FastQCFastQC Report
Mon 6 Jun 2016
ERR1211307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7892599
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317250.4019588477762521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254810.32284675808311053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144160.18265212764515212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145650.053.085321
GTATCAA266600.041.4916081
CAACGCA333100.032.7692575
ATCAACG334350.032.6891143
TATCAAC346400.031.6203352
TCAACGC348950.031.4020024
AACGCAG355300.030.9868416
ACGCAGA398450.027.278387
CGCAGAG406200.026.6982238
GTACATG343900.025.5873151
GTACTAG30350.025.258761
TACATGG355850.024.2782292
GCAGAGT439050.024.2633939
ACATGGG354500.023.6733633
GTGGTAT72000.022.535631
GAGTACT244800.022.4783812-13
TACCTGG50900.021.4360642
TGGTATC75100.021.2292482
CATGGGG217500.020.8909994
CAGAGTA431450.020.84147810-11
Produced by FastQC (version 0.11.5)