##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7706574 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.029433961186903 31.0 31.0 34.0 27.0 34.0 2 31.043542305569247 31.0 31.0 34.0 27.0 34.0 3 31.08079673276348 31.0 31.0 34.0 27.0 34.0 4 34.67648088502102 37.0 35.0 37.0 31.0 37.0 5 34.71635385581193 37.0 35.0 37.0 31.0 37.0 6 34.781070291416135 37.0 35.0 37.0 31.0 37.0 7 34.70004673931633 37.0 35.0 37.0 30.0 37.0 8 34.69751266905372 37.0 35.0 37.0 30.0 37.0 9 36.192070043056745 39.0 35.0 39.0 30.0 39.0 10-11 36.113166434267676 39.0 35.0 39.0 30.0 39.0 12-13 36.02354386008621 39.0 35.0 39.0 30.0 39.0 14-15 37.092320725136744 40.0 36.0 41.0 30.0 41.0 16-17 37.088382788512774 40.0 36.0 41.0 30.0 41.0 18-19 37.08314200317807 40.0 36.0 41.0 30.0 41.0 20-21 37.04367816360421 40.0 36.0 41.0 30.0 41.0 22-23 36.915783200161314 40.0 36.0 41.0 30.0 41.0 24-25 36.79618005354909 39.5 36.0 41.0 30.0 41.0 26-27 36.642588587354126 39.0 36.0 41.0 29.0 41.0 28-29 36.49877202243175 39.0 35.5 41.0 29.0 41.0 30-31 36.30671709633879 39.0 35.0 40.5 28.5 41.0 32-33 36.186292313549444 39.0 35.0 40.0 28.0 41.0 34-35 36.03344339521038 39.0 35.0 40.0 27.5 41.0 36-37 35.93084391326158 38.5 35.0 40.0 27.0 41.0 38-39 35.77272481909601 38.0 35.0 40.0 27.0 41.0 40-41 35.59975295118168 38.0 34.0 40.0 26.0 41.0 42-43 35.43691379593578 38.0 34.0 40.0 26.0 41.0 44-45 35.286199937871224 38.0 34.0 40.0 25.0 41.0 46-47 35.11202792576832 38.0 33.5 40.0 24.0 41.0 48-49 34.86524608730157 38.0 33.0 40.0 24.0 41.0 50-51 34.205067647439705 37.5 32.5 39.5 23.0 40.5 52-53 34.46810813728642 38.0 33.0 39.5 24.0 40.5 54-55 34.81942267731419 38.0 33.0 40.0 24.0 41.0 56-57 34.71265532518081 38.0 33.0 40.0 24.0 41.0 58-59 34.480513455135835 37.5 33.0 40.0 23.0 41.0 60-61 34.20777118340783 37.0 33.0 40.0 23.0 41.0 62-63 33.92831016739734 36.5 32.0 40.0 23.0 41.0 64-65 33.64388916527629 36.0 32.0 39.0 22.0 41.0 66-67 33.419284885864975 35.5 32.0 39.0 22.0 41.0 68-69 33.081671894670706 35.0 31.5 39.0 22.0 40.5 70-71 32.74162624014251 35.0 31.0 38.0 21.0 40.0 72-73 32.38375885315576 35.0 31.0 37.0 20.0 40.0 74-75 32.01597655456238 35.0 30.5 37.0 20.0 39.0 76-77 31.667272772570534 35.0 30.0 36.0 20.0 39.0 78-79 31.30831034646524 34.0 30.0 36.0 19.0 38.5 80-81 30.97759004195639 34.0 29.5 35.5 18.0 37.0 82-83 30.67518757362221 34.0 29.0 35.0 18.0 37.0 84-85 30.38142902410332 34.0 29.0 35.0 18.0 36.5 86-87 30.12981384464744 34.0 29.0 35.0 17.0 36.0 88-89 29.870195498025453 34.0 29.0 35.0 16.5 36.0 90-91 29.612584852880154 34.0 28.0 35.0 15.0 35.5 92-93 29.37322395139526 34.0 27.0 35.0 14.0 35.0 94-95 29.150441882475924 33.5 27.0 35.0 11.5 35.0 96-97 28.947395950522242 33.0 26.5 35.0 10.5 35.0 98-99 28.70171252491704 33.0 25.5 35.0 10.0 35.0 100 26.998098895825823 31.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3378529837055737 1101 2 -0.31886030306440816 1101 3 -0.4105267528181784 1101 4 -0.22512270333739082 1101 5 -0.285878690596185 1101 6 -0.23628030750276707 1101 7 -0.3107609487437415 1101 8 -0.4350747693996553 1101 9 -0.48283386851475996 1101 10-11 -0.4808536736603628 1101 12-13 -0.5177290129531187 1101 14-15 -0.5162222134519538 1101 16-17 -0.5441868454921348 1101 18-19 -0.5957206275556928 1101 20-21 -0.628847234596634 1101 22-23 -0.5661906187778456 1101 24-25 -0.6218420947045828 1101 26-27 -0.753773720017783 1101 28-29 -0.6810656835223554 1101 30-31 -0.6553895472435727 1101 32-33 -0.8076727307318663 1101 34-35 -0.7588123190847327 1101 36-37 -0.8558990783954883 1101 38-39 -0.8741995728662317 1101 40-41 -0.8987629035508817 1101 42-43 -0.8739736386495665 1101 44-45 -1.1152124820951244 1101 46-47 -1.0091301030643294 1101 48-49 -1.034479753212537 1101 50-51 -0.9677180083746322 1101 52-53 -0.764523642763514 1101 54-55 -0.7704260453015621 1101 56-57 -0.7508716205398684 1101 58-59 -0.7958037613225812 1101 60-61 -0.8253547539257013 1101 62-63 -0.7937434430899302 1101 64-65 -0.8021488418121052 1101 66-67 -0.8220486606708732 1101 68-69 -0.8418986398568933 1101 70-71 -0.91698691846236 1101 72-73 -1.0147157143840317 1101 74-75 -1.077011961292854 1101 76-77 -1.0231934029573253 1101 78-79 -0.8956350508310535 1101 80-81 -0.99048776434811 1101 82-83 -1.086298483928676 1101 84-85 -1.1198867133577188 1101 86-87 -1.1265697171009776 1101 88-89 -1.186578604600701 1101 90-91 -1.224699616539528 1101 92-93 -1.287442797121141 1101 94-95 -1.3267146226208801 1101 96-97 -1.4430291870335488 1101 98-99 -1.4408635916420671 1101 100 -1.4013623452876693 1102 1 -0.41393498998262857 1102 2 -0.4746934747308913 1102 3 -0.4733159867118495 1102 4 -0.3975312254702601 1102 5 -0.33620941150175554 1102 6 -0.3397909475193899 1102 7 -0.3880998025877602 1102 8 -0.3381696761695423 1102 9 -0.43698867735106717 1102 10-11 -0.4196828929741656 1102 12-13 -0.47582658299911884 1102 14-15 -0.6273912177857923 1102 16-17 -0.6698704019507673 1102 18-19 -0.6259030562288785 1102 20-21 -0.6662422591652017 1102 22-23 -0.6946625579004362 1102 24-25 -0.7729705209720379 1102 26-27 -0.6792744937851793 1102 28-29 -0.8207280684492702 1102 30-31 -0.7729961485279517 1102 32-33 -0.7628591076664861 1102 34-35 -0.6488289934183129 1102 36-37 -0.7752136894342314 1102 38-39 -1.0054940022878753 1102 40-41 -0.9543278731477045 1102 42-43 -0.9238115209269537 1102 44-45 -1.0011684796555684 1102 46-47 -0.9701263002508824 1102 48-49 -1.0168903685606168 1102 50-51 -0.8710918817842028 1102 52-53 -0.7382491997063383 1102 54-55 -0.6940001238495697 1102 56-57 -0.6849790197633894 1102 58-59 -0.8375712218956011 1102 60-61 -0.821859347539494 1102 62-63 -0.8829607400777988 1102 64-65 -0.9688302035675704 1102 66-67 -0.7925088185904698 1102 68-69 -0.7761189553913397 1102 70-71 -0.8982424142481484 1102 72-73 -1.0029119469222891 1102 74-75 -0.9682085114036703 1102 76-77 -1.0008811858403988 1102 78-79 -0.8961152191198778 1102 80-81 -1.0731820491817672 1102 82-83 -1.1035635746890122 1102 84-85 -1.1494564257109587 1102 86-87 -1.1054658370589934 1102 88-89 -1.1964166117028547 1102 90-91 -1.1960662777498108 1102 92-93 -1.212968984625217 1102 94-95 -1.1632111260038158 1102 96-97 -1.2388124563493115 1102 98-99 -1.3091336845354853 1102 100 -1.3439353367748303 1103 1 -0.4813304667378624 1103 2 -0.44917473744065006 1103 3 -0.4067322515862095 1103 4 -0.3511779742714154 1103 5 -0.3443006446044592 1103 6 -0.4195435969390857 1103 7 -0.3506974868826802 1103 8 -0.43226426364415005 1103 9 -0.5220997778181555 1103 10-11 -0.5837367908477731 1103 12-13 -0.6486145802725574 1103 14-15 -0.7887292670727533 1103 16-17 -0.7332368302278809 1103 18-19 -0.7129564213768091 1103 20-21 -0.8503103477449088 1103 22-23 -0.7503840679448999 1103 24-25 -0.7537655036875748 1103 26-27 -0.8045566782435642 1103 28-29 -0.7601253660013043 1103 30-31 -0.7946949688101412 1103 32-33 -0.8515229688756847 1103 34-35 -0.8615886105323369 1103 36-37 -0.9231065541549484 1103 38-39 -0.9676899499563945 1103 40-41 -0.941075168459335 1103 42-43 -0.9187842121069636 1103 44-45 -0.9907472659906489 1103 46-47 -0.9518918672136891 1103 48-49 -1.0432248668302861 1103 50-51 -0.9705640983468555 1103 52-53 -0.841737602827628 1103 54-55 -0.7823555808676019 1103 56-57 -0.7074718756561467 1103 58-59 -0.7779213037170294 1103 60-61 -0.8550397381735308 1103 62-63 -0.8545724271194004 1103 64-65 -0.8460805122640807 1103 66-67 -0.8036138541614051 1103 68-69 -0.9100665762264555 1103 70-71 -0.8786141646455619 1103 72-73 -0.9394752687618819 1103 74-75 -0.90744626686676 1103 76-77 -0.7634579402845105 1103 78-79 -0.9153420244124248 1103 80-81 -1.0008476893030007 1103 82-83 -1.061292281227992 1103 84-85 -1.0115093325486058 1103 86-87 -1.12095649485806 1103 88-89 -1.1231016035424268 1103 90-91 -1.0090470572397585 1103 92-93 -1.0723850518102047 1103 94-95 -1.2556122100130729 1103 96-97 -1.3812682995631818 1103 98-99 -1.4758641457660069 1103 100 -1.4558528071827048 1104 1 -0.42884440359812004 1104 2 -0.450437091035397 1104 3 -0.5240203236722429 1104 4 -0.3873800624482442 1104 5 -0.39785411159678574 1104 6 -0.33028970911583855 1104 7 -0.36340198181835603 1104 8 -0.35084679869422786 1104 9 -0.4290384284597195 1104 10-11 -0.5158157002876109 1104 12-13 -0.575620298694453 1104 14-15 -0.6209263284380668 1104 16-17 -0.6205612599006827 1104 18-19 -0.6868834514639488 1104 20-21 -0.6793777739510602 1104 22-23 -0.6753870330133083 1104 24-25 -0.780314826979918 1104 26-27 -0.7985327852247721 1104 28-29 -0.7273857963162982 1104 30-31 -0.8695801735560948 1104 32-33 -0.8894326331455957 1104 34-35 -0.8755343415848316 1104 36-37 -0.8481869257386236 1104 38-39 -0.9086284152461275 1104 40-41 -0.9572429817126107 1104 42-43 -0.9208618893996245 1104 44-45 -0.9806928535315507 1104 46-47 -0.9448875228301503 1104 48-49 -0.8861206989963435 1104 50-51 -0.8238402252322956 1104 52-53 -0.7397455754493976 1104 54-55 -0.7669434783236255 1104 56-57 -0.7889039659916719 1104 58-59 -0.8452671845093747 1104 60-61 -0.77856342027723 1104 62-63 -0.8194350732467939 1104 64-65 -0.7745740105011336 1104 66-67 -0.8586172494737525 1104 68-69 -0.7669710659975522 1104 70-71 -0.767428129595288 1104 72-73 -0.7655559585515732 1104 74-75 -0.8394887063287264 1104 76-77 -0.8326621383601847 1104 78-79 -0.8676278776571067 1104 80-81 -0.9679571083567176 1104 82-83 -0.9929856330948468 1104 84-85 -1.0192737711021778 1104 86-87 -1.0422060437615173 1104 88-89 -1.0472301305170042 1104 90-91 -0.9866373828629555 1104 92-93 -1.0030848434794812 1104 94-95 -1.1030890183376094 1104 96-97 -1.126272603352966 1104 98-99 -0.9957667091203639 1104 100 -1.0061702933498218 1105 1 -0.3778647832718498 1105 2 -0.4393200941532065 1105 3 -0.373926634765823 1105 4 -0.3322604406974605 1105 5 -0.2685985299037341 1105 6 -0.29816929179895624 1105 7 -0.2950662256905474 1105 8 -0.28988602250403517 1105 9 -0.43964596975599335 1105 10-11 -0.4725589790673794 1105 12-13 -0.46896873682593565 1105 14-15 -0.5710915127444807 1105 16-17 -0.589946266517245 1105 18-19 -0.6099997509165149 1105 20-21 -0.5897353250770934 1105 22-23 -0.5585244136783984 1105 24-25 -0.5124471709397085 1105 26-27 -0.5181398276203666 1105 28-29 -0.6756356274377708 1105 30-31 -0.7706743327601941 1105 32-33 -0.7352262264454978 1105 34-35 -0.7409473027537317 1105 36-37 -0.7290890428275176 1105 38-39 -0.6545118904342075 1105 40-41 -0.7767694482646732 1105 42-43 -0.8818536192668134 1105 44-45 -0.8501892547108625 1105 46-47 -0.7897313557184091 1105 48-49 -0.7861543719721098 1105 50-51 -0.8577263638022856 1105 52-53 -0.6925765588251807 1105 54-55 -0.6428547052647744 1105 56-57 -0.6495059740185525 1105 58-59 -0.6145520584886128 1105 60-61 -0.6567773091177784 1105 62-63 -0.6344606370722374 1105 64-65 -0.5389345768662039 1105 66-67 -0.6540162644672023 1105 68-69 -0.6789502356856616 1105 70-71 -0.6775483665118713 1105 72-73 -0.6827761334439799 1105 74-75 -0.7117431512340424 1105 76-77 -0.8169558917562867 1105 78-79 -0.7226531075123823 1105 80-81 -0.7528357909720924 1105 82-83 -0.7730173771813575 1105 84-85 -0.7341009812192922 1105 86-87 -0.717737402795322 1105 88-89 -0.792971164807728 1105 90-91 -0.8364577741873696 1105 92-93 -0.9124901666820371 1105 94-95 -0.8878400751981026 1105 96-97 -0.9650351979362206 1105 98-99 -1.019493124541551 1105 100 -0.9611161458673045 1106 1 -0.5154075714032018 1106 2 -0.4678694502530085 1106 3 -0.5164672436741427 1106 4 -0.39768906784133407 1106 5 -0.33888486271808915 1106 6 -0.34968171531249936 1106 7 -0.4397736465470743 1106 8 -0.4269729995293545 1106 9 -0.5546174456299369 1106 10-11 -0.562738869849845 1106 12-13 -0.596482398728611 1106 14-15 -0.6468505310763675 1106 16-17 -0.7248637512665681 1106 18-19 -0.6464891313801076 1106 20-21 -0.6439432649072359 1106 22-23 -0.6562297326140936 1106 24-25 -0.7086922939084843 1106 26-27 -0.6890486568531955 1106 28-29 -0.7279056514286424 1106 30-31 -0.6965442059535008 1106 32-33 -0.779014343425878 1106 34-35 -0.7939409958258992 1106 36-37 -0.9135216504523953 1106 38-39 -0.8859933212009423 1106 40-41 -0.8220465553130794 1106 42-43 -0.8366873476571612 1106 44-45 -0.8028354957259367 1106 46-47 -0.8836427871999035 1106 48-49 -0.9742449860857008 1106 50-51 -0.8827924105751705 1106 52-53 -0.7530505611994087 1106 54-55 -0.8525539569483342 1106 56-57 -0.8037582072365979 1106 58-59 -0.7798124173728525 1106 60-61 -0.8024317208596301 1106 62-63 -0.8012647385175953 1106 64-65 -0.8049077475619058 1106 66-67 -0.8539838868843788 1106 68-69 -0.8551861095812896 1106 70-71 -0.8178405979896439 1106 72-73 -0.7558148909838565 1106 74-75 -0.7628166516489081 1106 76-77 -0.8109440524765077 1106 78-79 -0.7757009024183574 1106 80-81 -0.8258298084027693 1106 82-83 -0.7761992078996265 1106 84-85 -0.6953372163764939 1106 86-87 -0.7146042355646216 1106 88-89 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1107 46-47 -0.8487179938154625 1107 48-49 -0.8927351587340056 1107 50-51 -0.8649443636816443 1107 52-53 -0.7781899917464585 1107 54-55 -0.7516315931828714 1107 56-57 -0.6988915310876607 1107 58-59 -0.6588344261777408 1107 60-61 -0.6342484302674904 1107 62-63 -0.6917328135002165 1107 64-65 -0.7582312125199522 1107 66-67 -0.7188990872768386 1107 68-69 -0.5799917301263591 1107 70-71 -0.6854858690761247 1107 72-73 -0.6931695304687722 1107 74-75 -0.6953013217411161 1107 76-77 -0.7470146391820798 1107 78-79 -0.7050898381981199 1107 80-81 -0.6485862931830155 1107 82-83 -0.7434200480004307 1107 84-85 -0.7679793886909323 1107 86-87 -0.7909715776017734 1107 88-89 -0.8072492907626234 1107 90-91 -0.8392171116669864 1107 92-93 -0.8326281721154984 1107 94-95 -0.9178625346124818 1107 96-97 -0.8841789428574671 1107 98-99 -0.8676783355053495 1107 100 -0.9637110171157666 1108 1 -0.5016025774261479 1108 2 -0.49846430944270637 1108 3 -0.5185541949622419 1108 4 -0.35751921595497294 1108 5 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40-41 1.2727580578321493 2311 42-43 1.2778099847441027 2311 44-45 1.2962527090391234 2311 46-47 1.2880358783652284 2311 48-49 1.332777925304022 2311 50-51 1.306333833572907 2311 52-53 1.2288261088868708 2311 54-55 1.1735509857505022 2311 56-57 1.1273336168683272 2311 58-59 1.118554340382552 2311 60-61 1.0765440301685274 2311 62-63 1.1065050399945378 2311 64-65 1.1364967741919614 2311 66-67 1.0744715083832403 2311 68-69 1.0476122211646413 2311 70-71 1.0286978786798358 2311 72-73 1.0031897300294972 2311 74-75 1.1191402128407013 2311 76-77 1.0975977106504047 2311 78-79 1.0259093406765984 2311 80-81 1.0745354544079078 2311 82-83 1.1127826863224435 2311 84-85 1.2319872864938617 2311 86-87 1.2508713172471744 2311 88-89 1.3020724788368945 2311 90-91 1.3228809361318525 2311 92-93 1.3428379659044651 2311 94-95 1.339965391087265 2311 96-97 1.3596022865424437 2311 98-99 1.440692578265672 2311 100 1.4840484016627613 2312 1 0.5198757928329343 2312 2 0.48647162277389455 2312 3 0.579211202106972 2312 4 0.45366432314244776 2312 5 0.423091598026339 2312 6 0.4512967390540439 2312 7 0.49753668542444984 2312 8 0.4962128304326541 2312 9 0.6969587870551237 2312 10-11 0.6575270602723435 2312 12-13 0.6253469808009768 2312 14-15 0.8295045082150168 2312 16-17 0.7842399109171794 2312 18-19 0.7524982859851335 2312 20-21 0.8260667150616854 2312 22-23 0.8072183466450724 2312 24-25 0.820294179533839 2312 26-27 0.913842427249385 2312 28-29 0.9442608603682601 2312 30-31 0.958346524296573 2312 32-33 0.989476869315844 2312 34-35 1.012115194624542 2312 36-37 1.0502569915603388 2312 38-39 1.0941469201302567 2312 40-41 1.1049186423913397 2312 42-43 1.1526542447645127 2312 44-45 1.0566696894465935 2312 46-47 1.0978258714761466 2312 48-49 1.1170847499052243 2312 50-51 1.040719025359266 2312 52-53 0.9144379113198653 2312 54-55 0.8487071050930197 2312 56-57 0.7957100416156422 2312 58-59 0.817988384534722 2312 60-61 0.8723009094171488 2312 62-63 0.8445032613571826 2312 64-65 0.8148175850997674 2312 66-67 0.8666328125824236 2312 68-69 0.967224162007625 2312 70-71 0.924246292249201 2312 72-73 0.8566596669732505 2312 74-75 0.8738950482044707 2312 76-77 0.8966483606600519 2312 78-79 0.8968846352595818 2312 80-81 0.9599851883978516 2312 82-83 0.9723910029082461 2312 84-85 1.025623469471256 2312 86-87 0.9537464322726947 2312 88-89 1.0459021024222643 2312 90-91 1.1155863387428333 2312 92-93 1.1240062136715316 2312 94-95 1.11149785869139 2312 96-97 1.1268141935416587 2312 98-99 1.2000786111647912 2312 100 1.1925794922944064 2313 1 0.5061921519909021 2313 2 0.579481227884262 2313 3 0.6151442320294187 2313 4 0.482712417390303 2313 5 0.46323030271949506 2313 6 0.4905833220634719 2313 7 0.5418832406058058 2313 8 0.534058213031031 2313 9 0.6067047474493918 2313 10-11 0.675205369911346 2313 12-13 0.6873633101142715 2313 14-15 0.8029872304305101 2313 16-17 0.8228980269883834 2313 18-19 0.7744772874533652 2313 20-21 0.7697738551974282 2313 22-23 0.6995176018828175 2313 24-25 0.7142062915676846 2313 26-27 0.8022887249931614 2313 28-29 0.8141972416165828 2313 30-31 0.8847397231015606 2313 32-33 0.8829156662414022 2313 34-35 0.8670635087177132 2313 36-37 0.9646932967615385 2313 38-39 0.9827151674827164 2313 40-41 0.9653386735347453 2313 42-43 0.860201625904871 2313 44-45 0.9713369683009461 2313 46-47 1.0516516106156573 2313 48-49 1.088160793857604 2313 50-51 1.0682636666404832 2313 52-53 0.9347301652789852 2313 54-55 0.9646199860691951 2313 56-57 0.9899759356116462 2313 58-59 0.9826227765824811 2313 60-61 0.9398742078586722 2313 62-63 0.9610210626012972 2313 64-65 0.9300293572092997 2313 66-67 0.8545777413364277 2313 68-69 0.9828201490885533 2313 70-71 0.9430682504036838 2313 72-73 0.9667780265193429 2313 74-75 0.9473319567617473 2313 76-77 0.9624316680045055 2313 78-79 0.9306231258106408 2313 80-81 0.9473760365782695 2313 82-83 0.9455400228453605 2313 84-85 1.0012954462532377 2313 86-87 1.059780789864302 2313 88-89 1.0859133023464231 2313 90-91 1.1345650193635493 2313 92-93 1.1611517619359866 2313 94-95 1.2236899537922348 2313 96-97 1.3105682630023487 2313 98-99 1.2632633651373624 2313 100 1.2729318287562137 2314 1 0.25114725333667565 2314 2 0.16562470979808808 2314 3 0.2881302209544607 2314 4 0.21150791144016523 2314 5 0.13436853199882393 2314 6 0.23860330526789397 2314 7 0.26361623046380345 2314 8 0.2926033452249257 2314 9 0.38271281852416905 2314 10-11 0.3896314283500786 2314 12-13 0.40550189376947543 2314 14-15 0.5031797877039494 2314 16-17 0.5321201343094017 2314 18-19 0.5068639685032394 2314 20-21 0.5190115630387027 2314 22-23 0.5588537141641936 2314 24-25 0.6030453745017752 2314 26-27 0.5857866321724927 2314 28-29 0.6713240018490509 2314 30-31 0.6494410064351825 2314 32-33 0.6626887612665442 2314 34-35 0.6831678549150766 2314 36-37 0.7297690053827424 2314 38-39 0.7757332801608854 2314 40-41 0.7595433479627758 2314 42-43 0.6623845076980572 2314 44-45 0.8345721375780499 2314 46-47 0.65830017804155 2314 48-49 0.512835113449917 2314 50-51 0.6588573857788091 2314 52-53 0.6091173733995774 2314 54-55 0.5949423699630287 2314 56-57 0.5521615770083699 2314 58-59 0.5753768569000499 2314 60-61 0.3877530556908084 2314 62-63 0.513343888724485 2314 64-65 0.5748165102984686 2314 66-67 0.5945198034676196 2314 68-69 0.6874793659133687 2314 70-71 0.6176692060588636 2314 72-73 0.6387495775018763 2314 74-75 0.7039846307106465 2314 76-77 0.7501540854232331 2314 78-79 0.7570635414670832 2314 80-81 0.7431334711921807 2314 82-83 0.6739825968862725 2314 84-85 0.730259217382752 2314 86-87 0.7886182716058379 2314 88-89 0.7220204869644498 2314 90-91 0.641196752806227 2314 92-93 0.8388927267319382 2314 94-95 0.8294978574734877 2314 96-97 0.8173336341497013 2314 98-99 0.8710333823285303 2314 100 0.9094359542555743 2315 1 0.21958226007708603 2315 2 0.2328133355580917 2315 3 0.2715649125308559 2315 4 0.24387235286025088 2315 5 0.19655358752623897 2315 6 0.2227097694281852 2315 7 0.21380768040560838 2315 8 0.17812884755538505 2315 9 0.0909638807147104 2315 10-11 0.2459333596712483 2315 12-13 0.2590661670305323 2315 14-15 0.3889071950301215 2315 16-17 0.27199801508412236 2315 18-19 0.3670641396089991 2315 20-21 0.3646988215918441 2315 22-23 0.3036569962022213 2315 24-25 0.42857535743023334 2315 26-27 0.5187603695454399 2315 28-29 0.42367948695184054 2315 30-31 0.42377821797716564 2315 32-33 0.46055251518023965 2315 34-35 0.3642040044226391 2315 36-37 0.507488119504508 2315 38-39 0.5073392642571051 2315 40-41 0.5882166040617278 2315 42-43 0.5726952569765942 2315 44-45 0.5423274384806476 2315 46-47 0.5831662113814176 2315 48-49 0.4209846108657658 2315 50-51 0.41073867344725556 2315 52-53 0.13661844622568964 2315 54-55 0.3668235220036209 2315 56-57 0.3376407634012466 2315 58-59 0.36522349204120985 2315 60-61 0.4510064001371674 2315 62-63 0.47424306043832587 2315 64-65 0.48638546408664496 2315 66-67 0.37208456772103915 2315 68-69 0.4050706502916199 2315 70-71 0.3289068741691068 2315 72-73 0.38475515414859274 2315 74-75 0.12808168337094727 2315 76-77 0.3561695760760344 2315 78-79 0.4167444627841874 2315 80-81 0.5299204784727642 2315 82-83 0.4390709764865939 2315 84-85 0.4715409074658794 2315 86-87 0.48338681486116997 2315 88-89 0.4556333608582541 2315 90-91 0.5417882658979281 2315 92-93 0.7208905021423568 2315 94-95 0.7201524833786799 2315 96-97 0.6845532786837225 2315 98-99 0.8916889561651189 2315 100 0.8712771785282385 2316 1 -0.08541172114916051 2316 2 -0.04694073756660089 2316 3 -0.05272017218129221 2316 4 -0.18761355806373814 2316 5 -0.23112570947731115 2316 6 -0.35019321910106527 2316 7 -0.3287801689200336 2316 8 -0.2485523666896512 2316 9 -0.29755690177694305 2316 10-11 -0.2406996126566625 2316 12-13 -0.156022696799198 2316 14-15 -0.3215712605140837 2316 16-17 -0.1917141170245742 2316 18-19 -0.1264031910359762 2316 20-21 -0.09720067784687103 2316 22-23 -0.15089518716037986 2316 24-25 -0.18227357513624298 2316 26-27 -0.36048613206425273 2316 28-29 -0.25472418182214795 2316 30-31 -0.8014933916375426 2316 32-33 -0.68010091621057 2316 34-35 -0.7889997930694008 2316 36-37 -0.4435488589304484 2316 38-39 -0.355720775337538 2316 40-41 -0.17792888329073264 2316 42-43 -0.1268140720440627 2316 44-45 -0.3528537899348976 2316 46-47 -0.47912679003245984 2316 48-49 -0.30966794246198504 2316 50-51 -0.1622192363773678 2316 52-53 -0.4540668016396765 2316 54-55 -0.40638224096529285 2316 56-57 -0.42045300913496675 2316 58-59 -0.7781455453981394 2316 60-61 -0.3087349991589363 2316 62-63 -0.32701961263393997 2316 64-65 -0.6567646384456083 2316 66-67 -0.925436777403803 2316 68-69 -0.9273689791822335 2316 70-71 -0.6338754319986606 2316 72-73 -0.8476559031823676 2316 74-75 -0.7018621825514408 2316 76-77 -0.43083464049023235 2316 78-79 -0.3593246376806256 2316 80-81 -0.3794181916833068 2316 82-83 -0.16515631870380432 2316 84-85 -0.1290865107017538 2316 86-87 0.03315945509876883 2316 88-89 -0.043511586514473066 2316 90-91 -0.6857127356578765 2316 92-93 -0.7291223880556892 2316 94-95 -0.3113314078550573 2316 96-97 -0.06043563262385021 2316 98-99 0.05895117127957761 2316 100 -0.06495157984243605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 228.0 1 562.0 2 360.0 3 307.0 4 315.0 5 294.0 6 319.0 7 776.0 8 1993.0 9 3688.0 10 6980.0 11 13591.0 12 21767.0 13 28059.0 14 32187.0 15 35872.0 16 40025.0 17 46093.0 18 53031.0 19 60763.0 20 71501.0 21 84000.0 22 92503.0 23 96005.0 24 98017.0 25 102424.0 26 112331.0 27 126876.0 28 147702.0 29 172753.0 30 205466.0 31 249042.0 32 307665.0 33 393155.0 34 527480.0 35 720598.0 36 1031461.0 37 1384386.0 38 1176971.0 39 258896.0 40 132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83524750850917 19.177288859261658 13.712882030323609 23.274581601905556 2 15.788559577711514 19.854937383533894 40.25970196367784 24.09680107507676 3 19.124437718845147 25.294748235611703 29.289053394971205 26.29176065057195 4 13.095769894556893 16.421589685300756 37.65646255943066 32.826177860711695 5 13.925822771010218 37.29463495417297 34.169286157408244 14.61025611740856 6 31.452921772341536 36.44410056821796 17.63650148058621 14.466476178854295 7 27.997967532447298 32.12085444645118 21.705938190968865 18.175239830132657 8 26.354572565928493 34.78399766134524 19.850799122589073 19.010630650137198 9 26.55027693842947 16.04739787169335 20.407164586875567 36.995160603001615 10-11 25.40781575248542 25.94342035098869 27.454682955931442 21.194080940594446 12-13 25.81837558368243 23.6233110163741 27.83991500279246 22.71839839715101 14-15 23.323926246745085 25.06739574832742 25.511132288810106 26.097545716117388 16-17 22.575845589977213 28.001971793871604 26.320722203580253 23.101460412570933 18-19 22.357639598097723 27.16907891239725 28.48251690564557 21.99076458385946 20-21 23.371179830699145 26.26005390557042 28.313529898326145 22.05523636540429 22-23 23.414059449707075 26.012886709189626 28.079362343845286 22.493691497258013 24-25 22.94816772604819 26.2843024880777 28.29346247822238 22.47406730765173 26-27 22.794270872216405 26.444820823560494 28.064306297872836 22.69660200635026 28-29 22.547447252611363 26.44966509826348 28.348645738973005 22.654241910152145 30-31 23.004940219430726 26.32301480676723 28.161282485040655 22.510762488761387 32-33 22.919210520918174 26.6111343770373 27.850813025122267 22.618842076922256 34-35 23.059689852590697 26.528416613295597 28.08115284364104 22.330740690472663 36-37 23.07566589590686 26.314823739402055 27.89410571579246 22.715404648898623 38-39 22.977227509150254 26.402039136105028 28.07487304405804 22.54586031068668 40-41 22.930542328275024 26.150664133000962 28.270130500055796 22.64866303866822 42-43 22.845035653162668 26.189111718090086 28.015049345244293 22.95080328350295 44-45 22.868714758483833 26.530450215014717 27.864280213881248 22.736554812620206 46-47 22.879454192850364 26.477157075685533 27.551234176904977 23.09215455455913 48-49 23.148934623219773 26.39951856473084 27.548055887000338 22.90349092504905 50-51 22.996896733374513 26.60501462699618 27.32648760267541 23.071601036953897 52-53 22.847754370554746 26.795963089883283 27.257518081276395 23.098764458285576 54-55 22.796396897920914 26.730054453404644 27.282384527747332 23.191164120927102 56-57 23.01110626120279 26.643134369090255 27.11448573560025 23.231273634106703 58-59 22.7900878403638 26.593818308318852 27.42817096170258 23.18792288961476 60-61 22.8582634139806 26.788800128992 27.224024812785686 23.12891164424171 62-63 22.853436357118827 26.680793617593917 27.190381728883807 23.275388296403452 64-65 22.628328611346333 26.574375595793153 27.283670082574456 23.513625710286053 66-67 22.83512965645564 26.468123822311107 27.17204975056429 23.524696770668967 68-69 22.613021254988126 26.52641265898039 27.29786423135251 23.562701854678977 70-71 22.675864484781552 26.283353413311783 27.345587410940876 23.69519469096579 72-73 22.826344232821917 26.491968691966587 27.166287475553176 23.51539959965832 74-75 22.77093481902193 26.60144968365416 27.131812886117316 23.49580261120659 76-77 22.69711282490895 26.537870877307228 27.05282164510831 23.71219465267551 78-79 22.68432515045285 26.389054343762258 27.144153958704496 23.782466547080393 80-81 22.5761713508235 26.658813970165205 27.14535525596376 23.619659423047533 82-83 22.836341863078125 26.353776716998915 27.391314254244055 23.418567165678905 84-85 22.8517048113212 26.40149081729049 27.079307366681537 23.667497004706778 86-87 22.465219601272615 26.58503905674377 27.328853692847094 23.620887649136517 88-89 22.703663851728376 26.145563112523046 27.303315173153177 23.8474578625954 90-91 22.783657748522273 26.21952890143875 27.2717103107818 23.725103039257174 92-93 22.775995103378918 26.275511810721987 27.22665721139715 23.721835874501945 94-95 22.455574659505285 26.05659198811515 27.45008155251047 24.0377517998691 96-97 22.57682196701452 25.979461940259114 27.37106381503345 24.072652277692914 98-99 22.489275770439054 26.084667762351994 27.27381815298778 24.152238314221176 100 22.760196527508185 25.903332226384972 26.9199837439696 24.41648750213724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1637.0 1 1212.5 2 1227.5 3 1574.5 4 1205.5 5 1123.0 6 1726.0 7 3024.5 8 4950.0 9 6037.0 10 5383.5 11 4731.5 12 4988.0 13 5445.5 14 6039.0 15 6758.0 16 7376.0 17 8002.0 18 8705.0 19 9295.5 20 10002.5 21 11071.0 22 12552.5 23 14728.5 24 17387.5 25 20725.0 26 25209.0 27 30804.0 28 38136.0 29 48596.5 30 62110.5 31 76413.5 32 94626.0 33 115308.5 34 135596.0 35 156086.5 36 179224.5 37 205361.0 38 220111.0 39 223937.0 40 224930.0 41 225023.0 42 230569.0 43 242666.5 44 255140.5 45 273081.5 46 291478.5 47 304483.0 48 316664.0 49 322013.0 50 317768.0 51 312500.5 52 304903.0 53 295876.5 54 286688.5 55 265836.0 56 241741.0 57 214162.5 58 186726.0 59 166048.0 60 141447.0 61 115203.0 62 92763.5 63 73303.5 64 55399.5 65 40145.0 66 29631.5 67 22534.5 68 17373.5 69 13216.5 70 10094.0 71 7758.0 72 5884.0 73 4462.0 74 3383.5 75 2410.0 76 1688.5 77 1158.0 78 812.0 79 570.0 80 362.5 81 243.0 82 186.5 83 137.0 84 100.5 85 83.5 86 60.5 87 39.0 88 43.0 89 44.5 90 33.0 91 21.0 92 17.0 93 17.0 94 12.0 95 9.5 96 8.5 97 4.5 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025303072415836143 2 0.11054198662077339 3 0.01756941541079084 4 0.01785488597137976 5 0.019645564942346625 6 0.04598671212396066 7 0.046998835020594106 8 0.03892780371667099 9 0.03263447544914251 10-11 0.036981413530837434 12-13 0.036222321358362355 14-15 0.03182996750566464 16-17 0.03800651236204311 18-19 0.03711766084384578 20-21 0.04748543256705249 22-23 0.0477060234547803 24-25 0.06678064727594908 26-27 0.058768007677600965 28-29 0.05114464611641956 30-31 0.04902956878114711 32-33 0.0437029476392493 34-35 0.04451394355001328 36-37 0.040699018785779514 38-39 0.045519578479360605 40-41 0.054025303591453216 42-43 0.05595222987542844 44-45 0.058949670761612095 46-47 0.060461367139276155 48-49 0.05974120277051774 50-51 0.05458326877805884 52-53 0.06347178396003204 54-55 0.051040838639841775 56-57 0.055173673801095016 58-59 0.05366197742343096 60-61 0.04893224927185544 62-63 0.05547212029625616 64-65 0.05361007368514207 66-67 0.051748027074027966 68-69 0.05716547975793135 70-71 0.05784022835568697 72-73 0.060377023564556695 74-75 0.050969470999694545 76-77 0.051812906746889084 78-79 0.06153836970877072 80-81 0.06177842449835686 82-83 0.05264985452679751 84-85 0.05826194622928425 86-87 0.056043061417433995 88-89 0.06390647776820155 90-91 0.06984945580227997 92-93 0.07465703956128884 94-95 0.07803727051735311 96-97 0.073969315028961 98-99 0.08266319119235085 100 0.12635965086431403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7706574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.745368267644594 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15683812359751 42.822139270112444 2 14.586396950564318 16.845937272167692 3 5.095867364589739 8.827882128339178 4 2.2573125834204153 5.213973856992004 5 1.163145519989763 3.3583133200334903 6 0.6886845520619916 2.386100584743454 7 0.46198809112057104 1.8674370719916467 8 0.30591917409540353 1.4132332294618206 9 0.20233484355047845 1.0515510048778767 >10 1.0060826693213596 9.995739102883922 >50 0.050396458345209935 2.0038839883918707 >100 0.023384557843645497 2.3857855390314335 >500 0.0010994126443138706 0.41627314949226546 >1k 4.580823794025695E-4 0.4530016410747203 >5k 0.0 0.0 >10k+ 9.161647583278635E-5 0.9587488404061073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24322 0.3156006806656239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19288 0.25027982602905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10757 0.13958212819340993 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.487967286111832E-5 2 0.0 0.0 0.0 0.0 7.785560743334198E-5 3 0.0 0.0 0.0 0.0 7.785560743334198E-5 4 0.0 0.0 0.0 0.0 1.038074765777893E-4 5 0.0 0.0 0.0 0.0 1.038074765777893E-4 6 1.2975934572223662E-5 0.0 0.0 0.0 1.4273528029446028E-4 7 1.2975934572223662E-5 0.0 0.0 0.0 1.5571121486668396E-4 8 1.2975934572223662E-5 0.0 0.0 0.0 1.5571121486668396E-4 9 1.2975934572223662E-5 0.0 0.0 0.0 1.5571121486668396E-4 10-11 5.190373828889465E-5 0.0 0.0 6.487967286111831E-6 1.621991821527958E-4 12-13 5.190373828889465E-5 0.0 0.0 1.9463901858335492E-5 2.076149531555786E-4 14-15 5.839170557500648E-5 0.0 0.0 3.243983643055916E-5 2.4005478958613775E-4 16-17 7.785560743334198E-5 0.0 0.0 3.892780371667099E-5 2.5951869144447327E-4 18-19 9.083154200556564E-5 1.2975934572223662E-5 0.0 4.5415771002782815E-5 2.8547056058892057E-4 20-21 9.083154200556564E-5 2.5951869144447324E-5 0.0 7.136764014723014E-5 2.9844649516114425E-4 22-23 1.232713784361248E-4 2.5951869144447324E-5 0.0 2.9844649516114425E-4 3.373742988778152E-4 24-25 1.4922324758057212E-4 2.5951869144447324E-5 0.0 0.001031586798491781 3.763021025944862E-4 26-27 1.621991821527958E-4 2.5951869144447324E-5 0.0 0.0021150773352724573 4.0225397173893355E-4 28-29 1.686871494389076E-4 3.243983643055916E-5 0.0 0.005404476749331155 4.0225397173893355E-4 30-31 1.7517511672501945E-4 3.892780371667099E-5 0.0 0.016680563892593515 4.0874193902504534E-4 32-33 1.816630840111313E-4 6.487967286111832E-5 0.0 0.042645408971613064 4.152299063111572E-4 34-35 2.205908877278023E-4 6.487967286111832E-5 0.0 0.08481719633133997 4.152299063111572E-4 36-37 2.5951869144447327E-4 6.487967286111832E-5 0.0 0.1527786536533614 4.2820584088338085E-4 38-39 2.5951869144447327E-4 6.487967286111832E-5 0.0 0.24564741738676615 4.5415771002782815E-4 40-41 2.8547056058892057E-4 6.487967286111832E-5 0.0 0.3427852635944325 4.6713364460005183E-4 42-43 2.8547056058892057E-4 6.487967286111832E-5 0.0 0.44146724601619347 4.6713364460005183E-4 44-45 3.0493446244725603E-4 6.487967286111832E-5 0.0 0.5659972901058239 4.6713364460005183E-4 46-47 3.2439836430559155E-4 6.487967286111832E-5 0.0 0.7137153292760181 4.8659754645838735E-4 48-49 3.2439836430559155E-4 6.487967286111832E-5 0.0 0.8699326055910188 5.060614483167229E-4 50-51 3.2439836430559155E-4 6.487967286111832E-5 0.0 1.0299193908992503 5.190373828889465E-4 52-53 3.2439836430559155E-4 6.487967286111832E-5 0.0 1.1872201577510317 5.449892520333938E-4 54-55 3.308863315917034E-4 6.487967286111832E-5 0.0 1.363478765012832 5.839170557500648E-4 56-57 3.373742988778152E-4 7.136764014723014E-5 0.0 1.5683882358101018 6.228448594667358E-4 58-59 3.373742988778152E-4 9.083154200556563E-5 0.0 1.796518141524366 6.487967286111831E-4 60-61 3.373742988778152E-4 1.038074765777893E-4 0.0 2.0323103366035284 6.487967286111831E-4 62-63 3.4386226616392706E-4 1.038074765777893E-4 0.0 2.2681090196499767 6.487967286111831E-4 64-65 3.503502334500389E-4 1.2975934572223663E-4 0.0 2.5120371257059233 6.747485977556304E-4 66-67 3.763021025944862E-4 1.2975934572223663E-4 0.0 2.787081522866062 6.877245323278541E-4 68-69 4.0225397173893355E-4 1.2975934572223663E-4 0.0 3.096064476899852 7.007004669000778E-4 70-71 4.0225397173893355E-4 1.2975934572223663E-4 0.0 3.424959002534719 7.266523360445252E-4 72-73 4.0225397173893355E-4 1.2975934572223663E-4 0.0 3.750836623381544 7.590921724750842E-4 74-75 4.0225397173893355E-4 1.2975934572223663E-4 0.0 4.072192131029949 7.785560743334198E-4 76-77 4.2820584088338085E-4 1.2975934572223663E-4 0.0 4.426668711673955 7.785560743334198E-4 78-79 4.2820584088338085E-4 1.2975934572223663E-4 0.0 4.813105019169348 7.850440416195315E-4 80-81 4.2820584088338085E-4 1.2975934572223663E-4 0.0 5.224331849664974 8.304598126223145E-4 82-83 4.346938081694927E-4 1.2975934572223663E-4 0.0 5.636603242893664 8.693876163389854E-4 84-85 4.4118177545560453E-4 1.2975934572223663E-4 0.0 6.042944634022849 8.693876163389854E-4 86-87 4.4118177545560453E-4 1.3624731300834845E-4 0.0 6.464889586475131 9.018274527695445E-4 88 4.4118177545560453E-4 1.4273528029446028E-4 0.0 6.79746927752851 9.342672892001037E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13005 0.0 48.717365 1 GTATCAA 23030 0.0 41.847992 1 CAACGCA 28830 0.0 32.62895 5 ATCAACG 28955 0.0 32.48851 3 TATCAAC 29295 0.0 32.336044 2 TCAACGC 30290 0.0 31.13357 4 AACGCAG 31035 0.0 30.644184 6 GTGGTAT 5910 0.0 27.855253 1 ACGCAGA 34800 0.0 26.97914 7 CGCAGAG 35805 0.0 26.246075 8 GTACTAG 3010 0.0 25.93989 1 GTACATG 31385 0.0 25.10263 1 TACATGG 32190 0.0 24.138485 2 GCAGAGT 38630 0.0 23.660027 9 TGGTATC 6765 0.0 23.082943 2 TACCTGG 5450 0.0 22.95652 2 ACATGGG 32865 0.0 22.818575 3 GAGTACT 20900 0.0 21.426996 12-13 TAGTACT 4320 0.0 20.665726 4 CATGGGG 22890 0.0 20.30165 4 >>END_MODULE