FastQCFastQC Report
Mon 6 Jun 2016
ERR1211303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627075
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187780.40582873629668853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157260.33986913979133687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100410.21700534354857007No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84180.18192918852622877No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG81780.1767423264157162No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT63490.13721411474851825No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT54300.11735275525034715No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC50540.10922667127721075No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG49240.10641712096734977No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA49070.1060497182345218No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA48770.10540136047070772No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG46600.10071157264578594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54650.068.422632
ACCTGGG56950.064.020623
GTACCTG68400.055.092731
CCTGGGG30200.048.9436344
GGTATCA98800.046.9758031
TATCACG2450.044.0446662
TAACGCA4050.044.027214
GTATCAA176850.037.7071651
GTACCCG3400.033.1258621
ATCAACG209700.030.5664393
CAACGCA212900.030.101155
TCAACGC215750.029.7093074
GGATGAC40800.029.6665928
AACGCAG223650.028.9270046
TACGTGG4300.028.3685052
TATCAAC234250.027.559442
TATAACG6300.027.5545122
GGGTATG22050.026.8083487
GTACATG210550.026.7684121
GTACACG4050.026.650641
Produced by FastQC (version 0.11.5)