FastQCFastQC Report
Mon 6 Jun 2016
ERR1211303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627075
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217750.4705996769017144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171100.36978004462862607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105570.22815709708617216No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105490.22798420168248842No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG80790.17460274579512977No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT65760.14212002182804473No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC56890.12295024394460864No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT56210.12148063301329673No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG52440.1133329371146999No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT48480.10477461463235414No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA48330.1044504357504471No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT46840.1012302588568372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG53550.069.22322
ACCTGGG57400.063.9372753
GTACCTG66500.056.66681
GGTATCA104100.045.6896971
TAACGCA4950.045.5670664
CCTGGGG33250.044.5178224
TATCACG2500.043.2785572
GTACACG4650.039.4574931
GTATCAA188200.037.4713941
TATAACG6850.035.7106482
GGATGAC43650.031.7572888
ATCAACG215850.031.566833
CAACGCA217050.031.4995335
TACGTGG4400.031.0048922
TCAACGC220600.030.951034
AACGCAG228650.030.1686446
GTACATG213800.028.6717741
TATCAAC241400.028.2758122
ACGCAGA249100.027.5985077
CGCAGAG251850.027.3152248