FastQCFastQC Report
Mon 6 Jun 2016
ERR1211311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228656
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298870.29218892491838616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234350.22911123416409743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132550.12958691738191214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161500.053.8820231
GTATCAA296850.042.1175041
CAACGCA360800.033.8518755
ATCAACG368800.033.206923
TATCAAC382500.032.2182352
TCAACGC384600.031.9398524
AACGCAG388650.031.6192636
ACGCAGA437900.027.7094927
CGCAGAG449950.026.819358
GTACATG432300.026.2661671
GTGGTAT74700.025.754161
GTACTAG39300.025.2542571
TACATGG446350.025.1435622
GCAGAGT484000.024.283389
ACATGGG452900.024.0937843
TGGTATC82250.021.9025552
GAGTACT252750.021.71546712-13
CATGGGG311250.021.7113134
CAGAGTA482550.020.56279810-11
GTACTTT272200.020.08664714-15
Produced by FastQC (version 0.11.5)