FastQCFastQC Report
Mon 6 Jun 2016
ERR1211306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12177296
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT683180.5610276698538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552150.45342578516609927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330110.271086454661199No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC163160.13398705262646157No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG131690.10814387693294143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126860.10417747913822575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA311900.054.560311
GTATCAA599300.042.624421
TACCCCG5800.038.8202445
ACCTGGG28300.037.635423
TACCTGG29900.035.776942
CAACGCA740950.033.6733325
ATCAACG745400.033.512443
TATTCTC78550.033.1429985
GTACTAG99700.032.668471
TAGGCAT115400.032.2745135
TATCAAC796700.031.8891662
TCTCTGC82600.031.6879278
AACGCAG814400.031.2407596
CATGGGT43100.030.5904884
TATCCGC3400.030.3674411
TCAAGAC17450.029.8459193
CCTCAGG68200.029.7881182
TCAACGC852000.029.5452384
ATGGGTA31050.029.0057855
CCCGGCG7950.028.9113668
Produced by FastQC (version 0.11.5)