##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10575064 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12746702998677 33.0 31.0 34.0 30.0 34.0 2 32.268549864095384 34.0 31.0 34.0 30.0 34.0 3 32.336564487931234 34.0 31.0 34.0 30.0 34.0 4 35.96586602218199 37.0 35.0 37.0 35.0 37.0 5 35.87250450682852 37.0 35.0 37.0 35.0 37.0 6 35.883644108442276 37.0 35.0 37.0 35.0 37.0 7 35.79670165589542 37.0 35.0 37.0 33.0 37.0 8 35.7778282003778 37.0 35.0 37.0 33.0 37.0 9 37.39748383555882 39.0 37.0 39.0 34.0 39.0 10-11 37.34875316121018 39.0 37.0 39.0 34.0 39.0 12-13 37.28292443431076 39.0 37.0 39.0 34.0 39.0 14-15 38.53416073888536 40.0 38.0 41.0 34.0 41.0 16-17 38.51878116293197 40.0 38.0 41.0 34.0 41.0 18-19 38.51923647932533 40.0 38.0 41.0 34.0 41.0 20-21 38.48459824923991 40.0 38.0 41.0 34.0 41.0 22-23 38.381857641712614 40.0 38.0 41.0 34.0 41.0 24-25 38.29638198879931 40.0 38.0 41.0 34.0 41.0 26-27 38.202043458082144 40.0 38.0 41.0 33.5 41.0 28-29 38.10370764659202 40.0 37.5 41.0 33.0 41.0 30-31 38.11251066660211 40.0 37.5 41.0 33.0 41.0 32-33 38.059087822068975 40.0 37.0 41.0 33.0 41.0 34-35 37.94036627106938 40.0 37.0 41.0 33.0 41.0 36-37 37.803282656256265 40.0 37.0 41.0 33.0 41.0 38-39 37.693380248100624 40.0 37.0 41.0 33.0 41.0 40-41 37.58871690989294 40.0 37.0 41.0 32.5 41.0 42-43 37.48677790507935 40.0 36.5 41.0 32.0 41.0 44-45 37.329875119431904 40.0 36.0 41.0 31.5 41.0 46-47 37.10054681465758 39.5 36.0 41.0 31.0 41.0 48-49 35.52967182988207 39.0 35.0 41.0 24.5 41.0 50-51 36.28271398641181 39.0 35.0 40.0 29.0 41.0 52-53 36.38202435465166 39.0 35.0 40.0 30.0 41.0 54-55 36.21657136070287 38.5 35.0 40.0 29.0 41.0 56-57 36.00064363676664 38.0 35.0 40.0 29.0 41.0 58-59 35.76211865006207 38.0 34.5 40.0 28.0 41.0 60-61 35.511316574537986 37.5 34.0 40.0 28.0 41.0 62-63 35.193182045990454 37.0 34.0 40.0 28.0 41.0 64-65 34.86085635037291 36.5 34.0 39.5 27.0 41.0 66-67 34.525261360120375 36.0 33.5 39.0 26.5 40.5 68-69 34.17089173171907 35.5 33.0 39.0 26.0 40.0 70-71 33.81926544368904 35.0 33.0 38.0 26.0 40.0 72-73 33.44648216786206 35.0 32.0 37.5 26.0 39.5 74-75 33.08685394244422 35.0 32.0 37.0 26.0 39.0 76-77 32.14820283829961 34.0 30.5 36.0 24.5 38.5 78-79 32.57410049716957 35.0 32.0 36.0 25.5 38.0 80-81 32.4493212050537 35.0 32.0 36.0 25.0 37.0 82-83 32.180951907241415 34.5 31.5 35.0 25.0 37.0 84-85 31.905080243485997 34.0 31.0 35.0 25.0 36.5 86-87 31.64860841504127 34.0 31.0 35.0 24.0 36.0 88-89 31.42369058002864 34.0 31.0 35.0 24.0 36.0 90-91 31.207238462102925 34.0 31.0 35.0 23.0 35.0 92-93 31.024567463610623 34.0 30.5 35.0 23.0 35.0 94-95 30.83543054680331 34.0 30.0 35.0 21.5 35.0 96-97 30.652913873618164 34.0 30.0 35.0 20.0 35.0 98-99 30.441103855257992 34.0 29.5 35.0 20.0 35.0 100 28.025767692753444 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2737293931574669 1101 2 -0.25291320947448526 1101 3 -0.3342299349921305 1101 4 -0.18054231643287721 1101 5 -0.18702051647661477 1101 6 -0.24870512130913625 1101 7 -0.24383878223894584 1101 8 -0.22371517625782644 1101 9 -0.3622514843421527 1101 10-11 -0.33996527630274187 1101 12-13 -0.3977706918231121 1101 14-15 -0.454934906439334 1101 16-17 -0.5078402880490742 1101 18-19 -0.55642675926876 1101 20-21 -0.5762655287579506 1101 22-23 -0.6799548922324874 1101 24-25 -0.6052790502637819 1101 26-27 -0.644963867341076 1101 28-29 -0.7175318770857686 1101 30-31 -0.6256465529810242 1101 32-33 -0.5773892751668654 1101 34-35 -0.627983590380012 1101 36-37 -0.752061100738608 1101 38-39 -0.7202512078849992 1101 40-41 -0.6560688640851353 1101 42-43 -0.6542898241269697 1101 44-45 -0.6625752380793344 1101 46-47 -0.6504312990523076 1101 48-49 0.5611804691352589 1101 50-51 -0.3806044907539601 1101 52-53 -0.706955102058977 1101 54-55 -0.7558870667436253 1101 56-57 -0.7438427259817217 1101 58-59 -0.9222869035135943 1101 60-61 -0.918972473551591 1101 62-63 -0.9501253274113779 1101 64-65 -0.9163742465351632 1101 66-67 -0.8099968908222479 1101 68-69 -0.8824723629379321 1101 70-71 -0.8609932904714981 1101 72-73 -0.958461316929899 1101 74-75 -0.9489029790252133 1101 76-77 -0.8982496844112475 1101 78-79 -0.8625767537476676 1101 80-81 -0.7754484323362583 1101 82-83 -0.8086217527071895 1101 84-85 -0.7765803613049336 1101 86-87 -0.7315756153625941 1101 88-89 -0.7428888730888268 1101 90-91 -0.8794012963882807 1101 92-93 -0.8728188832687032 1101 94-95 -0.9685085423992952 1101 96-97 -0.9791620237898435 1101 98-99 -1.0932553626598143 1101 100 -0.9409657223357613 1102 1 -0.2241164635588042 1102 2 -0.21850667180866168 1102 3 -0.23578298970308964 1102 4 -0.11151456082377109 1102 5 -0.14870320698428685 1102 6 -0.21479330071309732 1102 7 -0.23676002577746402 1102 8 -0.233254271605432 1102 9 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0.43905624947028343 2311 10-11 0.36779387307407063 2311 12-13 0.46641079861471013 2311 14-15 0.4407914250884062 2311 16-17 0.47785388405193174 2311 18-19 0.6348415444464734 2311 20-21 0.6528477869447542 2311 22-23 0.7052107563767507 2311 24-25 0.6459070068202806 2311 26-27 0.7099981158034012 2311 28-29 0.7601922280410918 2311 30-31 0.623626270926664 2311 32-33 0.6536479466311675 2311 34-35 0.789458341852864 2311 36-37 0.8194011660356253 2311 38-39 0.8158595161113027 2311 40-41 0.7657185409654161 2311 42-43 0.8300399727217211 2311 44-45 0.8222243852961171 2311 46-47 0.8467502597576342 2311 48-49 -5.920827497578532 2311 50-51 -1.0124161030206338 2311 52-53 0.4148858093466856 2311 54-55 0.8436094277409154 2311 56-57 0.9602556121943664 2311 58-59 0.9743158886637673 2311 60-61 0.8715410953133045 2311 62-63 0.7743152807797955 2311 64-65 0.832938024917155 2311 66-67 0.9225238258177768 2311 68-69 0.9843918604126998 2311 70-71 0.9629183089713251 2311 72-73 0.3902007951946871 2311 74-75 0.7465740123969766 2311 76-77 0.8558650052764278 2311 78-79 0.7788328250648746 2311 80-81 0.7629101065332335 2311 82-83 0.8048991344814453 2311 84-85 0.8348362161072309 2311 86-87 0.866234994113146 2311 88-89 0.8989013186431443 2311 90-91 0.9433401122254672 2311 92-93 0.9437698397387635 2311 94-95 0.9766316455051403 2311 96-97 1.0290308250327698 2311 98-99 1.0537162517921494 2311 100 1.2809683447055455 2312 1 0.09317224834278193 2312 2 0.23532130273047613 2312 3 0.212281509371266 2312 4 0.009248337524098815 2312 5 0.16274968359670794 2312 6 0.11380247304071389 2312 7 0.29057533754399856 2312 8 0.3246425708799592 2312 9 0.2650699219477701 2312 10-11 0.2160990901635813 2312 12-13 0.3067698568320196 2312 14-15 0.30768797516783764 2312 16-17 0.41577997665931576 2312 18-19 0.3307257066366418 2312 20-21 0.4048513989045759 2312 22-23 0.6391632135362357 2312 24-25 0.6981961917986226 2312 26-27 0.6127891128174952 2312 28-29 0.7283368249783564 2312 30-31 0.7719390819315279 2312 32-33 0.7639181758677367 2312 34-35 0.38997209139492384 2312 36-37 0.6598728333893362 2312 38-39 0.8070515121566757 2312 40-41 0.6069363627539559 2312 42-43 0.6061826694116306 2312 44-45 0.7482349575054101 2312 46-47 0.7716915341934794 2312 48-49 -4.937148091783918 2312 50-51 -0.868817280618785 2312 52-53 0.4797417184203496 2312 54-55 0.7253694057812581 2312 56-57 0.819176836228479 2312 58-59 0.8529475567591831 2312 60-61 0.889021798086489 2312 62-63 0.6344486239310996 2312 64-65 0.8328238214336707 2312 66-67 0.8701451162410407 2312 68-69 0.7877984817281032 2312 70-71 0.8020891900734384 2312 72-73 0.9039094335440367 2312 74-75 0.5813960505716977 2312 76-77 0.8128673267529649 2312 78-79 0.4601866170599749 2312 80-81 0.6730877135827811 2312 82-83 0.7177770295745844 2312 84-85 0.821136406957276 2312 86-87 0.8681195659567358 2312 88-89 0.9030457769928013 2312 90-91 0.6913255809978089 2312 92-93 0.8707369934020939 2312 94-95 0.9964958703716391 2312 96-97 1.0664042767475799 2312 98-99 1.108437499363653 2312 100 0.6446291606811272 2313 1 0.04120170805893508 2313 2 0.21838098232272074 2313 3 0.2968315790800631 2313 4 0.04400425576083933 2313 5 0.1368557187141235 2313 6 0.25659037387283234 2313 7 0.15858956633167764 2313 8 0.25690138019920994 2313 9 0.45830248416706354 2313 10-11 0.21354487115036136 2313 12-13 0.05686783945470353 2313 14-15 0.15425204073486043 2313 16-17 0.08889525193961134 2313 18-19 0.4190613770253506 2313 20-21 0.41359049138902293 2313 22-23 0.4991271450484902 2313 24-25 0.30946596313797414 2313 26-27 0.4543833038226879 2313 28-29 0.4344554493258812 2313 30-31 0.6127856633019917 2313 32-33 0.4585829900162466 2313 34-35 0.40336890968949035 2313 36-37 -0.2734114384364048 2313 38-39 0.1522915827931044 2313 40-41 0.3819962458193942 2313 42-43 0.45137445505919516 2313 44-45 0.4414068021523505 2313 46-47 0.36727905662510096 2313 48-49 -5.11976773378516 2313 50-51 -1.2752331306938416 2313 52-53 0.09096118390375096 2313 54-55 0.28129488101725286 2313 56-57 0.2844440173525271 2313 58-59 0.3540826889092372 2313 60-61 0.6668104860757609 2313 62-63 0.7865356851864718 2313 64-65 0.8354068623276945 2313 66-67 0.8857306578305355 2313 68-69 0.8132801504887084 2313 70-71 0.8195255254359779 2313 72-73 0.6258790132293228 2313 74-75 0.7409642309118425 2313 76-77 0.7923198305693333 2313 78-79 0.7328802021294294 2313 80-81 -0.15505174722529347 2313 82-83 0.20650817776245134 2313 84-85 0.5960602967870834 2313 86-87 0.7116237575620268 2313 88-89 0.6546770091179361 2313 90-91 0.656027115543548 2313 92-93 0.7768878613703869 2313 94-95 0.6955703799627919 2313 96-97 0.7196375227916647 2313 98-99 0.9121142727549483 2313 100 0.8611040224289681 2314 1 0.11792421918628548 2314 2 0.2277717579754608 2314 3 0.25913805869944184 2314 4 0.0774843232735094 2314 5 0.21987606311400043 2314 6 0.3034433421388627 2314 7 0.13720886894390816 2314 8 0.25737065631077627 2314 9 0.44460846011624255 2314 10-11 0.3179109302147367 2314 12-13 0.27667289460568867 2314 14-15 0.3004220268697608 2314 16-17 0.5184221634897241 2314 18-19 0.5931267398670812 2314 20-21 0.6701542807044447 2314 22-23 0.35463102873840313 2314 24-25 0.49315559089787087 2314 26-27 0.5356087534924612 2314 28-29 0.6558405659170887 2314 30-31 0.5469520738696403 2314 32-33 0.5205763726800043 2314 34-35 0.4771759997754259 2314 36-37 0.5615286042646659 2314 38-39 0.5609519645026566 2314 40-41 0.34676413377320614 2314 42-43 0.37709715527172705 2314 44-45 0.5807692148991848 2314 46-47 0.6559347569665022 2314 48-49 -6.3356329159015985 2314 50-51 -1.4408694073132864 2314 52-53 -0.5927310768962855 2314 54-55 -0.24721190216886413 2314 56-57 0.03340803631461853 2314 58-59 0.5010766425502879 2314 60-61 0.689693355019088 2314 62-63 0.5631340047979663 2314 64-65 0.7401656735085496 2314 66-67 0.825172028868721 2314 68-69 0.8397437894638671 2314 70-71 0.5624681835054446 2314 72-73 0.7936450015839185 2314 74-75 0.8369935025710333 2314 76-77 0.7486347640451285 2314 78-79 0.563189271546328 2314 80-81 0.6052612982088164 2314 82-83 0.6365876131657373 2314 84-85 0.6799049631408387 2314 86-87 0.705387199437677 2314 88-89 0.6089062045278304 2314 90-91 0.8331035465087737 2314 92-93 0.9053799451035438 2314 94-95 0.9538658488918621 2314 96-97 0.9213967018512683 2314 98-99 0.9324623247109578 2314 100 -0.4881081168937449 2315 1 0.1153447191620316 2315 2 0.1705526512322919 2315 3 0.2644636338518467 2315 4 0.0951384946762559 2315 5 0.22023026162898418 2315 6 0.25356191511084347 2315 7 0.3026305192995764 2315 8 0.23941963750770157 2315 9 0.42160210496646044 2315 10-11 0.18437902098735748 2315 12-13 0.37006609542562074 2315 14-15 0.2439021278506317 2315 16-17 0.3284161218997568 2315 18-19 0.4018862829076326 2315 20-21 0.33993012210247997 2315 22-23 0.3905316242041579 2315 24-25 0.4153744149562755 2315 26-27 0.47776590051995527 2315 28-29 0.5534716000864961 2315 30-31 0.5433719682474063 2315 32-33 0.6057617372006447 2315 34-35 0.6289485455702675 2315 36-37 0.5305096760995056 2315 38-39 0.6177410104662613 2315 40-41 0.6412299134101431 2315 42-43 0.6544903910312172 2315 44-45 0.6067288021746364 2315 46-47 0.5472611635996216 2315 48-49 -4.859976286662459 2315 50-51 -0.930837026748982 2315 52-53 0.16445585339120328 2315 54-55 0.14213219256540555 2315 56-57 0.5142923360989116 2315 58-59 0.633149452829251 2315 60-61 0.4754241408209978 2315 62-63 0.19065260515862548 2315 64-65 0.4469772515745518 2315 66-67 -0.17422728021413292 2315 68-69 0.46875718253643583 2315 70-71 0.46761058221445495 2315 72-73 0.5783701074733116 2315 74-75 0.6939348896813584 2315 76-77 0.6531290863226431 2315 78-79 0.5599252250326856 2315 80-81 0.4339304097694239 2315 82-83 0.4366448456272636 2315 84-85 0.4540087397903143 2315 86-87 0.4766539798455689 2315 88-89 0.5445497998473066 2315 90-91 0.6056553623604337 2315 92-93 0.6095365237825661 2315 94-95 0.3260591139308957 2315 96-97 0.3907567852204892 2315 98-99 0.43931715247250125 2315 100 0.38714795138371016 2316 1 0.1911202001801513 2316 2 0.21561547581045204 2316 3 0.1440921963357269 2316 4 -0.08298241485957192 2316 5 0.09397369680187495 2316 6 0.1026524293114619 2316 7 0.25092231518444663 2316 8 0.2810866789151589 2316 9 0.37325379732787667 2316 10-11 0.3813794327306468 2316 12-13 0.291845184414413 2316 14-15 0.3906979323840858 2316 16-17 0.4207033348984268 2316 18-19 0.15268550396552882 2316 20-21 0.4668146409186207 2316 22-23 0.5405075506871881 2316 24-25 0.5873308405516013 2316 26-27 0.5738875202611524 2316 28-29 0.6241152396303846 2316 30-31 0.5347306329299215 2316 32-33 0.5169276995567245 2316 34-35 0.49079799218506537 2316 36-37 0.32944632467789603 2316 38-39 0.5021315452616335 2316 40-41 0.07630355730236715 2316 42-43 0.02296307379162954 2316 44-45 0.32115731345461285 2316 46-47 0.2833412063130041 2316 48-49 -5.242919751464296 2316 50-51 -1.1784701596894749 2316 52-53 0.12211021114493548 2316 54-55 0.41153740768397284 2316 56-57 0.3154106139943593 2316 58-59 0.2755655964504058 2316 60-61 0.38584447215788487 2316 62-63 0.27981312203413466 2316 64-65 0.34489609110623576 2316 66-67 0.420457078058007 2316 68-69 0.13067617809502963 2316 70-71 -0.28215366582628576 2316 72-73 -0.13100377367278782 2316 74-75 0.09213419111736698 2316 76-77 0.10027091008317512 2316 78-79 0.2306538229459889 2316 80-81 0.1746811354107578 2316 82-83 0.04777517518660801 2316 84-85 0.2501743759955879 2316 86-87 0.2582680226310963 2316 88-89 0.09141604764976918 2316 90-91 0.13452298671635177 2316 92-93 0.23818782754199574 2316 94-95 0.4594733752753619 2316 96-97 0.5245555748296233 2316 98-99 0.48816988858724386 2316 100 -0.18670123143041195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 12.0 11 12.0 12 52.0 13 199.0 14 765.0 15 2171.0 16 5276.0 17 10464.0 18 18169.0 19 27912.0 20 40843.0 21 58592.0 22 74157.0 23 79129.0 24 82105.0 25 92256.0 26 110568.0 27 136527.0 28 169037.0 29 208272.0 30 256384.0 31 317400.0 32 400661.0 33 520806.0 34 724837.0 35 1019237.0 36 1595513.0 37 2377769.0 38 1972621.0 39 273289.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.014838977036256 20.063626155472985 14.260425872605992 23.661108994884767 2 15.34252420177546 20.198189212058352 40.49568958180376 23.963597004362427 3 18.858033686434627 25.4166549252023 29.565899307597178 26.159412080765893 4 12.716035592297434 16.799462617863284 38.20317293431209 32.28132885552719 5 13.504122171822472 37.77518655305036 34.59715586030762 14.123535414819543 6 31.411150385907977 37.11871138319783 17.537006882230454 13.933131348663744 7 27.995191327064937 32.067764350061786 21.78207232697236 18.15497199590092 8 26.110849688234307 35.70988163343738 19.635262129811338 18.544006548516975 9 26.22155017046184 16.009378167285345 20.493826273148816 37.27524538910401 10-11 25.303757056955266 26.366410890577676 27.518791005849252 20.81104104661781 12-13 25.665871241331605 23.706704125888706 28.03648302538379 22.590941607395894 14-15 23.0165800185219 25.26199761637027 25.62136239847978 26.10005996662805 16-17 22.35281134783079 28.19003604293909 26.504153583364882 22.952999025865235 18-19 22.077929937627793 27.470304432228914 28.57530966954996 21.876455960593333 20-21 23.08944201112457 26.569375479104913 28.44511923976591 21.896063270004607 22-23 23.06490602324692 26.395107609804242 28.14601426160697 22.39397210534187 24-25 22.62520755199426 26.561728580099913 28.505731550862922 22.3073323170429 26-27 22.580644246172856 26.78253176318577 28.135153314067246 22.501670676574133 28-29 22.281192793784594 26.834285669976172 28.424032462740144 22.460489073499087 30-31 22.800794531520026 26.603822205368715 28.346354086631546 22.249029176479713 32-33 22.71963459169724 26.830667140342694 27.99335485428953 22.456343413670535 34-35 22.856577809983275 26.797238589399896 28.243544486516196 22.102639114100633 36-37 22.79009322537671 26.59214973566517 28.09353560776202 22.524221431196096 38-39 22.71562964753721 26.675742783087625 28.315246362270596 22.293381207104566 40-41 22.681151207406778 26.33655251419098 28.527049928061004 22.455246350341234 42-43 22.53276045341231 26.419875766529664 28.2291720796361 22.818191700421924 44-45 22.550953441428693 26.81514564445478 28.079670594806295 22.554230319310236 46-47 22.610656530533024 26.68267385904305 27.739002912721393 22.967666697702533 48-49 22.986433864731552 26.708892461796594 27.699646749333716 22.605026924138134 50-51 22.798059774608237 26.742351806610387 27.563302972169517 22.89628544661186 52-53 22.59358831215446 26.930800853302767 27.505626340970213 22.969984493572564 54-55 22.559464052181884 26.871993602808093 27.502979119236127 23.0655632257739 56-57 22.809260417648318 26.77086273361804 27.311937515270024 23.107939333463616 58-59 22.460560272656785 26.840429979725126 27.63822759472537 23.060782152892713 60-61 22.61874089525983 27.05614461120664 27.409726156934717 22.915388336598813 62-63 22.65264579423476 26.843615637443275 27.33412393426097 23.169614634061 64-65 22.324345551372343 26.745625107704136 27.456425106115173 23.47360423480835 66-67 22.519350611557222 26.711664264920802 27.310586484914356 23.458398638607616 68-69 22.331334577111075 26.774508033121435 27.38756209961677 23.50659529015072 70-71 22.346066059186406 26.530157256233966 27.45436461045897 23.669412074120658 72-73 22.552553224302173 26.742519432528226 27.254595831173457 23.450331511996144 74-75 22.54997658649711 26.896367244776627 27.14980901551552 23.403847153210748 76-77 22.375004693356203 26.85255162999197 27.05215995109263 23.7202837255592 78-79 22.345086702988375 26.805289122736635 27.1064064784987 23.743217695776288 80-81 22.215596387968382 27.118346509507678 27.03341794038243 23.63263916214151 82-83 22.526288531604 26.754995836517203 27.406332986222747 23.31238264565605 84-85 22.634158776040366 26.834152959011465 26.933562672456578 23.598125592491588 86-87 22.090002352338722 27.115182402728145 27.183728437013272 23.61108680791986 88-89 22.334352510882514 26.683465083847434 27.15000072128352 23.832181683986537 90-91 22.365826562908847 26.698696456928495 27.142908690411677 23.792568289750978 92-93 22.41914630788343 26.779921804227406 27.02491902111388 23.776012866775282 94-95 21.996256880633304 26.65691853639408 27.261840708572183 24.08498387440043 96-97 22.06057737567387 26.629037394355326 27.17663432598172 24.13375090398909 98-99 21.8536368346297 26.796678797109504 27.077454192114963 24.272230176145833 100 21.923639397545458 26.668177028251645 26.91608128330915 24.49210229089375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1427.0 1 987.5 2 784.0 3 998.5 4 852.0 5 788.0 6 1244.5 7 2377.0 8 3858.0 9 4839.5 10 4666.5 11 4324.5 12 4692.0 13 5394.0 14 6243.5 15 7158.0 16 8103.5 17 9254.5 18 10316.5 19 11331.0 20 12745.0 21 14368.5 22 16337.0 23 19054.5 24 22191.5 25 26452.5 26 32762.5 27 40851.0 28 50622.0 29 64712.5 30 83833.0 31 104977.0 32 132551.0 33 166069.0 34 198642.0 35 228144.0 36 261611.0 37 301563.5 38 325151.5 39 332042.5 40 331706.5 41 327586.5 42 333407.5 43 350419.5 44 369667.0 45 397747.0 46 424368.0 47 439215.5 48 451709.0 49 454762.5 50 445511.5 51 434790.5 52 422892.5 53 405902.0 54 387309.5 55 353876.5 56 314973.0 57 273790.5 58 233450.5 59 203317.0 60 167650.0 61 131673.0 62 103555.0 63 80670.0 64 59768.5 65 41067.5 66 28442.0 67 21173.5 68 16018.5 69 11600.0 70 8645.0 71 6827.0 72 5397.5 73 4092.5 74 3015.5 75 2037.5 76 1343.5 77 827.0 78 477.0 79 268.5 80 173.5 81 111.0 82 69.0 83 47.5 84 33.5 85 24.5 86 16.0 87 9.0 88 8.5 89 6.5 90 2.0 91 1.0 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03130950318598545 2 0.0020803656601983684 3 5.673724527813733E-5 4 0.022004594960370927 5 5.295476225959483E-4 6 3.782483018542488E-5 7 4.72810377317811E-5 8 0.004312030641138436 9 0.01080844522548516 10-11 0.0011772978395213495 12-13 8.510586791720598E-5 14-15 1.4657121696852143E-4 16-17 3.640639905347145E-4 18-19 9.45620754635622E-6 20-21 5.2009141504959215E-5 22-23 2.6950191507115227E-4 24-25 2.8368622639068664E-5 26-27 6.619345282449354E-5 28-29 0.0010354547263260062 30-31 0.0026382819054333855 32-33 0.00973516566897373 34-35 0.018813124913475702 36-37 0.02173982114907295 38-39 0.02137102905476506 40-41 0.004534251518477808 42-43 0.005092167763712825 44-45 0.009215074253924137 46-47 0.01206139272537736 48-49 1.227321177441574 50-51 0.018557807309724086 52-53 0.01918664511155677 54-55 0.009924289819900853 56-57 0.007872292782341554 58-59 0.008425480923803392 60-61 0.005281291914639949 62-63 0.007839196055929306 64-65 0.020028247583182477 66-67 0.03064284055396733 68-69 0.03063338434642098 70-71 0.02764522276177241 72-73 0.023834371120590853 74-75 0.009810815329344578 76-77 0.015196125526994448 78-79 0.00895975665017252 80-81 0.002789581226175085 82-83 0.014983360857201432 84-85 0.025120415346895297 86-87 0.024652333073350668 88-89 0.03434021770459261 90-91 0.021772917875485197 92-93 0.013943178027102246 94-95 0.006888847197520506 96-97 0.0038439483675938033 98-99 0.015754041772229464 100 0.03445842029892207 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0575064E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.41404591052574 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3876824662193 39.5588031362911 2 15.754670789355682 17.46060100853149 3 5.788681539977062 9.623227938531054 4 2.7353073039043863 6.062977780718162 5 1.4061278190648105 3.8959615760862425 6 0.8088707242822207 2.689367967063311 7 0.5133029223449552 1.9910934193381264 8 0.3401178027106846 1.507784282751761 9 0.23207953550978136 1.1574419432069263 >10 0.9553677559124568 9.016163347387177 >50 0.0461186198184677 1.7738812197058456 >100 0.028921122599186888 3.0548514541832485 >500 0.0020012293913752076 0.7644445024731134 >1k 6.503197255862478E-4 0.5163802927415463 >5k 2.5012296037615473E-5 0.07864348523904827 >10k+ 7.503688811284643E-5 0.84837664575201 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26682 0.2523105297518767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21911 0.20719496354821115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12616 0.11929951440483007 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.45620754635622E-6 0.0 5 0.0 9.45620754635622E-6 0.0 9.45620754635622E-6 0.0 6 1.891241509271244E-5 9.45620754635622E-6 0.0 9.45620754635622E-6 9.45620754635622E-6 7 1.891241509271244E-5 9.45620754635622E-6 0.0 9.45620754635622E-6 9.45620754635622E-6 8 1.891241509271244E-5 9.45620754635622E-6 0.0 9.45620754635622E-6 2.836862263906866E-5 9 1.891241509271244E-5 9.45620754635622E-6 0.0 1.891241509271244E-5 3.782483018542488E-5 10-11 3.782483018542488E-5 9.45620754635622E-6 0.0 1.891241509271244E-5 3.782483018542488E-5 12-13 3.782483018542488E-5 9.45620754635622E-6 0.0 3.782483018542488E-5 3.782483018542488E-5 14-15 4.2552933958602996E-5 9.45620754635622E-6 0.0 4.72810377317811E-5 4.72810377317811E-5 16-17 5.200914150495921E-5 9.45620754635622E-6 0.0 4.72810377317811E-5 5.673724527813732E-5 18-19 5.673724527813732E-5 9.45620754635622E-6 0.0 4.72810377317811E-5 5.673724527813732E-5 20-21 5.673724527813732E-5 9.45620754635622E-6 0.0 8.510586791720599E-5 6.146534905131543E-5 22-23 6.619345282449354E-5 9.45620754635622E-6 0.0 3.735201980810707E-4 8.037776414402787E-5 24-25 9.929017923674031E-5 9.45620754635622E-6 0.0 8.841554055843067E-4 1.0401828300991843E-4 26-27 1.1347449055627464E-4 9.45620754635622E-6 0.0 0.0017683108111686131 1.2293069810263087E-4 28-29 1.2293069810263087E-4 1.891241509271244E-5 0.0 0.005111080178805537 1.2293069810263087E-4 30-31 1.4184311319534332E-4 1.891241509271244E-5 0.0 0.016359239055196262 1.2293069810263087E-4 32-33 1.4184311319534332E-4 1.891241509271244E-5 0.0 0.044127392515071306 1.6075552828805575E-4 34-35 1.5129932074169953E-4 1.891241509271244E-5 0.0 0.0869829251151577 1.7021173583441198E-4 36-37 1.6075552828805575E-4 1.891241509271244E-5 0.0 0.1549115920244076 1.7021173583441198E-4 38-39 1.7021173583441198E-4 2.8368622639068664E-5 0.0 0.24907177866724967 1.7021173583441198E-4 40-41 1.796679433807682E-4 3.782483018542488E-5 0.0 0.35022483079062217 1.891241509271244E-4 42-43 1.796679433807682E-4 3.782483018542488E-5 0.0 0.4511320215177894 1.9385225470030252E-4 44-45 1.796679433807682E-4 3.782483018542488E-5 0.0 0.5774764105446548 2.0330846224665873E-4 46-47 1.796679433807682E-4 3.782483018542488E-5 0.0 0.7251350913810073 2.1749277356619307E-4 48-49 1.796679433807682E-4 3.782483018542488E-5 0.0 0.8843965388767387 2.3640518865890553E-4 50-51 1.796679433807682E-4 5.200914150495921E-5 0.0 1.0542347545130697 2.4113329243208363E-4 52-53 1.796679433807682E-4 5.673724527813732E-5 0.0 1.2198034924422205 2.6004570752479606E-4 54-55 1.796679433807682E-4 5.673724527813732E-5 0.0 1.40523026621872 2.836862263906866E-4 56-57 1.796679433807682E-4 5.673724527813732E-5 0.0 1.6210871158793934 2.9787053771022096E-4 58-59 1.796679433807682E-4 5.673724527813732E-5 0.0 1.8625371912642799 3.0259864148339907E-4 60-61 1.891241509271244E-4 6.146534905131543E-5 0.0 2.1086444488657468 3.0259864148339907E-4 62-63 1.891241509271244E-4 6.619345282449354E-5 0.0 2.3558108017124058 3.120548490297553E-4 64-65 1.891241509271244E-4 7.092155659767165E-5 0.0 2.6125090117657916 3.120548490297553E-4 66-67 1.891241509271244E-4 7.564966037084977E-5 0.0 2.8991786716373538 3.167829528029334E-4 68-69 2.0803656601983686E-4 7.564966037084977E-5 0.0 3.2148741605724562 3.262391603492896E-4 70-71 2.1749277356619307E-4 7.564966037084977E-5 0.0 3.554314186656459 3.829764056274269E-4 72-73 2.1749277356619307E-4 7.564966037084977E-5 0.0 3.893063909589578 4.160731320396737E-4 74-75 2.1749277356619307E-4 7.564966037084977E-5 0.0 4.227080800645746 4.160731320396737E-4 76-77 2.2694898111254929E-4 7.564966037084977E-5 0.0 4.594459191925457 4.2552933958602993E-4 78-79 2.2694898111254929E-4 8.510586791720599E-5 0.0 4.990319680334795 4.3498554713238615E-4 80-81 2.2694898111254929E-4 9.929017923674031E-5 0.0 5.4121989238079315 4.586260659982767E-4 82-83 2.2694898111254929E-4 1.0401828300991843E-4 0.0 5.842357076987903 5.200914150495921E-4 84-85 2.3167708488572742E-4 1.0401828300991843E-4 0.0 6.259338004952027 5.200914150495921E-4 86-87 2.3640518865890553E-4 1.0401828300991843E-4 0.0 6.691846025707267 5.248195188227703E-4 88 2.3640518865890553E-4 1.0401828300991843E-4 0.0 7.0353333086211105 5.295476225959483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17080 0.0 49.071033 1 GTATCAA 30460 0.0 40.39563 1 CAACGCA 36725 0.0 32.50838 5 ATCAACG 37065 0.0 32.21688 3 TATCAAC 39245 0.0 31.009592 2 TCAACGC 39400 0.0 30.414757 4 AACGCAG 39915 0.0 30.250853 6 ACGCAGA 44790 0.0 26.654585 7 GTACTAG 4590 0.0 26.173325 1 CGCAGAG 45975 0.0 25.875616 8 GTACATG 43695 0.0 24.422512 1 TACATGG 44900 0.0 23.332045 2 GCAGAGT 51050 0.0 22.980124 9 GTGGTAT 8055 0.0 22.895947 1 ACATGGG 44755 0.0 22.414333 3 GAGTACT 25905 0.0 21.173544 12-13 TGGTATC 8795 0.0 20.430233 2 TAGTACT 6815 0.0 20.24143 4 CTGTGCG 6730 0.0 20.072979 9 CTAGTAC 6990 0.0 20.070293 3 >>END_MODULE