 26.799259083829703 27.522802555693982 23.484325810961376 94-95 21.725874554647024 26.63072616658091 27.744628239320857 23.898771039451216 96-97 21.877780092916883 26.603355405882496 27.665946071539004 23.852918429661617 98-99 21.641921853646167 26.751527947240778 27.596297999158043 24.010252199955012 100 21.754602931369774 26.579371045234936 27.486053721947712 24.179972301447577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1635.0 1 1141.0 2 866.0 3 1141.0 4 942.5 5 808.5 6 1324.5 7 2673.0 8 4500.0 9 5579.0 10 5150.0 11 4435.0 12 4562.5 13 5164.0 14 5908.0 15 6669.0 16 7486.0 17 8275.0 18 9158.5 19 10104.5 20 11147.5 21 12382.5 22 14100.5 23 16514.5 24 19216.5 25 23042.0 26 28636.5 27 34905.5 28 42064.5 29 53073.5 30 68822.0 31 85068.5 32 105506.5 33 130947.0 34 155922.5 35 179649.0 36 206724.5 37 235829.5 38 250755.0 39 254318.5 40 251125.5 41 246140.0 42 247907.0 43 255419.5 44 266393.5 45 284680.5 46 300456.0 47 308681.5 48 316532.5 49 317628.0 50 309844.5 51 300878.0 52 291443.0 53 280386.0 54 269831.0 55 248891.5 56 223147.0 57 194224.5 58 165757.5 59 144451.0 60 119643.5 61 93714.0 62 73622.5 63 57275.0 64 41670.5 65 29178.0 66 21078.5 67 15453.0 68 11552.0 69 8604.5 70 6521.0 71 4969.0 72 3733.5 73 2820.0 74 2025.5 75 1336.5 76 884.0 77 497.5 78 264.5 79 154.0 80 96.5 81 58.5 82 31.0 83 18.0 84 14.0 85 11.0 86 9.5 87 6.0 88 3.5 89 4.0 90 2.0 91 1.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02959504054869645 2 0.0020428206232826727 3 7.757546670693695E-5 4 0.02101002223312876 5 3.749480890835286E-4 6 6.46462222557808E-5 7 3.8787733353468473E-5 8 0.003930490313151473 9 0.010576121961045737 10-11 0.0012282782228598352 12-13 5.818160003020272E-5 14-15 1.292924445115616E-4 16-17 3.167664890533259E-4 18-19 2.5858488902312317E-5 20-21 8.404008893251503E-5 22-23 2.6504951124870125E-4 24-25 5.818160003020272E-5 26-27 6.46462222557808E-5 28-29 9.632287116111337E-4 30-31 0.0023983748456894672 32-33 0.009735721071720587 34-35 0.01911588792103438 36-37 0.021882746233581798 38-39 0.020893659033068356 40-41 0.004124428979918815 42-43 0.004693315735769686 44-45 0.008669058404500204 46-47 0.011235513428054702 48-49 1.199103382755801 50-51 0.01736397529790272 52-53 0.01847589032070215 54-55 0.009018148004681422 56-57 0.007240376892647449 58-59 0.008119565515326067 60-61 0.005068263824853214 62-63 0.007395527826061323 64-65 0.01895427236539493 66-67 0.02924595094851523 68-69 0.029136052370680406 70-71 0.026382123302584142 72-73 0.02264557165620001 74-75 0.009502994671599777 76-77 0.014493683029746053 78-79 0.008442796626604972 80-81 0.002521202667975451 82-83 0.014267421251850822 84-85 0.023951425345766785 86-87 0.02325971076762993 88-89 0.03331219832840384 90-91 0.02059628641069176 92-93 0.013000355295637516 94-95 0.006600379292315219 96-97 0.003717157779707396 98-99 0.01596115227495228 100 0.03351260161739676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7734404.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.83238303304064 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0585174308647 40.231987459930735 2 15.79335023224562 17.635607590833978 3 5.631083150149325 9.431903739901148 4 2.501096289735305 5.585686642040734 5 1.3026050971346848 3.636377336200742 6 0.7220606675919355 2.418862065965159 7 0.4479215144072502 1.7505967892787662 8 0.3209409999498064 1.4335120672163735 9 0.20811170313512323 1.0457435090788783 >10 0.9302192906117099 8.95038813575936 >50 0.04968001803971081 1.9284765856496033 >100 0.03131854484147139 3.3785264423894805 >500 0.00225682043010227 0.87466190744132 >1k 7.0928073053979E-4 0.5620798293086396 >5k 3.2240033199896625E-5 0.10015410778867496 >10k+ 9.672009959968986E-5 1.035435791216382 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24525 0.3170897201646048 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21427 0.277034920854923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11571 0.14960428754432792 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 2 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 3 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 4 0.0 0.0 0.0 1.2929244451156158E-5 2.5858488902312317E-5 5 0.0 0.0 0.0 2.5858488902312317E-5 2.5858488902312317E-5 6 0.0 0.0 0.0 2.5858488902312317E-5 3.8787733353468473E-5 7 0.0 0.0 0.0 2.5858488902312317E-5 5.1716977804624633E-5 8 0.0 0.0 0.0 2.5858488902312317E-5 5.1716977804624633E-5 9 0.0 0.0 0.0 5.1716977804624633E-5 5.1716977804624633E-5 10-11 0.0 0.0 0.0 5.1716977804624633E-5 5.1716977804624633E-5 12-13 0.0 0.0 0.0 5.1716977804624633E-5 5.1716977804624633E-5 14-15 0.0 0.0 0.0 5.1716977804624633E-5 5.1716977804624633E-5 16-17 0.0 0.0 0.0 5.818160003020272E-5 6.46462222557808E-5 18-19 0.0 0.0 0.0 6.46462222557808E-5 7.757546670693695E-5 20-21 0.0 0.0 0.0 9.69693333836712E-5 7.757546670693695E-5 22-23 0.0 0.0 0.0 3.361603557300601E-4 8.404008893251503E-5 24-25 0.0 0.0 0.0 8.016131559716818E-4 9.050471115809311E-5 26-27 0.0 0.0 0.0 0.0017713064898083938 9.050471115809311E-5 28-29 0.0 0.0 0.0 0.004854931291409138 9.050471115809311E-5 30-31 0.0 0.0 0.0 0.014823378763250536 1.0343395560924927E-4 32-33 0.0 0.0 0.0 0.039867325265140016 1.292924445115616E-4 34-35 0.0 0.0 0.0 0.07969586279692656 1.551509334138739E-4 36-37 0.0 0.0 0.0 0.1425837078073501 1.6808017786503005E-4 38-39 0.0 1.2929244451156158E-5 0.0 0.22963631069698454 1.6808017786503005E-4 40-41 0.0 1.2929244451156158E-5 0.0 0.32129172461123057 2.0686791121849853E-4 42-43 0.0 1.2929244451156158E-5 0.0 0.41312814794779273 2.2626177789523277E-4 44-45 0.0 1.2929244451156158E-5 0.0 0.5270218623180274 2.585848890231232E-4 46-47 0.0 1.2929244451156158E-5 0.0 0.6651514454119541 3.2969573350448205E-4 48-49 0.0 1.2929244451156158E-5 0.0 0.8125125090440064 3.749480890835286E-4 50-51 0.0 1.2929244451156158E-5 0.0 0.9690791947252819 3.749480890835286E-4 52-53 0.0 2.5858488902312317E-5 0.0 1.1210042816485926 4.2666506688815323E-4 54-55 0.0 2.5858488902312317E-5 0.0 1.2910367754257472 4.91311289143934E-4 56-57 0.0 2.5858488902312317E-5 0.0 1.493806116153229 5.042405335950902E-4 58-59 0.0 2.5858488902312317E-5 0.0 1.7188137573367 5.042405335950902E-4 60-61 0.0 2.5858488902312317E-5 0.0 1.9502730915012974 5.042405335950902E-4 62-63 0.0 2.5858488902312317E-5 0.0 2.180853237043216 5.171697780462464E-4 64-65 0.0 2.5858488902312317E-5 0.0 2.41914567690025 5.171697780462464E-4 66-67 0.0 2.5858488902312317E-5 0.0 2.6896901170406924 5.300990224974025E-4 68-69 0.0 2.5858488902312317E-5 0.0 2.9878837464399326 5.300990224974025E-4 70-71 0.0 2.5858488902312317E-5 0.0 3.307203761272362 6.270683558810737E-4 72-73 0.0 2.5858488902312317E-5 0.0 3.627073268993965 7.111084448135887E-4 74-75 0.0 2.5858488902312317E-5 0.0 3.946141163559597 7.111084448135887E-4 76-77 0.0 2.5858488902312317E-5 0.0 4.2925026931616195 7.111084448135887E-4 78-79 0.0 2.5858488902312317E-5 0.0 4.674736411493374 7.111084448135887E-4 80-81 0.0 2.5858488902312317E-5 0.0 5.079046814725478 7.434315559414792E-4 82-83 0.0 2.5858488902312317E-5 0.0 5.488748712893715 8.016131559716818E-4 84-85 0.0 5.1716977804624633E-5 0.0 5.887764590523071 8.080777781972599E-4 86-87 0.0 5.1716977804624633E-5 0.0 6.305657165051114 8.14542400422838E-4 88 0.0 5.1716977804624633E-5 0.0 6.63841712949052 8.404008893251504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14155 0.0 50.762 1 GTATCAA 25560 0.0 41.02001 1 CAACGCA 31400 0.0 32.450687 5 ATCAACG 31440 0.0 32.40349 3 TATCAAC 32940 0.0 31.451355 2 TCAACGC 33275 0.0 30.68726 4 AACGCAG 33900 0.0 30.334341 6 GTGGTAT 6740 0.0 27.8531 1 ACGCAGA 37895 0.0 26.752634 7 CGCAGAG 38610 0.0 26.208614 8 GTACTAG 3140 0.0 25.259834 1 TGGTATC 7055 0.0 25.206127 2 GCAGAGT 42045 0.0 23.865496 9 GTACATG 33595 0.0 23.679281 1 TACATGG 34485 0.0 22.844643 2 TACCTGG 4745 0.0 22.34787 2 GAGTACT 22535 0.0 22.11483 12-13 ACATGGG 34715 0.0 21.682116 3 GTACTTT 23890 0.0 20.7329 14-15 CAGAGTA 41970 0.0 20.093971 10-11 >>END_MODULE