FastQCFastQC Report
Mon 6 Jun 2016
ERR1211305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8594207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332730.3871561390131748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266230.3097784356369354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151520.17630480624913966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159550.054.3909531
GTATCAA288300.042.092261
CAACGCA356650.033.4274445
ATCAACG360150.033.115643
TATCAAC370500.032.6124042
AACGCAG381500.031.5589226
TCAACGC381300.031.339854
GTACTAG35050.027.9127371
ACGCAGA426750.027.6845137
GTGGTAT65350.027.494441
CGCAGAG438650.026.856918
GTACATG403250.026.7692051
TACATGG414250.025.7165412
ACATGGG417150.024.5803173
GCAGAGT475300.024.2827649
TGGTATC75200.023.0789172
GAGTACT248150.022.5638712-13
CATGGGG276650.022.013634
CAGAGTA464850.021.21516410-11
CTAGTAC47450.020.8963833
Produced by FastQC (version 0.11.5)