FastQCFastQC Report
Mon 6 Jun 2016
ERR1211309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7706574
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222030.28810467530708195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187770.2436491234626437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107050.1389073795956543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138150.047.658461
GTATCAA239150.040.1287961
CAACGCA290400.032.3217475
ATCAACG293900.031.9436743
TATCAAC303550.031.4209372
TCAACGC304300.030.8831794
AACGCAG310500.030.4862446
ACGCAGA354950.026.33817
GTGGTAT63550.026.2886121
CGCAGAG365700.025.4740898
GCAGAGT400550.022.9758999
TGGTATC70700.022.69442
GTACATG324900.022.5902081
TACCTGG54200.022.159132
TACATGG331800.021.9021642
GAGTACT206200.021.54004112-13
ACATGGG335900.020.89583
GTACTTT222200.019.89401614-15
CAGAGTA390750.019.70921110-11
AGAGTAC374600.019.39450610-11
Produced by FastQC (version 0.11.5)