##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211307_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7892599 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39386683651355 31.0 31.0 34.0 28.0 34.0 2 31.413875834816896 31.0 31.0 34.0 28.0 34.0 3 31.459680138316923 31.0 31.0 34.0 28.0 34.0 4 35.06761321587477 37.0 35.0 37.0 32.0 37.0 5 35.11777489265577 37.0 35.0 37.0 32.0 37.0 6 35.1818853333357 37.0 35.0 37.0 32.0 37.0 7 35.117394536324475 37.0 35.0 37.0 32.0 37.0 8 35.12012772978837 37.0 35.0 37.0 32.0 37.0 9 36.67460224445712 39.0 37.0 39.0 32.0 39.0 10-11 36.61211135900861 39.0 37.0 39.0 32.0 39.0 12-13 36.53436143151325 39.0 37.0 39.0 32.0 39.0 14-15 37.657721683313696 40.0 37.0 41.0 32.0 41.0 16-17 37.65754841972841 40.0 37.0 41.0 32.0 41.0 18-19 37.67436113249894 40.0 37.0 41.0 32.0 41.0 20-21 37.64673151391575 40.0 37.0 41.0 32.0 41.0 22-23 37.52654087709257 40.0 37.0 41.0 31.5 41.0 24-25 37.41313191003369 40.0 37.0 41.0 31.0 41.0 26-27 37.26920942011624 40.0 36.5 41.0 31.0 41.0 28-29 37.13925438249175 40.0 36.0 41.0 30.5 41.0 30-31 36.95360660030998 39.0 36.0 41.0 30.0 41.0 32-33 36.82419206905102 39.0 36.0 41.0 30.0 41.0 34-35 36.680643980518965 39.0 35.0 41.0 30.0 41.0 36-37 36.58159175450317 39.0 35.0 41.0 30.0 41.0 38-39 36.43406557206315 39.0 35.0 41.0 29.5 41.0 40-41 36.26852555159587 39.0 35.0 40.0 29.0 41.0 42-43 36.11741613884096 39.0 35.0 40.0 28.0 41.0 44-45 35.96846957510448 39.0 35.0 40.0 27.5 41.0 46-47 35.789419429518716 39.0 35.0 40.0 27.0 41.0 48-49 35.53764159309247 38.0 34.0 40.0 26.0 41.0 50-51 34.8660310120912 37.5 33.5 39.5 25.0 40.5 52-53 35.10352363017556 38.0 34.0 40.0 26.0 40.5 54-55 35.46057154303671 38.0 34.0 40.0 26.0 41.0 56-57 35.361327681794045 38.0 34.0 40.0 26.0 41.0 58-59 35.13928890850783 38.0 34.0 40.0 26.0 41.0 60-61 34.87966809158808 37.5 34.0 40.0 26.0 41.0 62-63 34.61292617298814 37.0 33.5 40.0 25.0 41.0 64-65 34.34203023617442 37.0 33.0 40.0 25.0 41.0 66-67 34.12113519260259 36.0 33.0 39.5 24.5 41.0 68-69 33.78098988685476 36.0 33.0 39.0 23.5 41.0 70-71 33.44608410993641 35.0 32.0 39.0 23.5 40.5 72-73 33.08899520930938 35.0 32.0 38.0 23.0 40.0 74-75 32.71696439917953 35.0 32.0 37.0 22.0 39.5 76-77 32.36557343151476 35.0 31.0 37.0 22.0 39.0 78-79 32.0006514330704 35.0 31.0 36.0 20.5 39.0 80-81 31.665481294564692 35.0 31.0 36.0 20.0 37.5 82-83 31.35225215420168 34.5 30.5 35.0 20.0 37.0 84-85 31.042759045531135 34.0 30.0 35.0 19.5 37.0 86-87 30.79178360638872 34.0 30.0 35.0 19.0 36.0 88-89 30.530805631959765 34.0 29.5 35.0 18.0 36.0 90-91 30.272721064379425 34.0 29.0 35.0 18.0 36.0 92-93 30.03327484393924 34.0 29.0 35.0 17.0 35.0 94-95 29.81301609267112 34.0 29.0 35.0 16.0 35.0 96-97 29.612263590231812 34.0 29.0 35.0 15.0 35.0 98-99 29.37688174706456 34.0 27.5 35.0 12.0 35.0 100 27.71920010125942 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3149228823433603 1101 2 -0.31228666909540337 1101 3 -0.21113219211751044 1101 4 -0.2617692714070543 1101 5 -0.2301661267442654 1101 6 -0.25052125556857163 1101 7 -0.3146179695840132 1101 8 -0.41388466579478234 1101 9 -0.4658121039502916 1101 10-11 -0.32503907328581505 1101 12-13 -0.3423002142105034 1101 14-15 -0.4165317244948099 1101 16-17 -0.3983580836802716 1101 18-19 -0.4431127462781177 1101 20-21 -0.5912096916258918 1101 22-23 -0.4693626824943067 1101 24-25 -0.5254135241234223 1101 26-27 -0.5707358147624149 1101 28-29 -0.6710155506953797 1101 30-31 -0.6328877185515438 1101 32-33 -0.6592209710172554 1101 34-35 -0.5670193999298831 1101 36-37 -0.7205354117841622 1101 38-39 -0.665682580297819 1101 40-41 -0.7259560020276012 1101 42-43 -0.7680593690585766 1101 44-45 -0.7750622113823766 1101 46-47 -0.7787463857079047 1101 48-49 -0.7529786639926144 1101 50-51 -0.7890263807147377 1101 52-53 -0.6036796338547887 1101 54-55 -0.5825545627849777 1101 56-57 -0.44359786197336604 1101 58-59 -0.5443071983172345 1101 60-61 -0.49800112075801906 1101 62-63 -0.4465584849574 1101 64-65 -0.42331312792062903 1101 66-67 -0.5172700428489421 1101 68-69 -0.4475626041437337 1101 70-71 -0.555021420487698 1101 72-73 -0.5083433138374644 1101 74-75 -0.524912795148758 1101 76-77 -0.5603532257890933 1101 78-79 -0.5338753933529539 1101 80-81 -0.6050579487685148 1101 82-83 -0.6961730262037129 1101 84-85 -0.6443091158629812 1101 86-87 -0.6869952174982892 1101 88-89 -0.708537440228465 1101 90-91 -0.7800290301034032 1101 92-93 -0.7632930864101226 1101 94-95 -0.8796473912733447 1101 96-97 -0.8521425576287314 1101 98-99 -0.8174791916432049 1101 100 -0.9163721817577795 1102 1 -0.3874425278055966 1102 2 -0.33385957472177097 1102 3 -0.3622758973781792 1102 4 -0.2502843891163735 1102 5 -0.29847007314827323 1102 6 -0.28409054248464116 1102 7 -0.2899375275968694 1102 8 -0.2977775042701296 1102 9 -0.40203205622834304 1102 10-11 -0.4385891345263815 1102 12-13 -0.42041419132581126 1102 14-15 -0.5544916417336694 1102 16-17 -0.6195892253181867 1102 18-19 -0.6026209345342721 1102 20-21 -0.6830344423635779 1102 22-23 -0.6481221095857066 1102 24-25 -0.6790359911469466 1102 26-27 -0.6970368982579984 1102 28-29 -0.7492115580405354 1102 30-31 -0.6648524124434232 1102 32-33 -0.7031229788609465 1102 34-35 -0.8811301690200608 1102 36-37 -0.8157138360087544 1102 38-39 -1.0101482029214282 1102 40-41 -0.983536658663219 1102 42-43 -0.8992069694201135 1102 44-45 -0.9863905884459712 1102 46-47 -1.0173944978006162 1102 48-49 -1.0688768701658589 1102 50-51 -0.9282239490721835 1102 52-53 -0.7688714745379244 1102 54-55 -0.7687059746615219 1102 56-57 -0.875848902431521 1102 58-59 -0.870924315288768 1102 60-61 -0.9402490063144953 1102 62-63 -0.8793312172452801 1102 64-65 -0.9833898346602155 1102 66-67 -0.8499257735137675 1102 68-69 -0.842827391131685 1102 70-71 -0.8295691931475488 1102 72-73 -0.9456114559616466 1102 74-75 -0.9241986190248213 1102 76-77 -0.9626891439685714 1102 78-79 -0.8913310241233958 1102 80-81 -1.0421509088705747 1102 82-83 -0.9806939380845314 1102 84-85 -1.0307934409146284 1102 86-87 -0.9658155740346821 1102 88-89 -1.0094480756184154 1102 90-91 -1.1214358283453514 1102 92-93 -1.1144968666826003 1102 94-95 -1.1624159489121055 1102 96-97 -1.1722272774656624 1102 98-99 -1.1951470896223952 1102 100 -1.2289748762685662 1103 1 -0.35499848958689384 1103 2 -0.40694337947839543 1103 3 -0.36861424204688475 1103 4 -0.24146261617050868 1103 5 -0.28769353787961904 1103 6 -0.4199069624155811 1103 7 -0.3376360686267006 1103 8 -0.28457890697657007 1103 9 -0.4349980931145865 1103 10-11 -0.5090005251731995 1103 12-13 -0.6213725888620232 1103 14-15 -0.6500489201000121 1103 16-17 -0.6582578447893113 1103 18-19 -0.5838141285134526 1103 20-21 -0.6606020043168499 1103 22-23 -0.6742465577146461 1103 24-25 -0.6674929786880526 1103 26-27 -0.6193678370677702 1103 28-29 -0.6175485516538686 1103 30-31 -0.7297922283872964 1103 32-33 -0.7750117064700319 1103 34-35 -0.8192248190031464 1103 36-37 -0.8490858970562485 1103 38-39 -0.8491369338006578 1103 40-41 -0.8397880043099235 1103 42-43 -0.8244227570654843 1103 44-45 -0.926830252470964 1103 46-47 -0.9155700151696138 1103 48-49 -0.9744160429932194 1103 50-51 -0.9833317507094179 1103 52-53 -0.759889856791979 1103 54-55 -0.7454985212153957 1103 56-57 -0.7676225544841415 1103 58-59 -0.7294848666579696 1103 60-61 -0.7031652190651627 1103 62-63 -0.7997452770746065 1103 64-65 -0.6648574283125726 1103 66-67 -0.7073965296371654 1103 68-69 -0.785149214354341 1103 70-71 -0.7278448445471426 1103 72-73 -0.8201430620534396 1103 74-75 -0.7525484691628037 1103 76-77 -0.6770852766882172 1103 78-79 -0.6672784263057956 1103 80-81 -0.6914964443668659 1103 82-83 -0.7482239133216844 1103 84-85 -0.704049057570856 1103 86-87 -0.854549136054441 1103 88-89 -0.9379939235791497 1103 90-91 -0.9494147369504091 1103 92-93 -0.9782603056310464 1103 94-95 -1.0415106211648393 1103 96-97 -1.0671238844017523 1103 98-99 -1.088209523683723 1103 100 -1.2031136272244929 1104 1 -0.40725396534344327 1104 2 -0.4476281687123844 1104 3 -0.44307358630486604 1104 4 -0.36093602699347116 1104 5 -0.23473686731571775 1104 6 -0.29949997906319226 1104 7 -0.3187705882573795 1104 8 -0.3442237393624694 1104 9 -0.48414490111080966 1104 10-11 -0.5189802474299654 1104 12-13 -0.48700141332651015 1104 14-15 -0.6461542707742254 1104 16-17 -0.6326813990757145 1104 18-19 -0.6729426945560277 1104 20-21 -0.6145502964713003 1104 22-23 -0.6631373643606722 1104 24-25 -0.6648651401005239 1104 26-27 -0.7666215443901052 1104 28-29 -0.7114442420992546 1104 30-31 -0.8110178994541286 1104 32-33 -0.8647723438054769 1104 34-35 -0.8135143028374827 1104 36-37 -0.8569654483978653 1104 38-39 -0.8183840021113511 1104 40-41 -0.9199714971566522 1104 42-43 -0.9034603918215041 1104 44-45 -0.9337909655343992 1104 46-47 -0.8395503741966408 1104 48-49 -0.857519689441709 1104 50-51 -0.854531569646646 1104 52-53 -0.7847936364061212 1104 54-55 -0.8032580931910189 1104 56-57 -0.7525904423501757 1104 58-59 -0.7381547744710275 1104 60-61 -0.686234343724685 1104 62-63 -0.7334072274197894 1104 64-65 -0.7986476055316984 1104 66-67 -0.7824218354976438 1104 68-69 -0.7437536271243133 1104 70-71 -0.7488445768893115 1104 72-73 -0.7130956696799657 1104 74-75 -0.7584630395851732 1104 76-77 -0.7491457141671489 1104 78-79 -0.7318774130257211 1104 80-81 -0.8174655319509547 1104 82-83 -0.8774932031301255 1104 84-85 -0.8637858191539678 1104 86-87 -0.8658601476020529 1104 88-89 -0.80083511196435 1104 90-91 -0.8280007042526023 1104 92-93 -0.8167522040289228 1104 94-95 -0.8747975156813652 1104 96-97 -0.8706347799376637 1104 98-99 -0.8647279946771995 1104 100 -0.9393058460318429 1105 1 -0.4392550641532509 1105 2 -0.4755842811603408 1105 3 -0.4364412154721862 1105 4 -0.314623914641615 1105 5 -0.29279634488318607 1105 6 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68-69 -0.6485010586274456 1105 70-71 -0.6579953526415068 1105 72-73 -0.6839352373695178 1105 74-75 -0.6934544439167851 1105 76-77 -0.674382286818421 1105 78-79 -0.6918380833923301 1105 80-81 -0.75860517660929 1105 82-83 -0.6680652210526539 1105 84-85 -0.6966020953176439 1105 86-87 -0.7935573189773066 1105 88-89 -0.6939110904941401 1105 90-91 -0.770588977381518 1105 92-93 -0.7832528334709465 1105 94-95 -0.7512657192344321 1105 96-97 -0.8485152985550464 1105 98-99 -0.9095622425757561 1105 100 -0.8329284071791889 1106 1 -0.4777709270087769 1106 2 -0.47501221976131447 1106 3 -0.5031323736800175 1106 4 -0.385829095820327 1106 5 -0.3441778448961301 1106 6 -0.3319493964395761 1106 7 -0.43474409136546655 1106 8 -0.4268581914837952 1106 9 -0.4452391597815435 1106 10-11 -0.5661073618588759 1106 12-13 -0.6202789513678155 1106 14-15 -0.7059249038312245 1106 16-17 -0.6645015399125285 1106 18-19 -0.6696228148470169 1106 20-21 -0.6765497393905306 1106 22-23 -0.7038505656679348 1106 24-25 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22-23 0.9386843051399438 2311 24-25 0.9359038725057971 2311 26-27 0.985643233322385 2311 28-29 0.989251505933872 2311 30-31 0.9850459957468729 2311 32-33 1.0176705698430837 2311 34-35 1.0859013897126104 2311 36-37 1.0368979854844085 2311 38-39 1.0865132086566334 2311 40-41 1.0834171653916869 2311 42-43 1.0873772032718563 2311 44-45 1.1572260481396341 2311 46-47 1.0926169008595608 2311 48-49 1.1679211979750264 2311 50-51 1.1298301868311427 2311 52-53 1.040949885805297 2311 54-55 0.9737167136756426 2311 56-57 0.9689515846269572 2311 58-59 0.9514645682429403 2311 60-61 0.9761267428312905 2311 62-63 0.9185052888515912 2311 64-65 0.945534561201697 2311 66-67 0.9670774022821362 2311 68-69 1.011255999210114 2311 70-71 1.0046711584733714 2311 72-73 0.920340914377455 2311 74-75 0.9096722768207215 2311 76-77 0.9625922064406112 2311 78-79 0.9026293234584131 2311 80-81 0.9445146828226889 2311 82-83 1.0014833743060727 2311 84-85 1.0120271543628867 2311 86-87 1.0205783556556902 2311 88-89 1.1440776855355317 2311 90-91 1.1770785250513214 2311 92-93 1.1048471736284498 2311 94-95 1.0861502351839896 2311 96-97 1.053898647254055 2311 98-99 1.090468492609883 2311 100 1.2283322289266039 2312 1 0.5607616868793279 2312 2 0.4936514889631951 2312 3 0.563204561473075 2312 4 0.49017909981512275 2312 5 0.4476946470526215 2312 6 0.4451560702184594 2312 7 0.4856061990145477 2312 8 0.5306927632508831 2312 9 0.5901766209817865 2312 10-11 0.6515403783003038 2312 12-13 0.6916617117579804 2312 14-15 0.8752180715566098 2312 16-17 0.7816238090754482 2312 18-19 0.7893845256659517 2312 20-21 0.8164634178790919 2312 22-23 0.8064427126414344 2312 24-25 0.8289325137334131 2312 26-27 0.8889479802222695 2312 28-29 0.9021594558742763 2312 30-31 0.8819055631282708 2312 32-33 0.852250329323347 2312 34-35 0.8991113183967556 2312 36-37 0.9501038140154847 2312 38-39 0.9648202655681644 2312 40-41 1.0011488877890713 2312 42-43 1.090964155027521 2312 44-45 1.0735171183590921 2312 46-47 1.056937971026322 2312 48-49 1.0537966716920835 2312 50-51 1.0426620498804766 2312 52-53 0.8992773178664848 2312 54-55 0.8912162587303669 2312 56-57 0.9070481533704537 2312 58-59 0.857911530845783 2312 60-61 0.8314445143390472 2312 62-63 0.8571288084211091 2312 64-65 0.8156961923879678 2312 66-67 0.8578538527154009 2312 68-69 0.916134859093745 2312 70-71 0.8131878585873764 2312 72-73 0.8827345479853292 2312 74-75 0.8440084547460032 2312 76-77 0.9083176710117158 2312 78-79 0.9355193258785164 2312 80-81 0.9097536062462233 2312 82-83 0.9174956905731086 2312 84-85 0.9435967875236244 2312 86-87 0.9626029439655035 2312 88-89 0.9824388305937255 2312 90-91 0.9726130890359173 2312 92-93 0.9821453882293802 2312 94-95 0.9973660545390075 2312 96-97 1.022419739683503 2312 98-99 1.032698676661827 2312 100 1.18695406279922 2313 1 0.4492048905133146 2313 2 0.44880258653399707 2313 3 0.4828669189262307 2313 4 0.3610290618394387 2313 5 0.38700201862139494 2313 6 0.36426147120606345 2313 7 0.4193466726939832 2313 8 0.37084265516264026 2313 9 0.5274952633339041 2313 10-11 0.5629341814467352 2313 12-13 0.6080843225295993 2313 14-15 0.7820735181365635 2313 16-17 0.7128621074690003 2313 18-19 0.6984170242827474 2313 20-21 0.7128093025114168 2313 22-23 0.6416874740598928 2313 24-25 0.6350050866416339 2313 26-27 0.6572389320903156 2313 28-29 0.7174687946743816 2313 30-31 0.8224819996193204 2313 32-33 0.8493903979911792 2313 34-35 0.8280265242135627 2313 36-37 0.8521002487829676 2313 38-39 0.8343146565166606 2313 40-41 0.8404333789714826 2313 42-43 0.7662019603968275 2313 44-45 0.8547130666335576 2313 46-47 0.9063750049796866 2313 48-49 0.9492146737302392 2313 50-51 0.9097577781794755 2313 52-53 0.7650372769985339 2313 54-55 0.7547526260763817 2313 56-57 0.7479458626744062 2313 58-59 0.7513341133590359 2313 60-61 0.7697736622529661 2313 62-63 0.7708296099761753 2313 64-65 0.7364403446140955 2313 66-67 0.7107786192166259 2313 68-69 0.7220738948528336 2313 70-71 0.665063346149438 2313 72-73 0.6921582635467303 2313 74-75 0.713151230228334 2313 76-77 0.7951007892773774 2313 78-79 0.7766728108132952 2313 80-81 0.7735576794930275 2313 82-83 0.7470431164840328 2313 84-85 0.8189904513432147 2313 86-87 0.8176419999265185 2313 88-89 0.758676277961353 2313 90-91 0.8482325156699488 2313 92-93 0.9107742792635349 2313 94-95 0.938440954083795 2313 96-97 0.9590984166231173 2313 98-99 0.9827305538753031 2313 100 1.132743243606999 2314 1 0.28997319512248154 2314 2 0.18627827326854884 2314 3 0.285105699948911 2314 4 0.1815686121407012 2314 5 0.18546180804015222 2314 6 0.2053737394033348 2314 7 0.27183467598128885 2314 8 0.2940406545901766 2314 9 0.3950187309205546 2314 10-11 0.4150764819026804 2314 12-13 0.44412507033373316 2314 14-15 0.46389152336097794 2314 16-17 0.44438350621450695 2314 18-19 0.45027575580791535 2314 20-21 0.473074813127937 2314 22-23 0.5540050839113988 2314 24-25 0.5907506970627097 2314 26-27 0.577378144734439 2314 28-29 0.5347256927390092 2314 30-31 0.5784790910624977 2314 32-33 0.6585744968445226 2314 34-35 0.653265713077495 2314 36-37 0.6402631534438896 2314 38-39 0.6917684477597632 2314 40-41 0.7133495396764715 2314 42-43 0.6094444175924636 2314 44-45 0.7151236617601384 2314 46-47 0.4996138255757998 2314 48-49 0.5249523026059038 2314 50-51 0.6229278792417077 2314 52-53 0.5888143043869576 2314 54-55 0.5688509002772548 2314 56-57 0.5461198740975775 2314 58-59 0.5511506254544116 2314 60-61 0.35733127863861114 2314 62-63 0.5350448968492216 2314 64-65 0.592197921932268 2314 66-67 0.6412241582248299 2314 68-69 0.5784372483378988 2314 70-71 0.5652158529365536 2314 72-73 0.5930786801122068 2314 74-75 0.6079167811410926 2314 76-77 0.5150932640009742 2314 78-79 0.5750287425634042 2314 80-81 0.716143881809753 2314 82-83 0.5952225624776766 2314 84-85 0.6494702060096245 2314 86-87 0.6668746593550416 2314 88-89 0.5542321184215169 2314 90-91 0.5455872486522892 2314 92-93 0.7190135398655997 2314 94-95 0.6728137630890458 2314 96-97 0.6673181796306658 2314 98-99 0.7505239836377022 2314 100 0.734894917906459 2315 1 0.20971821347071895 2315 2 0.21199699086700008 2315 3 0.19811322150283317 2315 4 0.13042901696701392 2315 5 0.14248618563744486 2315 6 0.13842702555794517 2315 7 0.14304764816490945 2315 8 0.1664459240124927 2315 9 2.634922969448894E-4 2315 10-11 0.22528415320173423 2315 12-13 0.19544828253525282 2315 14-15 0.3275079914992247 2315 16-17 0.14900030771814698 2315 18-19 0.3170026647933497 2315 20-21 0.20985405535225965 2315 22-23 0.17283266450507995 2315 24-25 0.323761794420399 2315 26-27 0.44805813408157746 2315 28-29 0.3659271270417648 2315 30-31 0.43170301057429583 2315 32-33 0.4494506109924217 2315 34-35 0.33923465916718243 2315 36-37 0.5122908662031378 2315 38-39 0.5838735780277702 2315 40-41 0.6057651991375792 2315 42-43 0.567671961165658 2315 44-45 0.6224618711816845 2315 46-47 0.6776669594172802 2315 48-49 0.5859434224275191 2315 50-51 0.5306828815753093 2315 52-53 0.2965631495647827 2315 54-55 0.47021525210325876 2315 56-57 0.4140865946921437 2315 58-59 0.4158091728189248 2315 60-61 0.4780589600907277 2315 62-63 0.47571298293634356 2315 64-65 0.45693364917983104 2315 66-67 0.4439107764890906 2315 68-69 0.4783521210273918 2315 70-71 0.36122978288751284 2315 72-73 0.36509053356345333 2315 74-75 0.10643346780882013 2315 76-77 0.36272615537202313 2315 78-79 0.44115767711089404 2315 80-81 0.4720166569178552 2315 82-83 0.4040045010536524 2315 84-85 0.4460790458239572 2315 86-87 0.37615280264123285 2315 88-89 0.3515097356565171 2315 90-91 0.5405588796324423 2315 92-93 0.6703856678475759 2315 94-95 0.5757262367283182 2315 96-97 0.5672425745834708 2315 98-99 0.7203349565855142 2315 100 0.634331329179993 2316 1 0.03432004521481957 2316 2 0.0849829730099465 2316 3 0.06801366604616632 2316 4 -0.10050836829288556 2316 5 -0.06303875331165898 2316 6 -0.13759028398260398 2316 7 -0.11512514018705389 2316 8 -0.08769286740645299 2316 9 -0.01882680067659237 2316 10-11 -0.07386389398528337 2316 12-13 -0.00531862533676275 2316 14-15 -0.16304437472401645 2316 16-17 0.026177529313663683 2316 18-19 0.07811572947341006 2316 20-21 0.13551061437930656 2316 22-23 0.08342438396889662 2316 24-25 -0.01110823421975482 2316 26-27 -0.10306409772350378 2316 28-29 -0.030256775417420556 2316 30-31 -0.6280803162802897 2316 32-33 -0.4904502672496349 2316 34-35 -0.560107154900912 2316 36-37 -0.38033907516349785 2316 38-39 -0.18502881625651213 2316 40-41 0.0022306315207742955 2316 42-43 0.019254978618540974 2316 44-45 -0.16035023883297583 2316 46-47 -0.20525315077555462 2316 48-49 -0.09546551502963752 2316 50-51 0.06522457376294 2316 52-53 -0.2697974226788702 2316 54-55 -0.20531277448991858 2316 56-57 -0.27189762906765935 2316 58-59 -0.6259695995147112 2316 60-61 -0.18677491244688582 2316 62-63 -0.19445920123138194 2316 64-65 -0.5114385238203667 2316 66-67 -0.8029569638556495 2316 68-69 -0.8413782667816037 2316 70-71 -0.6295226931675799 2316 72-73 -0.7960377864906363 2316 74-75 -0.6002522086770412 2316 76-77 -0.3165937464315647 2316 78-79 -0.27956640258436494 2316 80-81 -0.28315910857910964 2316 82-83 -0.06397793119679918 2316 84-85 -0.03196811061474136 2316 86-87 0.12789324866787055 2316 88-89 0.08912194317212041 2316 90-91 -0.488099944914417 2316 92-93 -0.5640631105901406 2316 94-95 -0.17412507496759844 2316 96-97 0.006022519122641512 2316 98-99 0.07151176321604069 2316 100 -0.020759227776636635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 232.0 1 594.0 2 309.0 3 294.0 4 308.0 5 260.0 6 347.0 7 781.0 8 1943.0 9 3481.0 10 5635.0 11 9121.0 12 14351.0 13 18837.0 14 22263.0 15 25398.0 16 29617.0 17 35695.0 18 43151.0 19 52090.0 20 63526.0 21 77441.0 22 86702.0 23 87210.0 24 85519.0 25 87801.0 26 95775.0 27 108835.0 28 126587.0 29 151079.0 30 183303.0 31 225752.0 32 284494.0 33 369213.0 34 509645.0 35 713140.0 36 1056939.0 37 1537491.0 38 1442549.0 39 334665.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4198098232835 19.849756566655188 13.766045708971655 22.964387901089655 2 15.59141442065706 20.31291481050362 40.75575310656402 23.339917662275294 3 18.762668192731784 26.260362811337366 29.67079394458645 25.306175051344393 4 12.614226214380006 16.966122853302437 38.72452890537353 31.695122026944023 5 13.276221684372494 37.73743383837955 34.74356467475418 14.242779802493773 6 30.201702227772596 37.4863289895393 18.213157735953907 14.098811046734195 7 27.295597101604695 32.57881912054595 22.404837064453844 17.720746713395513 8 25.876579688924355 35.08209870096158 20.30738720998265 18.733934400131414 9 26.398838022077324 16.209541324170672 21.052941098188274 36.33867955556372 10-11 24.78654876162634 26.444162993884735 28.06562241256223 20.703665831926696 12-13 25.43769009114996 24.243635818168027 28.2410689598552 22.077605130826818 14-15 23.068863377784908 25.577608765552128 26.064981706959262 25.2885461497037 16-17 21.927288711351515 28.483020010091682 26.97724074355971 22.612450534997095 18-19 21.616350111911974 27.592756261563363 29.31379499024232 21.477098636282346 20-21 22.647953593546003 26.733230516896928 29.211751851497624 21.40706403805945 22-23 22.734271952203997 26.511515826652026 28.835647429688986 21.91856479145499 24-25 22.253352394330108 26.761359274495405 29.198165838659452 21.787122492515035 26-27 22.11201321493894 26.954444020807443 28.93764200687306 21.995900757380554 28-29 21.850395435077463 26.873226195887113 29.273059524773092 22.00331884426234 30-31 22.378895937706066 26.73258422009752 29.10209088993831 21.786428952258102 32-33 22.26271894883013 27.08050269263409 28.754708227728965 21.902070130806813 34-35 22.412626256096203 27.006337744833708 28.933222342306163 21.647813656763926 36-37 22.407167186524 26.764557357367103 28.754868592369657 22.073406863739244 38-39 22.28252110057576 26.990337806323495 28.88400075371309 21.843140339387652 40-41 22.249981570652036 26.59078867277446 29.23785154179323 21.92137821478028 42-43 22.138625135417584 26.638764300733747 28.85325814497419 22.36935241887448 44-45 22.09226642947625 27.11128544291152 28.73345439645756 22.06299373115467 46-47 22.171251632114668 26.938281565658645 28.377578817240302 22.51288798498638 48-49 22.378176651659825 26.947580299335222 28.307520729202924 22.36672231980203 50-51 22.329981702763043 27.036099249912798 28.101474205725797 22.53244484159836 52-53 22.17880180569261 27.32031456167872 27.992070602370646 22.508813030258025 54-55 22.17616618405646 27.23626544757253 28.001252170985353 22.586316197385653 56-57 22.363854661677333 27.230766383318684 27.774109193136464 22.631269761867514 58-59 22.090256856346958 27.14471256201531 28.16920075368175 22.595829827955985 60-61 22.303238530477888 27.421647081223092 27.87603123132838 22.399083156970644 62-63 22.25236979531794 27.19924383581927 27.947972892284888 22.600413476577902 64-65 21.984603248904 27.03330638431789 28.055560397434064 22.926529969344042 66-67 22.228206277549404 27.000543572232992 27.79055507750721 22.980695072710393 68-69 22.012646587229227 27.10561151582579 27.902162183816305 22.97957971312868 70-71 22.194994390651164 26.849349922139744 27.972950128296542 22.98270555891255 72-73 22.386357031072862 27.082032167884208 27.722844200348113 22.808766600694817 74-75 22.274901852910567 27.300368756710576 27.680109574871302 22.74461981550756 76-77 22.211771136605847 27.191234542094346 27.6726126723864 22.924381648913414 78-79 22.1986472194023 27.11618630098085 27.644373561668278 23.040792917948576 80-81 22.1491335109336 27.435995380685824 27.525906941027927 22.888964167352647 82-83 22.560757093952844 27.096602242353985 27.777953840897958 22.564686822795217 84-85 22.470146836067592 27.241607522574522 27.41621118917546 22.872034452182422 86-87 22.113056079148787 27.43640513147708 27.699350420424697 22.751188368949435 88-89 22.425995559787467 27.01256914972079 27.659076924403237 22.902358366088507 90-91 22.558165865493727 27.016885363170083 27.651192706752447 22.773756064583743 92-93 22.62787295047593 27.164531797597814 27.47648098042548 22.731114271500775 94-95 22.2837860190213 26.907457155538722 27.724535078864037 23.08422174657594 96-97 22.51562713072383 26.887616460550223 27.538751397976718 23.05800501074923 98-99 22.34982424570464 27.06654189942965 27.42546855712828 23.158165297737433 100 22.757805024185206 26.82195249832196 27.07231052870009 23.347931948792745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3207.0 1 2229.0 2 2222.5 3 2920.0 4 2095.0 5 1723.0 6 2510.5 7 4440.5 8 7143.5 9 8693.5 10 7603.5 11 6103.0 12 6148.5 13 6735.0 14 7436.5 15 8124.5 16 8808.0 17 9507.5 18 10266.5 19 11028.0 20 11947.0 21 13096.0 22 14852.5 23 17354.0 24 19994.5 25 23884.5 26 30241.0 27 37816.5 28 45087.0 29 56414.0 30 73519.0 31 90709.0 32 113172.5 33 140707.5 34 167451.0 35 192329.0 36 221005.0 37 252159.0 38 264819.0 39 264972.0 40 260417.0 41 253480.0 42 253741.0 43 261516.0 44 271642.0 45 288033.0 46 299996.5 47 303092.0 48 308827.0 49 311949.5 50 305880.0 51 296003.0 52 286408.0 53 274650.0 54 261459.0 55 241147.0 56 218673.5 57 191161.5 58 164146.0 59 144470.0 60 121184.0 61 96931.0 62 77208.0 63 60770.5 64 45487.0 65 32284.5 66 23102.0 67 17158.0 68 13054.5 69 9892.5 70 7676.0 71 5847.5 72 4321.5 73 3307.5 74 2587.5 75 1896.5 76 1307.5 77 866.0 78 596.0 79 401.0 80 276.0 81 201.5 82 152.0 83 114.0 84 87.5 85 66.0 86 57.5 87 48.5 88 34.0 89 30.0 90 27.5 91 18.0 92 12.0 93 10.0 94 6.5 95 5.0 96 3.5 97 3.0 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02455464923531526 2 0.11058461224243117 3 0.01621772498514114 4 0.017104631820266048 5 0.01908116705283013 6 0.04615716571942905 7 0.04665129952757007 8 0.038909869866694105 9 0.03299293426664651 10-11 0.03695233978059699 12-13 0.03545726825852929 14-15 0.0309150382529253 16-17 0.03712338609879965 18-19 0.036946004731774666 20-21 0.04792464434085654 22-23 0.04736716004449232 24-25 0.06700581139368667 26-27 0.05773129991780908 28-29 0.05080075650619017 30-31 0.048209721537860975 32-33 0.044142620193931045 34-35 0.046537268648768296 36-37 0.04124116783330814 38-39 0.04631554193998707 40-41 0.05531131126768255 42-43 0.056996434254419866 44-45 0.059587469222749066 46-47 0.06114589123303996 48-49 0.06022730915380346 50-51 0.05384157994090413 52-53 0.06320478210029422 54-55 0.04941338081410192 56-57 0.05449408996960317 58-59 0.0522451476376793 60-61 0.04801333502436903 62-63 0.05530497621886023 64-65 0.053803569647970215 66-67 0.051225204777285656 68-69 0.056546645788035095 70-71 0.056109527419294966 72-73 0.058770247924669686 74-75 0.04978081364579652 76-77 0.050655050383276785 78-79 0.06045537091140701 80-81 0.061424633381222074 82-83 0.05085777194559105 84-85 0.056362929372187794 86-87 0.05405063655204072 88-89 0.062254524776946096 90-91 0.06858323855044454 92-93 0.07269468523613071 94-95 0.07626765277191962 96-97 0.07291007689608961 98-99 0.08169045456382619 100 0.12528192551021533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7892599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.098750541905 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34915926714878 40.4144702891909 2 15.22907103501739 16.78205571886748 3 5.35977758287953 8.859511439975213 4 2.2960015117997163 5.060272581699573 5 1.1723115698496194 3.229645137226661 6 0.6727030051762689 2.223905704259824 7 0.4481577971282096 1.7285054267163824 8 0.28235034558909566 1.24457210056274 9 0.2018608715025991 1.0010053622783923 >10 0.8907073337719581 8.552266617030408 >50 0.05224359319785284 1.9967380413676499 >100 0.03965037186117493 4.445622026526514 >500 0.0041039131528136565 1.473292942600463 >1k 0.001768342140837131 1.5597071938837435 >5k 3.336494605107666E-5 0.11175432016180026 >10k+ 1.0009483815322997E-4 1.316675097652321 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31725 0.4019588477762521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25481 0.32284675808311053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14416 0.18265212764515212 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5340195289283037E-5 2 0.0 0.0 0.0 0.0 2.5340195289283037E-5 3 0.0 0.0 0.0 0.0 2.5340195289283037E-5 4 0.0 0.0 0.0 0.0 2.5340195289283037E-5 5 0.0 5.0680390578566074E-5 0.0 0.0 2.5340195289283037E-5 6 0.0 5.0680390578566074E-5 0.0 0.0 3.801029293392455E-5 7 0.0 6.335048822320758E-5 0.0 0.0 3.801029293392455E-5 8 0.0 6.335048822320758E-5 0.0 0.0 3.801029293392455E-5 9 0.0 6.335048822320758E-5 0.0 0.0 6.335048822320758E-5 10-11 0.0 6.335048822320758E-5 0.0 6.335048822320759E-6 6.335048822320758E-5 12-13 0.0 7.60205858678491E-5 0.0 1.2670097644641519E-5 6.335048822320758E-5 14-15 0.0 8.869068351249063E-5 0.0 1.2670097644641519E-5 8.869068351249063E-5 16-17 0.0 8.869068351249063E-5 0.0 1.2670097644641519E-5 1.1403087880177367E-4 18-19 0.0 8.869068351249063E-5 0.0 4.4345341756245316E-5 1.1403087880177367E-4 20-21 6.335048822320759E-6 8.869068351249063E-5 0.0 1.0769582997945289E-4 1.1403087880177367E-4 22-23 1.2670097644641519E-5 1.1403087880177367E-4 0.0 3.9277302698388704E-4 1.4570612291337745E-4 24-25 1.2670097644641519E-5 1.2036592762409442E-4 0.0 0.0012036592762409443 1.6471126938033973E-4 26-27 1.2670097644641519E-5 1.2670097644641517E-4 0.0 0.0028761121653336245 1.6471126938033973E-4 28-29 1.2670097644641519E-5 1.2670097644641517E-4 0.0 0.0065441054334573435 1.6471126938033973E-4 30-31 1.2670097644641519E-5 1.2670097644641517E-4 0.0 0.017839497483655258 1.710463182026605E-4 32-33 1.2670097644641519E-5 1.2670097644641517E-4 0.0 0.04411727999864176 1.7738136702498126E-4 34-35 1.2670097644641519E-5 1.393710740910567E-4 0.0 0.0856308549313097 1.7738136702498126E-4 36-37 1.2670097644641519E-5 1.9005146466962277E-4 0.0 0.1493234357909226 1.7738136702498126E-4 38-39 1.2670097644641519E-5 2.4073185524818883E-4 0.0 0.24073185524818883 1.8371641584730202E-4 40-41 1.2670097644641519E-5 2.7240709935979265E-4 0.0 0.3390961583123633 1.9005146466962277E-4 42-43 1.2670097644641519E-5 2.914122458267549E-4 0.0 0.4375618221576948 1.9005146466962277E-4 44-45 1.2670097644641519E-5 2.9774729464907565E-4 0.0 0.560607475433631 1.9005146466962277E-4 46-47 1.2670097644641519E-5 3.1675244111603796E-4 0.0 0.7081761027007707 1.9005146466962277E-4 48-49 1.2670097644641519E-5 3.1675244111603796E-4 0.0 0.8641766799504194 1.9005146466962277E-4 50-51 1.2670097644641519E-5 3.1675244111603796E-4 0.0 1.0234461423923855 1.9005146466962277E-4 52-53 1.2670097644641519E-5 3.2942253876067947E-4 0.0 1.1772547927495114 1.9638651349194352E-4 54-55 1.2670097644641519E-5 4.117781734508493E-4 0.0 1.3524252277355027 2.027215623142643E-4 56-57 1.2670097644641519E-5 4.434534175624531E-4 0.0 1.557357722088757 2.1539165995890583E-4 58-59 1.2670097644641519E-5 4.941338081410192E-4 0.0 1.7856665465963746 2.2806175760354733E-4 60-61 1.2670097644641519E-5 5.131389546079814E-4 0.0 2.021824496594848 2.2806175760354733E-4 62-63 1.2670097644641519E-5 5.194740034303023E-4 0.0 2.2524836242155466 2.4073185524818883E-4 64-65 1.2670097644641519E-5 5.321441010749438E-4 0.0 2.49650336980252 2.4073185524818883E-4 66-67 1.2670097644641519E-5 5.574842963642268E-4 0.0 2.768023562327188 2.470669040705096E-4 68-69 1.2670097644641519E-5 5.574842963642268E-4 0.0 3.0682795363099027 2.5340195289283034E-4 70-71 1.2670097644641519E-5 5.701543940088683E-4 0.0 3.3908222120495415 3.040823434713964E-4 72-73 1.2670097644641519E-5 5.828244916535098E-4 0.0 3.7120028522923816 3.4209263640532097E-4 74-75 1.2670097644641519E-5 5.828244916535098E-4 0.0 4.030725493592161 3.4209263640532097E-4 76-77 1.2670097644641519E-5 5.954945892981513E-4 0.0 4.381585837567574 3.4209263640532097E-4 78-79 1.2670097644641519E-5 5.954945892981513E-4 0.0 4.761245313489258 3.4209263640532097E-4 80-81 1.2670097644641519E-5 6.398399310543967E-4 0.0 5.163425887974292 3.484276852276418E-4 82-83 1.2670097644641519E-5 6.525100286990382E-4 0.0 5.563129458369797 3.6743283169460403E-4 84-85 1.2670097644641519E-5 6.588450775213589E-4 0.0 5.964011347846254 3.6743283169460403E-4 86-87 2.5340195289283037E-5 6.841852728106419E-4 0.0 6.37796244304316 3.6743283169460403E-4 88 2.5340195289283037E-5 6.841852728106419E-4 0.0 6.7047115912008195 3.6743283169460403E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14565 0.0 53.08532 1 GTATCAA 26660 0.0 41.491608 1 CAACGCA 33310 0.0 32.769257 5 ATCAACG 33435 0.0 32.689114 3 TATCAAC 34640 0.0 31.620335 2 TCAACGC 34895 0.0 31.402002 4 AACGCAG 35530 0.0 30.986841 6 ACGCAGA 39845 0.0 27.27838 7 CGCAGAG 40620 0.0 26.698223 8 GTACATG 34390 0.0 25.587315 1 GTACTAG 3035 0.0 25.25876 1 TACATGG 35585 0.0 24.278229 2 GCAGAGT 43905 0.0 24.263393 9 ACATGGG 35450 0.0 23.673363 3 GTGGTAT 7200 0.0 22.53563 1 GAGTACT 24480 0.0 22.47838 12-13 TACCTGG 5090 0.0 21.436064 2 TGGTATC 7510 0.0 21.229248 2 CATGGGG 21750 0.0 20.890999 4 CAGAGTA 43145 0.0 20.841478 10-11 >>END_MODULE