##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4159326 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.484179407913686 31.0 31.0 34.0 29.0 34.0 2 31.496888678598406 31.0 31.0 34.0 28.0 34.0 3 31.5460168306115 33.0 31.0 34.0 28.0 34.0 4 35.15651694529354 37.0 35.0 37.0 32.0 37.0 5 35.204046761422404 37.0 35.0 37.0 32.0 37.0 6 35.269603296303295 37.0 35.0 37.0 32.0 37.0 7 35.2049586880182 37.0 35.0 37.0 32.0 37.0 8 35.20277996963931 37.0 35.0 37.0 32.0 37.0 9 36.76962204934165 39.0 37.0 39.0 32.0 39.0 10-11 36.70257344579386 39.0 37.0 39.0 32.0 39.0 12-13 36.62483621144388 39.0 37.0 39.0 32.0 39.0 14-15 37.773274564196214 40.0 37.0 41.0 32.0 41.0 16-17 37.76713498773599 40.0 37.0 41.0 32.0 41.0 18-19 37.76960269524437 40.0 37.5 41.0 32.0 41.0 20-21 37.735587208119775 40.0 37.0 41.0 32.0 41.0 22-23 37.61325500814315 40.0 37.0 41.0 32.0 41.0 24-25 37.50486256667547 40.0 37.0 41.0 31.5 41.0 26-27 37.36165174357576 40.0 37.0 41.0 31.0 41.0 28-29 37.22326970764013 40.0 36.0 41.0 31.0 41.0 30-31 37.03373875959711 39.5 36.0 41.0 30.0 41.0 32-33 36.900427497147376 39.0 36.0 41.0 30.0 41.0 34-35 36.75081924331009 39.0 35.0 41.0 30.0 41.0 36-37 36.663946153775875 39.0 35.0 41.0 30.0 41.0 38-39 36.512880933112726 39.0 35.0 41.0 30.0 41.0 40-41 36.338717859576285 39.0 35.0 40.5 29.0 41.0 42-43 36.185581149445845 39.0 35.0 40.0 28.0 41.0 44-45 36.04817727679917 39.0 35.0 40.0 28.0 41.0 46-47 35.868619218594546 39.0 35.0 40.0 27.0 41.0 48-49 35.627075035714924 38.5 34.5 40.0 26.0 41.0 50-51 34.944272942298824 37.5 33.5 39.5 25.0 40.5 52-53 35.168529588688166 38.0 34.0 40.0 26.0 40.5 54-55 35.51828012519336 38.0 34.0 40.0 26.0 41.0 56-57 35.414691707262186 38.0 34.0 40.0 26.0 41.0 58-59 35.18909962816091 38.0 34.0 40.0 26.0 41.0 60-61 34.920857970738524 37.5 34.0 40.0 26.0 41.0 62-63 34.64053935661691 37.0 33.5 40.0 26.0 41.0 64-65 34.35684170945004 36.5 33.0 40.0 25.0 41.0 66-67 34.12996660997479 36.0 33.0 39.0 25.0 41.0 68-69 33.7887520718501 35.5 33.0 39.0 24.5 41.0 70-71 33.44582355410468 35.0 32.5 38.5 24.0 40.5 72-73 33.07814847886412 35.0 32.0 37.5 23.5 40.0 74-75 32.70501025887367 35.0 32.0 37.0 23.0 39.0 76-77 32.3533199850168 35.0 31.5 36.5 22.0 39.0 78-79 31.996006925160472 35.0 31.0 36.0 21.0 39.0 80-81 31.669247974311222 35.0 31.0 36.0 20.0 37.5 82-83 31.364093725762302 34.5 31.0 35.0 20.0 37.0 84-85 31.067224473388237 34.0 30.0 35.0 20.0 36.5 86-87 30.811475465015246 34.0 30.0 35.0 19.0 36.0 88-89 30.55866178799161 34.0 30.0 35.0 18.0 36.0 90-91 30.307307842664894 34.0 29.0 35.0 18.0 35.5 92-93 30.069555500097852 34.0 29.0 35.0 17.5 35.0 94-95 29.846147669117542 34.0 29.0 35.0 16.5 35.0 96-97 29.638920584729355 34.0 29.0 35.0 15.0 35.0 98-99 29.39737916672076 34.0 28.0 35.0 13.5 35.0 100 27.701902664037394 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3404107184804559 1101 2 -0.2861839755946711 1101 3 -0.36453086441866844 1101 4 -0.24896947500453592 1101 5 -0.274773077453041 1101 6 -0.34725488779202607 1101 7 -0.43492559833560307 1101 8 -0.4761333504147771 1101 9 -0.4658948118945645 1101 10-11 -0.5439056691638839 1101 12-13 -0.5355602612521935 1101 14-15 -0.5516192796262018 1101 16-17 -0.6649713547480971 1101 18-19 -0.600286700531754 1101 20-21 -0.6763189824497005 1101 22-23 -0.6261933954737131 1101 24-25 -0.6719091273881901 1101 26-27 -0.8224476857224801 1101 28-29 -0.7449018250323078 1101 30-31 -0.7940976834081326 1101 32-33 -0.8688243551948034 1101 34-35 -0.820245704538813 1101 36-37 -0.9044999654907429 1101 38-39 -0.9461521787032936 1101 40-41 -1.0140083739447903 1101 42-43 -1.0966745423590183 1101 44-45 -0.9837925400651528 1101 46-47 -0.9175878014522283 1101 48-49 -0.9650367664182156 1101 50-51 -0.9423297971465772 1101 52-53 -0.77996716463079 1101 54-55 -0.7487659903888328 1101 56-57 -0.5552941113812935 1101 58-59 -0.6679539588983872 1101 60-61 -0.7144455106287992 1101 62-63 -0.8116649446091984 1101 64-65 -0.780809786543351 1101 66-67 -0.7641774647071529 1101 68-69 -0.8032468349238613 1101 70-71 -0.8565669481282967 1101 72-73 -0.8804523431538556 1101 74-75 -0.8321299433727809 1101 76-77 -0.805978749853324 1101 78-79 -0.9247862214513525 1101 80-81 -0.9940100955089761 1101 82-83 -1.0440912405055975 1101 84-85 -0.8147703025919775 1101 86-87 -1.0335453241845372 1101 88-89 -1.0432224996150232 1101 90-91 -0.9775985886382514 1101 92-93 -1.107948130907861 1101 94-95 -1.163196992918266 1101 96-97 -1.1248803486667072 1101 98-99 -1.2971605632796646 1101 100 -1.1878301781519802 1102 1 -0.32651752087902963 1102 2 -0.3015997062441258 1102 3 -0.35023033476001686 1102 4 -0.2050059489671625 1102 5 -0.18685905733090635 1102 6 -0.22710629947328442 1102 7 -0.32234349536339835 1102 8 -0.2590671329918166 1102 9 -0.42319892727827124 1102 10-11 -0.36035399159069925 1102 12-13 -0.38956540132162587 1102 14-15 -0.5129955126327062 1102 16-17 -0.5262793003229191 1102 18-19 -0.4960530000627017 1102 20-21 -0.46089713012403877 1102 22-23 -0.5445239863050233 1102 24-25 -0.6201282342089058 1102 26-27 -0.6639637709613382 1102 28-29 -0.7074840148841943 1102 30-31 -0.6634727083860383 1102 32-33 -0.6826895107074336 1102 34-35 -0.6648239644884484 1102 36-37 -0.7643793083221198 1102 38-39 -0.8086491730003829 1102 40-41 -0.8131997513099094 1102 42-43 -0.7962227746849138 1102 44-45 -0.6850073586383161 1102 46-47 -0.7617707588393472 1102 48-49 -0.8858228110281274 1102 50-51 -0.9185109297930083 1102 52-53 -0.8108837346048148 1102 54-55 -0.6741303604422839 1102 56-57 -0.558355818278379 1102 58-59 -0.6530622798201762 1102 60-61 -0.7651797375219118 1102 62-63 -0.7912212527358875 1102 64-65 -0.9544347584912387 1102 66-67 -0.8418823199849825 1102 68-69 -0.7193144104767697 1102 70-71 -0.814143431321547 1102 72-73 -0.7403921676114535 1102 74-75 -0.6846798487340706 1102 76-77 -0.748660412933642 1102 78-79 -0.7612717390758341 1102 80-81 -0.9284833326232835 1102 82-83 -0.8590835262579581 1102 84-85 -0.8939282874163688 1102 86-87 -0.9543807565533164 1102 88-89 -1.0714753384755262 1102 90-91 -1.075553828492339 1102 92-93 -1.06755248617306 1102 94-95 -0.9700369569313629 1102 96-97 -0.9773975070701582 1102 98-99 -1.0468520401554322 1102 100 -1.4102260632093575 1103 1 -0.32823067272948947 1103 2 -0.38775556201395744 1103 3 -0.4160017596062566 1103 4 -0.21265193644673985 1103 5 -0.2728344211618037 1103 6 -0.2497598298793804 1103 7 -0.3651037195656315 1103 8 -0.31810003432568834 1103 9 -0.4945281378123454 1103 10-11 -0.4267467577388757 1103 12-13 -0.560031652101685 1103 14-15 -0.6385716802309318 1103 16-17 -0.6241528969964136 1103 18-19 -0.6102903651536025 1103 20-21 -0.7337679406349622 1103 22-23 -0.6675365124050003 1103 24-25 -0.6921070196097361 1103 26-27 -0.6551943581031665 1103 28-29 -0.6129251063457914 1103 30-31 -0.5983155918100209 1103 32-33 -0.6796991535349122 1103 34-35 -0.6934937801499785 1103 36-37 -0.8495766768321502 1103 38-39 -0.7888755000184986 1103 40-41 -0.634359628080368 1103 42-43 -0.7663796261697442 1103 44-45 -0.8834488793853552 1103 46-47 -0.9197748831714421 1103 48-49 -0.7604178368893955 1103 50-51 -0.8016240390665743 1103 52-53 -0.729728026258158 1103 54-55 -0.7304677171205896 1103 56-57 -0.7430389369194046 1103 58-59 -0.6317074933957656 1103 60-61 -0.6678203974799715 1103 62-63 -0.7510472906964054 1103 64-65 -0.7131692428793954 1103 66-67 -0.8322025690841457 1103 68-69 -0.7666896559904401 1103 70-71 -0.7636603296680065 1103 72-73 -0.9584515864504013 1103 74-75 -0.9058077995980689 1103 76-77 -0.7806912738768119 1103 78-79 -0.7914313063929015 1103 80-81 -0.7277058309671389 1103 82-83 -0.7507159361993097 1103 84-85 -0.7995711051626557 1103 86-87 -0.9752642960401374 1103 88-89 -1.0269420802152993 1103 90-91 -0.9831206876025931 1103 92-93 -1.0853482204229188 1103 94-95 -1.1885892289928357 1103 96-97 -1.3146722235104917 1103 98-99 -1.3451231180838583 1103 100 -1.2713077272571205 1104 1 -0.38143855840470664 1104 2 -0.29498107214213576 1104 3 -0.4147867754914145 1104 4 -0.2828736948801307 1104 5 -0.15121161109380665 1104 6 -0.265638674654916 1104 7 -0.3415310271559946 1104 8 -0.3457257171752275 1104 9 -0.39973742416324853 1104 10-11 -0.42802243063920997 1104 12-13 -0.4887643212240107 1104 14-15 -0.5307969567341431 1104 16-17 -0.5610327670364725 1104 18-19 -0.6179570572415258 1104 20-21 -0.5441251532151625 1104 22-23 -0.5891400954064565 1104 24-25 -0.5175810768994111 1104 26-27 -0.5477846400562854 1104 28-29 -0.6183925348626005 1104 30-31 -0.6856760243867939 1104 32-33 -0.7321896829962355 1104 34-35 -0.7847585022154817 1104 36-37 -0.7062571224489602 1104 38-39 -0.7413588998901943 1104 40-41 -0.7199044992469439 1104 42-43 -0.7742479204953128 1104 44-45 -0.8002877213896227 1104 46-47 -0.8476819431300839 1104 48-49 -0.8295871416832483 1104 50-51 -0.8570964757508506 1104 52-53 -0.7609660345166631 1104 54-55 -0.7000576005685843 1104 56-57 -0.6582312935159678 1104 58-59 -0.7705970685393595 1104 60-61 -0.6319017666404534 1104 62-63 -0.6289170847868704 1104 64-65 -0.5939135639800028 1104 66-67 -0.6725447410498475 1104 68-69 -0.6108919435279958 1104 70-71 -0.6321963362438723 1104 72-73 -0.5986264346008809 1104 74-75 -0.7494078650667646 1104 76-77 -0.6797148020596353 1104 78-79 -0.603410299211486 1104 80-81 -0.6366687589808393 1104 82-83 -0.754215275738833 1104 84-85 -0.7092849897631979 1104 86-87 -0.7024362160909874 1104 88-89 -0.7646653901064724 1104 90-91 -0.6401207294939653 1104 92-93 -0.8741848137197117 1104 94-95 -0.8247822483324185 1104 96-97 -0.8400404043802574 1104 98-99 -0.947503823668864 1104 100 -0.9737194619977174 1105 1 -0.38829797699694524 1105 2 -0.45146210322606706 1105 3 -0.3941462115392511 1105 4 -0.2804882293758908 1105 5 -0.2537438033740429 1105 6 -0.28399152445597764 1105 7 -0.243461815873097 1105 8 -0.3193307239064396 1105 9 -0.39690735624600393 1105 10-11 -0.47076623797462247 1105 12-13 -0.4871713399041582 1105 14-15 -0.5480090083455238 1105 16-17 -0.6105624467079522 1105 18-19 -0.539176349102803 1105 20-21 -0.5081617321704925 1105 22-23 -0.5891825208236696 1105 24-25 -0.6406372297319933 1105 26-27 -0.5781737735636909 1105 28-29 -0.6623634209187657 1105 30-31 -0.6715064337687977 1105 32-33 -0.589231155171511 1105 34-35 -0.6237334961558929 1105 36-37 -0.735396867317931 1105 38-39 -0.7220380789517264 1105 40-41 -0.8716416638126745 1105 42-43 -0.6913456628712851 1105 44-45 -0.6718782522758957 1105 46-47 -0.7935085089554832 1105 48-49 -0.7593072436505466 1105 50-51 -0.7326084318385497 1105 52-53 -0.7761013021076621 1105 54-55 -0.7485071277878461 1105 56-57 -0.8299936207429468 1105 58-59 -0.6224364397920183 1105 60-61 -0.6918233793648909 1105 62-63 -0.7002597129217705 1105 64-65 -0.7064641727481629 1105 66-67 -0.7149044587146776 1105 68-69 -0.7402777957153148 1105 70-71 -0.5836183025688655 1105 72-73 -0.6755022215574584 1105 74-75 -0.6752957482545661 1105 76-77 -0.7294196694452921 1105 78-79 -0.78671712774198 1105 80-81 -0.8192114312700944 1105 82-83 -0.7272149888020358 1105 84-85 -0.653741440704156 1105 86-87 -0.6791425008712686 1105 88-89 -0.7731576220325316 1105 90-91 -0.8250056771487806 1105 92-93 -0.9061291247073271 1105 94-95 -0.8574265454553043 1105 96-97 -0.8552290362303765 1105 98-99 -0.8101465481254273 1105 100 -1.0462336766736549 1106 1 -0.4429207760896112 1106 2 -0.503085038809111 1106 3 -0.4203445645553643 1106 4 -0.28523289487696957 1106 5 -0.18859434641075978 1106 6 -0.28513219403876633 1106 7 -0.3748386788042595 1106 8 -0.3503062401325394 1106 9 -0.4463318218211825 1106 10-11 -0.487993273468625 1106 12-13 -0.44236869849037674 1106 14-15 -0.5799484066179446 1106 16-17 -0.616075033070608 1106 18-19 -0.578784556724159 1106 20-21 -0.5855689999581131 1106 22-23 -0.5414560605391117 1106 24-25 -0.5927364192391238 1106 26-27 -0.6119606665877271 1106 28-29 -0.7450322302677037 1106 30-31 -0.7029721779338374 1106 32-33 -0.759549047760089 1106 34-35 -0.8917596503120251 1106 36-37 -0.8298394341169697 1106 38-39 -0.784217732786125 1106 40-41 -0.7453674286462544 1106 42-43 -0.6777388721500373 1106 44-45 -0.8042425580923336 1106 46-47 -0.7065971237081357 1106 48-49 -0.7241799826224451 1106 50-51 -0.7395241067834633 1106 52-53 -0.6364251742746774 1106 54-55 -0.7436414083210678 1106 56-57 -0.7046809927832172 1106 58-59 -0.5577422757305825 1106 60-61 -0.6203768413458164 1106 62-63 -0.6135254401880061 1106 64-65 -0.657693886053373 1106 66-67 -0.6371241172287583 1106 68-69 -0.5586401386701354 1106 70-71 -0.6049306250501303 1106 72-73 -0.5809200770986465 1106 74-75 -0.5581416701170738 1106 76-77 -0.6037944135258364 1106 78-79 -0.5303602509017971 1106 80-81 -0.6091485157056411 1106 82-83 -0.7477558156737452 1106 84-85 -0.7313315081044003 1106 86-87 -0.9026095839419099 1106 88-89 -0.8769418177467294 1106 90-91 -0.7471067190253287 1106 92-93 -0.7475190192197658 1106 94-95 -0.970553832481567 1106 96-97 -0.9453173975009044 1106 98-99 -0.8461039631276641 1106 100 -0.7374154233605132 1107 1 -0.5146319136808835 1107 2 -0.5022850346096135 1107 3 -0.47753226632807966 1107 4 -0.31658161586763356 1107 5 -0.47965965384019427 1107 6 -0.37256231178216836 1107 7 -0.4328389832677999 1107 8 -0.3659283693825657 1107 9 -0.5661413746272359 1107 10-11 -0.5375275229922423 1107 12-13 -0.4911329964657014 1107 14-15 -0.6065137691657796 1107 16-17 -0.6621570335010674 1107 18-19 -0.6883668904896894 1107 20-21 -0.6681009292595803 1107 22-23 -0.733074258009971 1107 24-25 -0.7735444549768431 1107 26-27 -0.6321850509676992 1107 28-29 -0.7505931518473474 1107 30-31 -0.811087049927913 1107 32-33 -0.7071034968648036 1107 34-35 -0.7683386612254708 1107 36-37 -0.8410451359841673 1107 38-39 -0.7347131422108504 1107 40-41 -0.8079635577597912 1107 42-43 -0.9832875889411952 1107 44-45 -0.9806567387442016 1107 46-47 -0.8738855346709329 1107 48-49 -0.7981634418531982 1107 50-51 -0.7536664382392715 1107 52-53 -0.7333474678300291 1107 54-55 -0.5575101162932867 1107 56-57 -0.5095849555336756 1107 58-59 -0.3727881552365133 1107 60-61 -0.4840044719370553 1107 62-63 -0.5899821407481127 1107 64-65 -0.5541723452578893 1107 66-67 -0.4481539651544679 1107 68-69 -0.44972998691081045 1107 70-71 -0.39240903701030305 1107 72-73 -0.4809438305041738 1107 74-75 -0.5183982856373603 1107 76-77 -0.5591297433700859 1107 78-79 -0.49373223710644965 1107 80-81 -0.5856427492711731 1107 82-83 -0.5781461326832833 1107 84-85 -0.5869289128151856 1107 86-87 -0.6156469227205896 1107 88-89 -0.5992016458823137 1107 90-91 -0.6343616389986622 1107 92-93 -0.6119769691302643 1107 94-95 -0.5924154957973862 1107 96-97 -0.6131468874194859 1107 98-99 -0.6697094676185031 1107 100 -0.7570884925104835 1108 1 -0.5570354497814876 1108 2 -0.46074410449154257 1108 3 -0.453951674430094 1108 4 -0.45204482815763924 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2208 56-57 0.8774601042548795 2208 58-59 0.8850892478239203 2208 60-61 0.9321990133023874 2208 62-63 0.9869809419518631 2208 64-65 0.9815191750191303 2208 66-67 0.902659530693974 2208 68-69 0.9324682618796416 2208 70-71 1.002398020928652 2208 72-73 0.9761677669920559 2208 74-75 1.091747306385919 2208 76-77 1.0601371872385954 2208 78-79 1.0419230273989264 2208 80-81 1.0565349564412827 2208 82-83 1.1351087822048989 2208 84-85 1.089950642164741 2208 86-87 1.0876719585043837 2208 88-89 1.1113139223460315 2208 90-91 1.1032451063597648 2208 92-93 1.0729239522125589 2208 94-95 1.1351195914228462 2208 96-97 1.1190218826397142 2208 98-99 1.0775512972778785 2208 100 1.2101883309038293 2209 1 0.42422220930498966 2209 2 0.32376080893258674 2209 3 0.38572928341563895 2209 4 0.33414232753550266 2209 5 0.3528059030156996 2209 6 0.4068035218616828 2209 7 0.42976959942846804 2209 8 0.38056087916491066 2209 9 0.4999291068110452 2209 10-11 0.5681694313266163 2209 12-13 0.6139802649187729 2209 14-15 0.7846634302143158 2209 16-17 0.6931358447785598 2209 18-19 0.6836850897197877 2209 20-21 0.6830811983608029 2209 22-23 0.7647932837159104 2209 24-25 0.7384903727969672 2209 26-27 0.7346700210872896 2209 28-29 0.7187695384319781 2209 30-31 0.7304549967869107 2209 32-33 0.8039479414916499 2209 34-35 0.8393381830524902 2209 36-37 0.8531348303940618 2209 38-39 0.905471495473698 2209 40-41 0.8821414381074462 2209 42-43 0.8648065108104461 2209 44-45 0.9751485806703357 2209 46-47 0.9699021391533833 2209 48-49 0.9645112693880549 2209 50-51 1.0133643889889399 2209 52-53 0.8799534152868347 2209 54-55 0.847856432182283 2209 56-57 0.7979514785181365 2209 58-59 0.796139950849458 2209 60-61 0.907239026763186 2209 62-63 0.8707069623209733 2209 64-65 0.8969730712142265 2209 66-67 0.9215308511278337 2209 68-69 0.9130208349583668 2209 70-71 0.9208577020944162 2209 72-73 0.9552309405634745 2209 74-75 0.8641709923319141 2209 76-77 0.8377852531339798 2209 78-79 0.8120915700952374 2209 80-81 0.9051502941645495 2209 82-83 0.8575951174040348 2209 84-85 0.8905711582493083 2209 86-87 0.9088839083638369 2209 88-89 0.865648696692638 2209 90-91 1.01236580782912 2209 92-93 0.9937184702477531 2209 94-95 1.0381744981891252 2209 96-97 0.9847049870636404 2209 98-99 1.0517468158162409 2209 100 1.2855261323207543 2210 1 0.6348437722693312 2210 2 0.6558011759481452 2210 3 0.6265163203373909 2210 4 0.473366039048436 2210 5 0.4583061561188373 2210 6 0.5033958518583717 2210 7 0.5564533222465613 2210 8 0.559135680835567 2210 9 0.6547762008562046 2210 10-11 0.7340083917432807 2210 12-13 0.6839196162134797 2210 14-15 0.9449574220474304 2210 16-17 0.8617319930219409 2210 18-19 0.8411123950482562 2210 20-21 0.8519511266488422 2210 22-23 0.8889565137174671 2210 24-25 0.931851409036426 2210 26-27 1.0197408579497775 2210 28-29 0.9842452033568634 2210 30-31 0.9936357472884083 2210 32-33 1.0183614714640044 2210 34-35 1.0407195027603535 2210 36-37 1.0076988064362382 2210 38-39 1.0476601643859738 2210 40-41 1.0659726432163268 2210 42-43 1.093815558669526 2210 44-45 1.0975998738797372 2210 46-47 1.111348818344183 2210 48-49 1.2156304459259815 2210 50-51 1.0392001629384922 2210 52-53 1.035965464098922 2210 54-55 0.9816867613017308 2210 56-57 1.0289085134157787 2210 58-59 1.0189800321768487 2210 60-61 1.043762311896046 2210 62-63 0.9829843943077279 2210 64-65 0.9696741681486358 2210 66-67 0.9807113403443566 2210 68-69 0.9435413736642957 2210 70-71 0.9540434052711291 2210 72-73 0.9806138457677207 2210 74-75 0.9396620933465698 2210 76-77 0.935170909389683 2210 78-79 0.9293267909530663 2210 80-81 0.8796925518943368 2210 82-83 0.9586622414942987 2210 84-85 1.064543702695321 2210 86-87 1.0129795324364856 2210 88-89 1.0189009786228524 2210 90-91 1.0349795414934704 2210 92-93 1.133388669567811 2210 94-95 1.1369212952694205 2210 96-97 1.2232857312331085 2210 98-99 1.230643132285163 2210 100 1.3539443302050742 2211 1 0.6110301509101141 2211 2 0.6795763348606592 2211 3 0.6504770829171171 2211 4 0.4937012240653331 2211 5 0.5331107847791188 2211 6 0.49563547182792433 2211 7 0.47034932122184614 2211 8 0.5312410149865698 2211 9 0.6519362411290714 2211 10-11 0.6805471561649128 2211 12-13 0.7248704937655148 2211 14-15 0.9013316602226382 2211 16-17 0.8864640171055171 2211 18-19 0.8763715460693504 2211 20-21 0.960977896153949 2211 22-23 0.9120584009176653 2211 24-25 0.8527062038186983 2211 26-27 0.8672699051473174 2211 28-29 0.9661884263453118 2211 30-31 0.9960613859618022 2211 32-33 1.0031762291649073 2211 34-35 0.9996359297672228 2211 36-37 1.0243733504505101 2211 38-39 1.0218432309628653 2211 40-41 1.0937835414343127 2211 42-43 1.043093654324018 2211 44-45 1.1359859770936254 2211 46-47 1.1700845523543535 2211 48-49 1.2220593097202581 2211 50-51 1.1490672474564079 2211 52-53 1.0910778120112496 2211 54-55 1.012053041865066 2211 56-57 1.0286486235579204 2211 58-59 1.0588421094208869 2211 60-61 1.052824914089875 2211 62-63 1.0682072475128521 2211 64-65 1.0754469252155232 2211 66-67 1.0516738836459183 2211 68-69 1.0863002008852547 2211 70-71 1.0515014758373766 2211 72-73 1.1620577993637653 2211 74-75 1.0814047516705187 2211 76-77 1.0873075966960712 2211 78-79 1.0548852120105998 2211 80-81 1.0548596130843322 2211 82-83 1.0332382422155924 2211 84-85 1.1258899187419225 2211 86-87 1.1239020533260664 2211 88-89 1.1201170985741662 2211 90-91 1.2611559914561568 2211 92-93 1.256160531085829 2211 94-95 1.2481054502441005 2211 96-97 1.2473333473843304 2211 98-99 1.329348548942587 2211 100 1.4075972071603076 2212 1 0.45516009325165285 2212 2 0.4047007330386556 2212 3 0.44452724416296974 2212 4 0.3590419063559267 2212 5 0.307327916335673 2212 6 0.37257361403155187 2212 7 0.4342607836729968 2212 8 0.4583300555298919 2212 9 0.5796653368684872 2212 10-11 0.6167796294034318 2212 12-13 0.6155965245358672 2212 14-15 0.7158825728033236 2212 16-17 0.7406103541202285 2212 18-19 0.7654186925052784 2212 20-21 0.671436138493462 2212 22-23 0.795786705335253 2212 24-25 0.8050361222381852 2212 26-27 0.7679665559898794 2212 28-29 0.7701158169545366 2212 30-31 0.812486689529841 2212 32-33 0.8045401544298869 2212 34-35 0.739531318142781 2212 36-37 0.7957964336873218 2212 38-39 0.8248344783680892 2212 40-41 0.9079885576290536 2212 42-43 0.9136629447931526 2212 44-45 0.8759174940814347 2212 46-47 0.9001320877867016 2212 48-49 0.9712228271177636 2212 50-51 0.9358884855383494 2212 52-53 0.8493133379421209 2212 54-55 0.83539530855316 2212 56-57 0.7896627642265983 2212 58-59 0.7958344936497284 2212 60-61 0.8241274663132572 2212 62-63 0.8039025093330423 2212 64-65 0.8083697983451756 2212 66-67 0.8482316073469178 2212 68-69 0.9039078395963358 2212 70-71 0.8892508158763661 2212 72-73 0.8635206749347191 2212 74-75 0.8764612526109232 2212 76-77 0.8916443279896953 2212 78-79 0.7652106904691713 2212 80-81 0.7803698946560047 2212 82-83 0.9270781296767261 2212 84-85 0.8228696018343662 2212 86-87 0.8127561309134883 2212 88-89 0.8849539884127644 2212 90-91 0.9095613253722235 2212 92-93 0.8956426239216277 2212 94-95 0.946590609101257 2212 96-97 1.0144136749691235 2212 98-99 0.9833518885592589 2212 100 1.1602093409021172 2213 1 0.47897695842006627 2213 2 0.5677157345402115 2213 3 0.5424521055617753 2213 4 0.3807158790283012 2213 5 0.39725323656144695 2213 6 0.4711266871355235 2213 7 0.49317799550323826 2213 8 0.42077053677650866 2213 9 0.6056418992378596 2213 10-11 0.550840977110667 2213 12-13 0.6622428739519961 2213 14-15 0.8202613187476189 2213 16-17 0.743318837804118 2213 18-19 0.7429520979177013 2213 20-21 0.7202327794921217 2213 22-23 0.7676467671230611 2213 24-25 0.7465755218303229 2213 26-27 0.7866692729010012 2213 28-29 0.8463046704021266 2213 30-31 0.8598333603923933 2213 32-33 0.9420729528663969 2213 34-35 0.9117601470571799 2213 36-37 0.9128921609782381 2213 38-39 0.8729063433124438 2213 40-41 0.9299215401314243 2213 42-43 0.9846134540175129 2213 44-45 0.9676267047987182 2213 46-47 0.9645457758124536 2213 48-49 1.050667027805737 2213 50-51 0.9778969454113877 2213 52-53 0.8708009121587281 2213 54-55 0.8915159583083252 2213 56-57 0.8927613846021245 2213 58-59 0.949264542936838 2213 60-61 0.8653770808919106 2213 62-63 0.8124057736260752 2213 64-65 0.7676570564661063 2213 66-67 0.7737291185784798 2213 68-69 0.8780927612446021 2213 70-71 0.809823378090563 2213 72-73 0.8129790618034392 2213 74-75 0.8553721839962236 2213 76-77 0.8663764058137033 2213 78-79 0.9281149564898321 2213 80-81 0.8485406883129514 2213 82-83 0.8818333010111452 2213 84-85 0.8728210437616575 2213 86-87 0.86294551408551 2213 88-89 0.9367920301847477 2213 90-91 0.9254161464330508 2213 92-93 0.9336639376382116 2213 94-95 0.9375289436931347 2213 96-97 0.979167520320356 2213 98-99 1.0190330844121576 2213 100 1.1234886361010865 2214 1 0.431332665168906 2214 2 0.4310049348747711 2214 3 0.4021106086612285 2214 4 0.28450294617866945 2214 5 0.31222901964103755 2214 6 0.35649133997055316 2214 7 0.34692242239846394 2214 8 0.3468526169247923 2214 9 0.46382732209430344 2214 10-11 0.5209843474379383 2214 12-13 0.4677276692202028 2214 14-15 0.577426446315279 2214 16-17 0.6200800075260489 2214 18-19 0.6404120741475978 2214 20-21 0.6457316939060362 2214 22-23 0.6748771854895566 2214 24-25 0.6750342355873613 2214 26-27 0.7001558684983493 2214 28-29 0.6751638310451042 2214 30-31 0.680257013417183 2214 32-33 0.6915404935578877 2214 34-35 0.7031352099381678 2214 36-37 0.7213947834878027 2214 38-39 0.7984539559769317 2214 40-41 0.8088430462494998 2214 42-43 0.8421393586419086 2214 44-45 0.8798999023191669 2214 46-47 0.8113400335691239 2214 48-49 0.7038463709115206 2214 50-51 0.6957725419649634 2214 52-53 0.5787140766513801 2214 54-55 0.5329240473053005 2214 56-57 0.4525668526626063 2214 58-59 0.5080053015652126 2214 60-61 0.5436707895799842 2214 62-63 0.5008906371722333 2214 64-65 0.5294997681453495 2214 66-67 0.5083961145858922 2214 68-69 0.5546161379110117 2214 70-71 0.5649562322732677 2214 72-73 0.5604017361774822 2214 74-75 0.5751999779453882 2214 76-77 0.5930722847367988 2214 78-79 0.6174488179732656 2214 80-81 0.5618157226443827 2214 82-83 0.6298734056103008 2214 84-85 0.6505433178059583 2214 86-87 0.7160625953901665 2214 88-89 0.6902439998197956 2214 90-91 0.7038087326515452 2214 92-93 0.8228776349332314 2214 94-95 0.8282242141391194 2214 96-97 0.8888669483585652 2214 98-99 0.8802091404697023 2214 100 0.9079859078276193 2215 1 0.30081730193139933 2215 2 0.0736394227137751 2215 3 0.32505382003885686 2215 4 0.2047814345107497 2215 5 0.13619784678334668 2215 6 0.19445236622495088 2215 7 0.2574041311876485 2215 8 0.3173460620303672 2215 9 0.36439558126622984 2215 10-11 0.33050176238553064 2215 12-13 0.36339834309961816 2215 14-15 0.48449425588661654 2215 16-17 0.49573658610928106 2215 18-19 0.44174472033215295 2215 20-21 0.48034899625958616 2215 22-23 0.46368702366469705 2215 24-25 0.4053630958527563 2215 26-27 0.5789959146515073 2215 28-29 0.5099109226680767 2215 30-31 0.6316852770809973 2215 32-33 0.6320777212953956 2215 34-35 0.644497791815553 2215 36-37 0.7425183996396285 2215 38-39 0.7883235652047915 2215 40-41 0.7492136643352083 2215 42-43 0.6782503721154001 2215 44-45 0.7331230080105371 2215 46-47 0.7979272449447592 2215 48-49 0.7552242329381826 2215 50-51 0.5823961487930802 2215 52-53 0.6859045837008395 2215 54-55 0.7708034787672347 2215 56-57 0.7274641145133174 2215 58-59 0.6614063786263813 2215 60-61 0.740396894451365 2215 62-63 0.7160901363985275 2215 64-65 0.7964109056488766 2215 66-67 0.7369703237665917 2215 68-69 0.7780792242090726 2215 70-71 0.7018694927760905 2215 72-73 0.752824889461003 2215 74-75 0.799365993190392 2215 76-77 0.7472729519840087 2215 78-79 0.7128065949248317 2215 80-81 0.7669401751242617 2215 82-83 0.8201161321717798 2215 84-85 0.6722609900878034 2215 86-87 0.7540890979794348 2215 88-89 0.78938522061123 2215 90-91 0.9006289615592209 2215 92-93 1.0109365724632582 2215 94-95 1.0543183178777902 2215 96-97 1.2012596329303769 2215 98-99 1.107580612375731 2215 100 1.155395898185425 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0.4629092372713757 2311 10-11 0.4916273104726727 2311 12-13 0.4999455044813246 2311 14-15 0.6857294313327955 2311 16-17 0.6296058945165797 2311 18-19 0.48288676252884244 2311 20-21 0.5858171589057761 2311 22-23 0.6537000640123907 2311 24-25 0.6343178626270998 2311 26-27 0.7579685763860624 2311 28-29 0.7694460345750613 2311 30-31 0.7369529418983731 2311 32-33 0.7528547546685473 2311 34-35 0.770317455699498 2311 36-37 0.8073780654097931 2311 38-39 0.7985256141604609 2311 40-41 0.8028491222315708 2311 42-43 0.911181952652349 2311 44-45 0.9180262685638354 2311 46-47 0.8492530049904445 2311 48-49 0.8305033731578888 2311 50-51 0.8532035526428601 2311 52-53 0.8866200323284374 2311 54-55 0.7227259951441027 2311 56-57 0.8174398794516193 2311 58-59 0.8348815436257766 2311 60-61 0.7554610049599972 2311 62-63 0.7161926740023503 2311 64-65 0.7328622702648175 2311 66-67 0.6995521519699608 2311 68-69 0.748001872552642 2311 70-71 0.7368851020696994 2311 72-73 0.7209947903624752 2311 74-75 0.6264111811604494 2311 76-77 0.687821955890243 2311 78-79 0.6985843118820121 2311 80-81 0.7303846880950431 2311 82-83 0.7311593738058271 2311 84-85 0.7738753452314278 2311 86-87 0.81282189901831 2311 88-89 0.7737819078326318 2311 90-91 0.7622275731106782 2311 92-93 0.7217627599756646 2311 94-95 0.725816551830043 2311 96-97 0.7274019286102202 2311 98-99 0.8494942840475979 2311 100 1.097052843679812 2312 1 0.4660763851984342 2312 2 0.4159923080929637 2312 3 0.46567822678220594 2312 4 0.33275105696753116 2312 5 0.3436197937507828 2312 6 0.3039117899991268 2312 7 0.4097074417146729 2312 8 0.37868337479336844 2312 9 0.5069598373524471 2312 10-11 0.5079982181183951 2312 12-13 0.545070690651464 2312 14-15 0.6988721322849827 2312 16-17 0.6266758255487375 2312 18-19 0.6250537172330581 2312 20-21 0.6611598065141067 2312 22-23 0.7697140448341386 2312 24-25 0.7514236176983289 2312 26-27 0.6865558223238608 2312 28-29 0.693803153123902 2312 30-31 0.7160731465762851 2312 32-33 0.7295005629513369 2312 34-35 0.8369983883840462 2312 36-37 0.7645864631443899 2312 38-39 0.7422551869168288 2312 40-41 0.7883375869263958 2312 42-43 0.816364373999285 2312 44-45 0.6880329845231046 2312 46-47 0.7648879705717349 2312 48-49 0.7621531859560022 2312 50-51 0.8012199876916952 2312 52-53 0.8086347167002472 2312 54-55 0.6989637360031082 2312 56-57 0.6222515489176104 2312 58-59 0.6165266704376009 2312 60-61 0.7327958302318649 2312 62-63 0.6740576222007704 2312 64-65 0.6382517646934787 2312 66-67 0.5768977186775857 2312 68-69 0.7173466254078562 2312 70-71 0.6844414161194621 2312 72-73 0.6775697182117923 2312 74-75 0.6196780104423283 2312 76-77 0.6785481747660498 2312 78-79 0.670910605025572 2312 80-81 0.7299389371433875 2312 82-83 0.7412087881367277 2312 84-85 0.685935732409412 2312 86-87 0.7853380537929873 2312 88-89 0.8121954201302692 2312 90-91 0.8319393281261966 2312 92-93 0.8587882887258722 2312 94-95 0.9579945022988845 2312 96-97 0.8698064665454233 2312 98-99 0.8747333741539016 2312 100 1.0529172344042408 2313 1 0.45727269237235646 2313 2 0.42398328334901336 2313 3 0.45344302417557714 2313 4 0.4133316974016097 2313 5 0.3933060910844972 2313 6 0.3865307689360691 2313 7 0.39336178658893317 2313 8 0.4451505645594409 2313 9 0.5115116140931875 2313 10-11 0.5790812802979772 2313 12-13 0.6158272562016549 2313 14-15 0.7744980010147557 2313 16-17 0.7997986266792907 2313 18-19 0.7499157948326172 2313 20-21 0.6861165699668419 2313 22-23 0.6716252400392548 2313 24-25 0.6856867433182003 2313 26-27 0.7775186574056718 2313 28-29 0.7558957502956574 2313 30-31 0.7923480497817579 2313 32-33 0.8746992524431079 2313 34-35 0.8439772881617955 2313 36-37 0.8332519674391179 2313 38-39 0.8293036550988901 2313 40-41 0.8679533826813781 2313 42-43 0.7261947371697985 2313 44-45 0.8231464567470184 2313 46-47 0.9119611781732075 2313 48-49 0.9684085986765254 2313 50-51 0.9130967762664497 2313 52-53 0.8481107632937963 2313 54-55 0.7956511544733402 2313 56-57 0.8472894193687424 2313 58-59 0.7958434110236752 2313 60-61 0.7738112484791273 2313 62-63 0.7130945642371884 2313 64-65 0.7660097950849405 2313 66-67 0.7918165734643452 2313 68-69 0.7749199856627413 2313 70-71 0.7736049042036868 2313 72-73 0.8569838952043725 2313 74-75 0.7995740605791042 2313 76-77 0.7682291650421362 2313 78-79 0.790874569573365 2313 80-81 0.8102613413521595 2313 82-83 0.7931733994535684 2313 84-85 0.8003817940815772 2313 86-87 0.8234204485629277 2313 88-89 0.7897529873578435 2313 90-91 0.8215429459321868 2313 92-93 0.8526122667683076 2313 94-95 0.9876990136960409 2313 96-97 0.958604053234648 2313 98-99 1.0360926628251157 2313 100 1.2501527315168701 2314 1 0.2731581598045807 2314 2 0.14660221054628053 2314 3 0.24685106877814889 2314 4 0.1620984969979702 2314 5 0.08119318770382478 2314 6 0.21989980772684703 2314 7 0.21485589139238215 2314 8 0.270002634772581 2314 9 0.38177321071887604 2314 10-11 0.37799388276136625 2314 12-13 0.36712740154676027 2314 14-15 0.43709085139325765 2314 16-17 0.5005548499574175 2314 18-19 0.47551455347065286 2314 20-21 0.46170613986457454 2314 22-23 0.5273708823098744 2314 24-25 0.57657620221417 2314 26-27 0.5499432366049106 2314 28-29 0.48780026242491203 2314 30-31 0.5313581265808622 2314 32-33 0.6844696634488798 2314 34-35 0.6538082800969249 2314 36-37 0.6264785595418871 2314 38-39 0.7800970687706226 2314 40-41 0.7705434239626001 2314 42-43 0.5734155060898445 2314 44-45 0.7846589658269068 2314 46-47 0.5654158654021089 2314 48-49 0.4430720373376076 2314 50-51 0.5927472111294492 2314 52-53 0.5819498681162187 2314 54-55 0.5125348786128399 2314 56-57 0.5091684508866834 2314 58-59 0.5533851338220401 2314 60-61 0.3833893546697098 2314 62-63 0.541817293607906 2314 64-65 0.592902547189702 2314 66-67 0.5935983852070379 2314 68-69 0.5884658412729138 2314 70-71 0.6167231852862542 2314 72-73 0.6265699240317772 2314 74-75 0.7024532289791239 2314 76-77 0.6633265310780061 2314 78-79 0.6153573261150704 2314 80-81 0.559775320630493 2314 82-83 0.5906368828637021 2314 84-85 0.6600386465483687 2314 86-87 0.7197040441270559 2314 88-89 0.6225891908606407 2314 90-91 0.6519022759200617 2314 92-93 0.8106761511781961 2314 94-95 0.8493183488599776 2314 96-97 0.9263465059070519 2314 98-99 0.9103079633840068 2314 100 0.9321791312255101 2315 1 0.3992394747216004 2315 2 0.37101925207113595 2315 3 0.41063403800760057 2315 4 0.3233151090556703 2315 5 0.3769045543913805 2315 6 0.2872929191511844 2315 7 0.2901106230679602 2315 8 0.310852833418771 2315 9 0.21717480923231847 2315 10-11 0.3206618020405756 2315 12-13 0.34655282173611823 2315 14-15 0.5649734469545464 2315 16-17 0.47913195791216623 2315 18-19 0.5902079366616633 2315 20-21 0.41878243110889457 2315 22-23 0.3459904036726016 2315 24-25 0.5034660736825458 2315 26-27 0.6124053430919574 2315 28-29 0.5572803048074633 2315 30-31 0.6356610402052496 2315 32-33 0.6508791963504592 2315 34-35 0.5802600677363401 2315 36-37 0.6548622001223023 2315 38-39 0.6927673636943226 2315 40-41 0.67465379578843 2315 42-43 0.7377272214990924 2315 44-45 0.7632931289168781 2315 46-47 0.7742909690886322 2315 48-49 0.5800204933449962 2315 50-51 0.6298205096787655 2315 52-53 0.3174078826768536 2315 54-55 0.47481277348330053 2315 56-57 0.49411991104857833 2315 58-59 0.4749912457955574 2315 60-61 0.5904911873978946 2315 62-63 0.583040146690827 2315 64-65 0.5656384345832706 2315 66-67 0.4998578778681022 2315 68-69 0.5924213995317373 2315 70-71 0.5365408865262609 2315 72-73 0.5545586963568852 2315 74-75 0.24577936485894014 2315 76-77 0.5879678263904466 2315 78-79 0.6316465341659914 2315 80-81 0.6770962151069213 2315 82-83 0.5586430560133664 2315 84-85 0.5396838469090781 2315 86-87 0.5131357397201697 2315 88-89 0.5266189714815503 2315 90-91 0.6577912517836921 2315 92-93 0.8202477947098785 2315 94-95 0.7625864555462449 2315 96-97 0.7538704408558239 2315 98-99 0.8538351695820161 2315 100 0.8746054972651649 2316 1 0.06844978228602372 2316 2 0.009394643471733133 2316 3 -0.02584889111174249 2316 4 -0.10090844260059839 2316 5 -0.06106429722741069 2316 6 -0.2032495502366629 2316 7 -0.11756901204115877 2316 8 -0.02372777033953355 2316 9 -0.05481292436977725 2316 10-11 -0.038372800572062715 2316 12-13 -0.04031351845829789 2316 14-15 -0.1846273998059189 2316 16-17 -0.02885717977900981 2316 18-19 0.0243805362507743 2316 20-21 0.05288422326063369 2316 22-23 0.09470421930623019 2316 24-25 0.01485267626073039 2316 26-27 -0.07184204792962134 2316 28-29 0.008070923252716966 2316 30-31 -0.7110891396129091 2316 32-33 -0.3591692576440977 2316 34-35 -0.5572713412196748 2316 36-37 -0.33766432036404126 2316 38-39 -0.2613352467915462 2316 40-41 -0.022129612702435963 2316 42-43 0.04989427795122481 2316 44-45 -0.10059260148940297 2316 46-47 -0.13339665554580904 2316 48-49 -0.009170777183349799 2316 50-51 0.0899944487516322 2316 52-53 -0.16844458460055023 2316 54-55 -0.12245377517096756 2316 56-57 -0.18353491561533275 2316 58-59 -0.40614684162837733 2316 60-61 0.010304887172630117 2316 62-63 -0.028109354340706716 2316 64-65 -0.28730778363629383 2316 66-67 -0.5421395034626784 2316 68-69 -0.7400694803497387 2316 70-71 -0.5164408430875298 2316 72-73 -0.6198613200959358 2316 74-75 -0.29676072274588705 2316 76-77 -0.0841706529724533 2316 78-79 -0.07083314745895919 2316 80-81 -0.022136338224775898 2316 82-83 0.11267315778829001 2316 84-85 0.10751110518824802 2316 86-87 0.1751225903828022 2316 88-89 0.1405391795968427 2316 90-91 -0.22017581242108264 2316 92-93 -0.2786685155507378 2316 94-95 0.11190704440226185 2316 96-97 0.23093059702843277 2316 98-99 0.36370430072652127 2316 100 0.21605739429288917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 106.0 1 282.0 2 190.0 3 163.0 4 148.0 5 153.0 6 167.0 7 366.0 8 968.0 9 1827.0 10 2924.0 11 4779.0 12 6992.0 13 9054.0 14 10643.0 15 12239.0 16 14574.0 17 18010.0 18 21785.0 19 26129.0 20 31359.0 21 38354.0 22 43354.0 23 45116.0 24 44923.0 25 45800.0 26 50624.0 27 57204.0 28 66816.0 29 79647.0 30 97091.0 31 119284.0 32 150475.0 33 196540.0 34 273971.0 35 380963.0 36 564944.0 37 822571.0 38 749100.0 39 169595.0 40 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56791132893569 18.886850123488262 13.449855589677487 23.095382957898558 2 16.129814120417006 20.009439959140042 40.29923852119502 23.561507399247933 3 19.068428337422823 25.923790250053624 29.621548260628266 25.386233151895286 4 13.119205850604517 16.55561601909098 37.51532061290627 32.80985751739823 5 13.656078868272893 37.28724748582973 34.02116469549689 15.035508950400489 6 30.872415420428794 36.144955340370025 18.320839284503034 14.661789954698149 7 27.697383675900245 32.45151579002058 22.033580312481057 17.81752022159812 8 26.36749617522692 34.67900888049655 20.15956657462198 18.793928369654548 9 27.0784481510502 16.107501186873822 20.506678480089622 36.30737218198636 10-11 25.392652748464016 25.88208951257269 27.742887203104594 20.9823705358587 12-13 25.8889749774193 23.718569953397953 27.878662231537366 22.513792837645383 14-15 23.491979852621157 25.079583397014883 25.760995002596765 25.6674417477672 16-17 22.608830338349712 27.786984673554983 26.62273091373627 22.981454074359036 18-19 22.347626077914445 26.914102714728784 28.75523211424736 21.983039093109415 20-21 23.363971079032826 26.17159190157115 28.548558846528806 21.915878172867217 22-23 23.367210115143696 25.87555042303783 28.35568780795457 22.4015516538639 24-25 22.91481591571438 26.016450968096372 28.562069811298286 22.50666330489096 26-27 22.666803789151327 26.20081106746847 28.413222216007604 22.719162927372594 28-29 22.50752392845584 26.3905842258433 28.574450840119393 22.527441005581473 30-31 23.104948659331185 26.173842910638754 28.486590425388613 22.234618004641444 32-33 23.020347924053787 26.36909168664226 28.153401478843193 22.457158910460766 34-35 23.06310932692244 26.43561075957169 28.29471744789396 22.20656246561191 36-37 23.022194151109467 26.304602226065434 28.070989823807402 22.6022137990177 38-39 22.99280971462431 26.414289698494365 28.17225175463673 22.420648832244595 40-41 22.979296432560744 25.986703905897535 28.472074966944987 22.56192469459673 42-43 22.809480715549665 26.2408504344931 28.061838890669005 22.887829959288222 44-45 22.659168439124073 26.506332192537528 28.152573550416914 22.681925817921485 46-47 22.7176254772081 26.256810879268137 27.805142847588943 23.220420795934817 48-49 22.92296620773712 26.27883365025746 27.758451734334148 23.039748407671276 50-51 22.863226455014875 26.49848288924953 27.64472028980923 22.993570365926367 52-53 22.720480177855766 26.711481053205045 27.48090308330566 23.08713568563353 54-55 22.721956617173923 26.5666588488577 27.565053191169486 23.146331342798895 56-57 22.871285878293897 26.55757817043328 27.305818046450312 23.265317904822503 58-59 22.627871637753337 26.52949342555193 27.690476894954365 23.152158041740368 60-61 22.87046649822287 26.7038817763187 27.477574236181805 22.94807748927662 62-63 22.8830444251704 26.63104644056539 27.42409402546523 23.061815108798974 64-65 22.603898173595617 26.43781986132076 27.53254391609485 23.425738048988773 66-67 22.802450049204488 26.31503237441309 27.345488161214643 23.53702941516778 68-69 22.45737427225064 26.484249051909607 27.544460023596862 23.513916652242887 70-71 22.685188080874077 26.233725181518263 27.563531993450862 23.517554744156797 72-73 22.75664959261893 26.632603572559248 27.199458429040057 23.41128840578176 74-75 22.86713039437061 26.718802958062206 27.093732507677682 23.320334139889507 76-77 22.742459067460963 26.570915856916226 27.11555646964443 23.571068605978382 78-79 22.653202304760157 26.517396065125197 27.200810546535514 23.62859108357913 80-81 22.663512309199202 26.79845828132505 27.017425347133887 23.520604062341864 82-83 23.029248465339 26.53196080832175 27.289554971762815 23.149235754576438 84-85 23.023757003034863 26.728477767525643 26.88788901522628 23.35987621421321 86-87 22.709670388831917 26.926388306536293 27.09603032387144 23.267910980760355 88-89 22.8325494036866 26.43778374897122 27.137245788227602 23.592421059114574 90-91 23.149560738186004 26.368424155008956 27.09516998463817 23.38684512216687 92-93 23.198865116901285 26.51551333321624 26.9383155137663 23.347306036116173 94-95 22.877355114026336 26.38851376510138 27.092273393718607 23.641857727153678 96-97 22.93430507831067 26.364585540108482 27.056955039471426 23.644154342109427 98-99 22.88963546772426 26.46767950496722 26.815033569578855 23.827651457729665 100 23.209720748045402 26.416186738354792 26.524975571261738 23.849116942338068 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1644.0 1 1151.0 2 1495.5 3 2053.5 4 1396.0 5 1112.5 6 1556.5 7 2670.5 8 4272.5 9 5237.5 10 4600.0 11 3655.0 12 3616.0 13 3947.0 14 4264.5 15 4612.0 16 5083.5 17 5454.5 18 5666.5 19 5951.5 20 6423.0 21 6886.0 22 7588.0 23 8592.0 24 9986.5 25 11793.5 26 13934.5 27 16568.5 28 20092.5 29 25114.0 30 31750.5 31 38435.5 32 47566.0 33 58819.0 34 69906.5 35 80847.0 36 93999.5 37 108730.5 38 116624.5 39 119916.0 40 120833.0 41 121226.0 42 124104.0 43 130067.0 44 137815.0 45 147489.0 46 156855.5 47 163685.5 48 169679.5 49 173056.0 50 173041.0 51 172029.0 52 167588.0 53 161521.0 54 155983.0 55 144474.0 56 131192.5 57 116867.0 58 102070.5 59 88976.5 60 74081.0 61 59711.0 62 48445.5 63 38501.5 64 29046.5 65 21420.0 66 16033.5 67 12305.0 68 9528.5 69 7170.5 70 5458.0 71 4197.5 72 3284.5 73 2529.0 74 1935.0 75 1489.0 76 1071.5 77 722.0 78 485.5 79 329.0 80 226.0 81 152.0 82 107.0 83 72.0 84 54.0 85 51.5 86 37.5 87 27.5 88 26.0 89 17.0 90 15.5 91 13.5 92 6.5 93 7.5 94 8.5 95 6.0 96 3.5 97 2.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02490788170968085 2 0.11172483234062443 3 0.01678156509011316 4 0.017142200443052553 5 0.01916175841951316 6 0.045199630901737446 7 0.04544005447036371 8 0.03757820377628491 9 0.03231292762336975 10-11 0.03571492111943137 12-13 0.03513790455472834 14-15 0.030449644966516212 16-17 0.03636406475472228 18-19 0.03535428576649197 20-21 0.04632962167428088 22-23 0.04701482884486573 24-25 0.06713828153888395 26-27 0.05910813434676676 28-29 0.05023650466445765 30-31 0.048036629011527346 32-33 0.04212220922332128 34-35 0.043721025954685924 36-37 0.03922510522137481 38-39 0.04427400016252633 40-41 0.053866900550714227 42-43 0.055249336070315236 44-45 0.05848303306833848 46-47 0.05927643084480515 48-49 0.058723456636964745 50-51 0.05401115469188998 52-53 0.0649023423506597 54-55 0.051715109611509165 56-57 0.055381569033059685 58-59 0.054023175870321294 60-61 0.04938300099583442 62-63 0.05588645852717483 64-65 0.0541193452977718 66-67 0.052532549744838467 68-69 0.05812239771539908 70-71 0.058759520172258675 72-73 0.06082716286244454 74-75 0.05157085547033342 76-77 0.05243638031738796 78-79 0.06309916558596272 80-81 0.06098343818205161 82-83 0.05334998987816777 84-85 0.05932451555853039 86-87 0.05618698798795766 88-89 0.06498649059967888 90-91 0.07022772439573143 92-93 0.07518044990943244 94-95 0.07911137525647184 96-97 0.0743269462408092 98-99 0.08366740188193952 100 0.1299970235562204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4159326.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.03242993565463 #Duplication Level Percentage of deduplicated Percentage of total 1 77.09843982497816 47.82603566591243 2 13.901285299740405 17.246610127433843 3 4.223841896305457 7.860455294755524 4 1.7333864466642652 4.301046932164575 5 0.9171315895604955 2.8445950535593494 6 0.5050009118067842 1.8795860207457624 7 0.3332584743949595 1.4470983074357935 8 0.23711578800047128 1.1767094804621416 9 0.17213163189562797 0.9609969055747892 >10 0.8181723737386644 8.719388039119437 >50 0.03786938643547825 1.5951862283434128 >100 0.02090167348973453 2.264575387776533 >500 0.0010069833122016828 0.4025625373359544 >1k 3.2040377421362905E-4 0.43061527231635444 >5k 4.577196774480415E-5 0.20091215000069396 >10k+ 9.15439354896083E-5 0.8436265970634 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16286 0.3915538238647319 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13426 0.32279268323762067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7076 0.1701237171599437 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.40423568626263E-5 2 0.0 0.0 0.0 0.0 2.40423568626263E-5 3 0.0 0.0 0.0 0.0 2.40423568626263E-5 4 0.0 0.0 0.0 0.0 2.40423568626263E-5 5 0.0 0.0 0.0 0.0 2.40423568626263E-5 6 0.0 0.0 0.0 0.0 2.40423568626263E-5 7 0.0 0.0 0.0 0.0 2.40423568626263E-5 8 0.0 0.0 0.0 0.0 2.40423568626263E-5 9 0.0 0.0 0.0 2.40423568626263E-5 4.80847137252526E-5 10-11 2.40423568626263E-5 0.0 0.0 2.40423568626263E-5 4.80847137252526E-5 12-13 4.80847137252526E-5 3.606353529393945E-5 0.0 2.40423568626263E-5 4.80847137252526E-5 14-15 4.80847137252526E-5 4.80847137252526E-5 0.0 2.40423568626263E-5 4.80847137252526E-5 16-17 4.80847137252526E-5 4.80847137252526E-5 0.0 2.40423568626263E-5 7.212707058787891E-5 18-19 6.0105892156565754E-5 4.80847137252526E-5 0.0 6.0105892156565754E-5 7.212707058787891E-5 20-21 7.212707058787891E-5 4.80847137252526E-5 0.0 2.0436003333232356E-4 7.212707058787891E-5 22-23 7.212707058787891E-5 4.80847137252526E-5 0.0 5.169106725464654E-4 1.0819060588181836E-4 24-25 8.414824901919205E-5 4.80847137252526E-5 0.0 0.0014064778764636386 1.2021178431313151E-4 26-27 9.61694274505052E-5 4.80847137252526E-5 0.0 0.003221675819591924 1.4425414117575782E-4 28-29 9.61694274505052E-5 4.80847137252526E-5 0.0 0.007657490660746476 1.4425414117575782E-4 30-31 9.61694274505052E-5 4.80847137252526E-5 0.0 0.021878544744989934 1.682964980383841E-4 32-33 9.61694274505052E-5 7.212707058787891E-5 0.0 0.05499689132325766 2.4042356862626302E-4 34-35 1.0819060588181836E-4 7.212707058787891E-5 0.0 0.10454818881713046 2.4042356862626302E-4 36-37 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.18452508892065683 2.4042356862626302E-4 38-39 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.2987383051965631 2.644659254888893E-4 40-41 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.42214772297242387 2.8850828235151563E-4 42-43 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.5409289870522291 2.8850828235151563E-4 44-45 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.693670080200494 2.8850828235151563E-4 46-47 1.2021178431313151E-4 7.212707058787891E-5 0.0 0.8733746765701944 3.125506392141419E-4 48-49 1.2021178431313151E-4 7.212707058787891E-5 0.0 1.060352085890839 3.125506392141419E-4 50-51 1.2021178431313151E-4 7.212707058787891E-5 0.0 1.2503107474624495 3.125506392141419E-4 52-53 1.2021178431313151E-4 9.61694274505052E-5 0.0 1.4427337506124789 3.125506392141419E-4 54-55 1.2021178431313151E-4 1.682964980383841E-4 0.0 1.6614470709917906 3.125506392141419E-4 56-57 1.2021178431313151E-4 1.682964980383841E-4 0.0 1.9101892950925223 3.125506392141419E-4 58-59 1.2021178431313151E-4 1.923388549010104E-4 0.0 2.193612618967592 3.4861417450808135E-4 60-61 1.2021178431313151E-4 1.923388549010104E-4 0.0 2.466012522221148 3.606353529393945E-4 62-63 1.2021178431313151E-4 1.923388549010104E-4 0.0 2.747464372833483 4.087200666646471E-4 64-65 1.2021178431313151E-4 2.163812117636367E-4 0.0 3.033868948959519 4.087200666646471E-4 66-67 1.2021178431313151E-4 2.4042356862626302E-4 0.0 3.361349891785352 4.087200666646471E-4 68-69 1.2021178431313151E-4 2.4042356862626302E-4 0.0 3.716912307426732 4.087200666646471E-4 70-71 1.2021178431313151E-4 2.4042356862626302E-4 0.0 4.087441090215098 4.327624235272734E-4 72-73 1.2021178431313151E-4 2.4042356862626302E-4 0.0 4.449134306856447 4.5680478038989973E-4 74-75 1.2021178431313151E-4 2.4042356862626302E-4 0.0 4.811693048344852 4.5680478038989973E-4 76-77 1.2021178431313151E-4 2.4042356862626302E-4 0.0 5.215183902391878 4.5680478038989973E-4 78-79 1.2021178431313151E-4 2.4042356862626302E-4 0.0 5.6469125045740585 4.5680478038989973E-4 80-81 1.2021178431313151E-4 2.8850828235151563E-4 0.0 6.088462409534622 5.048894941151523E-4 82-83 1.2021178431313151E-4 2.8850828235151563E-4 0.0 6.539220537173571 6.010589215656575E-4 84-85 1.2021178431313151E-4 2.8850828235151563E-4 0.0 6.9900147283478145 6.010589215656575E-4 86-87 1.2021178431313151E-4 3.125506392141419E-4 0.0 7.46308416315528 6.010589215656575E-4 88 1.2021178431313151E-4 3.125506392141419E-4 0.0 7.8311966890789515 6.010589215656575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7855 0.0 52.101547 1 GTATCAA 14295 0.0 41.16714 1 ATCAACG 17335 0.0 33.07227 3 CAACGCA 17420 0.0 33.04498 5 TATCAAC 17660 0.0 32.922653 2 TCAACGC 18110 0.0 31.91607 4 AACGCAG 18520 0.0 31.360992 6 TACCTGG 3205 0.0 30.821827 2 GTGGTAT 3425 0.0 28.155993 1 ACGCAGA 20715 0.0 27.698992 7 CGCAGAG 21140 0.0 27.096365 8 GTACATG 18635 0.0 25.697798 1 TACATGG 19225 0.0 24.44366 2 GCAGAGT 23200 0.0 24.164646 9 ACCTGGG 4020 0.0 23.963804 3 TGGTATC 4005 0.0 23.72553 2 ACATGGG 19210 0.0 23.557375 3 GTACCTG 4485 0.0 22.65525 1 GAGTACT 12765 0.0 22.013418 12-13 CATGGGG 13170 0.0 21.943813 4 >>END_MODULE