FastQCFastQC Report
Mon 6 Jun 2016
ERR1211312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263094
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281100.30346232047305144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222920.24065393269246754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126820.1369088989057004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151000.051.4322281
GTATCAA278450.041.1017271
ATCAACG335500.033.2657553
CAACGCA336500.033.1941075
TATCAAC353250.031.9976462
TCAACGC357400.031.3846384
AACGCAG360850.031.2797456
GTACTAG38100.027.6559281
ACGCAGA404050.027.5523457
CGCAGAG412950.026.8664768
GTACATG398150.026.5473731
TACATGG406550.025.4886572
ACATGGG407000.024.5237223
GCAGAGT449350.023.8126059
GTGGTAT74050.023.1228581
GCATCGT40100.022.147758
GAGTACT233600.021.80668812-13
TGGTATC75000.021.4492072
CATGGGG275850.021.2598824
TAGTACT51150.020.5788734
Produced by FastQC (version 0.11.5)