FastQCFastQC Report
Mon 6 Jun 2016
ERR1211310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10575064
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299630.28333634671147145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235540.2227315125468744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131670.12450988476287235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.049.1517941
GTATCAA296000.041.750131
CAACGCA360250.033.3407635
ATCAACG361650.033.2898183
TATCAAC381400.032.1039352
AACGCAG390800.031.3718596
TCAACGC390600.030.9429364
ACGCAGA438050.027.4632177
CGCAGAG450100.026.6543878
GTACTAG45750.026.5288281
GTACATG442200.025.7765121
TACATGG454450.024.7593842
GCAGAGT492950.023.7770239
ACATGGG466950.023.3876443
GTGGTAT79650.023.0339431
GCATCGT46000.021.8616458
GAGTACT250550.021.5886412-13
CATGGGG309200.021.322644
TGGTATC85000.020.697482
TAGTACT66050.020.2054394
Produced by FastQC (version 0.11.5)