FastQCFastQC Report
Mon 6 Jun 2016
ERR1211304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4159326
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141560.3403436037473379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126170.30334241653575605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.1643535515129134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79650.051.6705321
GTATCAA138200.039.1177251
TACCTGG36300.031.5334362
ATCAACG170550.030.8932173
CAACGCA171700.030.653085
TATCAAC178350.029.8545742
TCAACGC179550.029.3446874
AACGCAG183300.029.097096
GTGGTAT37900.028.9738221
GTACTAG14850.028.441021
ACCTGGG40650.027.1232933
TGGTATC40200.026.8404562
ACGCAGA205000.025.719597
GTACCTG47500.025.3903471
CGCAGAG211500.024.9956918
GTACATG183300.023.6814581
GCAGAGT229650.022.8559639
TACATGG188400.022.7092112
ACATGGG188200.022.1873533
GAGTACT126200.020.84859312-13
Produced by FastQC (version 0.11.5)