FastQCFastQC Report
Mon 6 Jun 2016
ERR1211308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10536903
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230330.2185936417939882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160340.15216994974709364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132200.043.340871
GTATCAA242900.043.1682431
ATCAACG292800.034.747453
CAACGCA293200.034.699725
TCAACGC301550.033.7854654
TATCAAC308600.033.458622
AACGCAG316250.032.586376
TACCTGG89950.029.5472262
ACGCAGA359450.028.4218947
CGCAGAG372800.027.3015758
GTACATG354500.026.022151
GTACCTG108900.025.2702641
TACATGG363300.024.7437042
GCAGAGT414750.023.782539
ACATGGG376300.023.2281633
ACCTGGG111050.022.851323
CATGGGG273050.021.6333124
GTGGTAT72150.020.34231
AGAGTAC376300.020.03812610-11
GTACTAG32250.019.8376871
Produced by FastQC (version 0.11.5)