FastQCFastQC Report
Mon 6 Jun 2016
ERR1211307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7892599
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283060.3586397839292228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240760.3050452708923892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139260.17644377979927778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.052.5692061
GTATCAA253750.040.985761
CAACGCA319850.031.751645
ATCAACG321600.031.5121213
TATCAAC335200.030.5693232
TCAACGC334950.030.3547844
AACGCAG342400.030.112626
ACGCAGA387700.026.15867
CGCAGAG396100.025.5209628
GTGGTAT71800.024.7079471
GTACATG339500.024.3300061
TACATGG346750.023.3675422
GTACTAG32150.023.0645731
GCAGAGT434150.022.905649
ACATGGG346250.022.4800133
TGGTATC74950.022.3477482
GAGTACT248800.021.02914212-13
TACCTGG53100.020.1454732
CATGGGG214600.020.0924994
CAGAGTA427850.019.91973910-11
Produced by FastQC (version 0.11.5)