FastQCFastQC Report
Mon 6 Jun 2016
ERR1211306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12177296
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT792290.6506288423965386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596510.4898542336492436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349810.28726410198126084No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC167920.13789596639516688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134100.11012296982844139No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG130630.10727340453907008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA309050.055.8193741
GTATCAA605700.043.591241
TACCTGG29400.035.191692
TCAAGAC13500.035.1516153
CAACGCA739250.034.6822975
ATCAACG745100.034.42993
ACCTGGG29900.033.7850843
GTACTAG95000.033.5652961
TATCAAC787600.033.139962
GCATCGT90750.032.4634488
TAGGCAT114550.032.2793545
TATTCTC76950.032.1160665
AACGCAG815750.032.0643046
TCTCTGC80850.030.6851068
TCAACGC847000.030.5030844
CCTCAGG67150.030.0452632
CGTTAAC25650.029.3370881
TACCCCG6300.029.0850145
ACGCAGA883450.028.9969947
CGCAGAG886000.028.8813658
Produced by FastQC (version 0.11.5)