##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211309_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7706574 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.103303102000964 33.0 31.0 34.0 30.0 34.0 2 32.23753187862726 34.0 31.0 34.0 30.0 34.0 3 32.30235471689495 34.0 31.0 34.0 30.0 34.0 4 35.94478233778071 37.0 35.0 37.0 35.0 37.0 5 35.844740477415776 37.0 35.0 37.0 35.0 37.0 6 35.8604539449047 37.0 35.0 37.0 35.0 37.0 7 35.77270808014041 37.0 35.0 37.0 33.0 37.0 8 35.75165942739277 37.0 35.0 37.0 33.0 37.0 9 37.36379213383275 39.0 37.0 39.0 34.0 39.0 10-11 37.314044995869764 39.0 37.0 39.0 34.0 39.0 12-13 37.24847967462584 39.0 37.0 39.0 33.0 39.0 14-15 38.485634602353784 40.0 38.0 41.0 34.0 41.0 16-17 38.46854393404904 40.0 38.0 41.0 34.0 41.0 18-19 38.46658307569615 40.0 38.0 41.0 34.0 41.0 20-21 38.4326493199183 40.0 38.0 41.0 34.0 41.0 22-23 38.32762937201407 40.0 38.0 41.0 34.0 41.0 24-25 38.2384385720555 40.0 38.0 41.0 33.0 41.0 26-27 38.14001708152027 40.0 38.0 41.0 33.0 41.0 28-29 38.03741260383667 40.0 37.0 41.0 33.0 41.0 30-31 38.046880948655 40.0 37.0 41.0 33.0 41.0 32-33 37.98792381673101 40.0 37.0 41.0 33.0 41.0 34-35 37.86212978685471 40.0 37.0 41.0 33.0 41.0 36-37 37.719011067693636 40.0 37.0 41.0 33.0 41.0 38-39 37.60731882935271 40.0 37.0 41.0 32.5 41.0 40-41 37.49371860959228 40.0 36.5 41.0 32.0 41.0 42-43 37.38394181383323 40.0 36.0 41.0 32.0 41.0 44-45 37.221580367618614 39.5 36.0 41.0 31.0 41.0 46-47 36.9803461953392 39.0 36.0 41.0 31.0 41.0 48-49 35.418601054113026 39.0 35.0 40.5 24.0 41.0 50-51 36.147464619168 39.0 35.0 40.0 29.0 41.0 52-53 36.23837044839899 39.0 35.0 40.0 29.5 41.0 54-55 36.064357131975896 38.0 35.0 40.0 29.0 41.0 56-57 35.84136303628564 38.0 34.5 40.0 28.5 41.0 58-59 35.59435165353632 38.0 34.0 40.0 28.0 41.0 60-61 35.335100850261085 37.5 34.0 40.0 28.0 41.0 62-63 35.01314274021115 37.0 34.0 40.0 27.5 41.0 64-65 34.67249020122301 36.0 33.5 39.0 27.0 41.0 66-67 34.330876664520446 36.0 33.0 39.0 26.0 40.0 68-69 33.9713303213594 35.0 33.0 38.5 26.0 40.0 70-71 33.61708626946293 35.0 32.5 38.0 26.0 40.0 72-73 33.246042404835144 35.0 32.0 37.0 26.0 39.5 74-75 32.88762367038842 35.0 32.0 37.0 25.0 39.0 76-77 31.965711028013224 34.0 30.5 36.0 24.5 38.0 78-79 32.407047865886966 34.5 31.5 36.0 25.0 38.0 80-81 32.288629681619874 35.0 32.0 35.5 25.0 37.0 82-83 32.02957390664126 34.0 31.0 35.0 25.0 37.0 84-85 31.761066979957633 34.0 31.0 35.0 24.0 36.0 86-87 31.50906693428234 34.0 31.0 35.0 24.0 36.0 88-89 31.288535346575536 34.0 31.0 35.0 23.0 36.0 90-91 31.075289421732666 34.0 30.5 35.0 23.0 35.0 92-93 30.89171621527283 34.0 30.0 35.0 22.0 35.0 94-95 30.703771027177574 34.0 30.0 35.0 20.0 35.0 96-97 30.520801590953386 34.0 30.0 35.0 20.0 35.0 98-99 30.304483678480217 34.0 29.0 35.0 19.5 35.0 100 27.839615891575168 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29184595495781807 1101 2 -0.2915474962645277 1101 3 -0.3137613264363601 1101 4 -0.1910131210857955 1101 5 -0.20962307528497348 1101 6 -0.25947753278827435 1101 7 -0.2588239947665727 1101 8 -0.24885702856486347 1101 9 -0.3139705004818225 1101 10-11 -0.3298555706350257 1101 12-13 -0.4273058061592039 1101 14-15 -0.5353588268385678 1101 16-17 -0.4912669848323432 1101 18-19 -0.5443220528411814 1101 20-21 -0.5721934198739902 1101 22-23 -0.6141138730525455 1101 24-25 -0.646710825049297 1101 26-27 -0.6893221017504771 1101 28-29 -0.689692175057381 1101 30-31 -0.5915342706295235 1101 32-33 -0.6302292541479062 1101 34-35 -0.6975860052515443 1101 36-37 -0.6469989835814332 1101 38-39 -0.6804447735378218 1101 40-41 -0.6739692498325383 1101 42-43 -0.6711685865676387 1101 44-45 -0.6878993096835231 1101 46-47 -0.6239252735828771 1101 48-49 0.5322306771995287 1101 50-51 -0.3246564180661409 1101 52-53 -0.6664931026868288 1101 54-55 -0.65364799796928 1101 56-57 -0.7367214777008471 1101 58-59 -0.8417248616737893 1101 60-61 -0.7686426210060944 1101 62-63 -0.8177906844584442 1101 64-65 -0.7898468446178484 1101 66-67 -0.7703008418679715 1101 68-69 -0.8231687210988099 1101 70-71 -0.7581658348037408 1101 72-73 -0.8013116761346524 1101 74-75 -0.9222108821146939 1101 76-77 -0.8512293636119459 1101 78-79 -0.6849733626330128 1101 80-81 -0.6249565470541967 1101 82-83 -0.7038437438660878 1101 84-85 -0.70127550535436 1101 86-87 -0.6859491656720031 1101 88-89 -0.7802047998164099 1101 90-91 -0.875500545779154 1101 92-93 -0.9489969660978197 1101 94-95 -0.9654921624971529 1101 96-97 -0.9197682606189694 1101 98-99 -1.148231356081471 1101 100 -1.0507206282516002 1102 1 -0.35133530646647415 1102 2 -0.2967359271717882 1102 3 -0.33325857822514493 1102 4 -0.23287022378789146 1102 5 -0.18775214918002092 1102 6 -0.3219705061771023 1102 7 -0.24242506886066906 1102 8 -0.3042567619074532 1102 9 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1105 70-71 -0.7503342057622149 1105 72-73 -0.7166104366335091 1105 74-75 -0.7784849121189126 1105 76-77 -0.7175069155060676 1105 78-79 -0.6265389951590912 1105 80-81 -0.6203556152051348 1105 82-83 -0.6302005077088921 1105 84-85 -0.64129917211433 1105 86-87 -0.5336164532150143 1105 88-89 -0.650245584411028 1105 90-91 -0.6820245906856996 1105 92-93 -0.7083077524515673 1105 94-95 -0.6435104549430299 1105 96-97 -0.7332140623901431 1105 98-99 -0.7860446350115886 1105 100 -0.6712127549628555 1106 1 -0.31414333486248225 1106 2 -0.2929071233718794 1106 3 -0.28288581753744424 1106 4 -0.23260013172352245 1106 5 -0.26910722683674493 1106 6 -0.369034063411128 1106 7 -0.34532027398066845 1106 8 -0.45498655511051567 1106 9 -0.5213212342304772 1106 10-11 -0.47134931004814007 1106 12-13 -0.49600233103257807 1106 14-15 -0.6323788938196273 1106 16-17 -0.5719629353592453 1106 18-19 -0.5749342094611336 1106 20-21 -0.6435818934731259 1106 22-23 -0.6319819962522715 1106 24-25 -0.6195824162563923 1106 26-27 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0.3617119675546192 2311 12-13 0.46730978861141637 2311 14-15 0.47175369022562563 2311 16-17 0.5021996383666547 2311 18-19 0.6262593253568554 2311 20-21 0.6513144136502547 2311 22-23 0.6643802040400644 2311 24-25 0.661191807749276 2311 26-27 0.71921246735387 2311 28-29 0.7855934479900384 2311 30-31 0.5907224199632921 2311 32-33 0.6263865467010348 2311 34-35 0.7677286497951457 2311 36-37 0.8276357163870216 2311 38-39 0.8956383376643444 2311 40-41 0.8322763162196196 2311 42-43 0.8312205095886753 2311 44-45 0.8675352003395389 2311 46-47 0.9492916211313371 2311 48-49 -5.689543763914948 2311 50-51 -0.8947090689899966 2311 52-53 0.48268233893158907 2311 54-55 0.9398279584518079 2311 56-57 1.0500442220721666 2311 58-59 1.0291680413256898 2311 60-61 0.8827351795247438 2311 62-63 0.7906625938831411 2311 64-65 0.9014839898581144 2311 66-67 1.0294014171843244 2311 68-69 1.038804111410819 2311 70-71 1.0269291308523805 2311 72-73 0.5144387258418277 2311 74-75 0.8592354149476407 2311 76-77 0.9455832609614525 2311 78-79 0.8883915143701131 2311 80-81 0.8441881992809144 2311 82-83 0.8492084405908003 2311 84-85 0.946965473489513 2311 86-87 0.9487217790369336 2311 88-89 0.9978347498456799 2311 90-91 1.057254074396326 2311 92-93 1.0327895155041062 2311 94-95 1.0756759375689668 2311 96-97 1.0896705244372171 2311 98-99 1.0786069768351894 2311 100 1.2778647013501931 2312 1 0.07892039701028608 2312 2 0.24701991470710993 2312 3 0.20983468688736906 2312 4 -0.01622710097677782 2312 5 0.20020645582395957 2312 6 0.11773128351848783 2312 7 0.25454112588073485 2312 8 0.3410379641172838 2312 9 0.26105515574344906 2312 10-11 0.2292822882220591 2312 12-13 0.30371555934240035 2312 14-15 0.3292207329750809 2312 16-17 0.45763625789498263 2312 18-19 0.32063563775142256 2312 20-21 0.3444636785560107 2312 22-23 0.5810221546368908 2312 24-25 0.6575809815435605 2312 26-27 0.6020462065289109 2312 28-29 0.6938924987933248 2312 30-31 0.7237066397681673 2312 32-33 0.7723155111069033 2312 34-35 0.3721825353950692 2312 36-37 0.6758664021579435 2312 38-39 0.7441330447084127 2312 40-41 0.5487419167001804 2312 42-43 0.57784128636915 2312 44-45 0.6742896002406482 2312 46-47 0.720394546433397 2312 48-49 -4.996981668525635 2312 50-51 -0.9401252305100201 2312 52-53 0.3508318586619481 2312 54-55 0.6790622132232116 2312 56-57 0.7558735971939754 2312 58-59 0.8299809279549493 2312 60-61 0.8489248988505906 2312 62-63 0.6021696066177 2312 64-65 0.7790539533879226 2312 66-67 0.863768993501985 2312 68-69 0.7653328693053041 2312 70-71 0.8016029342937898 2312 72-73 0.8936022052850703 2312 74-75 0.5869641667723258 2312 76-77 0.7825282060500065 2312 78-79 0.4015871366598276 2312 80-81 0.7047902551521048 2312 82-83 0.7232482212776432 2312 84-85 0.6972866482791886 2312 86-87 0.7949908327318091 2312 88-89 0.8343737957563349 2312 90-91 0.6118986799205643 2312 92-93 0.7826538261966753 2312 94-95 0.946325114148312 2312 96-97 0.9928951745055663 2312 98-99 1.0507424245765655 2312 100 0.7660504582876868 2313 1 0.0705888924383018 2313 2 0.22320497459328692 2313 3 0.2974162888452412 2313 4 0.07163918524573631 2313 5 0.13522152092954087 2313 6 0.242932817396742 2313 7 0.15184391337650283 2313 8 0.24428244295094004 2313 9 0.40935315375121917 2313 10-11 0.23156966893449038 2313 12-13 0.005856507831111912 2313 14-15 0.12977602628587448 2313 16-17 0.034645198909423414 2313 18-19 0.37115315210979816 2313 20-21 0.34158314447376625 2313 22-23 0.42712319681071875 2313 24-25 0.28269244200538424 2313 26-27 0.42488522405934503 2313 28-29 0.4480010451583709 2313 30-31 0.5810387034094191 2313 32-33 0.45736186663464196 2313 34-35 0.4168995096965986 2313 36-37 -0.310298429220218 2313 38-39 0.2550227167010988 2313 40-41 0.4401375411083137 2313 42-43 0.5057879474577405 2313 44-45 0.45406051547075776 2313 46-47 0.39613165698737873 2313 48-49 -5.0720114018292755 2313 50-51 -1.2283524193416682 2313 52-53 0.15426596092989087 2313 54-55 0.29852194497964746 2313 56-57 0.3598413385904067 2313 58-59 0.4116714207879255 2313 60-61 0.7037184022766212 2313 62-63 0.8372128041365698 2313 64-65 0.9095424421301814 2313 66-67 0.904275059724192 2313 68-69 0.8536448171381394 2313 70-71 0.85249799356896 2313 72-73 0.7671975253862868 2313 74-75 0.8538587160284195 2313 76-77 0.896319826849588 2313 78-79 0.830786071212259 2313 80-81 -0.11643760495919508 2313 82-83 0.3202362609852827 2313 84-85 0.7200036621298551 2313 86-87 0.8648507587527838 2313 88-89 0.8013110585950756 2313 90-91 0.8990932011395145 2313 92-93 0.8873320552182626 2313 94-95 0.8180764106892582 2313 96-97 0.7862686303866404 2313 98-99 0.9666278606146825 2313 100 1.0092108071471202 2314 1 0.0741084537451755 2314 2 0.2024967529928503 2314 3 0.21046997666354628 2314 4 0.08913532019431614 2314 5 0.1986098075395475 2314 6 0.28237686195442535 2314 7 0.09995927534692584 2314 8 0.20668432566623096 2314 9 0.40606650458965277 2314 10-11 0.27301669142417495 2314 12-13 0.1033738912751403 2314 14-15 0.2089528019604714 2314 16-17 0.40191365903559273 2314 18-19 0.4919530274883783 2314 20-21 0.5588191088500309 2314 22-23 0.3603629741287264 2314 24-25 0.389893281553384 2314 26-27 0.41891546664340495 2314 28-29 0.5749320397080382 2314 30-31 0.4881284509676931 2314 32-33 0.44757742922227095 2314 34-35 0.3154655550366243 2314 36-37 0.4114408484373655 2314 38-39 0.4484538499588737 2314 40-41 0.2564410386108591 2314 42-43 0.24297402686684677 2314 44-45 0.42642286261070694 2314 46-47 0.640330603368028 2314 48-49 -6.360426945611238 2314 50-51 -1.6026951560269964 2314 52-53 -0.9062745925410596 2314 54-55 -0.43300127434562796 2314 56-57 -0.13314138293793576 2314 58-59 0.383713178002246 2314 60-61 0.6091123202449609 2314 62-63 0.42506799202053713 2314 64-65 0.6445424820407695 2314 66-67 0.6544330704963315 2314 68-69 0.6722036203386992 2314 70-71 0.5528072014477772 2314 72-73 0.6791820864662483 2314 74-75 0.6909306022196873 2314 76-77 0.6605496130674133 2314 78-79 0.45900341024760394 2314 80-81 0.51297540748768 2314 82-83 0.5423270404455209 2314 84-85 0.47019505095191505 2314 86-87 0.540602733146951 2314 88-89 0.43870867288594084 2314 90-91 0.6833642252822543 2314 92-93 0.6996597682544312 2314 94-95 0.7567606380464547 2314 96-97 0.8246176126014824 2314 98-99 0.8810258950460188 2314 100 -0.6477659043492565 2315 1 0.18070453408058285 2315 2 0.2546514210500135 2315 3 0.3223354919830541 2315 4 0.1370743768410918 2315 5 0.2607694938274321 2315 6 0.3299865087833851 2315 7 0.3183709131147765 2315 8 0.25653139306020734 2315 9 0.41117133223884395 2315 10-11 0.26424494754655825 2315 12-13 0.4585661988923917 2315 14-15 0.30741608954851785 2315 16-17 0.4259798831911681 2315 18-19 0.5430514082283651 2315 20-21 0.4669246369829523 2315 22-23 0.4719672716583929 2315 24-25 0.5210923162147907 2315 26-27 0.5784458556748433 2315 28-29 0.6422137510622932 2315 30-31 0.6427215834950459 2315 32-33 0.7338397445244453 2315 34-35 0.7264372339885128 2315 36-37 0.5823843216735938 2315 38-39 0.6964727440903076 2315 40-41 0.7274533527421454 2315 42-43 0.7140755114357162 2315 44-45 0.7599958702082077 2315 46-47 0.634695583006291 2315 48-49 -4.901355481244089 2315 50-51 -0.8939924847826717 2315 52-53 0.37387282127963317 2315 54-55 0.34170580363316816 2315 56-57 0.5642022600965717 2315 58-59 0.7031664474727535 2315 60-61 0.6007835501222729 2315 62-63 0.3230498275474716 2315 64-65 0.587243066372416 2315 66-67 -0.2334056673957079 2315 68-69 0.566703003280729 2315 70-71 0.5678193879522837 2315 72-73 0.707948267162358 2315 74-75 0.8326024248590471 2315 76-77 0.7934248226563589 2315 78-79 0.7646733157530434 2315 80-81 0.5611134053199791 2315 82-83 0.5436395570818888 2315 84-85 0.6142253834880051 2315 86-87 0.6232687782347206 2315 88-89 0.6353815678635328 2315 90-91 0.7034173528606473 2315 92-93 0.7660769373946685 2315 94-95 0.4131046018066904 2315 96-97 0.48584772848426283 2315 98-99 0.4769284750196192 2315 100 0.4999644463941415 2316 1 0.23865237582796794 2316 2 0.24906038840695288 2316 3 0.23779218993301754 2316 4 0.009463473398668043 2316 5 0.17949301957879982 2316 6 0.15853339404709743 2316 7 0.2761675189073287 2316 8 0.29195331119379375 2316 9 0.46497666706245866 2316 10-11 0.40104804338198363 2316 12-13 0.2882579968225869 2316 14-15 0.4246120288118149 2316 16-17 0.44143764725682644 2316 18-19 0.17859053979350392 2316 20-21 0.5193804883571786 2316 22-23 0.6182777356360631 2316 24-25 0.6428228352253313 2316 26-27 0.631268470213115 2316 28-29 0.6822480233543473 2316 30-31 0.6347153873418989 2316 32-33 0.5658469194710349 2316 34-35 0.5345702906496967 2316 36-37 0.44998238380681954 2316 38-39 0.5293662774680925 2316 40-41 0.1656725467117255 2316 42-43 0.07109668928566748 2316 44-45 0.38268390875546743 2316 46-47 0.4333487109958156 2316 48-49 -5.121608142437097 2316 50-51 -1.0459840929878368 2316 52-53 0.23822035592760926 2316 54-55 0.5214453057031676 2316 56-57 0.4505583739143546 2316 58-59 0.2788821965332673 2316 60-61 0.41933506215872285 2316 62-63 0.34719615022155637 2316 64-65 0.36575380777250643 2316 66-67 0.47067329854805706 2316 68-69 0.0871103153871573 2316 70-71 -0.2580463131257673 2316 72-73 -0.11537454224153976 2316 74-75 0.0628196375233756 2316 76-77 0.1747206866574622 2316 78-79 0.21026746493910764 2316 80-81 0.13369166420273615 2316 82-83 0.04108020777598398 2316 84-85 0.18401144782858125 2316 86-87 0.3282301515679471 2316 88-89 0.1739926422475051 2316 90-91 0.21955003714371202 2316 92-93 0.28547091923081425 2316 94-95 0.4495269200785508 2316 96-97 0.5157508702389038 2316 98-99 0.5953021696057164 2316 100 -0.17339305835975338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 6.0 11 17.0 12 34.0 13 164.0 14 570.0 15 1776.0 16 4118.0 17 8173.0 18 14253.0 19 21254.0 20 31019.0 21 44512.0 22 56238.0 23 61224.0 24 65205.0 25 71650.0 26 84489.0 27 103114.0 28 127619.0 29 156588.0 30 192160.0 31 239051.0 32 300101.0 33 390268.0 34 539307.0 35 763029.0 36 1189492.0 37 1719135.0 38 1345777.0 39 176214.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02482969449432 19.923229316624617 14.527903649975734 23.52403733890533 2 15.524833538853738 20.257581535889468 39.913952631645195 24.303632293611603 3 18.71772476642323 25.36898725463621 29.919924137427213 25.99336384151335 4 13.099062805405904 16.848178628952187 37.675170767731224 32.377587797910685 5 13.777172058322426 37.4195780258269 34.29611324825752 14.507136667593153 6 31.50574600483847 36.77955646167972 17.373094029355723 14.341603504126088 7 27.922800445801265 32.23907695417925 21.634376265559283 18.20374633446021 8 26.394837143128534 35.234476418960334 19.552219515625964 18.81846692228516 9 26.30555727503757 16.078934736119205 20.41321034559772 37.2022976432455 10-11 25.3780250437942 26.183792902095632 27.36110426263544 21.07707779147473 12-13 25.73737771142166 23.687041673295216 27.91380103667444 22.66177957860868 14-15 23.20531261333681 25.19104815180304 25.481858649086853 26.121780585773298 16-17 22.54128694648398 28.14483701660613 26.28406971107183 23.029806325838067 18-19 22.269609626692645 27.2305053401175 28.518983537687053 21.9809014955028 20-21 23.26041347471841 26.279313398426318 28.417898997938835 22.042374128916443 22-23 23.27003199809928 26.05901615008996 28.16383566631586 22.507116185494898 24-25 22.866095513419943 26.31705292035505 28.383918841212335 22.432932725012673 26-27 22.764596560011963 26.46389589139393 28.146013124520092 22.625494424074013 28-29 22.511503107537276 26.477542444826963 28.446976187453078 22.56397826018268 30-31 22.942611225911424 26.300525285781195 28.295944274199424 22.460919214107953 32-33 22.90100690519726 26.573991764634126 27.950417802717176 22.574583527451438 34-35 22.976564757539617 26.53107523104714 28.19533363914875 22.29702637226449 36-37 23.002436187570545 26.309651179549963 28.03280238534957 22.655110247529922 38-39 22.92607963510005 26.359145305656856 28.215825850709546 22.49894920853355 40-41 22.833187033545745 26.144017515228146 28.43725289810158 22.585542553124537 42-43 22.77816790536049 26.18634020265713 28.141274097497348 22.894217794485026 44-45 22.78925093509992 26.53020214094181 27.972565298024456 22.70798162593382 46-47 22.83776803125549 26.43033112891319 27.69346198950394 23.03843885032738 48-49 23.181498056345234 26.44856657102943 27.57481717981709 22.795118192808243 50-51 22.949699819641303 26.548269149630233 27.444637719379415 23.05739331134905 52-53 22.787473729368386 26.72802142519381 27.355557518171814 23.128947327265994 54-55 22.764323704927882 26.70672716619657 27.33205118971378 23.19689793916177 56-57 23.00185115614531 26.5597953281828 27.187754469085434 23.250599046586455 58-59 22.682571165101688 26.603085094979807 27.497990514866817 23.216353225051694 60-61 22.78701302257216 26.79469048057625 27.274765184095685 23.14353131275591 62-63 22.793458496474695 26.676194744975213 27.186031500665198 23.344315257884897 64-65 22.533802926943732 26.558311622610574 27.307256931835553 23.600628518610137 66-67 22.72150446330413 26.455905223806397 27.212234543925785 23.61035576896369 68-69 22.526138363764357 26.49553923300209 27.3217362715141 23.656586131719454 70-71 22.55517453367146 26.278586348744675 27.335374242325027 23.830864875258833 72-73 22.686323451662503 26.530021043319568 27.156524931372513 23.62713057364542 74-75 22.616394256991793 26.659306031762593 27.09021202962889 23.634087681616723 76-77 22.53904108664596 26.621256945713384 26.987061921709664 23.852640045930997 78-79 22.49773486956456 26.476046196981606 27.069054879354393 23.95716405409944 80-81 22.389549262878848 26.738226989104007 27.05254506809987 23.81967867991727 82-83 22.59649164244799 26.44242037426322 27.31491563283614 23.64617235045265 84-85 22.689981979299652 26.488469678544423 26.954253742544942 23.86729459961098 86-87 22.22956614668437 26.73373021674917 27.200420933416474 23.836282703149987 88-89 22.388773996627112 26.31083345150669 27.172176032756678 24.128216519109525 90-91 22.428638462890728 26.333261021061823 27.163214680425074 24.074885835622375 92-93 22.4581727838658 26.432765704907712 27.035050883328665 24.074010627897824 94-95 22.01782461290985 26.22074292967922 27.328049544217386 24.433382913193547 96-97 22.103581350867017 26.195714940094543 27.290181682283738 24.4105220267547 98-99 21.956732941796055 26.34143090635421 27.155961945421325 24.545874206428415 100 22.041409768597354 26.162773264713817 27.082874963443988 24.712942003244837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1462.0 1 1026.5 2 941.0 3 1239.0 4 972.5 5 864.0 6 1393.0 7 2624.0 8 4329.5 9 5396.0 10 4919.0 11 4252.0 12 4449.0 13 4914.5 14 5552.5 15 6219.5 16 6961.5 17 7846.5 18 8591.0 19 9311.0 20 10253.0 21 11381.5 22 12841.5 23 14849.5 24 17485.0 25 20660.5 26 25105.0 27 31067.5 28 38293.0 29 48555.5 30 62336.5 31 76941.0 32 95110.5 33 115935.5 34 136451.0 35 156476.0 36 179806.0 37 207339.5 38 222371.0 39 225623.0 40 225462.0 41 225012.0 42 231376.5 43 243385.5 44 257060.0 45 276735.0 46 295639.5 47 309275.5 48 321184.5 49 326345.0 50 322398.5 51 315116.5 52 307472.0 53 298204.0 54 288403.0 55 268829.5 56 243311.5 57 213747.0 58 185673.5 59 164593.0 60 138313.5 61 110829.0 62 88255.5 63 68760.5 64 50728.0 65 35846.0 66 26210.0 67 19981.5 68 15236.0 69 11263.5 70 8432.0 71 6246.0 72 4681.5 73 3573.0 74 2628.5 75 1870.5 76 1249.5 77 719.5 78 405.0 79 260.5 80 167.0 81 108.5 82 66.0 83 35.5 84 22.0 85 13.0 86 9.5 87 9.5 88 5.5 89 1.5 90 1.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030246903487853357 2 0.001570088083239063 3 9.083154200556564E-5 4 0.021591955128180174 5 4.6713364460005183E-4 6 2.5951869144447324E-5 7 1.2975934572223662E-5 8 0.003866828502522651 9 0.009926589947751103 10-11 9.602191583445509E-4 12-13 1.038074765777893E-4 14-15 1.1678341115001296E-4 16-17 2.8547056058892057E-4 18-19 2.5951869144447324E-5 20-21 1.1029544386390113E-4 22-23 2.919585278750324E-4 24-25 3.243983643055916E-5 26-27 8.43435747194538E-5 28-29 9.018274527695445E-4 30-31 0.0025627470780141734 32-33 0.01017313270462335 34-35 0.019340630479899368 36-37 0.022156408282071903 38-39 0.021637370899182957 40-41 0.004742704086147749 42-43 0.005261741469036695 44-45 0.009251841349995472 46-47 0.011931371839159658 48-49 1.207825941851723 50-51 0.017815958167663088 52-53 0.018678857816715962 54-55 0.009459456303151051 56-57 0.0075130661173175 58-59 0.00804507943477867 60-61 0.004898415301014433 62-63 0.007500090182745277 64-65 0.019100575690313232 66-67 0.028443248582314267 68-69 0.029033653605350446 70-71 0.02595835711173344 72-73 0.02275330127239419 74-75 0.009303745088284366 76-77 0.014299479898590477 78-79 0.007993175696489777 80-81 0.0027054823583086336 82-83 0.014117816814579345 84-85 0.023661616692449847 86-87 0.023259362720710917 88-89 0.032394420659556375 90-91 0.020313825572816146 92-93 0.013040814245084782 94-95 0.0063322560712451475 96-97 0.0034256467270670473 98-99 0.015830640178112868 100 0.03429539507438714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7706574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.37242174276851 #Duplication Level Percentage of deduplicated Percentage of total 1 72.73727952795203 41.00376597971353 2 15.306014520226244 17.256742114702647 3 5.387661265794877 9.111465392477005 4 2.388753998341284 5.386393913368777 5 1.2704192540269383 3.5808304989069963 6 0.7417134626500568 2.5087310477278906 7 0.46506741181150174 1.8351883394219042 8 0.3131669999310545 1.412318575682477 9 0.24411160800958365 1.2385046267119473 >10 1.0666482319470103 10.338714754787526 >50 0.052391352405129474 2.0262226061559137 >100 0.02484009797470062 2.479863760501881 >500 0.0014254498291071194 0.5181702650901623 >1k 4.1179051918621565E-4 0.3801678133987176 >5k 0.0 0.0 >10k+ 9.502858129834042E-5 0.9229203113525865 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22203 0.28810467530708195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18777 0.2436491234626437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10705 0.1389073795956543 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.892780371667099E-5 2 0.0 0.0 0.0 0.0 3.892780371667099E-5 3 0.0 0.0 0.0 0.0 3.892780371667099E-5 4 0.0 0.0 0.0 0.0 5.190373828889465E-5 5 0.0 0.0 0.0 0.0 5.190373828889465E-5 6 0.0 0.0 0.0 0.0 7.785560743334198E-5 7 0.0 0.0 0.0 0.0 7.785560743334198E-5 8 0.0 0.0 0.0 0.0 7.785560743334198E-5 9 0.0 0.0 0.0 0.0 1.1678341115001296E-4 10-11 6.487967286111832E-5 0.0 0.0 6.487967286111831E-6 1.1678341115001296E-4 12-13 1.5571121486668396E-4 6.487967286111831E-6 0.0 1.9463901858335492E-5 1.686871494389076E-4 14-15 1.8166308401113126E-4 1.2975934572223662E-5 0.0 3.243983643055916E-5 2.0761495315557862E-4 16-17 2.2707885501391408E-4 1.2975934572223662E-5 0.0 3.892780371667099E-5 2.465427568722496E-4 18-19 2.465427568722496E-4 1.2975934572223662E-5 0.0 4.5415771002782815E-5 2.5951869144447327E-4 20-21 2.5951869144447327E-4 1.2975934572223662E-5 0.0 6.487967286111832E-5 2.919585278750324E-4 22-23 2.7898259330280873E-4 1.9463901858335492E-5 0.0 2.7898259330280873E-4 3.5683820073615074E-4 24-25 3.114224297333679E-4 2.5951869144447324E-5 0.0 9.926589947751102E-4 3.892780371667099E-4 26-27 3.2439836430559155E-4 2.5951869144447324E-5 0.0 0.0020956134334141215 4.0225397173893355E-4 28-29 3.2439836430559155E-4 2.5951869144447324E-5 0.0 0.005417452683903379 4.0874193902504534E-4 30-31 4.4118177545560453E-4 2.5951869144447324E-5 0.0 0.016771395434599084 4.2820584088338085E-4 32-33 4.541577100278282E-4 4.5415771002782815E-5 0.0 0.043067126845210336 4.801095791722755E-4 34-35 4.801095791722755E-4 5.190373828889465E-5 0.0 0.08546599305995116 5.190373828889465E-4 36-37 5.060614483167229E-4 5.190373828889465E-5 0.0 0.15301870844294754 5.190373828889465E-4 38-39 5.060614483167229E-4 5.190373828889465E-5 0.0 0.24269539227158526 5.449892520333938E-4 40-41 5.060614483167229E-4 5.190373828889465E-5 0.0 0.33651788719604847 5.449892520333938E-4 42-43 5.190373828889465E-4 5.190373828889465E-5 0.0 0.4311319141294173 5.709411211778411E-4 44-45 5.255253501750584E-4 5.190373828889465E-5 0.0 0.5506856873106001 6.098689248945122E-4 46-47 5.320133174611702E-4 5.190373828889465E-5 0.0 0.6929538339604602 6.423087613250713E-4 48-49 5.320133174611702E-4 6.487967286111832E-5 0.0 0.8441364476614381 6.747485977556304E-4 50-51 5.320133174611702E-4 7.785560743334198E-5 0.0 1.0041232329696697 6.747485977556304E-4 52-53 5.514772193195056E-4 7.785560743334198E-5 0.0 1.1618327417604761 7.26652336044525E-4 54-55 5.579651866056175E-4 7.785560743334198E-5 0.0 1.3385195548631597 7.396282706167487E-4 56-57 5.579651866056175E-4 7.785560743334198E-5 0.0 1.5441362140946158 7.526042051889724E-4 58-59 5.579651866056175E-4 8.434357471945382E-5 0.0 1.7733885381493772 7.655801397611961E-4 60-61 5.579651866056175E-4 9.731950929167747E-5 0.0 2.009725722480573 7.655801397611961E-4 62-63 5.579651866056175E-4 1.038074765777893E-4 0.0 2.245608749101741 7.655801397611961E-4 64-65 5.579651866056175E-4 1.038074765777893E-4 0.0 2.489043769643943 7.72068107047308E-4 66-67 5.579651866056175E-4 1.038074765777893E-4 0.0 2.7643152456590956 7.980199761917553E-4 68-69 5.579651866056175E-4 1.038074765777893E-4 0.0 3.074524425509961 8.174838780500908E-4 70-71 5.579651866056175E-4 1.038074765777893E-4 0.0 3.404820352078628 8.564116817667617E-4 72-73 5.579651866056175E-4 1.038074765777893E-4 0.0 3.732735194653292 9.083154200556564E-4 74-75 5.579651866056175E-4 1.038074765777893E-4 0.0 4.055732158025084 9.083154200556564E-4 76-77 5.709411211778411E-4 1.038074765777893E-4 0.0 4.411499844159025 9.083154200556564E-4 78-79 5.968929903222885E-4 1.038074765777893E-4 0.0 4.799642487050667 9.083154200556564E-4 80-81 5.968929903222885E-4 1.038074765777893E-4 0.0 5.213217961703865 9.277793219139919E-4 82-83 5.968929903222885E-4 1.038074765777893E-4 0.0 5.6275200886931085 9.99146962061222E-4 84-85 6.033809576084003E-4 1.038074765777893E-4 0.0 6.03668374559175 0.0010445627330640049 86-87 6.098689248945122E-4 1.1678341115001296E-4 0.0 6.462106248509389 0.001122418340497347 88 6.098689248945122E-4 1.2975934572223663E-4 0.0 6.797534157201371 0.0011289063077834587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13815 0.0 47.65846 1 GTATCAA 23915 0.0 40.128796 1 CAACGCA 29040 0.0 32.321747 5 ATCAACG 29390 0.0 31.943674 3 TATCAAC 30355 0.0 31.420937 2 TCAACGC 30430 0.0 30.883179 4 AACGCAG 31050 0.0 30.486244 6 ACGCAGA 35495 0.0 26.3381 7 GTGGTAT 6355 0.0 26.288612 1 CGCAGAG 36570 0.0 25.474089 8 GCAGAGT 40055 0.0 22.975899 9 TGGTATC 7070 0.0 22.6944 2 GTACATG 32490 0.0 22.590208 1 TACCTGG 5420 0.0 22.15913 2 TACATGG 33180 0.0 21.902164 2 GAGTACT 20620 0.0 21.540041 12-13 ACATGGG 33590 0.0 20.8958 3 GTACTTT 22220 0.0 19.894016 14-15 CAGAGTA 39075 0.0 19.709211 10-11 AGAGTAC 37460 0.0 19.394506 10-11 >>END_MODULE