##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4159326 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15159932162086 33.0 31.0 34.0 30.0 34.0 2 32.28019515661912 34.0 31.0 34.0 30.0 34.0 3 32.34196550114129 34.0 31.0 34.0 30.0 34.0 4 35.975267387071845 37.0 35.0 37.0 35.0 37.0 5 35.88112424945773 37.0 35.0 37.0 35.0 37.0 6 35.89815513378851 37.0 35.0 37.0 35.0 37.0 7 35.81835903220858 37.0 35.0 37.0 35.0 37.0 8 35.80113316436365 37.0 35.0 37.0 33.0 37.0 9 37.4246606301117 39.0 37.0 39.0 34.0 39.0 10-11 37.37561518380622 39.0 37.0 39.0 34.0 39.0 12-13 37.30333832933509 39.0 37.0 39.0 34.0 39.0 14-15 38.54361644170233 40.0 38.0 41.0 34.0 41.0 16-17 38.51843339521836 40.0 38.0 41.0 34.0 41.0 18-19 38.524743071353384 40.0 38.0 41.0 34.0 41.0 20-21 38.49710469917481 40.0 38.0 41.0 34.0 41.0 22-23 38.39126832087699 40.0 38.0 41.0 34.0 41.0 24-25 38.29484440507909 40.0 38.0 41.0 34.0 41.0 26-27 38.19360552647232 40.0 38.0 41.0 33.5 41.0 28-29 38.08769413602107 40.0 37.5 41.0 33.0 41.0 30-31 38.09444763406379 40.0 37.0 41.0 33.0 41.0 32-33 38.023185607475824 40.0 37.0 41.0 33.0 41.0 34-35 37.88802272291232 40.0 37.0 41.0 33.0 41.0 36-37 37.74029446116991 40.0 37.0 41.0 33.0 41.0 38-39 37.626942201693254 40.0 37.0 41.0 33.0 41.0 40-41 37.514843390491635 40.0 36.5 41.0 32.0 41.0 42-43 37.40609055409458 40.0 36.0 41.0 32.0 41.0 44-45 37.24744057090019 40.0 36.0 41.0 31.5 41.0 46-47 37.00324752135322 39.0 36.0 41.0 31.0 41.0 48-49 35.45866277372824 39.0 35.0 41.0 24.0 41.0 50-51 36.17216659622256 39.0 35.0 40.0 29.0 41.0 52-53 36.26699938884329 39.0 35.0 40.0 29.5 41.0 54-55 36.096023971191485 38.5 35.0 40.0 29.0 41.0 56-57 35.87731437737749 38.0 35.0 40.0 28.5 41.0 58-59 35.634051165982186 38.0 34.0 40.0 28.0 41.0 60-61 35.37125390027134 37.5 34.0 40.0 28.0 41.0 62-63 35.04707914214947 37.0 34.0 40.0 27.5 41.0 64-65 34.70976776044965 36.5 34.0 39.0 27.0 41.0 66-67 34.36842555741002 36.0 33.0 39.0 26.0 40.5 68-69 34.005599104277955 35.5 33.0 39.0 26.0 40.0 70-71 33.64178991980913 35.0 33.0 38.0 26.0 40.0 72-73 33.26455524284464 35.0 32.0 37.0 26.0 39.5 74-75 32.902441886017115 35.0 32.0 37.0 25.0 39.0 76-77 31.96504048973319 34.0 30.5 36.0 24.5 38.0 78-79 32.3988440675244 35.0 32.0 36.0 25.0 38.0 80-81 32.27993802361247 35.0 32.0 35.5 25.0 37.0 82-83 32.01999662926157 34.0 31.5 35.0 24.5 37.0 84-85 31.75106219132619 34.0 31.0 35.0 24.0 36.0 86-87 31.501902351486756 34.0 31.0 35.0 24.0 36.0 88-89 31.28525643337406 34.0 31.0 35.0 23.0 36.0 90-91 31.073369940225895 34.0 31.0 35.0 23.0 35.0 92-93 30.88642907528768 34.0 30.0 35.0 21.0 35.0 94-95 30.702815311903898 34.0 30.0 35.0 20.0 35.0 96-97 30.51708918704617 34.0 30.0 35.0 20.0 35.0 98-99 30.305538926258727 34.0 29.0 35.0 19.0 35.0 100 27.839829097310478 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25088396411192804 1101 2 -0.24959829599972494 1101 3 -0.2743463140060314 1101 4 -0.2024846842752268 1101 5 -0.21028284562425625 1101 6 -0.18934517567353737 1101 7 -0.2212530170419882 1101 8 -0.15414685240515524 1101 9 -0.28767185976357723 1101 10-11 -0.2621730827157265 1101 12-13 -0.35084870580000427 1101 14-15 -0.4590031147372642 1101 16-17 -0.4479832757089426 1101 18-19 -0.4108844689971036 1101 20-21 -0.496789151234843 1101 22-23 -0.5492647877122963 1101 24-25 -0.530937078047323 1101 26-27 -0.5956821530410892 1101 28-29 -0.5587777025656493 1101 30-31 -0.6085271759424771 1101 32-33 -0.4923877662738718 1101 34-35 -0.5033233589095616 1101 36-37 -0.5400765272050592 1101 38-39 -0.5117677739472555 1101 40-41 -0.517934096896056 1101 42-43 -0.6940430711697871 1101 44-45 -0.6482938655536117 1101 46-47 -0.7406499501275476 1101 48-49 0.42895325710322396 1101 50-51 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0.3311017134840739 2311 3 0.38835942474356244 2311 4 0.2675579008287272 2311 5 0.33125914997289385 2311 6 0.3675258584613488 2311 7 0.3857691336514151 2311 8 0.1851187467464328 2311 9 0.5029821522009001 2311 10-11 0.35820315469551645 2311 12-13 0.4466353387488837 2311 14-15 0.4072086289215733 2311 16-17 0.5344028130256859 2311 18-19 0.6095112288188389 2311 20-21 0.6518174312224048 2311 22-23 0.6482673548092635 2311 24-25 0.5917677276541724 2311 26-27 0.6256073435312715 2311 28-29 0.7057310859370958 2311 30-31 0.55607899124265 2311 32-33 0.5567938892119244 2311 34-35 0.6615056665961632 2311 36-37 0.7056766802573122 2311 38-39 0.7556470980072305 2311 40-41 0.6767120577520629 2311 42-43 0.7063693755167009 2311 44-45 0.7250619930795281 2311 46-47 0.7661925669033209 2311 48-49 -6.138472719437846 2311 50-51 -1.143783936053218 2311 52-53 0.27143076655541876 2311 54-55 0.7717066367610244 2311 56-57 0.8602350748586858 2311 58-59 0.8960085303574274 2311 60-61 0.7905706208816454 2311 62-63 0.6804929560072495 2311 64-65 0.7402039492414616 2311 66-67 0.8583143299297689 2311 68-69 0.9024547706473172 2311 70-71 0.8927852774135943 2311 72-73 0.386474496940977 2311 74-75 0.6309578385091683 2311 76-77 0.7716773448685821 2311 78-79 0.6421321807352598 2311 80-81 0.7340839858415436 2311 82-83 0.6879483140886578 2311 84-85 0.6926588411519781 2311 86-87 0.7942224772411208 2311 88-89 0.7783041201511729 2311 90-91 0.8656255524277654 2311 92-93 0.931907399224805 2311 94-95 0.9884270864504714 2311 96-97 0.9503721353506052 2311 98-99 0.899985919776757 2311 100 1.1133694638880485 2312 1 0.05663971633922671 2312 2 0.15124433701966922 2312 3 0.13645738477443103 2312 4 -0.005648933854615734 2312 5 0.18183115238799274 2312 6 0.096683210992758 2312 7 0.25240037438599217 2312 8 0.31813805999697564 2312 9 0.27858531456156754 2312 10-11 0.24072235769030215 2312 12-13 0.26084709204092604 2312 14-15 0.29002783285728384 2312 16-17 0.4093658631388095 2312 18-19 0.22287974679787936 2312 20-21 0.326526068613056 2312 22-23 0.5390546685122004 2312 24-25 0.630373081249104 2312 26-27 0.5279846710713727 2312 28-29 0.6651796040428408 2312 30-31 0.6862874580093674 2312 32-33 0.6417688238433428 2312 34-35 0.30093457995135253 2312 36-37 0.5668092700994478 2312 38-39 0.6198559027611807 2312 40-41 0.41051720065461694 2312 42-43 0.4949219535404694 2312 44-45 0.5994417636674285 2312 46-47 0.624869998047501 2312 48-49 -4.842505942242148 2312 50-51 -1.0374736033975651 2312 52-53 0.3972593500097119 2312 54-55 0.6830379012422796 2312 56-57 0.7941134328033854 2312 58-59 0.8318947356056796 2312 60-61 0.8566211812762319 2312 62-63 0.557889970745201 2312 64-65 0.7918915890350107 2312 66-67 0.7294325888520277 2312 68-69 0.6576259614644968 2312 70-71 0.7008201927626061 2312 72-73 0.8172152936126835 2312 74-75 0.5101714182179364 2312 76-77 0.7132726045882087 2312 78-79 0.3249082605546576 2312 80-81 0.5165064693341606 2312 82-83 0.555414647885712 2312 84-85 0.669079153812536 2312 86-87 0.8364176643139842 2312 88-89 0.7742854211095214 2312 90-91 0.5288372901619631 2312 92-93 0.8082061417501656 2312 94-95 0.9784669201286995 2312 96-97 0.987332176743795 2312 98-99 1.013165111799065 2312 100 0.6069835266167303 2313 1 0.07346815993473399 2313 2 0.20094143519506247 2313 3 0.3231379424218872 2313 4 0.05317121423682636 2313 5 0.1209120712412215 2313 6 0.2632422124897218 2313 7 0.16492348807277324 2313 8 0.25071696369231944 2313 9 0.4358598027126561 2313 10-11 0.17570131366904462 2313 12-13 -0.01969806579759137 2313 14-15 0.08055315147181119 2313 16-17 0.07440309011607837 2313 18-19 0.38898055919967334 2313 20-21 0.3144062885602992 2313 22-23 0.4155073187893237 2313 24-25 0.23382118588216372 2313 26-27 0.4068712177946878 2313 28-29 0.3752758372538878 2313 30-31 0.4802917002687721 2313 32-33 0.4576410863162437 2313 34-35 0.4045300062342534 2313 36-37 -0.32354857139627313 2313 38-39 0.19284634323827277 2313 40-41 0.3886653130542186 2313 42-43 0.45910169437426873 2313 44-45 0.5106418886642814 2313 46-47 0.4442290330819958 2313 48-49 -5.420675226122089 2313 50-51 -1.3443123347947719 2313 52-53 0.08193404032875407 2313 54-55 0.3309064123448806 2313 56-57 0.39554528116286036 2313 58-59 0.46057424712754624 2313 60-61 0.7698113155860753 2313 62-63 0.788894711262067 2313 64-65 0.8781062413927572 2313 66-67 0.9215187160450284 2313 68-69 0.9108645091741749 2313 70-71 0.938781041450639 2313 72-73 0.6620917518922127 2313 74-75 0.8136158249357095 2313 76-77 0.8466025191805535 2313 78-79 0.8134149522023861 2313 80-81 -0.3250842447612037 2313 82-83 0.22381288169180635 2313 84-85 0.6965857625775378 2313 86-87 0.8232289899484257 2313 88-89 0.8402561840548906 2313 90-91 0.8648924813645493 2313 92-93 0.9061850845939894 2313 94-95 0.7537011302020531 2313 96-97 0.741581907489337 2313 98-99 1.0392923020530063 2313 100 1.2333499646671058 2314 1 0.008149681322166202 2314 2 0.16821621113159324 2314 3 0.19160948758393204 2314 4 0.08107028413087392 2314 5 0.16510658492686758 2314 6 0.2718841390034683 2314 7 0.03872240360044543 2314 8 0.21555166573485707 2314 9 0.37060934087518405 2314 10-11 0.2544587324882528 2314 12-13 0.17745277714373486 2314 14-15 0.13419873673427674 2314 16-17 0.3881868258001262 2314 18-19 0.4946427435921166 2314 20-21 0.4983648246767416 2314 22-23 0.2579953617037489 2314 24-25 0.37627367964822866 2314 26-27 0.5471455893373331 2314 28-29 0.6008523553443155 2314 30-31 0.4569343020377019 2314 32-33 0.4842365385708618 2314 34-35 0.3259526554630767 2314 36-37 0.4448805916780785 2314 38-39 0.522057047194032 2314 40-41 0.26550515453896395 2314 42-43 0.24780867672273388 2314 44-45 0.5536677687569238 2314 46-47 0.5499645934176343 2314 48-49 -6.220131627838185 2314 50-51 -1.6029520841464873 2314 52-53 -0.9417087336034413 2314 54-55 -0.41309872173815165 2314 56-57 -0.1724541882794952 2314 58-59 0.3710599730949653 2314 60-61 0.585702253835727 2314 62-63 0.45303303407021644 2314 64-65 0.6373752463984488 2314 66-67 0.7725120936763545 2314 68-69 0.7878741692979716 2314 70-71 0.5544080406715537 2314 72-73 0.7471790181292022 2314 74-75 0.7611925319368211 2314 76-77 0.6901806168857796 2314 78-79 0.5865985012533983 2314 80-81 0.6300938839378745 2314 82-83 0.5554826399285702 2314 84-85 0.5746345877250754 2314 86-87 0.6291017896470663 2314 88-89 0.5991394629368472 2314 90-91 0.8573168060033005 2314 92-93 0.8311482012360578 2314 94-95 0.8970346712031478 2314 96-97 0.8405324930566067 2314 98-99 0.9290759412720213 2314 100 -0.4344168052305335 2315 1 0.06369802326335616 2315 2 0.17640024027753753 2315 3 0.274618737171167 2315 4 0.13546377195602588 2315 5 0.1947180017718182 2315 6 0.2518549877740881 2315 7 0.3251320422821067 2315 8 0.21077194836162505 2315 9 0.41808758764073417 2315 10-11 0.15179051790629217 2315 12-13 0.3769410218906515 2315 14-15 0.17771739237513628 2315 16-17 0.3515876424224018 2315 18-19 0.42794000150777833 2315 20-21 0.3285684116795409 2315 22-23 0.3981832227333939 2315 24-25 0.44384269454470626 2315 26-27 0.5208552485579645 2315 28-29 0.5158381764049551 2315 30-31 0.4940880816043389 2315 32-33 0.6322716293522106 2315 34-35 0.6744098969475587 2315 36-37 0.5232037833454299 2315 38-39 0.6359885272602099 2315 40-41 0.5939248925745702 2315 42-43 0.6942584986155111 2315 44-45 0.6668865554769354 2315 46-47 0.5574831556194155 2315 48-49 -4.809650539995751 2315 50-51 -0.9446824612789442 2315 52-53 0.16339685255487524 2315 54-55 0.10010477094407122 2315 56-57 0.49475438096547464 2315 58-59 0.6587049469099853 2315 60-61 0.5474821889396466 2315 62-63 0.31500304277063407 2315 64-65 0.49464967697100803 2315 66-67 -0.18688263265733696 2315 68-69 0.5004638289417187 2315 70-71 0.5066969014111891 2315 72-73 0.5725224948915226 2315 74-75 0.8412983164328054 2315 76-77 0.8309910818862285 2315 78-79 0.692523508459665 2315 80-81 0.5845693954066462 2315 82-83 0.5968090473106074 2315 84-85 0.6059910895422895 2315 86-87 0.5317793922118739 2315 88-89 0.571681398130675 2315 90-91 0.6932157692702852 2315 92-93 0.7583887116602455 2315 94-95 0.35197559302373094 2315 96-97 0.4184175347824457 2315 98-99 0.3487864520223667 2315 100 0.4727745659350475 2316 1 0.22772737130947718 2316 2 0.24796829768152406 2316 3 0.13001332496764917 2316 4 -0.03143319116294663 2316 5 0.12037161385559614 2316 6 0.140214184772951 2316 7 0.24353913336269528 2316 8 0.28415270200428466 2316 9 0.42542304864280567 2316 10-11 0.4339644440580983 2316 12-13 0.3287686634947491 2316 14-15 0.4837512068027934 2316 16-17 0.446019890816963 2316 18-19 0.14993766279497578 2316 20-21 0.4444721109805059 2316 22-23 0.5408461465981773 2316 24-25 0.556153239426024 2316 26-27 0.6016416030516893 2316 28-29 0.6678141518377032 2316 30-31 0.6441374185383424 2316 32-33 0.5801938789958072 2316 34-35 0.5442398987995816 2316 36-37 0.46344377059664055 2316 38-39 0.48452958825194514 2316 40-41 0.1672158232789016 2316 42-43 0.08531163003566178 2316 44-45 0.37904241367255054 2316 46-47 0.4214537774241478 2316 48-49 -5.087562108225438 2316 50-51 -1.1119809249642927 2316 52-53 0.2858666926351603 2316 54-55 0.5059128112922551 2316 56-57 0.3828779838752965 2316 58-59 0.29376866929659684 2316 60-61 0.45827605918631065 2316 62-63 0.4218156097986494 2316 64-65 0.4604381203702843 2316 66-67 0.5885960282034617 2316 68-69 0.31207364292144746 2316 70-71 -0.10052270070615066 2316 72-73 -0.0012985009780379642 2316 74-75 0.17361194083466813 2316 76-77 0.2733274473628704 2316 78-79 0.3061513559394129 2316 80-81 0.17830173123374493 2316 82-83 0.07132558412057222 2316 84-85 0.2988045007465736 2316 86-87 0.3215376821519662 2316 88-89 0.2369630773523106 2316 90-91 0.18578652286987918 2316 92-93 0.21419920701081807 2316 94-95 0.4807712851405874 2316 96-97 0.6183148984544395 2316 98-99 0.5588834676870036 2316 100 -0.34013650698713604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 5.0 11 6.0 12 23.0 13 97.0 14 383.0 15 1022.0 16 2352.0 17 5036.0 18 8564.0 19 12654.0 20 17982.0 21 24745.0 22 31625.0 23 34299.0 24 35700.0 25 38832.0 26 44526.0 27 54213.0 28 66730.0 29 81378.0 30 99970.0 31 123937.0 32 157060.0 33 203770.0 34 285815.0 35 404877.0 36 631293.0 37 942132.0 38 753805.0 39 96488.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84982151699611 19.53536940799149 14.140981251288148 23.473827823724257 2 15.80589746456388 20.342177486539022 39.8534589811372 23.998466067759892 3 18.685396637866305 25.820500115042762 30.086627078493304 25.407476168597633 4 13.10179164374127 16.858510548247217 37.3462635772852 32.69343423072632 5 13.56441332817222 37.40276632422205 34.09041403502023 14.942406312585502 6 31.123816695301116 36.301314203310824 17.97228685609159 14.602582245296475 7 27.759287865218514 32.48192915917848 21.849434703948354 17.909348271654654 8 26.529889968907394 34.820787134411816 19.92386494671556 18.725457949965232 9 26.95490592065225 16.00381640935799 20.357677770689868 36.683599899299885 10-11 25.459100002668734 25.935011813345533 27.63662884841339 20.969259335572342 12-13 25.76918786282957 23.71786796021082 27.893885109159616 22.61905906779999 14-15 23.420135572625384 25.10077066674713 25.63649519194301 25.842598568684476 16-17 22.604967192286512 27.858925689984986 26.56611556984852 22.96999154787998 18-19 22.344490993129895 26.980736056932315 28.66185018001719 22.0129227699206 20-21 23.270400407758714 26.202019679887773 28.526508824453984 22.001071087899533 22-23 23.236601813809155 25.949369523284254 28.304265632329184 22.509763030577407 24-25 22.85869427627148 26.07838698846724 28.515188340032537 22.547730395228747 26-27 22.67420844342243 26.262366653399276 28.36270972626418 22.700715176914112 28-29 22.528320689926687 26.460561406925354 28.523260923007754 22.487856980140204 30-31 23.074065650093456 26.143452518055142 28.493134019458537 22.289347812392858 32-33 22.98694380361761 26.39954757338257 28.121634857624233 22.491873765375587 34-35 23.047242562673183 26.3883735475892 28.307603407657105 22.25678048208051 36-37 22.97211992549025 26.298906642054533 28.15453221950268 22.574441212952536 38-39 22.958810995614062 26.35117058746223 28.254148497133574 22.43586991979013 40-41 22.952639696203907 26.02003002809138 28.52098846735193 22.50634180835278 42-43 22.767131162804787 26.265512841797655 28.135126528142123 22.832229467255434 44-45 22.57078029552457 26.51804478709557 28.15461426682041 22.75656065055945 46-47 22.67232499156012 26.268374166952807 27.85325577302067 23.206045068466405 48-49 22.989743035102446 26.35037141943308 27.734013586655347 22.925871958809115 50-51 22.794491962034126 26.498739833361267 27.682425983695865 23.02434222090874 52-53 22.664152655708627 26.688501946767396 27.546058305512204 23.101287092011766 54-55 22.622557432312615 26.542892471418345 27.622812907445265 23.211737188823776 56-57 22.86950058012806 26.462207040137457 27.33192425911497 23.33636812061951 58-59 22.544759268578694 26.47259382188527 27.73606740705814 23.246579502477896 60-61 22.768531653482725 26.68889904065783 27.51798466014282 23.024584645716622 62-63 22.77456201262604 26.629661189740673 27.401645193680057 23.194131603953235 64-65 22.473885212162852 26.41829758720389 27.47583843321012 23.631978767423135 66-67 22.593466917452538 26.34414420524231 27.37120469326469 23.691184184040466 68-69 22.28593148523295 26.417661035002432 27.57403546381657 23.72237201594805 70-71 22.46541196614906 26.128304591136132 27.5948901335424 23.811393309172413 72-73 22.504944252114534 26.525852474675137 27.23544643999419 23.73375683321614 74-75 22.529300042582832 26.599122423359916 27.124867725122165 23.746709808935083 76-77 22.347458029579926 26.478788909842123 27.191349768228662 23.982403292349293 78-79 22.25731220112188 26.346537699472982 27.247970390891734 24.1481797085134 80-81 22.19697179161983 26.63927775006161 27.062902343585645 24.100848114732912 82-83 22.46628167159182 26.344245614883015 27.32825416840547 23.8612185451197 84-85 22.47174231628117 26.481565471589853 26.98055088355011 24.06614132857887 86-87 22.046208231630334 26.68344415453245 27.198852910409176 24.071494703428044 88-89 22.11347933235738 26.228501243274067 27.237081626071163 24.42093779829739 90-91 22.322050364556283 26.06481214289837 27.26404840230084 24.34908909024451 92-93 22.30343718177304 26.223211827492886 27.106210107038475 24.367140883695598 94-95 21.866000074055158 26.067632557533404 27.320835803842307 24.745531564569127 96-97 21.888868064772964 26.00791960229599 27.338821042209467 24.764391290721584 98-99 21.71523884193376 26.124854053831786 27.17380287471419 24.986104229520258 100 21.67520408757186 26.05264259192539 27.208886293483005 25.063267027019748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1527.0 1 1117.5 2 1447.5 3 1934.5 4 1287.5 5 957.0 6 1352.5 7 2420.5 8 3853.5 9 4718.5 10 4194.5 11 3338.5 12 3312.5 13 3578.5 14 4019.0 15 4559.5 16 4918.0 17 5224.5 18 5570.0 19 5854.5 20 6207.0 21 6830.5 22 7692.0 23 8630.5 24 9973.5 25 11706.0 26 13742.5 27 16383.5 28 19808.5 29 24865.0 30 31745.5 31 38857.5 32 47727.5 33 58534.0 34 69808.0 35 80829.5 36 93605.0 37 108310.0 38 116609.5 39 119234.0 40 120114.5 41 120500.5 42 123267.5 43 129447.5 44 137294.0 45 147829.5 46 157802.5 47 164318.0 48 170590.5 49 174619.0 50 174660.0 51 173423.0 52 170314.0 53 164882.0 54 158683.5 55 147013.5 56 132829.5 57 117935.5 58 102610.0 59 89158.5 60 73995.5 61 59213.0 62 47448.5 63 37213.0 64 27998.5 65 20441.5 66 15013.0 67 11358.0 68 8656.5 69 6422.0 70 4851.5 71 3689.5 72 2838.5 73 2270.5 74 1698.0 75 1216.0 76 855.0 77 514.5 78 303.0 79 179.0 80 119.5 81 79.5 82 43.0 83 27.5 84 17.5 85 15.0 86 12.5 87 8.0 88 7.0 89 4.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029499971870442468 2 0.0019474309058727303 3 7.21270705878789E-5 4 0.021878544744989934 5 3.8467770980202086E-4 6 0.0 7 2.4042356862626304E-5 8 0.0033178452470424293 9 0.01065076409014345 10-11 0.0011299907725434361 12-13 9.616942745050522E-5 14-15 1.3223296274444464E-4 16-17 3.4861417450808135E-4 18-19 2.4042356862626304E-5 20-21 8.414824901919205E-5 22-23 3.1255063921414194E-4 24-25 1.2021178431313152E-5 26-27 1.3223296274444464E-4 28-29 0.001045842523524244 30-31 0.0029812522509656612 32-33 0.010085768703871732 34-35 0.019450266701864678 36-37 0.022695984878319225 38-39 0.022179074205772763 40-41 0.0053013396882090995 42-43 0.005565805613697988 44-45 0.00919620149995456 46-47 0.011973093717587898 48-49 1.189724008168631 50-51 0.0180077252901071 52-53 0.018404424178340432 54-55 0.009821302778382843 56-57 0.007945998943097993 58-59 0.008535036686232338 60-61 0.005433572650953544 62-63 0.007260791772513142 64-65 0.019486330237158615 66-67 0.028778701164563682 68-69 0.028862849413582876 70-71 0.026350423121438423 72-73 0.0231287473018465 74-75 0.00896779910975961 76-77 0.014269138797968707 78-79 0.008066210727411123 80-81 0.0024282780431252563 82-83 0.01374020694699093 84-85 0.023753848580274784 86-87 0.02346534029792327 88-89 0.032529308835133386 90-91 0.02033983390578185 92-93 0.01300691506268083 94-95 0.0063471822117333425 96-97 0.003666459421550511 98-99 0.016505077986192957 100 0.03731373785079602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4159326.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.24335841190993 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24092490637832 48.077345730151265 2 13.690855051563494 17.043295958799483 3 4.208495019139542 7.8585259155312075 4 1.802104297968707 4.486760948564383 5 0.907488065980948 2.824255247269156 6 0.5265730320350684 1.966540437780294 7 0.3456081115742449 1.5058266691145359 8 0.23618000411167683 1.176050931651957 9 0.1759571035940652 0.9856944957714269 >10 0.8075041445451477 8.611787085183359 >50 0.03760295896432644 1.6010607451626215 >100 0.019286522039147793 2.127005640938364 >500 9.624653046276913E-4 0.3793544059718852 >1k 3.2082176042151953E-4 0.39772590014573816 >5k 4.583168006021707E-5 0.19501178119324816 >10k+ 9.166336012043414E-5 0.7637581067710405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14156 0.3403436037473379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12617 0.30334241653575605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6836 0.1643535515129134 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.40423568626263E-5 2 0.0 0.0 0.0 0.0 2.40423568626263E-5 3 0.0 0.0 0.0 0.0 2.40423568626263E-5 4 0.0 0.0 0.0 0.0 2.40423568626263E-5 5 0.0 0.0 0.0 0.0 2.40423568626263E-5 6 0.0 0.0 0.0 0.0 2.40423568626263E-5 7 0.0 0.0 0.0 0.0 2.40423568626263E-5 8 0.0 0.0 0.0 0.0 2.40423568626263E-5 9 0.0 0.0 0.0 2.40423568626263E-5 9.61694274505052E-5 10-11 0.0 0.0 0.0 2.40423568626263E-5 9.61694274505052E-5 12-13 0.0 0.0 0.0 2.40423568626263E-5 9.61694274505052E-5 14-15 0.0 1.202117843131315E-5 0.0 2.40423568626263E-5 1.5627531960707094E-4 16-17 0.0 2.40423568626263E-5 0.0 2.40423568626263E-5 2.0436003333232356E-4 18-19 0.0 2.40423568626263E-5 0.0 6.0105892156565754E-5 2.163812117636367E-4 20-21 0.0 4.80847137252526E-5 0.0 1.923388549010104E-4 2.163812117636367E-4 22-23 0.0 4.80847137252526E-5 0.0 4.928683156838391E-4 2.6446592548888927E-4 24-25 0.0 4.80847137252526E-5 0.0 0.0014305202333262648 3.125506392141419E-4 26-27 0.0 4.80847137252526E-5 0.0 0.0032577393548858637 3.125506392141419E-4 28-29 0.0 4.80847137252526E-5 0.0 0.007765681266628296 3.125506392141419E-4 30-31 2.40423568626263E-5 4.80847137252526E-5 0.0 0.022118968313616198 3.125506392141419E-4 32-33 4.80847137252526E-5 4.80847137252526E-5 0.0 0.05545369610364756 3.846777098020208E-4 34-35 6.0105892156565754E-5 4.80847137252526E-5 0.0 0.10511318420340218 4.087200666646471E-4 36-37 7.212707058787891E-5 6.0105892156565754E-5 0.0 0.1839240299990912 4.087200666646471E-4 38-39 7.212707058787891E-5 7.212707058787891E-5 0.0 0.29271569480247517 4.087200666646471E-4 40-41 7.212707058787891E-5 7.212707058787891E-5 0.0 0.4098500574371905 4.327624235272734E-4 42-43 7.212707058787891E-5 7.212707058787891E-5 0.0 0.5232097700444736 4.447836019585866E-4 44-45 7.212707058787891E-5 7.212707058787891E-5 0.0 0.6696637868731616 4.5680478038989973E-4 46-47 7.212707058787891E-5 7.212707058787891E-5 0.0 0.8406770712370225 4.688259588212129E-4 48-49 7.212707058787891E-5 7.212707058787891E-5 0.0 1.0207543241380934 5.048894941151523E-4 50-51 8.414824901919205E-5 7.212707058787891E-5 0.0 1.2104004350704898 5.048894941151523E-4 52-53 9.61694274505052E-5 1.0819060588181836E-4 0.0 1.4024147181538549 5.169106725464655E-4 54-55 1.0819060588181836E-4 2.2840239019494986E-4 0.0 1.6205750643253256 5.289318509777786E-4 56-57 1.2021178431313151E-4 2.644659254888893E-4 0.0 1.8684758059358655 5.289318509777786E-4 58-59 1.2021178431313151E-4 3.365929960767682E-4 0.0 2.1514303038521145 5.289318509777786E-4 60-61 1.2021178431313151E-4 3.365929960767682E-4 0.0 2.42272425868999 5.289318509777786E-4 62-63 1.2021178431313151E-4 3.365929960767682E-4 0.0 2.702937927923899 5.289318509777786E-4 64-65 1.2021178431313151E-4 3.365929960767682E-4 0.0 2.987695602604845 5.289318509777786E-4 66-67 1.2021178431313151E-4 3.365929960767682E-4 0.0 3.314214851156173 5.409530294090917E-4 68-69 1.2021178431313151E-4 3.606353529393945E-4 0.0 3.6692122714112814 5.649953862717182E-4 70-71 1.2021178431313151E-4 3.9669888823333396E-4 0.0 4.038635105783966 5.770165647030313E-4 72-73 1.2021178431313151E-4 4.327624235272734E-4 0.0 4.4002201318194345 6.972283490161627E-4 74-75 1.2021178431313151E-4 4.327624235272734E-4 0.0 4.763223656909798 6.972283490161627E-4 76-77 1.2021178431313151E-4 4.327624235272734E-4 0.0 5.166990998060744 7.092495274474758E-4 78-79 1.2021178431313151E-4 4.327624235272734E-4 0.0 5.598767684956649 7.21270705878789E-4 80-81 1.2021178431313151E-4 4.327624235272734E-4 0.0 6.040630140556427 7.332918843101022E-4 82-83 1.2021178431313151E-4 4.327624235272734E-4 0.0 6.491893157689491 7.693554196040416E-4 84-85 1.2021178431313151E-4 4.327624235272734E-4 0.0 6.9432162807147115 7.693554196040416E-4 86-87 1.2021178431313151E-4 4.327624235272734E-4 0.0 7.417343579224134 7.693554196040416E-4 88 1.2021178431313151E-4 4.327624235272734E-4 0.0 7.785684507538 7.693554196040416E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7965 0.0 51.670532 1 GTATCAA 13820 0.0 39.117725 1 TACCTGG 3630 0.0 31.533436 2 ATCAACG 17055 0.0 30.893217 3 CAACGCA 17170 0.0 30.65308 5 TATCAAC 17835 0.0 29.854574 2 TCAACGC 17955 0.0 29.344687 4 AACGCAG 18330 0.0 29.09709 6 GTGGTAT 3790 0.0 28.973822 1 GTACTAG 1485 0.0 28.44102 1 ACCTGGG 4065 0.0 27.123293 3 TGGTATC 4020 0.0 26.840456 2 ACGCAGA 20500 0.0 25.71959 7 GTACCTG 4750 0.0 25.390347 1 CGCAGAG 21150 0.0 24.995691 8 GTACATG 18330 0.0 23.681458 1 GCAGAGT 22965 0.0 22.855963 9 TACATGG 18840 0.0 22.709211 2 ACATGGG 18820 0.0 22.187353 3 GAGTACT 12620 0.0 20.848593 12-13 >>END_MODULE