FastQCFastQC Report
Mon 6 Jun 2016
ERR1211310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10575064
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266820.2523105297518767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219110.20719496354821115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126160.11929951440483007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170800.049.0710331
GTATCAA304600.040.395631
CAACGCA367250.032.508385
ATCAACG370650.032.216883
TATCAAC392450.031.0095922
TCAACGC394000.030.4147574
AACGCAG399150.030.2508536
ACGCAGA447900.026.6545857
GTACTAG45900.026.1733251
CGCAGAG459750.025.8756168
GTACATG436950.024.4225121
TACATGG449000.023.3320452
GCAGAGT510500.022.9801249
GTGGTAT80550.022.8959471
ACATGGG447550.022.4143333
GAGTACT259050.021.17354412-13
TGGTATC87950.020.4302332
TAGTACT68150.020.241434
CTGTGCG67300.020.0729799
CTAGTAC69900.020.0702933
Produced by FastQC (version 0.11.5)