FastQCFastQC Report
Mon 6 Jun 2016
ERR1211312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263094
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257500.2779848720092876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215780.23294592497927796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121770.13145715675561534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156650.050.9851881
GTATCAA279100.039.259241
CAACGCA346250.030.6731555
ATCAACG348750.030.4985713
TATCAAC363550.029.7707962
TCAACGC366500.029.1242184
AACGCAG371350.028.9409986
GTACTAG38350.027.8987241
ACGCAGA416750.025.5180647
CGCAGAG426450.024.8936318
GTACATG395400.024.6498721
TACATGG403700.023.8814142
ACATGGG406550.022.9196223
GTGGTAT79150.022.6478671
GCAGAGT469300.022.4200449
TGGTATC80250.021.7472782
GAGTACT242450.020.9415312-13
CATGGGG271550.020.4918774
TACCTGG60700.019.7860132
GTACTTT256500.019.69381314-15
Produced by FastQC (version 0.11.5)