##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211306_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12177296 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14564818002289 33.0 31.0 34.0 30.0 34.0 2 32.286154906639375 34.0 31.0 34.0 30.0 34.0 3 32.334250559401696 34.0 31.0 34.0 30.0 34.0 4 35.97318361974612 37.0 35.0 37.0 35.0 37.0 5 35.89660955929789 37.0 35.0 37.0 35.0 37.0 6 35.92019788301114 37.0 35.0 37.0 35.0 37.0 7 35.84372277720768 37.0 35.0 37.0 35.0 37.0 8 35.83404378114813 37.0 35.0 37.0 35.0 37.0 9 37.434330084445676 39.0 37.0 39.0 34.0 39.0 10-11 37.3926334713388 39.0 37.0 39.0 34.0 39.0 12-13 37.321743349262434 39.0 37.0 39.0 34.0 39.0 14-15 38.5085040225679 40.0 38.0 41.0 34.0 41.0 16-17 38.517131225191534 40.0 38.0 41.0 34.0 41.0 18-19 38.58426168666673 40.0 38.0 41.0 34.0 41.0 20-21 38.57653788657186 40.0 38.0 41.0 34.0 41.0 22-23 38.457638091412086 40.0 38.0 41.0 34.0 41.0 24-25 38.364581677245916 40.0 38.0 41.0 34.0 41.0 26-27 38.23461403089816 40.0 38.0 41.0 34.0 41.0 28-29 38.162899095168584 40.0 37.5 41.0 33.5 41.0 30-31 38.1276960418799 40.0 37.0 41.0 34.0 41.0 32-33 38.019279156883435 40.0 37.0 41.0 33.0 41.0 34-35 37.88714481441528 40.0 37.0 41.0 33.0 41.0 36-37 37.741745950825205 40.0 36.5 41.0 33.0 41.0 38-39 37.61205299600174 40.0 36.0 41.0 33.0 41.0 40-41 37.48786684662999 40.0 36.0 41.0 32.5 41.0 42-43 37.36056752664959 40.0 36.0 41.0 32.0 41.0 44-45 37.13074626748008 39.5 36.0 41.0 31.0 41.0 46-47 36.80161691889562 39.0 35.0 41.0 30.5 41.0 48-49 35.158205154904664 39.0 35.0 40.5 23.0 41.0 50-51 35.847913444823874 38.5 35.0 40.0 28.0 41.0 52-53 35.93430844581589 39.0 35.0 40.0 28.5 41.0 54-55 35.76280616813453 38.0 35.0 40.0 28.0 41.0 56-57 35.52161300833946 38.0 34.5 40.0 28.0 41.0 58-59 35.263533217883506 38.0 34.0 40.0 27.0 41.0 60-61 35.024718500724624 37.0 34.0 40.0 27.0 41.0 62-63 34.7108128520486 37.0 34.0 40.0 26.0 41.0 64-65 34.3987577373499 36.0 33.5 39.0 26.0 41.0 66-67 34.09184883901976 36.0 33.0 39.0 26.0 40.5 68-69 33.77999738201322 35.0 33.0 39.0 25.5 40.0 70-71 33.507233543473035 35.0 33.0 38.0 25.0 40.0 72-73 33.17798910365651 35.0 32.0 38.0 25.0 40.0 74-75 32.83556094883461 35.0 32.0 37.0 24.0 39.0 76-77 31.89434830195472 34.0 30.5 36.0 23.5 39.0 78-79 32.34090371951211 35.0 32.0 36.0 24.0 39.0 80-81 32.23369539510249 35.0 32.0 36.0 24.0 37.5 82-83 31.975036412024473 35.0 31.5 35.5 24.0 37.0 84-85 31.682237419538787 34.0 31.0 35.0 23.0 37.0 86-87 31.43681515995012 34.0 31.0 35.0 23.0 36.0 88-89 31.213283022766305 34.0 31.0 35.0 21.5 36.0 90-91 30.994530641285223 34.0 31.0 35.0 20.0 36.0 92-93 30.793939147081588 34.0 30.5 35.0 20.0 35.0 94-95 30.614493398205973 34.0 30.0 35.0 20.0 35.0 96-97 30.441001146724197 34.0 30.0 35.0 19.0 35.0 98-99 30.241431759563042 34.0 29.0 35.0 19.0 35.0 100 27.78415676189525 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2979622989766497 1101 2 -0.23639914400304463 1101 3 -0.23758847357648705 1101 4 -0.16374408793419093 1101 5 -0.16686824195998184 1101 6 -0.19177697369701718 1101 7 -0.2050699264492053 1101 8 -0.1755478578711802 1101 9 -0.2635557986181567 1101 10-11 -0.274519375994835 1101 12-13 -0.3667120091946501 1101 14-15 -0.41357722932864505 1101 16-17 -0.40542466220314566 1101 18-19 -0.4414455152126493 1101 20-21 -0.4691405644875317 1101 22-23 -0.4947049526190668 1101 24-25 -0.49027556406350215 1101 26-27 -0.5447901749949153 1101 28-29 -0.4975390624914553 1101 30-31 -0.46604993551052587 1101 32-33 -0.4442525270809412 1101 34-35 -0.4842025908469765 1101 36-37 -0.461516821668603 1101 38-39 -0.437883877665854 1101 40-41 -0.4405759216767038 1101 42-43 -0.4523651957625887 1101 44-45 -0.3224061874382542 1101 46-47 -0.37335125330194074 1101 48-49 0.8604110103488765 1101 50-51 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0.8793680026906081 2310 90-91 0.9081771303951065 2310 92-93 0.8186529818331074 2310 94-95 0.888315588482822 2310 96-97 1.0032625743810613 2310 98-99 1.0728025590447317 2310 100 1.282429423261732 2311 1 0.35761895217806483 2311 2 0.38777206314435375 2311 3 0.3922234734887482 2311 4 0.2867897495207572 2311 5 0.3450688763449463 2311 6 0.3626556221054571 2311 7 0.36545179832284447 2311 8 0.21725781093776675 2311 9 0.49196684425943715 2311 10-11 0.39170156092156105 2311 12-13 0.48117590846887737 2311 14-15 0.4587144705360302 2311 16-17 0.45959924926197715 2311 18-19 0.6111279033650376 2311 20-21 0.6352254042777901 2311 22-23 0.6505056868718597 2311 24-25 0.620893244575285 2311 26-27 0.6645885860320959 2311 28-29 0.7024707711754274 2311 30-31 0.5924490190842491 2311 32-33 0.6333463534532342 2311 34-35 0.7254305773765708 2311 36-37 0.7443145684154686 2311 38-39 0.7964689690263285 2311 40-41 0.7186753212445822 2311 42-43 0.7367287384223147 2311 44-45 0.8040562519588903 2311 46-47 0.8237480010410181 2311 48-49 -6.376789244503961 2311 50-51 -1.136992486428312 2311 52-53 0.3341698926301362 2311 54-55 0.7442045968714623 2311 56-57 0.8278892172856658 2311 58-59 0.8272629222296572 2311 60-61 0.7287696121884224 2311 62-63 0.6134849148304724 2311 64-65 0.6989493984712212 2311 66-67 0.8230020723844618 2311 68-69 0.8391220555867207 2311 70-71 0.8268851258687491 2311 72-73 0.33759468774015744 2311 74-75 0.6374411551957095 2311 76-77 0.7868683708446191 2311 78-79 0.7146188144297483 2311 80-81 0.7289514375548052 2311 82-83 0.7478456717036615 2311 84-85 0.7358040241341506 2311 86-87 0.8095767373115592 2311 88-89 0.8215960135525116 2311 90-91 0.9307988096429831 2311 92-93 0.8971084746971947 2311 94-95 0.9222445798182797 2311 96-97 0.9984248117502723 2311 98-99 0.9984734927439511 2311 100 1.200163400156427 2312 1 0.1111332984367337 2312 2 0.2282323806351414 2312 3 0.2098299879568657 2312 4 -0.01097315834464041 2312 5 0.1842536789231417 2312 6 0.12442527431442585 2312 7 0.2503464692924027 2312 8 0.29718592676465505 2312 9 0.24454929379947288 2312 10-11 0.2682517953079895 2312 12-13 0.26709316722858745 2312 14-15 0.3015156622534363 2312 16-17 0.4261906712136465 2312 18-19 0.29527598063141625 2312 20-21 0.35115743673719635 2312 22-23 0.60211371760753 2312 24-25 0.6887988369702214 2312 26-27 0.5900224122418507 2312 28-29 0.697582896103512 2312 30-31 0.7275155360655319 2312 32-33 0.7246920682776974 2312 34-35 0.30923017899694827 2312 36-37 0.6254843314656782 2312 38-39 0.7534178687572677 2312 40-41 0.5105459172241567 2312 42-43 0.5356210764015827 2312 44-45 0.6556010524960172 2312 46-47 0.7112492374210362 2312 48-49 -4.952271765998201 2312 50-51 -0.9701637886753929 2312 52-53 0.41117860910662785 2312 54-55 0.6986778704013759 2312 56-57 0.7031123167731366 2312 58-59 0.7079787078424502 2312 60-61 0.7279870223977056 2312 62-63 0.544570629879793 2312 64-65 0.7389905290822938 2312 66-67 0.7432543061426742 2312 68-69 0.6220298641315409 2312 70-71 0.6623016272540738 2312 72-73 0.7722824320959418 2312 74-75 0.4825265280137714 2312 76-77 0.6657332673575276 2312 78-79 0.35667313030991465 2312 80-81 0.5628255093838348 2312 82-83 0.5659662133394683 2312 84-85 0.6245169208417956 2312 86-87 0.6648698286172419 2312 88-89 0.7396268502676371 2312 90-91 0.5218118963180878 2312 92-93 0.6621080055636206 2312 94-95 0.7650181303078654 2312 96-97 0.844730883733039 2312 98-99 0.8801328111278828 2312 100 0.49137695336630927 2313 1 0.10630843859735961 2313 2 0.2427419177077894 2313 3 0.3168163553414587 2313 4 0.08550348913939132 2313 5 0.12820515424573387 2313 6 0.23932495039041868 2313 7 0.16587572917063653 2313 8 0.2182274315223367 2313 9 0.4495601687004154 2313 10-11 0.19122145122693013 2313 12-13 -0.008543119738909866 2313 14-15 0.12287452628504525 2313 16-17 0.0974403615226862 2313 18-19 0.43929168465481183 2313 20-21 0.38355863105941523 2313 22-23 0.48100117480549187 2313 24-25 0.32030510086703146 2313 26-27 0.4319411666638331 2313 28-29 0.4262273743010354 2313 30-31 0.5539626311017756 2313 32-33 0.4805665823610994 2313 34-35 0.3882831638795352 2313 36-37 -0.2914138154675925 2313 38-39 0.17118927078077206 2313 40-41 0.38154434353042177 2313 42-43 0.4761118931619279 2313 44-45 0.47207467569229067 2313 46-47 0.3525957174783514 2313 48-49 -5.373053519715295 2313 50-51 -1.377287111336564 2313 52-53 0.056141482754256344 2313 54-55 0.2395328660266003 2313 56-57 0.21246323296166736 2313 58-59 0.322959827692479 2313 60-61 0.6198174960931553 2313 62-63 0.6980572380013825 2313 64-65 0.7514584448565884 2313 66-67 0.7797886481443967 2313 68-69 0.8242042178297524 2313 70-71 0.7854030763530844 2313 72-73 0.5573984941442447 2313 74-75 0.6631844294993883 2313 76-77 0.6788958600267243 2313 78-79 0.6050869519159647 2313 80-81 -0.2713156484472208 2313 82-83 0.1518883886476594 2313 84-85 0.5269210071869175 2313 86-87 0.6306448087791949 2313 88-89 0.6215227755590398 2313 90-91 0.6830048106354347 2313 92-93 0.7218610427746803 2313 94-95 0.679868372975168 2313 96-97 0.7087323779801373 2313 98-99 0.8732448414668319 2313 100 0.8838281455579953 2314 1 0.06229533922803654 2314 2 0.18623930515568787 2314 3 0.2141266033312661 2314 4 0.0712455822634297 2314 5 0.18637649065232154 2314 6 0.25173460227590283 2314 7 0.0714749771849057 2314 8 0.20666498061498828 2314 9 0.38126626097876226 2314 10-11 0.25857665064226154 2314 12-13 0.17724903172012318 2314 14-15 0.2320109908789192 2314 16-17 0.416525302743203 2314 18-19 0.48928572151463356 2314 20-21 0.5554077471800554 2314 22-23 0.28803408107000195 2314 24-25 0.4037914518755983 2314 26-27 0.45683931509287845 2314 28-29 0.5699046926813551 2314 30-31 0.46485789329455685 2314 32-33 0.42352015846964974 2314 34-35 0.31538540180277863 2314 36-37 0.4059949137504404 2314 38-39 0.4743599622216479 2314 40-41 0.21968713241391669 2314 42-43 0.28386403859893505 2314 44-45 0.4763921219580851 2314 46-47 0.580421018117427 2314 48-49 -6.604861054949119 2314 50-51 -1.7093115057586346 2314 52-53 -0.8613393820869533 2314 54-55 -0.4343220915118806 2314 56-57 -0.17024048664243452 2314 58-59 0.3163889136433866 2314 60-61 0.48747001666856704 2314 62-63 0.3303235234739077 2314 64-65 0.49363185889158245 2314 66-67 0.5456533006287927 2314 68-69 0.6011027129254316 2314 70-71 0.29567619111300303 2314 72-73 0.4981168750282663 2314 74-75 0.5589869623391408 2314 76-77 0.5273475126244769 2314 78-79 0.32418813584646244 2314 80-81 0.40842105444099985 2314 82-83 0.43613735674170684 2314 84-85 0.4547693137589697 2314 86-87 0.5209615490711172 2314 88-89 0.44924068137123285 2314 90-91 0.6048503476960967 2314 92-93 0.6279547375576975 2314 94-95 0.677763354114969 2314 96-97 0.6650612343969335 2314 98-99 0.7556734487007013 2314 100 -0.6433607359934506 2315 1 0.13958592467097475 2315 2 0.2063281310130307 2315 3 0.27620431697408065 2315 4 0.12853269350091523 2315 5 0.22706353444119287 2315 6 0.28355484691671506 2315 7 0.3457790913658272 2315 8 0.23976690714133042 2315 9 0.4038296486513602 2315 10-11 0.19736750763760824 2315 12-13 0.3914586148987311 2315 14-15 0.21094021624058712 2315 16-17 0.35620427567369717 2315 18-19 0.4086691683983048 2315 20-21 0.3566890165711456 2315 22-23 0.4137105912159811 2315 24-25 0.4381873483809784 2315 26-27 0.5157875934567713 2315 28-29 0.5739885610495179 2315 30-31 0.5186869904514069 2315 32-33 0.5926804246619426 2315 34-35 0.6355016392644899 2315 36-37 0.5600856788713315 2315 38-39 0.6449570197716312 2315 40-41 0.5947181755113391 2315 42-43 0.6460451476337781 2315 44-45 0.6274500795848041 2315 46-47 0.5431181441954678 2315 48-49 -4.828295054529068 2315 50-51 -0.9756183455639942 2315 52-53 0.1965048086652743 2315 54-55 0.11570141681572466 2315 56-57 0.4694903642094417 2315 58-59 0.6011506869284275 2315 60-61 0.46401849378538174 2315 62-63 0.19211816955831296 2315 64-65 0.41231011377335136 2315 66-67 -0.25394192667953774 2315 68-69 0.459818107341043 2315 70-71 0.3983229346583528 2315 72-73 0.5108745953820275 2315 74-75 0.6789782679588114 2315 76-77 0.6845314366446935 2315 78-79 0.5610077436267105 2315 80-81 0.4261776097871177 2315 82-83 0.4441327903126826 2315 84-85 0.47752296930777405 2315 86-87 0.49138758720010145 2315 88-89 0.4683865815774979 2315 90-91 0.5441877116269005 2315 92-93 0.6185960002471056 2315 94-95 0.24203799576139318 2315 96-97 0.3836280116943449 2315 98-99 0.33234069530113075 2315 100 0.32318025993927435 2316 1 0.17054146809702786 2316 2 0.1814218387809916 2316 3 0.12181466531285423 2316 4 -0.08797398198947093 2316 5 0.0964642078213842 2316 6 0.06946973431798398 2316 7 0.17948475280238085 2316 8 0.2493574013957982 2316 9 0.40852317893896384 2316 10-11 0.37223839869648145 2316 12-13 0.31961861212506193 2316 14-15 0.4088656281969989 2316 16-17 0.34871813895659187 2316 18-19 0.010910616792379813 2316 20-21 0.3753858539570558 2316 22-23 0.5012391562137068 2316 24-25 0.501217058783098 2316 26-27 0.5295889036798656 2316 28-29 0.5501259785890653 2316 30-31 0.45870292199152374 2316 32-33 0.43311745878316543 2316 34-35 0.4183785945844676 2316 36-37 0.2865390278976534 2316 38-39 0.38634452989369805 2316 40-41 0.013037652359031426 2316 42-43 -0.03199795496712454 2316 44-45 0.2630818124066323 2316 46-47 0.2521002003199371 2316 48-49 -5.322154785995032 2316 50-51 -1.1846162707201842 2316 52-53 0.11226912946904122 2316 54-55 0.3569307575237133 2316 56-57 0.298496614830448 2316 58-59 0.20792325737720319 2316 60-61 0.27986500916227186 2316 62-63 0.17388727883992772 2316 64-65 0.172558777846767 2316 66-67 0.2767957656519897 2316 68-69 0.018272804040194046 2316 70-71 -0.3493390000217076 2316 72-73 -0.24260950632679368 2316 74-75 -0.0581271612072527 2316 76-77 -0.02914185384336676 2316 78-79 0.060752113472993585 2316 80-81 -0.017418536271883056 2316 82-83 -0.04644073328129039 2316 84-85 0.08810957808858433 2316 86-87 0.14650823884593933 2316 88-89 -0.030909473310114066 2316 90-91 -0.0338898920462114 2316 92-93 0.007629050670590942 2316 94-95 0.25015107032476536 2316 96-97 0.3058049741499538 2316 98-99 0.2961505389553203 2316 100 -0.455276365237296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 14.0 11 21.0 12 68.0 13 245.0 14 1073.0 15 3248.0 16 8307.0 17 18511.0 18 33456.0 19 50858.0 20 75991.0 21 113184.0 22 136946.0 23 122881.0 24 106487.0 25 105756.0 26 119817.0 27 146354.0 28 179848.0 29 221528.0 30 273121.0 31 339476.0 32 433865.0 33 571489.0 34 830023.0 35 1161350.0 36 1826689.0 37 2657890.0 38 2269862.0 39 368920.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.614489612583846 23.870241578974 14.934872054029153 21.580396754413 2 14.346385849630606 21.63280416528189 42.26114410900713 21.759665876080383 3 16.882266140233043 30.246753648821777 30.59724222644995 22.27373798449523 4 11.052525272603898 18.8316845524964 42.344325762305644 27.771464412594053 5 11.224130895568193 40.40019318039694 35.707126561491584 12.668549362543285 6 26.152863239686337 41.77900008951089 18.81841526316611 13.249721407636676 7 25.11568662134625 34.00248856601475 24.804878653664765 16.076946158974234 8 25.567186443172297 35.5842336540423 20.70576668430681 18.142813218478594 9 26.518947972023057 15.527102192455896 23.170975489047084 34.782974346473964 10-11 23.911052046100053 27.88990065504568 28.16268169457491 20.036365604279354 12-13 24.681731216627416 26.123214154615404 28.83134222190396 20.363712406853217 14-15 22.735959357106154 26.945285206284737 26.69598081509565 23.62277462151346 16-17 19.718531757531853 29.772404334540802 28.402822048618027 22.10624185930932 18-19 19.238820944803617 28.883624992994132 31.826902408725093 20.050651653477157 20-21 20.91077362482688 28.00035886488655 31.212248871567024 19.876618638719552 22-23 21.448216344732142 27.753456049956686 30.718513321829388 20.079814283481788 24-25 20.68004117828496 28.24083548296288 31.509780663878306 19.569342674873855 26-27 20.283933558098806 28.508985027902977 30.908637232821718 20.298444181176503 28-29 19.665753112598075 28.070914526790773 31.77980279730921 20.48352956330194 30-31 20.531849672088597 27.92673741675234 31.35276782401173 20.188645087147332 32-33 20.876948190992124 28.70135191908796 30.460103700389997 19.96159618952992 34-35 20.677022185257655 28.24932472401282 31.36942175545966 19.704231335269867 36-37 20.63150402906363 27.793643603335287 30.914205852892053 20.66064651470903 38-39 20.654890652716887 28.002949061783312 31.581466825580662 19.76069345991914 40-41 20.033954044807924 27.175767923344097 32.575105718868805 20.215172312979178 42-43 20.189117401483113 27.27415847470273 31.383796340362046 21.152927783452107 44-45 20.01284312055964 28.41440638736233 30.996413198454015 20.576337293624018 46-47 20.374608896078385 27.983621106536642 30.378524863105415 21.26324513427956 48-49 21.281774848936603 27.904284469240586 30.10651637145283 20.70742431036998 50-51 21.06924426244407 27.68538471637917 29.540807846922235 21.70456317425453 52-53 20.07309618019604 28.628702057787986 29.65212578844473 21.64607597357124 54-55 20.681149637480868 28.625468512071283 29.170218380398826 21.523163470049024 56-57 21.188734380213763 28.090624058878955 28.93256680619725 21.78807475471003 58-59 20.069217451706965 28.181442674803918 30.04776629154204 21.701573581947073 60-61 20.485291249934864 29.315197743483807 28.781602553708037 21.417908452873295 62-63 20.825309745233326 28.419859419961053 29.02973082012551 21.725100014680113 64-65 19.899564996300978 28.314789214050386 29.19239835532938 22.593247434319256 66-67 20.660067074758036 27.965210072533296 28.892036780303183 22.482686072405482 68-69 19.99833655412052 28.28224363937315 29.16623574519295 22.553184061313384 70-71 20.399618923951376 27.62564371065163 29.55840619657649 22.4163311688205 72-73 20.916198018032315 28.09241565433469 29.047750823328293 21.9436355043047 74-75 20.796187155714055 28.631519910263602 28.810423561488086 21.76186937253426 76-77 20.420766214650207 28.44087811415379 29.001311940224774 22.137043730971232 78-79 20.138326688908055 28.261038550670065 28.9545937695501 22.64604099087178 80-81 19.840230277450953 29.05591895121961 28.93185681848586 22.17199395284358 82-83 21.0765413267183 27.889521753560697 29.725959531357272 21.30797738836373 84-85 20.925746433244647 28.244903373765734 28.523693079562324 22.305657113427298 86-87 19.755563960321528 29.037219728971074 29.290211490023317 21.91700482068408 88-89 20.64820715013956 27.867630272860104 29.09498609276588 22.389176484234458 90-91 20.40010296770571 27.854029313762464 29.51957877206083 22.226288946471005 92-93 20.71502498456649 28.488426027544477 28.803136991050927 21.99341199683811 94-95 19.4164192973905 27.795985945017705 29.590735077873585 23.19685967971821 96-97 20.158163417820525 27.963836418590184 29.099825274808662 22.778174888780633 98-99 19.514774993484814 28.590877032981986 29.008649652089545 22.885698321443652 100 20.0333596187106 27.92576663333279 28.398821273756138 23.642052474200472 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5112.0 1 3289.0 2 1727.5 3 1927.0 4 1588.5 5 1590.5 6 2782.0 7 5522.5 8 8957.5 9 11010.0 10 10097.0 11 8878.5 12 9579.0 13 11123.5 14 13108.5 15 15467.5 16 18068.5 17 20362.5 18 22595.5 19 24597.0 20 26529.5 21 28426.5 22 30409.5 23 33089.0 24 35312.0 25 38561.0 26 46486.0 27 54861.5 28 60240.0 29 80650.0 30 122210.5 31 164489.0 32 231864.5 33 322310.0 34 403661.5 35 473271.0 36 560122.5 37 661417.0 38 668928.5 39 608206.5 40 528505.5 41 443759.0 42 395295.0 43 387182.5 44 405000.0 45 454061.0 46 474327.0 47 458756.0 48 455933.0 49 428335.5 50 390322.5 51 366331.0 52 346962.0 53 321089.0 54 302466.0 55 263277.5 56 224786.5 57 175186.0 58 125921.5 59 119235.0 60 95641.0 61 59710.0 62 40207.5 63 32506.0 64 21965.5 65 7159.0 66 2247.5 67 1327.0 68 1005.0 69 747.5 70 514.5 71 373.0 72 307.5 73 276.0 74 204.0 75 149.0 76 116.5 77 71.5 78 52.0 79 40.0 80 25.5 81 14.0 82 10.0 83 7.0 84 3.5 85 3.0 86 2.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030548653822654884 2 0.0021104849549522325 3 7.390803344190697E-5 4 0.024200774950366648 5 6.32324286114093E-4 6 4.927202229460465E-5 7 1.6424007431534884E-5 8 0.004303089947062139 9 0.01042924471902465 10-11 0.0010675604830497675 12-13 8.212003715767442E-5 14-15 1.1086205016286045E-4 16-17 3.5311615977799997E-4 18-19 2.8742013005186046E-5 20-21 9.033204087344185E-5 22-23 2.217241003257209E-4 24-25 3.284801486306977E-5 26-27 8.622603901555813E-5 28-29 0.0010634544811918838 30-31 0.002484131124019651 32-33 0.009530030312148116 34-35 0.01867409644965516 36-37 0.02160578177618414 38-39 0.02115822757367481 40-41 0.004463224019519605 42-43 0.005148926329786185 44-45 0.00925903418952779 46-47 0.012350853588514232 48-49 1.2051197572925878 50-51 0.01830866228430351 52-53 0.019528144836094975 54-55 0.010256792640993535 56-57 0.00815451968975707 58-59 0.008470681832814115 60-61 0.005333696413390953 62-63 0.007871205561563094 64-65 0.0201768931296406 66-67 0.030507593804076045 68-69 0.030967466012159023 70-71 0.027493788440389393 72-73 0.024061170887198603 74-75 0.009764072418047488 76-77 0.014925316753407325 78-79 0.008610285895982162 80-81 0.002734597237350558 82-83 0.014740546669802557 84-85 0.025178003392542975 86-87 0.024525149097139466 88-89 0.03395663536469837 90-91 0.021392269679574184 92-93 0.013792060240631418 94-95 0.006824175087802744 96-97 0.003777521709253023 98-99 0.015545323033947766 100 0.03691295670237465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2177296E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.19846425403011 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0860885987382 24.652335241567954 2 14.769550837759907 10.101919127464253 3 5.5265574727628115 5.66999334540366 4 2.6729198313162095 3.656390132206223 5 1.488559446929102 2.545322351790186 6 0.8889366481082706 1.824016091065682 7 0.5624719442902756 1.3464973676494063 8 0.39476793185339587 1.0800365604900604 9 0.284653084956052 0.8761228515602046 >10 1.0780288581580504 6.06486186944469 >50 0.0769930762957897 1.8735604140014799 >100 0.11306571935604155 8.944672891325322 >500 0.028998172042262165 6.997536653249396 >1k 0.026393250314689778 17.082431354069524 >5k 0.001621931583584279 3.58180988231708 >10k+ 3.931955354143707E-4 3.7024938663949256 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68318 0.5610276698538 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55215 0.45342578516609927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33011 0.271086454661199 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 16316 0.13398705262646157 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 13169 0.10814387693294143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12686 0.10417747913822575 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.212003715767442E-6 0.0 3 0.0 0.0 0.0 8.212003715767442E-6 0.0 4 0.0 0.0 0.0 8.212003715767442E-6 0.0 5 0.0 0.0 0.0 8.212003715767442E-6 0.0 6 0.0 0.0 0.0 8.212003715767442E-6 0.0 7 0.0 0.0 0.0 8.212003715767442E-6 0.0 8 0.0 0.0 0.0 8.212003715767442E-6 0.0 9 0.0 0.0 0.0 1.6424007431534884E-5 0.0 10-11 0.0 0.0 0.0 3.284801486306977E-5 0.0 12-13 0.0 0.0 0.0 3.284801486306977E-5 0.0 14-15 0.0 0.0 0.0 3.284801486306977E-5 0.0 16-17 0.0 0.0 0.0 6.980203158402326E-5 0.0 18-19 0.0 0.0 0.0 1.1086205016286046E-4 0.0 20-21 0.0 0.0 0.0 1.9298208732053488E-4 0.0 22-23 0.0 0.0 0.0 6.774903065508139E-4 0.0 24-25 0.0 0.0 0.0 0.002011940910363023 0.0 26-27 0.0 0.0 0.0 0.004323619956351558 0.0 28-29 0.0 0.0 0.0 0.009111218122643976 0.0 30-31 0.0 0.0 0.0 0.025026081323801276 0.0 32-33 0.0 0.0 0.0 0.05910179074237827 0.0 34-35 0.0 0.0 0.0 0.11217186475552537 0.0 36-37 0.0 0.0 0.0 0.1962586768031261 0.0 38-39 0.0 0.0 0.0 0.315932206953005 0.0 40-41 0.0 0.0 0.0 0.4409845995367116 0.0 42-43 0.0 0.0 0.0 0.5677943609155924 0.0 44-45 0.0 0.0 0.0 0.7176223687097694 0.0 46-47 0.0 0.0 0.0 0.9007213095583781 0.0 48-49 0.0 0.0 0.0 1.1041449596035113 0.0 50-51 0.0 0.0 0.0 1.3264603241967676 0.0 52-53 0.0 0.0 0.0 1.5396275166506588 0.0 54-55 0.0 0.0 0.0 1.7664348472764397 0.0 56-57 0.0 0.0 0.0 2.030237254641753 0.0 58-59 0.0 0.0 0.0 2.339792840709465 0.0 60-61 0.0 0.0 0.0 2.6582050727846314 0.0 62-63 0.0 0.0 0.0 2.9771305550920335 0.0 64-65 0.0 8.212003715767442E-6 0.0 3.3010160876437595 0.0 66-67 0.0 8.212003715767442E-6 0.0 3.6560004782670963 0.0 68-69 0.0 8.212003715767442E-6 0.0 4.039973242007092 0.0 70-71 0.0 8.212003715767442E-6 0.0 4.466824983148968 0.0 72-73 0.0 8.212003715767442E-6 0.0 4.896748013680542 0.0 74-75 0.0 8.212003715767442E-6 0.0 5.305865932798217 0.0 76-77 0.0 8.212003715767442E-6 0.0 5.75887701177667 0.0 78-79 0.0 8.212003715767442E-6 0.0 6.238642798861093 0.0 80-81 0.0 8.212003715767442E-6 0.0 6.762248367782142 0.0 82-83 0.0 8.212003715767442E-6 0.0 7.289927090546209 0.0 84-85 0.0 8.212003715767442E-6 0.0 7.78838339808772 0.0 86-87 0.0 8.212003715767442E-6 0.0 8.30744772895395 0.0 88 0.0 8.212003715767442E-6 0.0 8.7120736820391 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 31190 0.0 54.56031 1 GTATCAA 59930 0.0 42.62442 1 TACCCCG 580 0.0 38.820244 5 ACCTGGG 2830 0.0 37.63542 3 TACCTGG 2990 0.0 35.77694 2 CAACGCA 74095 0.0 33.673332 5 ATCAACG 74540 0.0 33.51244 3 TATTCTC 7855 0.0 33.142998 5 GTACTAG 9970 0.0 32.66847 1 TAGGCAT 11540 0.0 32.274513 5 TATCAAC 79670 0.0 31.889166 2 TCTCTGC 8260 0.0 31.687927 8 AACGCAG 81440 0.0 31.240759 6 CATGGGT 4310 0.0 30.590488 4 TATCCGC 340 0.0 30.367441 1 TCAAGAC 1745 0.0 29.845919 3 CCTCAGG 6820 0.0 29.788118 2 TCAACGC 85200 0.0 29.545238 4 ATGGGTA 3105 0.0 29.005785 5 CCCGGCG 795 0.0 28.911366 8 >>END_MODULE