##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10536903 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.105674599073375 33.0 31.0 34.0 30.0 34.0 2 32.23583362208041 34.0 31.0 34.0 30.0 34.0 3 32.30644250972036 34.0 31.0 34.0 30.0 34.0 4 35.945412897888495 37.0 35.0 37.0 35.0 37.0 5 35.845440448678325 37.0 35.0 37.0 35.0 37.0 6 35.85539650502619 37.0 35.0 37.0 35.0 37.0 7 35.769746195822435 37.0 35.0 37.0 33.0 37.0 8 35.744866779166514 37.0 35.0 37.0 33.0 37.0 9 37.36355834347151 39.0 37.0 39.0 34.0 39.0 10-11 37.30964183688509 39.0 37.0 39.0 34.0 39.0 12-13 37.24591651835459 39.0 37.0 39.0 33.0 39.0 14-15 38.49462731126974 40.0 38.0 41.0 34.0 41.0 16-17 38.476825496068436 40.0 38.0 41.0 34.0 41.0 18-19 38.4653636841869 40.0 38.0 41.0 34.0 41.0 20-21 38.42501895481054 40.0 38.0 41.0 34.0 41.0 22-23 38.31967514553375 40.0 38.0 41.0 34.0 41.0 24-25 38.23482032623818 40.0 38.0 41.0 33.0 41.0 26-27 38.144901400345056 40.0 38.0 41.0 33.0 41.0 28-29 38.03972822944275 40.0 37.5 41.0 33.0 41.0 30-31 38.05874320946107 40.0 37.0 41.0 33.0 41.0 32-33 38.014080750292564 40.0 37.0 41.0 33.0 41.0 34-35 37.89528782793198 40.0 37.0 41.0 33.0 41.0 36-37 37.75400850705373 40.0 37.0 41.0 33.0 41.0 38-39 37.64596708349693 40.0 37.0 41.0 32.5 41.0 40-41 37.54216699157238 40.0 37.0 41.0 32.0 41.0 42-43 37.44489253626042 40.0 36.5 41.0 32.0 41.0 44-45 37.30837799304027 40.0 36.0 41.0 31.0 41.0 46-47 37.095586055978686 39.0 36.0 41.0 31.0 41.0 48-49 35.55457163267043 39.0 35.0 41.0 25.0 41.0 50-51 36.31133564577752 39.0 35.0 40.0 29.0 41.0 52-53 36.41209380972758 39.0 35.0 40.0 30.0 41.0 54-55 36.246594089363825 38.5 35.0 40.0 29.0 41.0 56-57 36.03690738160918 38.0 35.0 40.0 29.0 41.0 58-59 35.80522137292144 38.0 34.5 40.0 28.0 41.0 60-61 35.54903936194535 38.0 34.0 40.0 28.0 41.0 62-63 35.227272235494624 37.0 34.0 40.0 28.0 41.0 64-65 34.8893207994797 36.5 34.0 39.5 27.5 41.0 66-67 34.55032920963588 36.0 33.0 39.0 27.0 40.5 68-69 34.18603583045227 35.5 33.0 39.0 26.0 40.0 70-71 33.81671108673962 35.0 33.0 38.0 26.0 40.0 72-73 33.43506521792978 35.0 32.0 37.5 26.0 39.5 74-75 33.06868778235882 35.0 32.0 37.0 26.0 39.0 76-77 32.130875694689415 34.0 30.5 36.0 24.5 38.0 78-79 32.5647569309502 34.5 32.0 36.0 26.0 38.0 80-81 32.43717347497647 35.0 32.0 35.5 25.5 37.0 82-83 32.16706360493211 34.0 31.5 35.0 25.0 37.0 84-85 31.887670361964993 34.0 31.0 35.0 25.0 36.5 86-87 31.623975801997986 34.0 31.0 35.0 24.0 36.0 88-89 31.397380662989875 34.0 31.0 35.0 24.0 36.0 90-91 31.18221103487429 34.0 31.0 35.0 23.0 35.0 92-93 30.994181212449234 34.0 30.0 35.0 23.0 35.0 94-95 30.79731658344012 34.0 30.0 35.0 22.0 35.0 96-97 30.603126127288064 34.0 30.0 35.0 20.0 35.0 98-99 30.382533795746244 34.0 29.0 35.0 20.0 35.0 100 27.94421681588983 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31461568918092553 1101 2 -0.3098167328359622 1101 3 -0.3507764512919316 1101 4 -0.20232715542554303 1101 5 -0.21391771033269436 1101 6 -0.2543868335172874 1101 7 -0.2904613190459102 1101 8 -0.2150575248837967 1101 9 -0.357422979221667 1101 10-11 -0.32793557528532347 1101 12-13 -0.3940715456215145 1101 14-15 -0.5254453867548889 1101 16-17 -0.5137170937946891 1101 18-19 -0.5634285173560301 1101 20-21 -0.5863347264226775 1101 22-23 -0.6894480435542221 1101 24-25 -0.5759420148956806 1101 26-27 -0.7393586201157731 1101 28-29 -0.7045275022636588 1101 30-31 -0.6296715623078271 1101 32-33 -0.5343669999165286 1101 34-35 -0.6577243403802484 1101 36-37 -0.6568279245046611 1101 38-39 -0.6583713792883046 1101 40-41 -0.6440100542430045 1101 42-43 -0.7803326848806762 1101 44-45 -0.7364470707126998 1101 46-47 -0.7757147160718532 1101 48-49 0.5114088227155591 1101 50-51 -0.48102144424071724 1101 52-53 -0.6733188058512525 1101 54-55 -0.7074294993739443 1101 56-57 -0.7078637138995347 1101 58-59 -0.8496839114904375 1101 60-61 -0.8869048692955843 1101 62-63 -0.8620032397829291 1101 64-65 -0.8975255780697395 1101 66-67 -0.9337928209289075 1101 68-69 -0.9067391291996714 1101 70-71 -0.8413361551178724 1101 72-73 -0.9357201521455636 1101 74-75 -0.9568282639535965 1101 76-77 -0.9644730657840839 1101 78-79 -0.8127800230244695 1101 80-81 -0.7413126031517763 1101 82-83 -0.7839469800197563 1101 84-85 -0.799708939372124 1101 86-87 -0.7246642510789663 1101 88-89 -0.7107175246581185 1101 90-91 -0.7904034447216155 1101 92-93 -0.7972540599075337 1101 94-95 -0.793967636628075 1101 96-97 -0.961905691470605 1101 98-99 -1.111877128575987 1101 100 -1.0006145309025278 1102 1 -0.2966051528853768 1102 2 -0.30088763980946354 1102 3 -0.30678036902922656 1102 4 -0.20222725305470135 1102 5 -0.16494590104046836 1102 6 -0.24947991958532612 1102 7 -0.269263812151884 1102 8 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1105 68-69 -0.9236380574354044 1105 70-71 -0.9636444174127803 1105 72-73 -0.8080004797724527 1105 74-75 -0.8539986777513704 1105 76-77 -0.8487370412639699 1105 78-79 -0.7425093428912106 1105 80-81 -0.7091608521546107 1105 82-83 -0.6864060079243899 1105 84-85 -0.6387834423112757 1105 86-87 -0.6444563394370029 1105 88-89 -0.6552838639854279 1105 90-91 -0.7220169072943534 1105 92-93 -0.6657166218182802 1105 94-95 -0.7654609310033038 1105 96-97 -0.8513021355608288 1105 98-99 -0.8246837903101643 1105 100 -0.935618493378982 1106 1 -0.3197312896918838 1106 2 -0.3130715802160182 1106 3 -0.3602828402799254 1106 4 -0.20290443696662663 1106 5 -0.2755632040711049 1106 6 -0.3432538376519716 1106 7 -0.3536798663280365 1106 8 -0.35939794391611457 1106 9 -0.4449616711018294 1106 10-11 -0.487031648661592 1106 12-13 -0.46571805382114206 1106 14-15 -0.5978432113843368 1106 16-17 -0.5610837434057743 1106 18-19 -0.6455771193188937 1106 20-21 -0.6533573688070788 1106 22-23 -0.6197806759076343 1106 24-25 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0.36808139937176065 2311 7 0.39621027355017446 2311 8 0.19462759852772393 2311 9 0.47806279667139506 2311 10-11 0.40092946644140426 2311 12-13 0.4854932591921397 2311 14-15 0.4395979612100547 2311 16-17 0.45788767896281257 2311 18-19 0.6214757594680123 2311 20-21 0.6941639630562122 2311 22-23 0.6832361688972526 2311 24-25 0.6320398508604299 2311 26-27 0.7085004597996161 2311 28-29 0.7936120297762557 2311 30-31 0.7047814065816027 2311 32-33 0.6741847060675497 2311 34-35 0.7489763568043699 2311 36-37 0.8421032473877474 2311 38-39 0.8449360960350774 2311 40-41 0.772177058916725 2311 42-43 0.8190076953089473 2311 44-45 0.7985044965667427 2311 46-47 0.8729848619139275 2311 48-49 -6.054647478087208 2311 50-51 -0.9930004288076262 2311 52-53 0.440748960669886 2311 54-55 0.862448135648016 2311 56-57 0.9701978321606575 2311 58-59 1.0394598732412064 2311 60-61 0.8984194627101374 2311 62-63 0.7647472619812916 2311 64-65 0.8609425810569249 2311 66-67 0.9646709420455082 2311 68-69 0.9717452430334603 2311 70-71 1.0090769035113851 2311 72-73 0.5004040916875496 2311 74-75 0.806251634821173 2311 76-77 0.9139913194824132 2311 78-79 0.8516943474720904 2311 80-81 0.8529716092497921 2311 82-83 0.8459512127449074 2311 84-85 0.8911584411461 2311 86-87 0.9339047528782807 2311 88-89 0.9960883632411033 2311 90-91 1.027305588824003 2311 92-93 1.0563925155839833 2311 94-95 1.0584416186218064 2311 96-97 1.1040890084760484 2311 98-99 1.1441012561234487 2311 100 1.396692086973303 2312 1 0.04671351687497349 2312 2 0.22285661441480897 2312 3 0.2247765697835078 2312 4 0.021306916086224703 2312 5 0.19599654715698733 2312 6 0.115460703383647 2312 7 0.29165359893604403 2312 8 0.3315772201212184 2312 9 0.2536939752248273 2312 10-11 0.20244231356911513 2312 12-13 0.26762507124714574 2312 14-15 0.35214800182193784 2312 16-17 0.5092152446441958 2312 18-19 0.36532744630942915 2312 20-21 0.39438522400310205 2312 22-23 0.6365836425979268 2312 24-25 0.7213778389818586 2312 26-27 0.6282250150640252 2312 28-29 0.7924546459027155 2312 30-31 0.8220624030648196 2312 32-33 0.8146351617623395 2312 34-35 0.4155700806020519 2312 36-37 0.7531817223269854 2312 38-39 0.8146056366890448 2312 40-41 0.5780640731729676 2312 42-43 0.6308595313703265 2312 44-45 0.7743280486138957 2312 46-47 0.8432037971065469 2312 48-49 -4.647011734386972 2312 50-51 -0.698958103906044 2312 52-53 0.6215006111082033 2312 54-55 0.8759818664639667 2312 56-57 0.9567721628298926 2312 58-59 0.9975583627854334 2312 60-61 1.0250787148393883 2312 62-63 0.8422836459608547 2312 64-65 1.0675625221997649 2312 66-67 0.9939683948116524 2312 68-69 0.9180089502256479 2312 70-71 0.9511673029289298 2312 72-73 1.099615384959968 2312 74-75 0.7231210055939599 2312 76-77 0.9045304599317419 2312 78-79 0.5588146400743881 2312 80-81 0.7960914213966248 2312 82-83 0.7654995326812397 2312 84-85 0.8302977289569604 2312 86-87 0.9813152127053151 2312 88-89 1.0023501491580156 2312 90-91 0.8099863581430746 2312 92-93 1.0075861932491996 2312 94-95 1.073951120052243 2312 96-97 1.142883436941755 2312 98-99 1.1540207620687433 2312 100 0.7267037458072885 2313 1 0.0891079622968931 2313 2 0.2074041119755563 2313 3 0.32494438543421467 2313 4 0.03440986612275054 2313 5 0.14056913042500696 2313 6 0.24049309353704018 2313 7 0.1498433759105069 2313 8 0.21607006762890535 2313 9 0.4331324925822173 2313 10-11 0.23433388519800502 2313 12-13 -0.018477553554916426 2313 14-15 0.06155120991333973 2313 16-17 0.06646344119243963 2313 18-19 0.37107309963082713 2313 20-21 0.3740531382904919 2313 22-23 0.48318054380662545 2313 24-25 0.2934439308899286 2313 26-27 0.45644936819400783 2313 28-29 0.41375750316744586 2313 30-31 0.5600271951013767 2313 32-33 0.5145189970764079 2313 34-35 0.416639088956849 2313 36-37 -0.32360313507636107 2313 38-39 0.2083448732944433 2313 40-41 0.4045250290423823 2313 42-43 0.531916809957643 2313 44-45 0.5291227243657985 2313 46-47 0.3806186229994779 2313 48-49 -5.480778802531585 2313 50-51 -1.3638238513854688 2313 52-53 0.0552625474795434 2313 54-55 0.3535532181063914 2313 56-57 0.37219774012105944 2313 58-59 0.4368506845862683 2313 60-61 0.8201324185134737 2313 62-63 0.8389711862819311 2313 64-65 0.9141204352608625 2313 66-67 0.9621152781591178 2313 68-69 0.9550535553323627 2313 70-71 0.8910625747662948 2313 72-73 0.6064659690490757 2313 74-75 0.7699373067717374 2313 76-77 0.7748366201923531 2313 78-79 0.6840055820280355 2313 80-81 -0.2298854816248621 2313 82-83 0.22416643127740343 2313 84-85 0.5836021244207465 2313 86-87 0.7392192098901766 2313 88-89 0.799931484432097 2313 90-91 0.809835404033123 2313 92-93 0.8523685794038052 2313 94-95 0.7679298838413828 2313 96-97 0.8179700772697593 2313 98-99 1.0371035715613246 2313 100 1.1234459361814118 2314 1 0.11520100476265327 2314 2 0.2334784227644633 2314 3 0.24745915365853222 2314 4 0.1343228686179927 2314 5 0.2353825059425958 2314 6 0.2801722998169467 2314 7 0.1523628126450589 2314 8 0.2544968320775496 2314 9 0.4408879962566985 2314 10-11 0.30231060342655525 2314 12-13 0.23891802029393716 2314 14-15 0.2990260449534148 2314 16-17 0.49025861777255386 2314 18-19 0.531687070291774 2314 20-21 0.5939886987023115 2314 22-23 0.3997770551038542 2314 24-25 0.4759417233683578 2314 26-27 0.5361115875938154 2314 28-29 0.6435470422987706 2314 30-31 0.5341914872332723 2314 32-33 0.5352055765634915 2314 34-35 0.4665481977521253 2314 36-37 0.5604119919971069 2314 38-39 0.541028567841316 2314 40-41 0.3356553239667406 2314 42-43 0.34347655018726897 2314 44-45 0.5638665202182906 2314 46-47 0.6799804098809332 2314 48-49 -6.552694808775655 2314 50-51 -1.4700312048916473 2314 52-53 -0.7043192029943484 2314 54-55 -0.34476126172869215 2314 56-57 -0.037073516435192744 2314 58-59 0.4586511766399113 2314 60-61 0.6996314823881065 2314 62-63 0.543708604194947 2314 64-65 0.6768136318765343 2314 66-67 0.7682142400967464 2314 68-69 0.7897050878520631 2314 70-71 0.5802373520525492 2314 72-73 0.7812522314468566 2314 74-75 0.8501902747928298 2314 76-77 0.7713555902068734 2314 78-79 0.5596555679399771 2314 80-81 0.5884638135859745 2314 82-83 0.6356007282179661 2314 84-85 0.6388874078591691 2314 86-87 0.6743304708110394 2314 88-89 0.5860243834629877 2314 90-91 0.8223729017718959 2314 92-93 0.8317229574486049 2314 94-95 0.9345336913232316 2314 96-97 0.9814550507316895 2314 98-99 1.061395717212033 2314 100 -0.3599576343905717 2315 1 0.1813768910601894 2315 2 0.24475073307756645 2315 3 0.3029964518192685 2315 4 0.1402255380316575 2315 5 0.21637834157899505 2315 6 0.31280238761151935 2315 7 0.34482069923703307 2315 8 0.2797572313446963 2315 9 0.47013264049444103 2315 10-11 0.24390310203796872 2315 12-13 0.44019877927192397 2315 14-15 0.28026815777332104 2315 16-17 0.43361924271344066 2315 18-19 0.49748923115617316 2315 20-21 0.3697466513467873 2315 22-23 0.489290423383018 2315 24-25 0.5438421573518681 2315 26-27 0.577188903723993 2315 28-29 0.6723407825544356 2315 30-31 0.6468412201260705 2315 32-33 0.6791136919578094 2315 34-35 0.7038649951113385 2315 36-37 0.641256729292607 2315 38-39 0.6855196632878986 2315 40-41 0.7461302897232756 2315 42-43 0.766175122977053 2315 44-45 0.777995005678207 2315 46-47 0.6626919810616911 2315 48-49 -4.867009438675829 2315 50-51 -0.8214317424708497 2315 52-53 0.32705078711271796 2315 54-55 0.22577378085159694 2315 56-57 0.599959296644343 2315 58-59 0.7500233090422412 2315 60-61 0.6102386591929232 2315 62-63 0.4058651319334743 2315 64-65 0.6047287611185439 2315 66-67 -0.10951313942975816 2315 68-69 0.6117490168601378 2315 70-71 0.6047793689512275 2315 72-73 0.7271401766781409 2315 74-75 0.8131557552044697 2315 76-77 0.7884044136172363 2315 78-79 0.751904402379985 2315 80-81 0.6212830249802863 2315 82-83 0.5894778921699881 2315 84-85 0.5851743001455922 2315 86-87 0.6674288092017129 2315 88-89 0.6697754306279045 2315 90-91 0.7112127486180313 2315 92-93 0.7149220695241034 2315 94-95 0.36546267905594476 2315 96-97 0.4736735343819376 2315 98-99 0.502147157645279 2315 100 0.6295165805984944 2316 1 0.2723033431973718 2316 2 0.2961537926840023 2316 3 0.24393603401789932 2316 4 0.03685384391334168 2316 5 0.17802492119719915 2316 6 0.1388358591608565 2316 7 0.2531734208040959 2316 8 0.3121489932774608 2316 9 0.4654564025650103 2316 10-11 0.4617956665953713 2316 12-13 0.3368657398267345 2316 14-15 0.5020988518235256 2316 16-17 0.4551663805830728 2316 18-19 0.17542069334265165 2316 20-21 0.5060790514192064 2316 22-23 0.6051633627297264 2316 24-25 0.6283850871166905 2316 26-27 0.6396835862942822 2316 28-29 0.6739832882313976 2316 30-31 0.623273036639155 2316 32-33 0.5985920155249858 2316 34-35 0.5371487375897885 2316 36-37 0.46431574288444466 2316 38-39 0.6314955675609681 2316 40-41 0.13938210247370364 2316 42-43 0.11925720125145034 2316 44-45 0.4026004434565422 2316 46-47 0.4072439862439907 2316 48-49 -5.126889908771574 2316 50-51 -1.0648995883090961 2316 52-53 0.23316443223131245 2316 54-55 0.526124868033655 2316 56-57 0.492704239356236 2316 58-59 0.3810584972589268 2316 60-61 0.5074154856104158 2316 62-63 0.43913207312139235 2316 64-65 0.44734550818053975 2316 66-67 0.5777777393106405 2316 68-69 0.23537708519541667 2316 70-71 -0.24744805794868796 2316 72-73 -0.08211135224404131 2316 74-75 0.16578282884388784 2316 76-77 0.19303117457739205 2316 78-79 0.2978863995330343 2316 80-81 0.23496954099084633 2316 82-83 0.1341486199341304 2316 84-85 0.3126873671745152 2316 86-87 0.36643581212259235 2316 88-89 0.18497915283977662 2316 90-91 0.25681438049754846 2316 92-93 0.3258042442026685 2316 94-95 0.5562108780736992 2316 96-97 0.6495170305030413 2316 98-99 0.5837533698773747 2316 100 -0.1096462785717982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 11.0 11 16.0 12 57.0 13 213.0 14 795.0 15 2163.0 16 5233.0 17 10305.0 18 17802.0 19 26207.0 20 37218.0 21 51692.0 22 65506.0 23 74168.0 24 80736.0 25 93780.0 26 112578.0 27 139633.0 28 173472.0 29 213628.0 30 263793.0 31 326498.0 32 410215.0 33 533858.0 34 731175.0 35 1034860.0 36 1607337.0 37 2382052.0 38 1906372.0 39 235505.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23986808166861 19.570164204906305 14.121318158041882 24.0686495553832 2 15.550849882567498 20.4501109503923 39.90408373938269 24.094955427657506 3 18.737257867451262 25.45704645031069 29.76527997719252 26.04041570504553 4 13.07546129028859 16.79331212497723 37.72573747259844 32.40548911213574 5 13.867909871744413 37.1467528581357 34.307061948584064 14.678275321535816 6 31.421559606863465 36.66540791555466 17.87841944769519 14.034613029886685 7 28.08600934942826 31.8203710939298 21.920097759293743 18.173521797348197 8 26.133524795306208 35.31857443716919 19.984425523918354 18.563475243606256 9 26.393262986049976 16.321512553596897 20.29253523098571 36.99268922936742 10-11 25.24831700300191 26.081265691754986 27.833721742503297 20.836695562739806 12-13 25.671228559200788 23.449923537696105 28.151789358370067 22.727058544733044 14-15 23.0391743748249 25.047869921365777 25.63719003181964 26.27576567198968 16-17 22.766947708460208 27.929440506366838 26.32130594416727 22.98230584100568 18-19 22.60119460171449 27.168865874321114 28.08766543407782 22.14227408988658 20-21 23.428146039016326 26.35291615515062 27.939607264814644 22.27933054101841 22-23 23.28990962211738 26.139683796041897 27.760724959119738 22.80968162272098 24-25 22.976105208942403 26.30567114670075 27.92668412215017 22.791539522206676 26-27 22.92744496799649 26.502844790610208 27.76391405377952 22.805796187613776 28-29 22.80005339384256 26.58771824887367 27.868264122616786 22.743964234666983 30-31 23.22362787506148 26.348784916608714 27.799871353462645 22.627715854867155 32-33 23.102404518353627 26.531389472898926 27.580576379384986 22.785629629362464 34-35 23.192963388356794 26.519777121757205 27.754444750306124 22.532814739579873 36-37 23.20971310747324 26.428680491062323 27.589755633097994 22.771850768366438 38-39 23.13639853198904 26.471583688608913 27.681100367477363 22.710917411924683 40-41 23.17488588269076 26.205323223507726 27.83223783911731 22.7875530546842 42-43 23.007746929124583 26.318090526161836 27.655235292881038 23.018927251832547 44-45 22.92954561934957 26.544738743369827 27.612556810394608 22.913158826885997 46-47 22.98897405318714 26.388408838810445 27.365064428639585 23.25755267936283 48-49 23.255705589456003 26.454106088970846 27.2910807172542 22.99910760431895 50-51 23.05046070764763 26.53951038771849 27.247114353892538 23.16291455074134 52-53 22.978853367511817 26.65556862587824 27.134358513862388 23.231219492747552 54-55 22.90904538498011 26.541956574942443 27.24107740263256 23.30792063744489 56-57 23.040040326153235 26.57979257106799 27.051091673899347 23.32907542887943 58-59 22.885580261166854 26.5580712950228 27.290439665896706 23.26590877791364 60-61 23.073704922725348 26.662665608365078 27.12346433248083 23.14016513642875 62-63 22.977278887907712 26.637132663079257 27.051882745272827 23.333705703740208 64-65 22.76380604474182 26.469566415898786 27.146382451677965 23.62024508768143 66-67 22.831213672596135 26.4383495776287 27.073960627980377 23.65647612179479 68-69 22.68465870389626 26.541881345246637 27.150433591161228 23.623026359695878 70-71 22.71926517420088 26.30546060060156 27.196610318965895 23.77866390623166 72-73 22.75758330282226 26.542103334144322 26.949562610287202 23.75075075274621 74-75 22.779586514599895 26.644961824801268 26.8781977577991 23.69725390279974 76-77 22.715556110934536 26.574090074001823 26.8255625335982 23.88479128146544 78-79 22.631724038698316 26.47623790535196 26.95748728871693 23.934550767232786 80-81 22.60739372949912 26.76284135745809 26.77841091005672 23.85135400298607 82-83 22.81038065108297 26.535451531365297 26.975478791917133 23.6786890256346 84-85 22.864944421970314 26.597748210648543 26.664800388178335 23.87250697920281 86-87 22.548847622983008 26.711027816088617 26.881017819356938 23.859106741571434 88-89 22.555046636272014 26.455014851759618 26.902632494328532 24.087306017639833 90-91 22.66793504664301 26.451218630092672 26.890913246872383 23.98993307639194 92-93 22.662376655495052 26.511958660835766 26.7908590604046 24.034805623264585 94-95 22.417709337909574 26.345976745415477 26.927350849583796 24.308963067091145 96-97 22.439564209816503 26.31671308075244 26.925174577782883 24.318548131648175 98-99 22.294977960421733 26.374823610048608 26.84006641891748 24.490132010612182 100 22.280219621121002 26.298350430176647 26.8592514490568 24.56217849964555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1030.0 1 770.5 2 1106.5 3 1524.0 4 1044.0 5 826.0 6 1200.5 7 2176.5 8 3545.5 9 4363.0 10 4290.0 11 4052.5 12 4326.5 13 4931.5 14 5611.0 15 6319.5 16 7123.5 17 7945.5 18 8706.5 19 9608.0 20 10570.0 21 11920.0 22 13926.5 23 16595.0 24 20073.0 25 24812.5 26 31492.5 27 39993.0 28 50571.0 29 64865.0 30 82900.5 31 101908.5 32 125595.0 33 152699.5 34 179075.5 35 205595.5 36 235460.0 37 267140.0 38 288959.5 39 302496.5 40 311695.5 41 320788.5 42 334223.0 43 351533.5 44 371603.0 45 396109.0 46 417101.5 47 430459.0 48 443008.5 49 450382.5 50 447530.0 51 439751.5 52 427833.5 53 413620.5 54 397941.5 55 368912.5 56 331990.5 57 292071.5 58 254319.5 59 220911.0 60 183626.0 61 148052.5 62 119103.0 63 93169.5 64 69574.0 65 50735.0 66 37040.5 67 28157.5 68 21174.5 69 15327.5 70 11498.5 71 8533.5 72 6520.5 73 5090.5 74 3818.5 75 2642.0 76 1752.0 77 1037.0 78 630.5 79 387.5 80 217.5 81 126.5 82 91.0 83 69.0 84 37.5 85 23.0 86 15.0 87 8.0 88 8.0 89 6.5 90 2.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02954378530389812 2 0.0020119763843322843 3 8.541409178769132E-5 4 0.022549320231950505 5 4.935036414399943E-4 6 3.796181857230725E-5 7 1.8980909286153626E-5 8 0.0041093668604522595 9 0.010230710105236804 10-11 0.0011341093298476792 12-13 1.0914022839538334E-4 14-15 1.423568196461522E-4 16-17 3.4165636715076527E-4 18-19 1.8980909286153626E-5 20-21 6.168795517999929E-5 22-23 3.1318500322153486E-4 24-25 3.796181857230725E-5 26-27 1.0914022839538334E-4 28-29 0.0010202238741307576 30-31 0.0026620725273830462 32-33 0.009775168282369118 34-35 0.019009380650082855 36-37 0.021956166816758205 38-39 0.0212633636278136 40-41 0.004617106183856869 42-43 0.005072648006724557 44-45 0.0092911550955722 46-47 0.01190577534973986 48-49 1.2186075927623135 50-51 0.01816473018684902 52-53 0.01908055905990593 54-55 0.009751442145761425 56-57 0.00771099439749991 58-59 0.008351600085907596 60-61 0.00497299823297225 62-63 0.007582873259818373 64-65 0.01955982701938131 66-67 0.029444135530145812 68-69 0.029724103942116577 70-71 0.02671088459293969 72-73 0.023498365696258187 74-75 0.009537906916292197 76-77 0.014752911742662906 78-79 0.008441759405016826 80-81 0.0029657670759615042 82-83 0.014548866967836754 84-85 0.02438572320538587 86-87 0.02334651842196896 88-89 0.032941368066119615 90-91 0.020926452487984373 92-93 0.01349068127513369 94-95 0.007060898254449149 96-97 0.003895831630983032 98-99 0.015673485843041356 100 0.03484894944937805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0536903E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.30686760934031 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64603728095032 43.52376613302251 2 14.596355769081143 17.021355668132895 3 4.891848410438 8.556850728971108 4 2.1377572815740784 4.9858372319057205 5 1.1145173019079615 3.249200638533333 6 0.6365769113913434 2.227008341739469 7 0.41598229614408183 1.6978237268371659 8 0.294820114466531 1.3752029906216463 9 0.2093431045501768 1.0985526613741903 >10 0.9765600269238955 9.918546740793335 >50 0.05233147195249659 2.0774244138528015 >100 0.026037713235470987 2.6999973290677763 >500 0.0013155673361313406 0.507909520057269 >1k 4.462841366323732E-4 0.41587722533529337 >5k 0.0 0.0 >10k+ 7.04659117714023E-5 0.6446466497555149 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21104 0.20028655478749308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15813 0.15007255927097363 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.847136392923044E-5 2 0.0 0.0 0.0 0.0 2.847136392923044E-5 3 0.0 0.0 0.0 0.0 2.847136392923044E-5 4 0.0 0.0 0.0 0.0 3.796181857230725E-5 5 0.0 0.0 0.0 0.0 3.796181857230725E-5 6 0.0 0.0 0.0 0.0 3.796181857230725E-5 7 0.0 0.0 0.0 0.0 3.796181857230725E-5 8 0.0 0.0 0.0 0.0 3.796181857230725E-5 9 0.0 0.0 0.0 0.0 7.59236371446145E-5 10-11 0.0 0.0 0.0 9.490454643076813E-6 9.490454643076813E-5 12-13 0.0 0.0 0.0 9.490454643076813E-6 1.423568196461522E-4 14-15 0.0 0.0 0.0 9.490454643076813E-6 1.7557341089692103E-4 16-17 0.0 0.0 0.0 9.490454643076813E-6 2.2777091143384351E-4 18-19 0.0 0.0 0.0 2.3726136607692033E-5 2.4675182071999714E-4 20-21 0.0 9.490454643076813E-6 0.0 9.964977375230654E-5 2.56242275363074E-4 22-23 0.0 9.490454643076813E-6 0.0 3.701277310799957E-4 3.03694548578458E-4 24-25 0.0 9.490454643076813E-6 0.0 0.0010202238741307574 3.796181857230725E-4 26-27 0.0 9.490454643076813E-6 0.0 0.0022966900236245885 4.507965955461486E-4 28-29 0.0 9.490454643076813E-6 0.0 0.005585132557450704 4.5554182286768703E-4 30-31 0.0 9.490454643076813E-6 0.0 0.01670794539913673 4.650322775107638E-4 32-33 0.0 9.490454643076813E-6 0.0 0.04392182408815949 4.8401318679691744E-4 34-35 0.0 9.490454643076813E-6 0.0 0.08406170200105287 5.029940960830711E-4 36-37 0.0 9.490454643076813E-6 0.0 0.14493822330906908 5.172297780476863E-4 38-39 0.0 9.490454643076813E-6 0.0 0.23297168057824957 5.219750053692247E-4 40-41 0.0 9.490454643076813E-6 0.0 0.3295370565715562 5.409559146553783E-4 42-43 0.0 9.490454643076813E-6 0.0 0.4258319546075351 5.409559146553783E-4 44-45 0.0 9.490454643076813E-6 0.0 0.5431671905872153 5.409559146553783E-4 46-47 0.0 9.490454643076813E-6 0.0 0.6798961706300228 5.457011419769168E-4 48-49 4.7452273215384066E-6 9.490454643076813E-6 0.0 0.8261345862251934 5.646820512630703E-4 50-51 9.490454643076813E-6 9.490454643076813E-6 0.0 0.9880085258448332 5.789177332276856E-4 52-53 9.490454643076813E-6 9.490454643076813E-6 0.0 1.1504424022884143 6.311152337646081E-4 54-55 9.490454643076813E-6 7.11784098230761E-5 0.0 1.328972089806654 6.785675069799922E-4 56-57 9.490454643076813E-6 7.59236371446145E-5 0.0 1.52858956754181 6.880579616230689E-4 58-59 9.490454643076813E-6 7.59236371446145E-5 0.0 1.754832515778118 6.928031889446073E-4 60-61 9.490454643076813E-6 7.59236371446145E-5 0.0 1.9898019370587354 7.022936435876841E-4 62-63 9.490454643076813E-6 7.59236371446145E-5 0.0 2.227865246552996 7.687268260892218E-4 64-65 9.490454643076813E-6 7.59236371446145E-5 0.0 2.4696393238126992 7.782172807322986E-4 66-67 9.490454643076813E-6 7.59236371446145E-5 0.0 2.741721167975068 7.877077353753755E-4 68-69 9.490454643076813E-6 7.59236371446145E-5 0.0 3.0413490567389676 7.971981900184523E-4 70-71 9.490454643076813E-6 8.066886446615291E-5 0.0 3.362529767997295 8.16179099304606E-4 72-73 9.490454643076813E-6 8.541409178769132E-5 0.0 3.681779171735756 8.921027364492205E-4 74-75 9.490454643076813E-6 8.541409178769132E-5 0.0 3.9998517590984752 9.110836457353741E-4 76-77 9.490454643076813E-6 8.541409178769132E-5 0.0 4.343463159905713 9.205741003784508E-4 78-79 9.490454643076813E-6 9.490454643076813E-5 0.0 4.715299172821464 9.205741003784508E-4 80-81 9.490454643076813E-6 1.0439500107384494E-4 0.0 5.109883805516668 9.348097823430661E-4 82-83 9.490454643076813E-6 1.0439500107384494E-4 0.0 5.51522112332248 9.775168282369117E-4 84-85 9.490454643076813E-6 1.0439500107384494E-4 0.0 5.921379365454916 9.870072828799886E-4 86-87 9.490454643076813E-6 1.0439500107384494E-4 0.0 6.340648670676764 9.964977375230655E-4 88 9.490454643076813E-6 1.0439500107384494E-4 0.0 6.665089353104987 0.001015478646809219 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13290 0.0 44.77509 1 GTATCAA 24655 0.0 41.913555 1 ATCAACG 30360 0.0 33.521202 3 CAACGCA 30485 0.0 33.484657 5 TCAACGC 31425 0.0 32.460125 4 TATCAAC 31970 0.0 32.154438 2 AACGCAG 32855 0.0 31.454449 6 TACCTGG 9190 0.0 27.466791 2 ACGCAGA 37655 0.0 27.27044 7 CGCAGAG 39230 0.0 26.079927 8 GTACATG 36020 0.0 24.532898 1 TACATGG 36655 0.0 23.76949 2 GCAGAGT 43880 0.0 23.111889 9 GTACCTG 11380 0.0 23.052168 1 ACATGGG 37600 0.0 22.199842 3 ACCTGGG 11060 0.0 21.932957 3 CATGGGG 27310 0.0 20.44519 4 AGAGTAC 40015 0.0 20.272486 10-11 GTGGTAT 7420 0.0 19.35351 1 GAGTACT 23395 0.0 18.875223 12-13 >>END_MODULE