FastQCFastQC Report
Mon 6 Jun 2016
ERR1211304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4159326
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162860.3915538238647319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134260.32279268323762067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70760.1701237171599437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78550.052.1015471
GTATCAA142950.041.167141
ATCAACG173350.033.072273
CAACGCA174200.033.044985
TATCAAC176600.032.9226532
TCAACGC181100.031.916074
AACGCAG185200.031.3609926
TACCTGG32050.030.8218272
GTGGTAT34250.028.1559931
ACGCAGA207150.027.6989927
CGCAGAG211400.027.0963658
GTACATG186350.025.6977981
TACATGG192250.024.443662
GCAGAGT232000.024.1646469
ACCTGGG40200.023.9638043
TGGTATC40050.023.725532
ACATGGG192100.023.5573753
GTACCTG44850.022.655251
GAGTACT127650.022.01341812-13
CATGGGG131700.021.9438134
Produced by FastQC (version 0.11.5)