##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7892599 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09605999240554 33.0 31.0 34.0 30.0 34.0 2 32.23061136135258 33.0 31.0 34.0 30.0 34.0 3 32.29206006791932 34.0 31.0 34.0 30.0 34.0 4 35.93653839502045 37.0 35.0 37.0 35.0 37.0 5 35.841894919531576 37.0 35.0 37.0 35.0 37.0 6 35.86227591190177 37.0 35.0 37.0 35.0 37.0 7 35.781925320163865 37.0 35.0 37.0 33.0 37.0 8 35.766377589942174 37.0 35.0 37.0 33.0 37.0 9 37.37823269622592 39.0 37.0 39.0 34.0 39.0 10-11 37.33118507604402 39.0 37.0 39.0 34.0 39.0 12-13 37.26247019517905 39.0 37.0 39.0 34.0 39.0 14-15 38.4864571860296 40.0 38.0 41.0 34.0 41.0 16-17 38.469539058046664 40.0 38.0 41.0 34.0 41.0 18-19 38.49063850830379 40.0 38.0 41.0 34.0 41.0 20-21 38.46513455200245 40.0 38.0 41.0 34.0 41.0 22-23 38.35722922195845 40.0 38.0 41.0 34.0 41.0 24-25 38.26372219087781 40.0 38.0 41.0 34.0 41.0 26-27 38.162442434995114 40.0 38.0 41.0 33.0 41.0 28-29 38.06241701877924 40.0 37.5 41.0 33.0 41.0 30-31 38.06136293000569 40.0 37.0 41.0 33.0 41.0 32-33 37.98848364144688 40.0 37.0 41.0 33.0 41.0 34-35 37.857931462120405 40.0 37.0 41.0 33.0 41.0 36-37 37.707476016455416 40.0 37.0 41.0 33.0 41.0 38-39 37.59438221807544 40.0 37.0 41.0 33.0 41.0 40-41 37.486090956857176 40.0 36.0 41.0 32.0 41.0 42-43 37.37688390098116 40.0 36.0 41.0 32.0 41.0 44-45 37.21103739085186 40.0 36.0 41.0 31.0 41.0 46-47 36.95649601607785 39.0 36.0 41.0 31.0 41.0 48-49 35.37369338034277 39.0 35.0 40.5 24.0 41.0 50-51 36.11776754399913 39.0 35.0 40.0 28.5 41.0 52-53 36.22009993159415 39.0 35.0 40.0 29.0 41.0 54-55 36.05284691392531 38.5 35.0 40.0 29.0 41.0 56-57 35.83773272910482 38.0 35.0 40.0 28.0 41.0 58-59 35.6018784179964 38.0 34.0 40.0 28.0 41.0 60-61 35.34910287473112 37.5 34.0 40.0 28.0 41.0 62-63 35.038056728841795 37.0 34.0 40.0 27.5 41.0 64-65 34.70632246741536 36.5 34.0 39.5 26.5 41.0 66-67 34.36835838232754 36.0 33.0 39.0 26.0 40.5 68-69 34.01059701626802 35.5 33.0 39.0 26.0 40.0 70-71 33.65423176826797 35.0 32.5 38.0 26.0 40.0 72-73 33.27866600089527 35.0 32.0 37.5 25.5 39.5 74-75 32.9127526306607 35.0 32.0 37.0 25.0 39.0 76-77 31.97738216524113 34.0 30.5 36.0 24.5 38.5 78-79 32.41728599159795 35.0 31.5 36.0 25.0 38.0 80-81 32.28785581276839 35.0 32.0 36.0 25.0 37.0 82-83 32.01852824399162 34.0 31.0 35.0 24.5 37.0 84-85 31.735173039958067 34.0 31.0 35.0 24.0 36.5 86-87 31.477713234892587 34.0 31.0 35.0 24.0 36.0 88-89 31.247380425636727 34.0 31.0 35.0 23.0 36.0 90-91 31.02692231038217 34.0 30.5 35.0 22.5 35.0 92-93 30.83296123368234 34.0 30.0 35.0 20.5 35.0 94-95 30.63915992691381 34.0 30.0 35.0 20.0 35.0 96-97 30.452839109145163 34.0 29.5 35.0 20.0 35.0 98-99 30.23255166010588 34.0 29.0 35.0 19.0 35.0 100 27.794835009354966 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.22284945480641838 1101 2 -0.22690920635760037 1101 3 -0.2565776984770096 1101 4 -0.08513059209299456 1101 5 -0.16504160402527646 1101 6 -0.23023440797223316 1101 7 -0.1863913991239201 1101 8 -0.1908429915837786 1101 9 -0.34031077454677927 1101 10-11 -0.27479981142835896 1101 12-13 -0.27340094705642315 1101 14-15 -0.40018879211817904 1101 16-17 -0.4587298287844348 1101 18-19 -0.4744823445965807 1101 20-21 -0.5170614999888485 1101 22-23 -0.5587582186400084 1101 24-25 -0.556667397342089 1101 26-27 -0.6212200007407276 1101 28-29 -0.5616078388720851 1101 30-31 -0.544694087676632 1101 32-33 -0.4804603576566109 1101 34-35 -0.5829408452193974 1101 36-37 -0.5563553409865918 1101 38-39 -0.5169425662697336 1101 40-41 -0.4814130830540151 1101 42-43 -0.5771029897646756 1101 44-45 -0.496570687505411 1101 46-47 -0.5247196416306394 1101 48-49 0.7063116975058037 1101 50-51 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1105 68-69 -0.8213896948653385 1105 70-71 -0.8360681892313906 1105 72-73 -0.7638899289436623 1105 74-75 -0.874923523804874 1105 76-77 -0.8347492781117296 1105 78-79 -0.6449417872130709 1105 80-81 -0.6699556957505166 1105 82-83 -0.6860266127870887 1105 84-85 -0.6699244500937063 1105 86-87 -0.6495550203102951 1105 88-89 -0.6884011980804168 1105 90-91 -0.769454746527007 1105 92-93 -0.6708317743724699 1105 94-95 -0.7311130098042149 1105 96-97 -0.706826548071799 1105 98-99 -0.8069961788842832 1105 100 -0.8197108009613636 1106 1 -0.2955954785094299 1106 2 -0.3127516399264465 1106 3 -0.3411415209938511 1106 4 -0.20613472120943044 1106 5 -0.2693649160445233 1106 6 -0.3931972731500153 1106 7 -0.3234889550705802 1106 8 -0.32934520753079255 1106 9 -0.4854855210919311 1106 10-11 -0.4684854549321855 1106 12-13 -0.4882000495272152 1106 14-15 -0.6383136264012066 1106 16-17 -0.6256634851152114 1106 18-19 -0.6102361190418435 1106 20-21 -0.6290564132905843 1106 22-23 -0.6349281132869322 1106 24-25 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2311 3 0.41483091965392305 2311 4 0.31197191916447053 2311 5 0.3700738181272243 2311 6 0.4231896467072147 2311 7 0.41268003156456956 2311 8 0.19744288783267905 2311 9 0.46471997046807445 2311 10-11 0.40558924611971037 2311 12-13 0.50729775709069 2311 14-15 0.5019425984943524 2311 16-17 0.5310044774441778 2311 18-19 0.6301198411628022 2311 20-21 0.7416738896455897 2311 22-23 0.727045579049431 2311 24-25 0.700352313022826 2311 26-27 0.7603900583676122 2311 28-29 0.7860604436412046 2311 30-31 0.6640113852429863 2311 32-33 0.7270437494412647 2311 34-35 0.8238337888995773 2311 36-37 0.8627190853428672 2311 38-39 0.8844041225413903 2311 40-41 0.8853570417255483 2311 42-43 0.9084521062122803 2311 44-45 0.9518145773283067 2311 46-47 0.9366758216140099 2311 48-49 -5.810738977519016 2311 50-51 -0.8983811088475946 2311 52-53 0.5182686663001945 2311 54-55 0.9392347460448818 2311 56-57 1.0249984951268374 2311 58-59 1.03262593674728 2311 60-61 0.9222144899153832 2311 62-63 0.8239226317974158 2311 64-65 0.8959458310185724 2311 66-67 1.0430482803345953 2311 68-69 1.0900216518725827 2311 70-71 1.1104268308027585 2311 72-73 0.6002787319026552 2311 74-75 0.8389789746626946 2311 76-77 0.9561364190315409 2311 78-79 0.8929061047860856 2311 80-81 0.8957488802424436 2311 82-83 0.9239383620879842 2311 84-85 0.9730700270964583 2311 86-87 0.9923360204263822 2311 88-89 0.9754952615725081 2311 90-91 1.0275774466319412 2311 92-93 1.049875104594964 2311 94-95 1.1105169117214935 2311 96-97 1.123210689120718 2311 98-99 1.1095492425254108 2311 100 1.261532467600091 2312 1 0.066593210831698 2312 2 0.23977630184889165 2312 3 0.2531397483585138 2312 4 -0.002817674058938735 2312 5 0.20787839622217774 2312 6 0.1683136167054755 2312 7 0.3105900543993414 2312 8 0.331172661822265 2312 9 0.2583936107039406 2312 10-11 0.2515818745949332 2312 12-13 0.28353683134716334 2312 14-15 0.32066510418045624 2312 16-17 0.42232004037378346 2312 18-19 0.33389087403063655 2312 20-21 0.3313016597306486 2312 22-23 0.6276959649484084 2312 24-25 0.7001497165320885 2312 26-27 0.6517403185191526 2312 28-29 0.7158017096329772 2312 30-31 0.7748604114436972 2312 32-33 0.7525874678467943 2312 34-35 0.382840696069664 2312 36-37 0.683009023327287 2312 38-39 0.7713892251774226 2312 40-41 0.5573231425162035 2312 42-43 0.5891099199043381 2312 44-45 0.7135866507834692 2312 46-47 0.7728844804033628 2312 48-49 -4.522073216728202 2312 50-51 -0.7888907435251298 2312 52-53 0.4595001937344776 2312 54-55 0.7319145872741544 2312 56-57 0.8607933630807381 2312 58-59 0.8607399336113062 2312 60-61 0.8760366511980422 2312 62-63 0.6672123349228087 2312 64-65 0.8657353480257086 2312 66-67 0.8516501474262199 2312 68-69 0.8078704179970231 2312 70-71 0.8547619624009712 2312 72-73 0.916874344931756 2312 74-75 0.5682650977875454 2312 76-77 0.7828276180847276 2312 78-79 0.4333758270728154 2312 80-81 0.6125367346998516 2312 82-83 0.5851704832564231 2312 84-85 0.6957974813826873 2312 86-87 0.8066777372044633 2312 88-89 0.8541406105169393 2312 90-91 0.6684938503681614 2312 92-93 0.8533370428481213 2312 94-95 0.9462694725881882 2312 96-97 1.0053889680105428 2312 98-99 1.0076529699526944 2312 100 0.6240086523679054 2313 1 0.09170507231780078 2313 2 0.25210597853109107 2313 3 0.3161923713289525 2313 4 0.0921269699563041 2313 5 0.15806044278466658 2313 6 0.2576476419008955 2313 7 0.18035595256012726 2313 8 0.2519395283466821 2313 9 0.4480398721175831 2313 10-11 0.23116097186368734 2313 12-13 0.03340452231406488 2313 14-15 0.12185639543896087 2313 16-17 0.11331687852474914 2313 18-19 0.41659475696391013 2313 20-21 0.39551624420963805 2313 22-23 0.5071767170251391 2313 24-25 0.30925001183199896 2313 26-27 0.4369711074861513 2313 28-29 0.43444761510350816 2313 30-31 0.5854805693330931 2313 32-33 0.5483942072048293 2313 34-35 0.42858331607484246 2313 36-37 -0.21747441928264521 2313 38-39 0.19208560953834564 2313 40-41 0.4301925132651121 2313 42-43 0.4941528543727216 2313 44-45 0.510660174276893 2313 46-47 0.44220631052361625 2313 48-49 -5.420544373686482 2313 50-51 -1.2576556358939825 2313 52-53 0.100226172650558 2313 54-55 0.3452615674327646 2313 56-57 0.38438349524099635 2313 58-59 0.47859556162493533 2313 60-61 0.7435736474330312 2313 62-63 0.8082545127781486 2313 64-65 0.8316959836555213 2313 66-67 0.8998650117753044 2313 68-69 0.8551563470810706 2313 70-71 0.7848036430213767 2313 72-73 0.6476383519342193 2313 74-75 0.7455888406809663 2313 76-77 0.840799291132452 2313 78-79 0.7418919298026339 2313 80-81 -0.21912315604132715 2313 82-83 0.20277254624805963 2313 84-85 0.6230347999969759 2313 86-87 0.7711882485208612 2313 88-89 0.7302736820580478 2313 90-91 0.7918509352314906 2313 92-93 0.8626064766262793 2313 94-95 0.716786180065732 2313 96-97 0.7220076282066046 2313 98-99 0.857442590168688 2313 100 0.9610412583103383 2314 1 0.055403948504640255 2314 2 0.16944083083365058 2314 3 0.17119608834024547 2314 4 0.0705245297031496 2314 5 0.1826254241652734 2314 6 0.2561027421391344 2314 7 0.07783334510831708 2314 8 0.2595865517741558 2314 9 0.37826495639634317 2314 10-11 0.29254803688120745 2314 12-13 0.21802837865997304 2314 14-15 0.24995685629776432 2314 16-17 0.43535477559624525 2314 18-19 0.4856460119656987 2314 20-21 0.5621261257134123 2314 22-23 0.27513304837654573 2314 24-25 0.365876045258716 2314 26-27 0.4436987940342121 2314 28-29 0.5499063073082269 2314 30-31 0.4970848409868083 2314 32-33 0.46689480423960816 2314 34-35 0.36228101409378866 2314 36-37 0.4194445695674105 2314 38-39 0.48382742506615983 2314 40-41 0.24629021699418985 2314 42-43 0.2795478550376984 2314 44-45 0.5034274295576822 2314 46-47 0.6046588359590359 2314 48-49 -6.329147705335686 2314 50-51 -1.5560192022443502 2314 52-53 -0.7503318579008607 2314 54-55 -0.30599651078746604 2314 56-57 -0.010175108312012071 2314 58-59 0.39419492021009006 2314 60-61 0.6755796942171415 2314 62-63 0.5428879598082972 2314 64-65 0.6958034419642871 2314 66-67 0.7602187539762681 2314 68-69 0.7026213932609124 2314 70-71 0.5259777288931886 2314 72-73 0.7419885489701485 2314 74-75 0.7644639442224062 2314 76-77 0.6546685725006292 2314 78-79 0.4874240171692037 2314 80-81 0.5432463871620214 2314 82-83 0.5792531843596898 2314 84-85 0.5775221018045507 2314 86-87 0.5470906627892695 2314 88-89 0.5016242708528758 2314 90-91 0.7428585759755713 2314 92-93 0.8195904282447763 2314 94-95 0.7908283178188604 2314 96-97 0.7707903295938081 2314 98-99 0.8053734418322946 2314 100 -0.6290084000209717 2315 1 0.08849157297484567 2315 2 0.1575649850326144 2315 3 0.26213076225314325 2315 4 0.10621613771957072 2315 5 0.209870342982164 2315 6 0.2898383329296479 2315 7 0.290944861841993 2315 8 0.24206641671140972 2315 9 0.404811626172183 2315 10-11 0.16387817383634484 2315 12-13 0.38995347287436743 2315 14-15 0.2072834149500764 2315 16-17 0.31206870220363925 2315 18-19 0.388990784510284 2315 20-21 0.3374250235265208 2315 22-23 0.3591794042623917 2315 24-25 0.4192913590315115 2315 26-27 0.5146728274253931 2315 28-29 0.5058364001938145 2315 30-31 0.5472806279842075 2315 32-33 0.5728404106586993 2315 34-35 0.5539433203394282 2315 36-37 0.47737048467845256 2315 38-39 0.5748287709799129 2315 40-41 0.6003657219929934 2315 42-43 0.6606289835146555 2315 44-45 0.6653441295307303 2315 46-47 0.5583814172265917 2315 48-49 -4.90585628482599 2315 50-51 -1.0118126872755226 2315 52-53 0.20958465942570115 2315 54-55 0.17076441099002437 2315 56-57 0.498381598575655 2315 58-59 0.637205428135367 2315 60-61 0.5300116323967146 2315 62-63 0.29862082550802427 2315 64-65 0.4431087491654253 2315 66-67 -0.21068855032356026 2315 68-69 0.45978859295888697 2315 70-71 0.44783763761389395 2315 72-73 0.5652400244470002 2315 74-75 0.7338208646328326 2315 76-77 0.6891256093842806 2315 78-79 0.5722458181368424 2315 80-81 0.40019599991181565 2315 82-83 0.3942508233985258 2315 84-85 0.4360088599125298 2315 86-87 0.4713793034012994 2315 88-89 0.558185373105264 2315 90-91 0.6039490267914012 2315 92-93 0.6390624269307921 2315 94-95 0.22382478816144769 2315 96-97 0.38424304496413697 2315 98-99 0.38455952788390846 2315 100 0.4631958156444185 2316 1 0.22332877374384452 2316 2 0.25508023992546214 2316 3 0.20576332610698955 2316 4 -0.02519978318524352 2316 5 0.12926062373814062 2316 6 0.12736044103222355 2316 7 0.2671688690295895 2316 8 0.3178839147483785 2316 9 0.4480211498540001 2316 10-11 0.37682843378933484 2316 12-13 0.3660221943191928 2316 14-15 0.47949876065234065 2316 16-17 0.3968067927628809 2316 18-19 0.08105136691848003 2316 20-21 0.4341087712506422 2316 22-23 0.5741623863992018 2316 24-25 0.5643786003742193 2316 26-27 0.6056494645584181 2316 28-29 0.6859369543551352 2316 30-31 0.5934587424473534 2316 32-33 0.5233735050899 2316 34-35 0.49556816705055695 2316 36-37 0.40929530548528703 2316 38-39 0.5255590347313941 2316 40-41 0.1347685685707276 2316 42-43 0.1106334060440588 2316 44-45 0.3460469699643909 2316 46-47 0.3790378416316784 2316 48-49 -4.909572006133157 2316 50-51 -1.0052176384254992 2316 52-53 0.2158911959733416 2316 54-55 0.5240813486724178 2316 56-57 0.4146112624520839 2316 58-59 0.24183928728991333 2316 60-61 0.39339471640567325 2316 62-63 0.3054848509708421 2316 64-65 0.33482241108798405 2316 66-67 0.5038201112580083 2316 68-69 0.10623109469822367 2316 70-71 -0.25538643122821014 2316 72-73 -0.11459203962252928 2316 74-75 0.17043323512761788 2316 76-77 0.12591581132599217 2316 78-79 0.19482151337375342 2316 80-81 0.12495487315141673 2316 82-83 0.06556993539140521 2316 84-85 0.18091669583266423 2316 86-87 0.22306860056578515 2316 88-89 0.019925339881975646 2316 90-91 0.1597712065504986 2316 92-93 0.2063161929294992 2316 94-95 0.41085642214213536 2316 96-97 0.5078386325800359 2316 98-99 0.49385934802511144 2316 100 -0.2479518946775947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 8.0 11 10.0 12 38.0 13 170.0 14 654.0 15 2054.0 16 4657.0 17 9671.0 18 16751.0 19 24962.0 20 35993.0 21 50689.0 22 63051.0 23 65689.0 24 65229.0 25 71170.0 26 83504.0 27 102684.0 28 126927.0 29 156357.0 30 192669.0 31 238448.0 32 301790.0 33 392068.0 34 545137.0 35 771697.0 36 1199362.0 37 1774365.0 38 1420496.0 39 176277.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64888844783951 20.613583332287742 14.599317412718166 23.13821080715458 2 15.423123345100661 20.75577315807439 40.244554495839964 23.57654900098499 3 18.388144020132298 26.16322067113322 30.305852666127002 25.142782642607482 4 12.64870566995474 17.42949007070097 38.5831341888993 31.33867007044498 5 13.173549739944631 37.919341657639166 34.80010896351623 14.106999638899975 6 30.333162878221565 37.78149800141804 17.883912973627435 14.001426146732962 7 27.25948471200097 32.66524727732168 22.261750126320923 17.81351788435643 8 25.911722650664544 35.47906594858143 20.006918148546333 18.602293252207694 9 26.20941632700954 16.147303612796197 20.957509976834128 36.68577008336013 10-11 24.80010972417863 26.6081576632551 27.922864916042318 20.668867696523954 12-13 25.351264492623937 24.2786194776938 28.275474756868547 22.094641272813718 14-15 22.916631559902193 25.68826890941137 25.992966061461946 25.40213346922449 16-17 21.878524278457732 28.582123979087683 26.95041274879561 22.588938993658974 18-19 21.560507903798964 27.638215912962487 29.30345360342103 21.49782257981752 20-21 22.559874778859225 26.75594611000663 29.241047021658396 21.443132089475743 22-23 22.59443558098142 26.539459360289975 28.936636150225226 21.929468908503384 24-25 22.19983414833778 26.78332664985344 29.22367738367419 21.793161818134593 26-27 22.059028393905855 26.961055820512186 29.005846812165558 21.9740689734164 28-29 21.827132872732577 26.897035464661883 29.330713637224925 21.94511802538062 30-31 22.313108675923477 26.74642495696011 29.152578988297268 21.787887378819146 32-33 22.249836524275153 27.091670394671358 28.7734635420148 21.885029539038687 34-35 22.352679752112458 26.99098446755413 29.002602786232035 21.653732994101375 36-37 22.389729767302843 26.73888594633719 28.82755835297563 22.043825933384333 38-39 22.249214494873648 26.926698163964335 28.995880909774836 21.82820643138718 40-41 22.151838548311858 26.58803098266983 29.34135693278132 21.91877353623699 42-43 22.08783281919688 26.63677469825263 28.95080686014213 22.324585622408357 44-45 22.030087701997576 27.07566262122959 28.80645720663686 22.087792470135977 46-47 22.126403682473114 26.899839615350547 28.455274199207693 22.51848250296864 48-49 22.46867800012506 26.99018597111748 28.2701687833394 22.270967245418063 50-51 22.298646725542152 26.978842335098584 28.17272465762625 22.549786281733013 52-53 22.121884491277356 27.279930990845795 28.057557558001538 22.54062695987531 54-55 22.16775537359438 27.20895323146163 28.00547703964892 22.61781435529507 56-57 22.35681136421405 27.160072944735695 27.823915908789683 22.659199782260572 58-59 22.02905556581566 27.108783985195036 28.196082145756797 22.666078303232503 60-61 22.237608000497705 27.390506257500274 27.91477645865689 22.45710928334513 62-63 22.207494939973742 27.179596767842696 27.954805398298998 22.658102893884564 64-65 21.887860626623112 27.012722321919746 28.032860095010943 23.0665569564462 66-67 22.08251120405605 27.01522983594675 27.832742705648283 23.069516254348912 68-69 21.89370447825773 27.07141393324456 27.922164910105707 23.112716678392 70-71 22.041823811812872 26.801587067229587 28.024046296515735 23.13254282444181 72-73 22.20939438129584 27.023657735067687 27.773554165389342 22.99339371824713 74-75 22.10085182014316 27.279475302765867 27.664663063505074 22.9550098135859 76-77 22.021393419647385 27.136731558493626 27.70190351837143 23.13997150348755 78-79 22.001113158554137 27.0524328089704 27.66194572132453 23.28450831115094 80-81 21.864432646706863 27.374703661931104 27.558380840657755 23.20248285070428 82-83 22.251620240234445 27.027341254979486 27.834070064884376 22.886968439901693 84-85 22.210293324367544 27.160896471564662 27.445667137112885 23.183143066954912 86-87 21.798257678394446 27.383310689856476 27.73330717626814 23.085124455480933 88-89 22.005825185253908 26.948487458792243 27.71471839308486 23.330968962868994 90-91 22.052300351306197 26.906425241631915 27.777318392475863 23.26395601458602 92-93 22.11767899159845 27.068912765677993 27.583385250492025 23.23002299223153 94-95 21.70737438584903 26.80681281756066 27.848982294877487 23.636830501712822 96-97 21.886887577807656 26.79891035545602 27.71977643775131 23.594425628985014 98-99 21.653861031321192 26.975444416741933 27.624018985266524 23.74667556667035 100 21.770005632568925 26.740354796074133 27.579004257127025 23.910635314229918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3023.0 1 2073.0 2 1822.0 3 2416.5 4 1766.5 5 1374.5 6 2149.0 7 4081.0 8 6599.0 9 7950.5 10 6949.0 11 5471.5 12 5381.5 13 5963.0 14 6721.0 15 7554.5 16 8327.0 17 9193.5 18 10118.0 19 11023.0 20 12190.5 21 13453.0 22 14923.5 23 17394.0 24 20234.0 25 23850.0 26 30416.5 27 38278.0 28 45259.0 29 56475.0 30 73862.5 31 91017.5 32 113554.5 33 140898.5 34 167765.0 35 193106.5 36 221516.5 37 252626.0 38 266076.0 39 266066.5 40 260846.0 41 253830.0 42 254164.5 43 262158.5 44 273669.0 45 291894.0 46 303749.5 47 306920.5 48 313689.5 49 315710.0 50 308691.5 51 298206.5 52 287266.5 53 275137.0 54 263144.5 55 243496.0 56 219032.0 57 190591.0 58 163343.5 59 143371.0 60 119436.0 61 93978.0 62 73924.0 63 57393.5 64 42102.5 65 29414.5 66 20899.0 67 15443.0 68 11451.5 69 8397.5 70 6415.5 71 4855.5 72 3597.5 73 2789.5 74 2147.5 75 1491.0 76 1010.0 77 604.0 78 369.5 79 222.5 80 120.5 81 72.5 82 51.5 83 35.0 84 18.5 85 9.0 86 7.0 87 6.0 88 5.5 89 5.5 90 3.0 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02947064712143617 2 0.0015837622055801897 3 1.0136078115713214E-4 4 0.022299371854569074 5 6.208347845874344E-4 6 3.801029293392456E-5 7 3.801029293392456E-5 8 0.003978410660417437 9 0.01035146977567212 10-11 0.0010326129580382837 12-13 8.235563469016987E-5 14-15 1.0136078115713214E-4 16-17 3.2942253876067947E-4 18-19 2.5340195289283034E-5 20-21 2.5340195289283034E-5 22-23 2.914122458267549E-4 24-25 5.068039057856607E-5 26-27 9.502573233481138E-5 28-29 0.0010199428603936422 30-31 0.002679725651841681 32-33 0.009559588672882025 34-35 0.018244940608283786 36-37 0.02125408879888615 38-39 0.021000686845993318 40-41 0.004820972153786097 42-43 0.005080709155501249 44-45 0.008913413693005308 46-47 0.011985912371830876 48-49 1.2209096141841236 50-51 0.017345363675514237 52-53 0.018650383732912314 54-55 0.00943288769643561 56-57 0.007564048293850986 58-59 0.008254568615483949 60-61 0.004941338081410192 62-63 0.007532373049739383 64-65 0.01910650724811941 66-67 0.028691436116290717 68-69 0.028837142239204094 70-71 0.02622710212440794 72-73 0.022502093416883338 74-75 0.009293516622344553 76-77 0.014025798092618162 78-79 0.008127867639037533 80-81 0.0027810864329988133 82-83 0.01393077236028335 84-85 0.023610726960789467 86-87 0.02287586129740026 88-89 0.03230241394501355 90-91 0.020519223135496936 92-93 0.012929834646356667 94-95 0.006392064261721646 96-97 0.003807364342214776 98-99 0.015413173784706408 100 0.035108840573301645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7892599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.85444553989354 #Duplication Level Percentage of deduplicated Percentage of total 1 72.750527785667 39.90689864417382 2 15.669984411589772 17.191366130330636 3 5.416322718683101 8.913281387954708 4 2.3783006209345454 5.21841447554196 5 1.175591752880107 3.2243216892754902 6 0.7041173768198491 2.3174380980274263 7 0.40666696845880523 1.561524375193799 8 0.28767956170945036 1.262440228058519 9 0.2044002493939249 1.0091036113847738 >10 0.9056794276019952 8.536919403268364 >50 0.055295750755232166 2.0943473520148377 >100 0.03930830290391972 4.386167765505608 >500 0.004240442611653685 1.5338761771527185 >1k 0.0017500135628631785 1.5089651883183155 >5k 3.365410697591559E-5 0.11083848579337893 >10k+ 1.0096232092774677E-4 1.2240969880058912 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28306 0.3586397839292228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24076 0.3050452708923892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13926 0.17644377979927778 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.801029293392455E-5 0.0 0.0 0.0 6 0.0 3.801029293392455E-5 0.0 0.0 0.0 7 0.0 3.801029293392455E-5 0.0 0.0 0.0 8 0.0 3.801029293392455E-5 0.0 0.0 0.0 9 0.0 3.801029293392455E-5 0.0 0.0 1.2670097644641519E-5 10-11 0.0 3.801029293392455E-5 0.0 6.335048822320759E-6 1.2670097644641519E-5 12-13 0.0 5.7015439400886826E-5 0.0 1.2670097644641519E-5 1.2670097644641519E-5 14-15 0.0 8.869068351249063E-5 0.0 1.2670097644641519E-5 4.4345341756245316E-5 16-17 0.0 1.0136078115713215E-4 0.0 1.2670097644641519E-5 5.0680390578566074E-5 18-19 0.0 1.1403087880177367E-4 0.0 3.167524411160379E-5 5.0680390578566074E-5 20-21 0.0 1.1403087880177367E-4 0.0 1.2036592762409442E-4 5.0680390578566074E-5 22-23 0.0 1.1403087880177367E-4 0.0 4.1811322227317004E-4 5.701543940088683E-5 24-25 0.0 1.267009764464152E-4 0.0 0.001209994325063265 7.60205858678491E-5 26-27 0.0 1.393710740910567E-4 0.0 0.0028697771165113036 7.60205858678491E-5 28-29 0.0 1.393710740910567E-4 0.0 0.00660112087285823 7.60205858678491E-5 30-31 0.0 1.520411717356982E-4 0.0 0.01794719331363471 8.235563469016987E-5 32-33 0.0 1.520411717356982E-4 0.0 0.04413628514510873 1.1403087880177367E-4 34-35 0.0 1.520411717356982E-4 0.0 0.0856625301754213 1.393710740910567E-4 36-37 0.0 1.8371641584730202E-4 0.0 0.1484998794440209 1.393710740910567E-4 38-39 0.0 2.0905661113658505E-4 0.0 0.23590454804558042 1.520411717356982E-4 40-41 0.0 2.2172670878122658E-4 0.0 0.32991033751999815 1.6471126938033973E-4 42-43 0.0 2.5340195289283034E-4 0.0 0.4237894260179695 1.6471126938033973E-4 44-45 0.0 2.5340195289283034E-4 0.0 0.5411145302073499 1.7738136702498126E-4 46-47 0.0 2.5340195289283034E-4 0.0 0.6818666449416726 1.9005146466962277E-4 48-49 0.0 2.5973700171515114E-4 0.0 0.8321466731047656 1.9005146466962277E-4 50-51 0.0 2.660720505374719E-4 0.0 0.9910930480567934 1.9005146466962277E-4 52-53 0.0 2.9774729464907565E-4 0.0 1.1443568842151997 2.0272156231426427E-4 54-55 0.0 3.864379781615663E-4 0.0 1.3188241287819134 2.153916599589058E-4 56-57 0.0 4.054431246285286E-4 0.0 1.5230027523253113 2.153916599589058E-4 58-59 0.0 4.181132222731701E-4 0.0 1.751108855270615 2.153916599589058E-4 60-61 0.0 4.307833199178116E-4 0.0 1.9869310476815052 2.153916599589058E-4 62-63 6.335048822320759E-6 4.434534175624531E-4 0.0 2.217286092958733 2.2806175760354733E-4 64-65 1.2670097644641519E-5 4.434534175624531E-4 0.0 2.461008091251057 2.2806175760354733E-4 66-67 1.2670097644641519E-5 5.068039057856607E-4 0.0 2.7321481808463854 2.4073185524818883E-4 68-69 1.2670097644641519E-5 5.384791498972645E-4 0.0 3.031872010728025 2.5340195289283034E-4 70-71 1.2670097644641519E-5 5.701543940088683E-4 0.0 3.353838197024833 2.660720505374719E-4 72-73 1.2670097644641519E-5 5.954945892981513E-4 0.0 3.674759100265958 3.1675244111603796E-4 74-75 1.2670097644641519E-5 5.954945892981513E-4 0.0 3.9936337827374735 3.1675244111603796E-4 76-77 1.2670097644641519E-5 6.144997357651137E-4 0.0 4.34453213700582 3.42092636405321E-4 78-79 1.2670097644641519E-5 6.208347845874344E-4 0.0 4.7237734997052305 3.6743283169460403E-4 80-81 1.2670097644641519E-5 6.398399310543967E-4 0.0 5.12466172423051 3.8010292933924553E-4 82-83 1.2670097644641519E-5 6.461749798767174E-4 0.0 5.524194248307813 4.054431246285286E-4 84-85 1.2670097644641519E-5 6.461749798767174E-4 0.0 5.925057132637804 4.054431246285286E-4 86-87 1.2670097644641519E-5 6.461749798767174E-4 0.0 6.3400408407927475 4.054431246285286E-4 88 1.2670097644641519E-5 6.461749798767174E-4 0.0 6.667499514418508 4.054431246285286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14070 0.0 52.569206 1 GTATCAA 25375 0.0 40.98576 1 CAACGCA 31985 0.0 31.75164 5 ATCAACG 32160 0.0 31.512121 3 TATCAAC 33520 0.0 30.569323 2 TCAACGC 33495 0.0 30.354784 4 AACGCAG 34240 0.0 30.11262 6 ACGCAGA 38770 0.0 26.1586 7 CGCAGAG 39610 0.0 25.520962 8 GTGGTAT 7180 0.0 24.707947 1 GTACATG 33950 0.0 24.330006 1 TACATGG 34675 0.0 23.367542 2 GTACTAG 3215 0.0 23.064573 1 GCAGAGT 43415 0.0 22.90564 9 ACATGGG 34625 0.0 22.480013 3 TGGTATC 7495 0.0 22.347748 2 GAGTACT 24880 0.0 21.029142 12-13 TACCTGG 5310 0.0 20.145473 2 CATGGGG 21460 0.0 20.092499 4 CAGAGTA 42785 0.0 19.919739 10-11 >>END_MODULE