FastQCFastQC Report
Mon 6 Jun 2016
ERR1211305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8594207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293770.3418232770050803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248150.2887410089144932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146200.17011458997904055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157900.053.8280561
GTATCAA286550.042.125291
CAACGCA361900.032.498725
ATCAACG363700.032.3586853
TATCAAC374850.031.9449332
AACGCAG387500.030.6664836
TCAACGC385750.030.618684
GTACTAG36400.029.655061
GTGGTAT69350.027.5435731
ACGCAGA440150.026.774387
CGCAGAG453700.025.9127068
GTACATG393250.024.1434611
GCAGAGT491950.023.6296929
TACATGG403400.023.2982832
TGGTATC79000.022.9859622
ACATGGG407350.022.3711413
GAGTACT260950.021.85828612-13
TAGGCAT52350.021.6869855
CTAGGCA55600.021.5215994
TAGTACT48250.021.4933514
Produced by FastQC (version 0.11.5)