FastQCFastQC Report
Mon 6 Jun 2016
ERR1211309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7706574
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243220.3156006806656239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192880.25027982602905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107570.13958212819340993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130050.048.7173651
GTATCAA230300.041.8479921
CAACGCA288300.032.628955
ATCAACG289550.032.488513
TATCAAC292950.032.3360442
TCAACGC302900.031.133574
AACGCAG310350.030.6441846
GTGGTAT59100.027.8552531
ACGCAGA348000.026.979147
CGCAGAG358050.026.2460758
GTACTAG30100.025.939891
GTACATG313850.025.102631
TACATGG321900.024.1384852
GCAGAGT386300.023.6600279
TGGTATC67650.023.0829432
TACCTGG54500.022.956522
ACATGGG328650.022.8185753
GAGTACT209000.021.42699612-13
TAGTACT43200.020.6657264
CATGGGG228900.020.301654
Produced by FastQC (version 0.11.5)