##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211305_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8594207 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07917833489466 33.0 31.0 34.0 30.0 34.0 2 32.215287227780294 33.0 31.0 34.0 30.0 34.0 3 32.27532127164263 34.0 31.0 34.0 30.0 34.0 4 35.92480062442061 37.0 35.0 37.0 35.0 37.0 5 35.826880944338434 37.0 35.0 37.0 35.0 37.0 6 35.84496114650252 37.0 35.0 37.0 35.0 37.0 7 35.75780860293451 37.0 35.0 37.0 33.0 37.0 8 35.74129527017443 37.0 35.0 37.0 33.0 37.0 9 37.34937801707592 39.0 37.0 39.0 34.0 39.0 10-11 37.30088529401259 39.0 37.0 39.0 34.0 39.0 12-13 37.228973249073476 39.0 37.0 39.0 33.0 39.0 14-15 38.44366525032501 40.0 38.0 41.0 34.0 41.0 16-17 38.42555264261147 40.0 38.0 41.0 34.0 41.0 18-19 38.43976791576 40.0 38.0 41.0 34.0 41.0 20-21 38.41386826032931 40.0 38.0 41.0 34.0 41.0 22-23 38.30328470096194 40.0 38.0 41.0 34.0 41.0 24-25 38.20439913769822 40.0 38.0 41.0 33.0 41.0 26-27 38.09912653953995 40.0 37.5 41.0 33.0 41.0 28-29 37.99942478695242 40.0 37.0 41.0 33.0 41.0 30-31 38.00439255186662 40.0 37.0 41.0 33.0 41.0 32-33 37.93889087149053 40.0 37.0 41.0 33.0 41.0 34-35 37.806869906670855 40.0 37.0 41.0 33.0 41.0 36-37 37.65640157375776 40.0 37.0 41.0 33.0 41.0 38-39 37.53942056550418 40.0 36.0 41.0 32.0 41.0 40-41 37.42286606547876 40.0 36.0 41.0 32.0 41.0 42-43 37.30913945870748 40.0 36.0 41.0 31.5 41.0 44-45 37.1315917803702 39.0 36.0 41.0 31.0 41.0 46-47 36.866594265183515 39.0 35.5 41.0 30.5 41.0 48-49 35.29831664515412 39.0 35.0 40.0 24.0 41.0 50-51 36.00435363030004 38.5 35.0 40.0 28.0 41.0 52-53 36.08705474513239 38.5 35.0 40.0 29.0 41.0 54-55 35.90796271255742 38.0 35.0 40.0 28.5 41.0 56-57 35.676216490945585 38.0 34.0 40.0 28.0 41.0 58-59 35.424751405219816 38.0 34.0 40.0 28.0 41.0 60-61 35.161611594880135 37.0 34.0 40.0 27.5 41.0 62-63 34.833764883717606 37.0 34.0 39.5 27.0 41.0 64-65 34.48922402031974 36.0 33.0 39.0 26.0 41.0 66-67 34.14725890358471 36.0 33.0 39.0 26.0 40.0 68-69 33.789998076611376 35.0 33.0 38.5 26.0 40.0 70-71 33.43923232242369 35.0 32.0 38.0 26.0 40.0 72-73 33.06868586013812 35.0 32.0 37.0 25.0 39.5 74-75 32.71618358738625 35.0 32.0 36.5 25.0 39.0 76-77 31.80331105592407 34.0 30.5 35.5 23.5 38.0 78-79 32.251076975455675 34.5 31.0 36.0 25.0 37.5 80-81 32.13515889249584 34.5 31.0 35.0 25.0 37.0 82-83 31.881096243085604 34.0 31.0 35.0 24.0 37.0 84-85 31.61178186655267 34.0 31.0 35.0 24.0 36.0 86-87 31.361955326419295 34.0 31.0 35.0 23.0 36.0 88-89 31.142482837567215 34.0 31.0 35.0 23.0 35.5 90-91 30.927845524316552 34.0 30.0 35.0 21.5 35.0 92-93 30.73618298930896 34.0 30.0 35.0 20.0 35.0 94-95 30.546058117985755 34.0 30.0 35.0 20.0 35.0 96-97 30.356099463277996 34.0 29.0 35.0 19.5 35.0 98-99 30.139687873471047 34.0 29.0 35.0 19.0 35.0 100 27.65868148160732 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3008877930688598 1101 2 -0.27249152613824634 1101 3 -0.2952596482570442 1101 4 -0.17607565405480585 1101 5 -0.1659770637972997 1101 6 -0.20293043501267505 1101 7 -0.23487720260658307 1101 8 -0.23188751071872815 1101 9 -0.35973620779667925 1101 10-11 -0.35221712270361394 1101 12-13 -0.41421480253669074 1101 14-15 -0.5502921713218427 1101 16-17 -0.5257310590346904 1101 18-19 -0.5593819080141671 1101 20-21 -0.4982559039759096 1101 22-23 -0.6750233625286128 1101 24-25 -0.6264497424327402 1101 26-27 -0.6232813986399321 1101 28-29 -0.6922667856501192 1101 30-31 -0.6125909109249008 1101 32-33 -0.5355627495813806 1101 34-35 -0.628725731470233 1101 36-37 -0.6795071548735905 1101 38-39 -0.6180629292278681 1101 40-41 -0.7240118634199675 1101 42-43 -0.7028380523528739 1101 44-45 -0.6425399292018099 1101 46-47 -0.6717811341207707 1101 48-49 0.5824428711811578 1101 50-51 -0.3102795589264957 1101 52-53 -0.6859443929673077 1101 54-55 -0.6872944199897475 1101 56-57 -0.8043483035586689 1101 58-59 -0.7923928508503266 1101 60-61 -0.7474531085269049 1101 62-63 -0.8019716944923445 1101 64-65 -0.7785941185740697 1101 66-67 -0.7390484592019035 1101 68-69 -0.8708185584582964 1101 70-71 -0.8022543487291856 1101 72-73 -0.8304804264945176 1101 74-75 -0.8705896876084793 1101 76-77 -0.7425903514697048 1101 78-79 -0.6733157502011693 1101 80-81 -0.6311127649911796 1101 82-83 -0.6901434523296537 1101 84-85 -0.7829137605778129 1101 86-87 -0.7337149924680695 1101 88-89 -0.7523450077841467 1101 90-91 -0.789756329327318 1101 92-93 -0.767439550168266 1101 94-95 -0.8376732911585982 1101 96-97 -0.9682352218926802 1101 98-99 -1.105107797215414 1101 100 -0.9471769645729182 1102 1 -0.2532514542966595 1102 2 -0.2323131592881431 1102 3 -0.2647420819011188 1102 4 -0.17102406376815082 1102 5 -0.14561661032772122 1102 6 -0.2292689789666511 1102 7 -0.1793946371637105 1102 8 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-0.6653492470331557 1102 72-73 -0.7284576124987936 1102 74-75 -0.7928315247003717 1102 76-77 -0.6883908217601196 1102 78-79 -0.5473849252045397 1102 80-81 -0.5358878673262595 1102 82-83 -0.6365508662228088 1102 84-85 -0.5699442327850512 1102 86-87 -0.7337502825052908 1102 88-89 -0.7273116563666839 1102 90-91 -0.7285332216747378 1102 92-93 -0.8137040249343706 1102 94-95 -0.8801444649163308 1102 96-97 -0.8561755208005835 1102 98-99 -0.9441127735449548 1102 100 -0.7581268840693305 1103 1 -0.31266131843441514 1103 2 -0.30267844119827103 1103 3 -0.31090525084766085 1103 4 -0.17200906444222142 1103 5 -0.2524246716142997 1103 6 -0.26296211316228835 1103 7 -0.2644026975360205 1103 8 -0.2807197578430731 1103 9 -0.38670394934419505 1103 10-11 -0.3802194302628408 1103 12-13 -0.4841713988722063 1103 14-15 -0.536222252342391 1103 16-17 -0.5661723265084859 1103 18-19 -0.619434430259453 1103 20-21 -0.6505607205547719 1103 22-23 -0.6952071107488464 1103 24-25 -0.7559096089346369 1103 26-27 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-0.8480491710748019 1103 92-93 -0.8366451333303999 1103 94-95 -0.9868752467167106 1103 96-97 -1.0602011516697765 1103 98-99 -1.1609798715149857 1103 100 -1.0692467011970024 1104 1 -0.3515031404979503 1104 2 -0.32292365228216724 1104 3 -0.3263901662973403 1104 4 -0.17029705973244091 1104 5 -0.17534252745215184 1104 6 -0.31392593147281644 1104 7 -0.259077562495861 1104 8 -0.24934858945209726 1104 9 -0.4451201794445723 1104 10-11 -0.39947536074310364 1104 12-13 -0.443129870944972 1104 14-15 -0.6188344192281363 1104 16-17 -0.6589420960283192 1104 18-19 -0.6246956624576399 1104 20-21 -0.6228509471406554 1104 22-23 -0.6331313500402374 1104 24-25 -0.6799946238226653 1104 26-27 -0.7117613071479099 1104 28-29 -0.7124339886113589 1104 30-31 -0.6657686647599164 1104 32-33 -0.6416640957404596 1104 34-35 -0.680278186146289 1104 36-37 -0.6866494326000847 1104 38-39 -0.6144998570652902 1104 40-41 -0.7634375285154107 1104 42-43 -0.7767660760758375 1104 44-45 -0.764234755745413 1104 46-47 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1105 68-69 -0.6605141493183808 1105 70-71 -0.7280157970243266 1105 72-73 -0.6661932474836263 1105 74-75 -0.7584350762423036 1105 76-77 -0.7241573345609673 1105 78-79 -0.5851794056063753 1105 80-81 -0.5812956798087328 1105 82-83 -0.5318432158197091 1105 84-85 -0.5171073742629986 1105 86-87 -0.4345948841242482 1105 88-89 -0.5824242728079732 1105 90-91 -0.6244704291015744 1105 92-93 -0.5915315037625106 1105 94-95 -0.5888439017194749 1105 96-97 -0.6813038013617003 1105 98-99 -0.7648731906292987 1105 100 -0.6089255927392614 1106 1 -0.34120658369060664 1106 2 -0.35205445343103037 1106 3 -0.35987841829959777 1106 4 -0.23839164911560573 1106 5 -0.2519269411797893 1106 6 -0.3147091487442495 1106 7 -0.36466211181475217 1106 8 -0.32125503367308283 1106 9 -0.4742351423440283 1106 10-11 -0.4088212979019019 1106 12-13 -0.45921457935116194 1106 14-15 -0.600025571519538 1106 16-17 -0.5604930344279708 1106 18-19 -0.6064578628314266 1106 20-21 -0.6310206883837282 1106 22-23 -0.5981422892815331 1106 24-25 -0.5109754702950582 1106 26-27 -0.6131469496576187 1106 28-29 -0.6452964175294866 1106 30-31 -0.7068480709161591 1106 32-33 -0.7242384166070153 1106 34-35 -0.6990714806939664 1106 36-37 -0.6611097961217993 1106 38-39 -0.7092118282841469 1106 40-41 -0.6684612248330666 1106 42-43 -0.6643028720908006 1106 44-45 -0.6664937668421871 1106 46-47 -0.6690400602327102 1106 48-49 0.5636948300606264 1106 50-51 -0.31639289652750335 1106 52-53 -0.6285795130677911 1106 54-55 -0.817703335764044 1106 56-57 -0.8364811341695955 1106 58-59 -0.80303142525743 1106 60-61 -0.778414971861352 1106 62-63 -0.7579253211034853 1106 64-65 -0.8011191363748509 1106 66-67 -0.7606863565568389 1106 68-69 -0.7882242238920796 1106 70-71 -0.781320314636524 1106 72-73 -0.7998752515980243 1106 74-75 -0.8150253071352402 1106 76-77 -0.7393199987390275 1106 78-79 -0.6773269395561456 1106 80-81 -0.5768084578371528 1106 82-83 -0.585839531966478 1106 84-85 -0.6208095443220358 1106 86-87 -0.6083647485423818 1106 88-89 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0.48200786463518597 2311 10-11 0.3895369732415972 2311 12-13 0.48249794946592317 2311 14-15 0.46633052883871073 2311 16-17 0.5003962890923574 2311 18-19 0.6146752992894093 2311 20-21 0.6519216700206059 2311 22-23 0.685311769133989 2311 24-25 0.6512266654989887 2311 26-27 0.730642482162196 2311 28-29 0.7773967383479885 2311 30-31 0.6144872188000576 2311 32-33 0.6371305200485367 2311 34-35 0.7140832064340472 2311 36-37 0.801560466966599 2311 38-39 0.7979418589182714 2311 40-41 0.7754591571743958 2311 42-43 0.7914541136594906 2311 44-45 0.8498305714921699 2311 46-47 0.9369312273308239 2311 48-49 -5.998429782108541 2311 50-51 -1.025305781413394 2311 52-53 0.4005758823924168 2311 54-55 0.8265308069304709 2311 56-57 0.9287100698883961 2311 58-59 0.9858031308345403 2311 60-61 0.8969952621543484 2311 62-63 0.7416959361946667 2311 64-65 0.822568790003146 2311 66-67 0.9608543595501473 2311 68-69 0.9548641423665529 2311 70-71 0.9759307121667078 2311 72-73 0.5199084441077915 2311 74-75 0.8292296608969991 2311 76-77 0.8690595448005034 2311 78-79 0.7939414615730414 2311 80-81 0.8246554029120716 2311 82-83 0.8502920577156594 2311 84-85 0.8724287759700324 2311 86-87 0.9362734830496002 2311 88-89 0.9570299523697336 2311 90-91 1.066130873208099 2311 92-93 1.044193506442113 2311 94-95 1.0113972336044021 2311 96-97 1.0493934698652758 2311 98-99 1.1617022682652411 2311 100 1.3560311657518014 2312 1 0.10657810810232604 2312 2 0.26163253052376234 2312 3 0.27799120764172613 2312 4 0.06655474333324207 2312 5 0.2209153834193529 2312 6 0.1656561839320574 2312 7 0.2971726330980502 2312 8 0.37211898443909064 2312 9 0.3368919076155379 2312 10-11 0.27876760197968053 2312 12-13 0.3459242994450733 2312 14-15 0.3985140359070627 2312 16-17 0.5032027298099138 2312 18-19 0.40027692968077844 2312 20-21 0.4218255451410897 2312 22-23 0.6685378832261577 2312 24-25 0.7379463114584652 2312 26-27 0.6678765513846798 2312 28-29 0.7696320296906904 2312 30-31 0.8110190037926159 2312 32-33 0.7850629962546591 2312 34-35 0.4280199567579288 2312 36-37 0.7378178033932627 2312 38-39 0.8384896673213262 2312 40-41 0.6102852578511886 2312 42-43 0.6484253419174166 2312 44-45 0.7625873321333572 2312 46-47 0.8502382384981857 2312 48-49 -4.656772533305276 2312 50-51 -0.7538335927790101 2312 52-53 0.5400121140139262 2312 54-55 0.8267079953069683 2312 56-57 0.8974307581648233 2312 58-59 0.9090352636359 2312 60-61 0.9360186386125164 2312 62-63 0.726146944206711 2312 64-65 0.9443813789255273 2312 66-67 0.9430255691925495 2312 68-69 0.8770295772156445 2312 70-71 0.8788725897211052 2312 72-73 0.9956498764140562 2312 74-75 0.7073750007188266 2312 76-77 0.9149700620554846 2312 78-79 0.5768613511265741 2312 80-81 0.8023264279660438 2312 82-83 0.848124350552947 2312 84-85 0.8800095631560474 2312 86-87 0.9561570318462067 2312 88-89 1.0278002306938525 2312 90-91 0.8967404050344214 2312 92-93 1.0402039889814176 2312 94-95 1.1602329290683038 2312 96-97 1.1633543068829688 2312 98-99 1.1797434475341895 2312 100 0.7868225101933 2313 1 0.14023898654280487 2313 2 0.2601870296747464 2313 3 0.3397156824712937 2313 4 0.11270705617924648 2313 5 0.1384302582585164 2313 6 0.28471049744800325 2313 7 0.17229175297928379 2313 8 0.2863183130879463 2313 9 0.452618630831239 2313 10-11 0.2662932110279925 2313 12-13 -0.002127842995463425 2313 14-15 0.13763741554079445 2313 16-17 0.12061734158702819 2313 18-19 0.47435855228155077 2313 20-21 0.4445878175232494 2313 22-23 0.5930288882383934 2313 24-25 0.3714149274878551 2313 26-27 0.526076437883475 2313 28-29 0.5161953882812895 2313 30-31 0.6799618989088216 2313 32-33 0.5835737898225872 2313 34-35 0.5418636975970088 2313 36-37 -0.203404370377946 2313 38-39 0.2933220850565803 2313 40-41 0.5205055240638075 2313 42-43 0.6663807269625224 2313 44-45 0.6450810397608038 2313 46-47 0.5255131177928476 2313 48-49 -5.300290354922829 2313 50-51 -1.198772750192596 2313 52-53 0.24786617050856563 2313 54-55 0.3981826028242068 2313 56-57 0.40783598141288735 2313 58-59 0.43351901830161665 2313 60-61 0.7828419340143071 2313 62-63 0.9062531676495809 2313 64-65 1.0371837066948046 2313 66-67 0.9725941082663141 2313 68-69 1.0370636061869973 2313 70-71 1.0220582482844165 2313 72-73 0.7171256655000136 2313 74-75 0.8635740137704744 2313 76-77 0.8742842799881281 2313 78-79 0.89461896461858 2313 80-81 -0.0415122383572637 2313 82-83 0.38856016542492355 2313 84-85 0.7854199154298662 2313 86-87 0.8870442742868043 2313 88-89 0.9017458129963494 2313 90-91 0.9109817707417953 2313 92-93 1.0299567719962504 2313 94-95 0.9147474196795962 2313 96-97 0.9341858253800517 2313 98-99 1.0706315311935732 2313 100 1.166146706463369 2314 1 0.08365259898085498 2314 2 0.20272427560276896 2314 3 0.22238953982517273 2314 4 0.06916062203006135 2314 5 0.2007500348725415 2314 6 0.252136969860139 2314 7 0.07389307308332604 2314 8 0.21839490439494824 2314 9 0.396430508637593 2314 10-11 0.3168977579608807 2314 12-13 0.22060487499642534 2314 14-15 0.1913037190933835 2314 16-17 0.4993504858917106 2314 18-19 0.5526588045969234 2314 20-21 0.5906398511937638 2314 22-23 0.3352610145491255 2314 24-25 0.48787078692317465 2314 26-27 0.567855352379361 2314 28-29 0.6367905965467955 2314 30-31 0.551915808541338 2314 32-33 0.5545598053917971 2314 34-35 0.46118558299775003 2314 36-37 0.5463775719546433 2314 38-39 0.5495279512101163 2314 40-41 0.3933268007381088 2314 42-43 0.3182489919735616 2314 44-45 0.6329718763014753 2314 46-47 0.745026731518827 2314 48-49 -6.019183281398018 2314 50-51 -1.4066898539374506 2314 52-53 -0.7852321420213642 2314 54-55 -0.3565378240523458 2314 56-57 -0.006459005817049501 2314 58-59 0.46045367885959365 2314 60-61 0.7013904523961685 2314 62-63 0.6162888104626205 2314 64-65 0.7532848154242657 2314 66-67 0.8401987450533497 2314 68-69 0.8574859282933431 2314 70-71 0.6197971863493663 2314 72-73 0.7528050435884737 2314 74-75 0.8715662743683339 2314 76-77 0.7757446402115953 2314 78-79 0.5731189097634868 2314 80-81 0.5861531940789249 2314 82-83 0.6105605033793005 2314 84-85 0.6240157024520236 2314 86-87 0.7135695458727405 2314 88-89 0.693897818943686 2314 90-91 0.8135003339143054 2314 92-93 0.9032115389513322 2314 94-95 0.9601203945344814 2314 96-97 0.9409571698324442 2314 98-99 1.0009079829119436 2314 100 -0.4134143899226217 2315 1 0.0986507224160178 2315 2 0.1945486375462906 2315 3 0.30987354795124133 2315 4 0.13698153033996618 2315 5 0.22471088431854724 2315 6 0.28350319347622843 2315 7 0.34683756962412815 2315 8 0.25729336394714863 2315 9 0.42555600138457805 2315 10-11 0.2177982316423197 2315 12-13 0.38505150518744813 2315 14-15 0.2323799821689292 2315 16-17 0.3270661010092013 2315 18-19 0.48273173327844887 2315 20-21 0.37844879684097776 2315 22-23 0.4149000456798788 2315 24-25 0.46183831613630133 2315 26-27 0.5316846923118419 2315 28-29 0.6096018117153648 2315 30-31 0.6550649640437172 2315 32-33 0.6956529655975672 2315 34-35 0.6656781452639109 2315 36-37 0.5567279626911485 2315 38-39 0.6494238266190493 2315 40-41 0.7078493401562156 2315 42-43 0.7524539127350209 2315 44-45 0.7147176480276514 2315 46-47 0.5971374969565559 2315 48-49 -4.928346663051869 2315 50-51 -0.9689231081670613 2315 52-53 0.2158472789238246 2315 54-55 0.1353383232412284 2315 56-57 0.4410602641337178 2315 58-59 0.5991363272850165 2315 60-61 0.49901559500211334 2315 62-63 0.3050204644163088 2315 64-65 0.49679991474387464 2315 66-67 -0.2429910036471128 2315 68-69 0.5887420036306494 2315 70-71 0.4942235623964564 2315 72-73 0.5836410543330075 2315 74-75 0.7515117938104936 2315 76-77 0.738887802041031 2315 78-79 0.6457686612713118 2315 80-81 0.5555917463971056 2315 82-83 0.5452328399938331 2315 84-85 0.5542976583301034 2315 86-87 0.6322548958248575 2315 88-89 0.5283147989857717 2315 90-91 0.6527983275622518 2315 92-93 0.6661116867948031 2315 94-95 0.2565159238701611 2315 96-97 0.4238470384073487 2315 98-99 0.40677136905123135 2315 100 0.4566239601651212 2316 1 0.20651896754527854 2316 2 0.23172709084308707 2316 3 0.17022408387371257 2316 4 -0.03158108467910381 2316 5 0.18396769741267605 2316 6 0.15265347591492429 2316 7 0.27220359698655017 2316 8 0.3032919593909753 2316 9 0.4407940931964873 2316 10-11 0.4039729653264956 2316 12-13 0.3332726503630994 2316 14-15 0.4857133359470396 2316 16-17 0.41666410715593116 2316 18-19 0.09279220401392507 2316 20-21 0.44317198203184915 2316 22-23 0.6039421298377405 2316 24-25 0.6128730243297724 2316 26-27 0.6275109245254242 2316 28-29 0.6695725397094421 2316 30-31 0.6230338814169016 2316 32-33 0.5691016542597467 2316 34-35 0.5564020560870944 2316 36-37 0.4427116242083642 2316 38-39 0.5613748907480627 2316 40-41 0.11168718453128434 2316 42-43 0.1018640357068108 2316 44-45 0.36573762222083417 2316 46-47 0.3632983460816135 2316 48-49 -5.208195024927882 2316 50-51 -1.1279397718004276 2316 52-53 0.23068924335392893 2316 54-55 0.5825175711321435 2316 56-57 0.4316627437942131 2316 58-59 0.33528036628202074 2316 60-61 0.4100096568166549 2316 62-63 0.29999590001685306 2316 64-65 0.34780994963013967 2316 66-67 0.45134300951436757 2316 68-69 0.11477750799091524 2316 70-71 -0.22961485875722332 2316 72-73 -0.10701506141336381 2316 74-75 0.08718937146142025 2316 76-77 0.12775636410364655 2316 78-79 0.20776458694181343 2316 80-81 0.12021199999961851 2316 82-83 0.022110313344949617 2316 84-85 0.14003148860519232 2316 86-87 0.24918585349870526 2316 88-89 0.10705625016940346 2316 90-91 0.22960601738541797 2316 92-93 0.28591776985508943 2316 94-95 0.5064810694572195 2316 96-97 0.5381396664257565 2316 98-99 0.5719641245703464 2316 100 -0.18467230120467804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 6.0 11 17.0 12 45.0 13 175.0 14 689.0 15 2034.0 16 4783.0 17 9816.0 18 17368.0 19 26277.0 20 38517.0 21 54957.0 22 69455.0 23 73209.0 24 76021.0 25 82175.0 26 96655.0 27 117758.0 28 144505.0 29 177494.0 30 218496.0 31 270031.0 32 340670.0 33 443016.0 34 612905.0 35 868198.0 36 1344563.0 37 1911345.0 38 1419934.0 39 173079.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97399557316482 20.15746376353619 14.504944370089659 23.363596293209334 2 15.422777837961682 20.205563653555277 40.15063249146588 24.221026017017163 3 18.64400113751187 25.597728978952773 29.87001925485525 25.888250628680098 4 12.852270959066631 16.818356443412323 37.78801961308487 32.54135298443618 5 13.560752645194665 37.79376458619792 34.26660564855078 14.378877120056636 6 31.370440895929292 37.02826497622526 17.275408254748402 14.325885873097047 7 27.991524521465337 32.376560717367106 21.57383958143889 18.058075179728668 8 26.589718733007494 35.21696820161085 19.393176270089484 18.800136795292175 9 26.499296255286815 15.845933728718196 20.374709876223555 37.280060139771436 10-11 25.464079360380687 26.225432016710975 27.14067491789728 21.16981370501106 12-13 25.875315976310294 23.713996997857798 27.80411758097456 22.606569444857353 14-15 23.341657552961014 25.064598806706712 25.42904231098209 26.16470132935018 16-17 22.406956477624327 28.128603820935965 26.329059780826018 23.13537992061369 18-19 22.192183896918458 27.124396366575343 28.687589057092648 21.99583067941355 20-21 23.249579760211553 26.18529204665402 28.55757786893615 22.007550324198277 22-23 23.292867800809304 25.90418534668755 28.357401832040765 22.44554502046238 24-25 22.88035949805712 26.173700766970303 28.58838601127155 22.35755372370103 26-27 22.674823488453896 26.40779004716787 28.299613099397185 22.617773364981044 28-29 22.424703839436145 26.361439140872413 28.65660315051692 22.557253869174524 30-31 22.8543728114765 26.174435613584812 28.52363265025432 22.447558924684362 32-33 22.854895620312796 26.496924591231853 28.13484820723972 22.51333158121563 34-35 22.927860837213977 26.406918849871786 28.437627216402127 22.22759309651211 36-37 22.9280716655428 26.23263020465395 28.20161822866319 22.63767990114006 38-39 22.88453020194372 26.265526231688163 28.434508473424287 22.415435092943827 40-41 22.706551592647372 25.975866479135533 28.73761036043191 22.579971567785186 42-43 22.661396152831326 26.073089979342477 28.361463847593406 22.90405002023279 44-45 22.657084214240296 26.46008874701283 28.166243784776622 22.71658325397025 46-47 22.739891354495047 26.379657161702657 27.809658046707174 23.07079343709512 48-49 23.137135791552776 26.42285978496655 27.670814837880492 22.76918958560018 50-51 22.940767331516003 26.476615446331824 27.484762119618882 23.097855102533288 52-53 22.71729982653104 26.72314043118596 27.418709591336317 23.140850150946683 54-55 22.708599817161836 26.67072559939079 27.377611774256312 23.243062809191063 56-57 23.02227569897566 26.524585423440755 27.175718554277434 23.27742032330615 58-59 22.618426996521332 26.582668898735584 27.57123330806624 23.227670796676847 60-61 22.703746762343474 26.844258408365203 27.327345445718787 23.12464938357254 62-63 22.776148133287748 26.65924288868476 27.260030129545875 23.30457884848162 64-65 22.470605028161785 26.519684097338132 27.374898364385984 23.634812510114102 66-67 22.67916675468685 26.434657686454827 27.274576820785494 23.61159873807283 68-69 22.42769979466339 26.587768074149302 27.30313489248867 23.68139723869863 70-71 22.536774384874462 26.276922471707937 27.393864468587353 23.792438674830244 72-73 22.709057024381426 26.522446948332544 27.147621742742896 23.620874284543127 74-75 22.694607663679342 26.7251401014125 27.063765547073356 23.516486687834806 76-77 22.595761728358784 26.592232114309223 26.996787795192777 23.815218362139216 78-79 22.49744182288949 26.47953461220758 27.07739196323649 23.94563160166644 80-81 22.39546431286369 26.85614544787694 26.99112948728406 23.757260751975306 82-83 22.771085206644877 26.406230307434154 27.310905691323907 23.51177879459706 84-85 22.79433518377262 26.502840834366637 26.870616515409218 23.832207466451525 86-87 22.318595718440033 26.751099579034193 27.175178653406366 23.75512604911941 88-89 22.48113053214422 26.3082572068526 27.129933759209802 24.08067850179338 90-91 22.562653022925616 26.263285990482938 27.171855565270143 24.002205421321303 92-93 22.605432515178038 26.385410885011524 27.009208501969283 23.999948097841155 94-95 22.180435619802893 26.156987645606556 27.29726385027759 24.36531288431296 96-97 22.342348716020098 26.124000221552397 27.23456068394527 24.299090378482234 98-99 22.10237209568635 26.286403857692882 27.135452864152626 24.475771182468147 100 22.188747564089102 26.03402395136815 27.001229857103315 24.77599862743943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2093.0 1 1476.0 2 1152.0 3 1437.0 4 1209.5 5 1148.5 6 1818.5 7 3605.0 8 5954.0 9 7345.0 10 6686.5 11 5500.5 12 5603.0 13 6347.0 14 7239.0 15 8233.5 16 9129.0 17 9862.0 18 10787.5 19 11718.5 20 12598.0 21 13673.5 22 15181.5 23 17382.5 24 19943.5 25 22980.5 26 27661.0 27 33664.0 28 40242.0 29 50843.5 30 66662.0 31 82338.0 32 103614.5 33 130032.0 34 154223.0 35 177795.0 36 207387.0 37 241291.0 38 256278.5 39 255795.5 40 249470.5 41 242807.0 42 246325.5 43 258957.0 44 277012.5 45 303118.0 46 325728.5 47 339010.0 48 352326.0 49 357796.0 50 354002.0 51 350100.5 52 345282.0 53 338166.0 54 328803.5 55 306632.5 56 277205.5 57 241773.5 58 208918.0 59 184923.0 60 154850.5 61 124478.5 62 99772.5 63 77552.5 64 57095.5 65 40332.0 66 29421.0 67 22150.0 68 16669.0 69 12302.0 70 9342.5 71 7034.5 72 5242.0 73 3991.0 74 2985.5 75 2176.0 76 1402.0 77 806.0 78 510.0 79 335.0 80 202.5 81 116.0 82 64.0 83 37.0 84 29.0 85 23.5 86 15.5 87 8.5 88 5.5 89 5.5 90 2.5 91 1.0 92 2.0 93 1.0 94 0.0 95 0.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02927553408941628 2 0.0020711625866121214 3 1.2799319355468166E-4 4 0.020711625866121214 5 4.421583050070821E-4 6 2.3271489737214847E-5 7 2.3271489737214847E-5 8 0.004002696234800954 9 0.010379084422797823 10-11 0.0011053957625177053 12-13 1.2217532112037796E-4 14-15 9.308595894885939E-5 16-17 3.490723460582227E-4 18-19 1.1635744868607423E-5 20-21 7.563234164594825E-5 22-23 2.3853276980645216E-4 24-25 2.3271489737214847E-5 26-27 1.1635744868607423E-4 28-29 0.0010879421452147941 30-31 0.0026529498300424926 32-33 0.009267870787845814 34-35 0.01820994071937062 36-37 0.021514492262055127 38-39 0.021095605446785258 40-41 0.005049913272975622 42-43 0.005637518388840297 44-45 0.009203874191068473 46-47 0.01199063508709995 48-49 1.2002968976660673 50-51 0.01813430837772467 52-53 0.018657916896812003 54-55 0.00948313206791505 56-57 0.007557416292160522 58-59 0.007970485234996086 60-61 0.004817198375603474 62-63 0.007551598419726218 64-65 0.018890631794184154 66-67 0.028245770668544518 68-69 0.028635568121642873 70-71 0.02584298935317709 72-73 0.02292241739115662 74-75 0.009180602701331258 76-77 0.013957075969894605 78-79 0.007976303107430388 80-81 0.0026529498300424926 82-83 0.013730178944956758 84-85 0.023422754420506744 86-87 0.022590798662401313 88-89 0.031980844771367506 90-91 0.020298556923285648 92-93 0.012979673400931582 94-95 0.006324027336088134 96-97 0.0035256306951880496 98-99 0.015114832584321045 100 0.03449998353542101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8594207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.087578128335274 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01630930610987 36.990675593680216 2 15.462712030331167 16.108304419116486 3 5.797270407313397 9.05897325816068 4 2.7812362846510914 5.794714490804987 5 1.4795537969599415 3.853318699711303 6 0.882739913418191 2.7587870524301965 7 0.532771089558348 1.9425529026322135 8 0.39316307287628594 1.6383129820495934 9 0.2895244630502004 1.3572565280272468 >10 1.2576081251298579 11.146793248456307 >50 0.06413844492540767 2.3011175126172354 >100 0.03883211189992565 3.846834539625985 >500 0.002848986107220733 1.0102865872319282 >1k 0.0011594581647192855 0.9022769139551141 >5k 3.312737612251953E-5 0.10242715165124924 >10k+ 9.93821283675586E-5 1.1873681198493535 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29377 0.3418232770050803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24815 0.2887410089144932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14620 0.17011458997904055 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1635744868607423E-5 7 0.0 0.0 0.0 0.0 1.1635744868607423E-5 8 0.0 1.1635744868607423E-5 0.0 0.0 4.6542979474429694E-5 9 0.0 1.1635744868607423E-5 0.0 0.0 5.817872434303712E-5 10-11 0.0 1.1635744868607423E-5 0.0 0.0 5.817872434303712E-5 12-13 0.0 1.1635744868607423E-5 0.0 0.0 5.817872434303712E-5 14-15 0.0 1.1635744868607423E-5 0.0 0.0 6.399659677734083E-5 16-17 0.0 1.1635744868607423E-5 0.0 0.0 1.1635744868607424E-4 18-19 0.0 1.7453617302911137E-5 0.0 5.817872434303712E-6 1.512646832918965E-4 20-21 0.0 2.3271489737214847E-5 0.0 2.9089362171518557E-5 1.512646832918965E-4 22-23 0.0 2.3271489737214847E-5 0.0 2.443506422407559E-4 1.978076627663262E-4 24-25 0.0 2.3271489737214847E-5 0.0 9.250417170542902E-4 2.443506422407559E-4 26-27 0.0 2.3271489737214847E-5 0.0 0.0020886162039150326 2.6762213197797073E-4 28-29 0.0 2.3271489737214847E-5 0.0 0.005183724338964607 2.6762213197797073E-4 30-31 0.0 2.3271489737214847E-5 0.0 0.015271915140047242 2.6762213197797073E-4 32-33 0.0 2.3271489737214847E-5 0.0 0.03885175211628018 2.967114941494893E-4 34-35 0.0 2.3271489737214847E-5 0.0 0.07702863103018115 3.2580085632100787E-4 36-37 0.0 4.0725107040125984E-5 0.0 0.136964352848378 3.2580085632100787E-4 38-39 0.0 5.817872434303712E-5 0.0 0.21996793886858904 3.5489021849252644E-4 40-41 0.0 7.563234164594825E-5 0.0 0.3063168015385247 3.6070809092683014E-4 42-43 0.0 9.890383138316309E-5 0.0 0.3946902838155981 3.6070809092683014E-4 44-45 0.0 1.0472170381746681E-4 0.0 0.5037812098312271 3.7234383579543755E-4 46-47 0.0 1.0472170381746681E-4 0.0 0.6347996970517467 4.1888681526986723E-4 48-49 0.0 1.1635744868607423E-4 0.0 0.7761332720982866 4.305225601384747E-4 50-51 0.0 1.2799319355468166E-4 0.0 0.9246868268357976 4.305225601384747E-4 52-53 0.0 1.2799319355468166E-4 0.0 1.0710295900482731 4.5961192230999326E-4 54-55 0.0 1.2799319355468166E-4 0.0 1.2344885339624703 4.945191569158155E-4 56-57 0.0 1.2799319355468166E-4 0.0 1.4269670255789744 5.410621363902452E-4 58-59 0.0 1.2799319355468166E-4 0.0 1.6434907839664556 5.817872434303712E-4 60-61 0.0 1.396289384232891E-4 0.0 1.8665363773527912 6.05058733167586E-4 62-63 0.0 1.396289384232891E-4 0.0 2.089087451582211 6.166944780361934E-4 64-65 0.0 1.454468108575928E-4 0.0 2.3193064816800435 6.166944780361934E-4 66-67 0.0 1.512646832918965E-4 0.0 2.577800371808592 6.283302229048008E-4 68-69 0.0 1.512646832918965E-4 0.0 2.8611889380835254 6.516017126420157E-4 70-71 0.0 1.512646832918965E-4 0.0 3.169361640928593 6.632374575106231E-4 72-73 0.0 1.512646832918965E-4 0.0 3.479518238273758 8.377736305397345E-4 74-75 0.0 1.512646832918965E-4 0.0 3.7847121904324625 8.377736305397345E-4 76-77 0.0 1.6290042816050393E-4 0.0 4.1160051183314526 8.435915029740382E-4 78-79 0.0 1.6290042816050393E-4 0.0 4.476864473941575 8.49409375408342E-4 80-81 0.0 1.7453617302911134E-4 0.0 4.865533259787668 8.843166100141642E-4 82-83 0.0 1.8035404546341507E-4 0.0 5.259601031252796 9.424953343572013E-4 84-85 0.0 1.8617191789771877E-4 0.0 5.646344101323136 9.424953343572013E-4 86-87 0.0 1.8617191789771877E-4 0.0 6.046031937559801 9.424953343572013E-4 88 0.0 1.8617191789771877E-4 0.0 6.365322594626822 9.541310792258087E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15790 0.0 53.828056 1 GTATCAA 28655 0.0 42.12529 1 CAACGCA 36190 0.0 32.49872 5 ATCAACG 36370 0.0 32.358685 3 TATCAAC 37485 0.0 31.944933 2 AACGCAG 38750 0.0 30.666483 6 TCAACGC 38575 0.0 30.61868 4 GTACTAG 3640 0.0 29.65506 1 GTGGTAT 6935 0.0 27.543573 1 ACGCAGA 44015 0.0 26.77438 7 CGCAGAG 45370 0.0 25.912706 8 GTACATG 39325 0.0 24.143461 1 GCAGAGT 49195 0.0 23.629692 9 TACATGG 40340 0.0 23.298283 2 TGGTATC 7900 0.0 22.985962 2 ACATGGG 40735 0.0 22.371141 3 GAGTACT 26095 0.0 21.858286 12-13 TAGGCAT 5235 0.0 21.686985 5 CTAGGCA 5560 0.0 21.521599 4 TAGTACT 4825 0.0 21.493351 4 >>END_MODULE