FastQCFastQC Report
Mon 6 Jun 2016
ERR1211311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228656
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270080.26404250959265807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228160.2230596082222337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127960.12509952431678217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170750.051.3401031
GTATCAA301300.040.2158931
CAACGCA371550.031.9441225
ATCAACG380850.031.144773
TCAACGC391250.030.448964
TATCAAC396050.030.4329622
AACGCAG400150.030.0478536
ACGCAGA451800.026.2078157
CGCAGAG466650.025.3336038
GTGGTAT78100.024.3958281
GTACTAG43350.024.0328031
GTACATG442900.023.5439321
GCAGAGT513050.022.7124889
TACATGG455350.022.6470952
ACATGGG455600.021.9875183
GAGTACT256600.021.69797112-13
TGGTATC86200.020.7915212
GTACTTT273550.020.09624514-15
GGGCTAT69100.019.6882746
CATGGGG313400.019.5677434
Produced by FastQC (version 0.11.5)