FastQCFastQC Report
Mon 6 Jun 2016
ERR1211308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10536903
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211040.20028655478749308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158130.15007255927097363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132900.044.775091
GTATCAA246550.041.9135551
ATCAACG303600.033.5212023
CAACGCA304850.033.4846575
TCAACGC314250.032.4601254
TATCAAC319700.032.1544382
AACGCAG328550.031.4544496
TACCTGG91900.027.4667912
ACGCAGA376550.027.270447
CGCAGAG392300.026.0799278
GTACATG360200.024.5328981
TACATGG366550.023.769492
GCAGAGT438800.023.1118899
GTACCTG113800.023.0521681
ACATGGG376000.022.1998423
ACCTGGG110600.021.9329573
CATGGGG273100.020.445194
AGAGTAC400150.020.27248610-11
GTGGTAT74200.019.353511
GAGTACT233950.018.87522312-13
Produced by FastQC (version 0.11.5)